Name: Ammonifex thiophilus SR
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 16790

Type strain:

Species: Ammonifex thiophilus

Strain Designation: SR

Culture col. no.: DSM 19636, VKM B-2461

Strain history: <- M. Miroshnichenko; SR

NCBI tax ID(s): 444093 (species)

SI-ID 396578

DSM 19636

Section

Ammonifex thiophilus SR is an anaerobe, spore-forming, thermophilic bacterium that was isolated from Treshchinnyi hot spring.

Gram-positive
motile
rod-shaped
spore-forming
anaerobe
thermophilic
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Bacillota*
	Class	*Clostridia*
	Order	*Thermoanaerobacterales*
	Family	*Thermoanaerobacteraceae*
	Genus	*Ammonifex*
	Species	**Ammonifex thiophilus**
	Full Scientific Name (LPSN)	Ammonifex thiophilus Miroshnichenko et al. 2008

Information on morphological and physiological properties Morphology

[Ref.: #31184]	Gram stain	positive

[Ref.: #31184]	Cell length	03-06 µm

[Ref.: #31184]	Cell width	0.6-0.7 µm

[Ref.: #31184]	Cell shape	rod-shaped

[Ref.: #31184]	Motility	yes

[Ref.: #31184]

Pigment production

yes

Information on culture and growth conditions Culture and growth conditions

[Ref.: #8226]

Culture medium

AMMONIFEX THIOPHILUS MEDIUM (DSMZ Medium 503c)

[Ref.: #8226]

Culture medium growth

[Ref.: #8226]

Culture medium link

Medium recipe at MediaDive

[Ref.: #8226]

Culture medium composition

expand / minimize

		Temperature
[Ref.: #8226]	growth	70 °C
[Ref.: #31184]	growth	60-82 °C
[Ref.: #31184]	optimum	75 °C

[Ref.: #8226]	Temperature range	thermophilic
[Ref.: #31184]	Temperature range	hyperthermophilic
[Ref.: #31184]	Temperature range	thermophilic

	pH	Kind of pH		pH
[Ref.: #31184]			optimum	6.8-7
[Ref.: #31184]			growth	6-7.5

Information on physiology and metabolism Physiology and metabolism

[Ref.: #8226]	Oxygen tolerance	anaerobe
[Ref.: #31184]	Oxygen tolerance	anaerobe

[Ref.: #31184]

Ability of spore formation

yes

[Ref.: #31184]

Observation

aggregates in chains

	Metabolite utilization	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #31184]		formate ChEBI	+	carbon source

	Metabolite production	Metabolite	Production
[Ref.: #31184]		hydrogen sulfide ChEBI	yes

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #8226]	Sample type/isolated from	Treshchinnyi hot spring
[Ref.: #8226]	Geographic location (country and/or sea, region)	Kamchatka, Uzon Caldera,
[Ref.: #8226]	Country	Russia
[Ref.: #8226]	Country ISO 3 Code	RUS
[Ref.: #8226]	Continent	Asia
* marker position based on {}

Isolation sources categories

#Environmental	#Aquatic	#Thermal spring
#Condition	#Thermophilic (>45°C)	-

What are isolation sources categories?

[Ref.: #69479]

Global distribution of 16S sequence EF554597 (>99% sequence identity) for Ammonifex thiophilus from Microbeatlas

Aquatic Samples	1
Soil Samples	1
Animal Samples
Plant Samples
Total Samples	2

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #8226]

Biosafety level

Risk group (German classification)
According to TRBA 466

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #8226]	Ammonifex thiophilus strain SR 16S ribosomal RNA gene, partial sequence	EF554597	1536	ENA	444093 tax ID

Genome sequence information:

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #66792]	Ammonifex thiophilus SR	GCA_003368535	contig	GenBank	444093 tax ID	*
[Ref.: #66792]	Ammonifex thiophilus SR	2831524987	draft	JGI IMG/M	444093 tax ID	*

[Ref.: #8226]

GC-content

56.2 mol%

Availability in culture collections External links

[Ref.: #8226]

Culture collection no.

DSM 19636, VKM B-2461

[Ref.: #85806]

SI-ID 396578

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Ammonifex thiophilus sp. nov., a hyperthermophilic anaerobic bacterium from a Kamchatka hot spring.	Miroshnichenko ML, Tourova TP, Kolganova TV, Kostrikina NA, Chernych N, Bonch-Osmolovskaya EA	Int J Syst Evol Microbiol	10.1099/ijs.0.2008/000182-0	2008	*

References

#8226

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 19636

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#31184

Barberan A, Caceres Velazquez H, Jones S, Fierer N.: Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information. mSphere 2: 2017 ( DOI 10.1128/mSphere.00237-17 , PubMed 28776041 ) - originally annotated from #27511 (see below)

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

#85806

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID396578.1 )

#27511

IJSEM 2935 2008 ( DOI 10.1099/ijs.0.2008/000182-0 , PubMed 19060086 )

* These data were automatically processed and therefore are not curated

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