Thermoflavimicrobium dichotomicum (DSM 44778, ATCC 49854, JCM 9688)

Cell morphology

Multimedia

• @ref:	12083
  multimedia content:	DSM_44778.jpg
  multimedia.multimedia content:	https://www.dsmz.de/microorganisms/photos/DSM_44778.jpg
  caption:	Medium 553 45°C
  intellectual property rights:	© Leibniz-Institut DSMZ
  manual_annotation:	1

Culture medium

@ref	Name	Growth	Medium link	Composition
12083	GYM+S MEDIUM (DSMZ Medium 214)		Medium recipe at MediaDive	Name: GYM+S MEDIUM (DSMZ Medium 214) Composition: Starch 20.0 g/l Agar 12.0 g/l Malt extract 10.0 g/l Yeast extract 4.0 g/l Glucose 4.0 g/l CaCO3 2.0 g/l Distilled water
12083	N-Z-AMINE-MEDIUM (DSMZ Medium 554)		Medium recipe at MediaDive	Name: N-Z-AMINE-MEDIUM (DSMZ Medium 554) Composition: Starch 20.0 g/l Agar 20.0 g/l Glucose 10.0 g/l N-Z amine 5.0 g/l Yeast extract 5.0 g/l CaCO3 1.0 g/l Distilled water
12083	ROLLED OATS MINERAL MEDIUM (DSMZ Medium 84)		Medium recipe at MediaDive	Name: ROLLED OATS MINERAL MEDIUM (DSMZ Medium 84) Composition: Agar 20.0 g/l Rolled oats 20.0 g/l ZnSO4 x 7 H2O 0.001 g/l MnCl2 x 4 H2O 0.001 g/l FeSO4 x 7 H2O 0.001 g/l Distilled water
12083	GPHF-MEDIUM (DSMZ Medium 553)		Medium recipe at MediaDive	Name: GPHF-MEDIUM (DSMZ Medium 553) Composition: Agar 20.0 g/l Glucose 10.0 g/l Beef extract 5.0 g/l Yeast extract 5.0 g/l Casein peptone 5.0 g/l CaCl2 x 2 H2O 0.74 g/l Distilled water

Culture temp

Oxygen tolerance

Spore formation

Observation

Pathway annotation

Computationally determined by

@ref	pathway	enzyme coverage	annotated reactions	external links
66794	lipoate biosynthesis	100	5 of 5
66794	methylglyoxal degradation	100	5 of 5
66794	ribulose monophosphate pathway	100	2 of 2
66794	ethanol fermentation	100	2 of 2
66794	phenylacetate degradation (aerobic)	100	5 of 5
66794	cis-vaccenate biosynthesis	100	2 of 2
66794	cardiolipin biosynthesis	100	7 of 7
66794	adipate degradation	100	2 of 2
66794	aspartate and asparagine metabolism	100	9 of 9
66794	acetate fermentation	100	4 of 4
66794	palmitate biosynthesis	100	22 of 22
66794	L-lactaldehyde degradation	100	3 of 3
66794	biotin biosynthesis	100	4 of 4
66794	formaldehyde oxidation	100	3 of 3
66794	C4 and CAM-carbon fixation	100	8 of 8
66794	gluconeogenesis	100	8 of 8
66794	anapleurotic synthesis of oxalacetate	100	1 of 1
66794	tetrahydrofolate metabolism	100	14 of 14
66794	folate polyglutamylation	100	1 of 1
66794	taurine degradation	100	1 of 1
66794	ceramide biosynthesis	100	1 of 1
66794	coenzyme A metabolism	100	4 of 4
66794	suberin monomers biosynthesis	100	2 of 2
66794	UDP-GlcNAc biosynthesis	100	3 of 3
66794	CDP-diacylglycerol biosynthesis	100	2 of 2
66794	pentose phosphate pathway	90.91	10 of 11
66794	valine metabolism	88.89	8 of 9
66794	molybdenum cofactor biosynthesis	88.89	8 of 9
66794	chorismate metabolism	88.89	8 of 9
66794	propanol degradation	85.71	6 of 7
66794	reductive acetyl coenzyme A pathway	85.71	6 of 7
66794	glutamate and glutamine metabolism	85.71	24 of 28
66794	ubiquinone biosynthesis	85.71	6 of 7
66794	heme metabolism	85.71	12 of 14
66794	methionine metabolism	84.62	22 of 26
66794	phenylalanine metabolism	84.62	11 of 13
66794	vitamin B1 metabolism	84.62	11 of 13
66794	degradation of sugar alcohols	81.25	13 of 16
66794	propionate fermentation	80	8 of 10
66794	Entner Doudoroff pathway	80	8 of 10
66794	flavin biosynthesis	80	12 of 15
66794	peptidoglycan biosynthesis	80	12 of 15
66794	glycogen metabolism	80	4 of 5
66794	vitamin B12 metabolism	79.41	27 of 34
66794	purine metabolism	78.72	74 of 94
66794	photosynthesis	78.57	11 of 14
66794	citric acid cycle	78.57	11 of 14
66794	CO2 fixation in Crenarchaeota	77.78	7 of 9
66794	serine metabolism	77.78	7 of 9
66794	NAD metabolism	77.78	14 of 18
66794	leucine metabolism	76.92	10 of 13
66794	glycolysis	76.47	13 of 17
66794	CMP-KDO biosynthesis	75	3 of 4
66794	isoleucine metabolism	75	6 of 8
66794	ppGpp biosynthesis	75	3 of 4
66794	butanoate fermentation	75	3 of 4
66794	degradation of aromatic, nitrogen containing compounds	75	9 of 12
66794	threonine metabolism	70	7 of 10
66794	urea cycle	69.23	9 of 13
66794	histidine metabolism	68.97	20 of 29
66794	alanine metabolism	68.97	20 of 29
66794	acetoin degradation	66.67	2 of 3
66794	glycolate and glyoxylate degradation	66.67	4 of 6
66794	cysteine metabolism	66.67	12 of 18
66794	acetyl CoA biosynthesis	66.67	2 of 3
66794	octane oxidation	66.67	2 of 3
66794	IAA biosynthesis	66.67	2 of 3
66794	tryptophan metabolism	65.79	25 of 38
66794	phenol degradation	65	13 of 20
66794	oxidative phosphorylation	64.84	59 of 91
66794	tyrosine metabolism	64.29	9 of 14
66794	glutathione metabolism	64.29	9 of 14
66794	proline metabolism	63.64	7 of 11
66794	6-hydroxymethyl-dihydropterin diphosphate biosynthesis	62.5	5 of 8
66794	ketogluconate metabolism	62.5	5 of 8
66794	non-pathway related	60.53	23 of 38
66794	gallate degradation	60	3 of 5
66794	glycine betaine biosynthesis	60	3 of 5
66794	myo-inositol biosynthesis	60	6 of 10
66794	pyrimidine metabolism	60	27 of 45
66794	4-hydroxyphenylacetate degradation	60	6 of 10
66794	factor 420 biosynthesis	60	3 of 5
66794	metabolism of amino sugars and derivatives	60	3 of 5
66794	arachidonate biosynthesis	60	3 of 5
66794	starch degradation	60	6 of 10
66794	lysine metabolism	59.52	25 of 42
66794	arginine metabolism	58.33	14 of 24
66794	lipid metabolism	58.06	18 of 31
66794	isoprenoid biosynthesis	57.69	15 of 26
66794	dolichol and dolichyl phosphate biosynthesis	50	1 of 2
66794	kanosamine biosynthesis II	50	1 of 2
66794	coenzyme M biosynthesis	50	5 of 10
66794	pantothenate biosynthesis	50	3 of 6
66794	mannosylglycerate biosynthesis	50	1 of 2
66794	aminopropanol phosphate biosynthesis	50	1 of 2
66794	phenylmercury acetate degradation	50	1 of 2
66794	toluene degradation	50	2 of 4
66794	sulfopterin metabolism	50	2 of 4
66794	quinate degradation	50	1 of 2
66794	glycine metabolism	50	5 of 10
66794	glycogen biosynthesis	50	2 of 4
66794	degradation of hexoses	50	9 of 18
66794	1,4-dihydroxy-6-naphthoate biosynthesis	50	3 of 6
66794	selenocysteine biosynthesis	50	3 of 6
66794	degradation of sugar acids	48	12 of 25
66794	polyamine pathway	47.83	11 of 23
66794	bile acid biosynthesis, neutral pathway	47.06	8 of 17
66794	degradation of pentoses	46.43	13 of 28
66794	phenylpropanoid biosynthesis	46.15	6 of 13
66794	vitamin B6 metabolism	45.45	5 of 11
66794	d-mannose degradation	44.44	4 of 9
66794	arachidonic acid metabolism	44.44	8 of 18
66794	nitrate assimilation	44.44	4 of 9
66794	lipid A biosynthesis	44.44	4 of 9
66794	androgen and estrogen metabolism	43.75	7 of 16
66794	bacilysin biosynthesis	40	2 of 5
66794	vitamin K metabolism	40	2 of 5
66794	sulfate reduction	38.46	5 of 13
66794	dTDPLrhamnose biosynthesis	37.5	3 of 8
66794	carnitine metabolism	37.5	3 of 8
66794	d-xylose degradation	36.36	4 of 11
66794	cyanate degradation	33.33	1 of 3
66794	3-phenylpropionate degradation	33.33	5 of 15
66794	(5R)-carbapenem carboxylate biosynthesis	33.33	1 of 3
66794	benzoyl-CoA degradation	28.57	2 of 7
66794	cholesterol biosynthesis	27.27	3 of 11
66794	metabolism of disaccharids	27.27	3 of 11
66794	lactate fermentation	25	1 of 4
66794	cyclohexanol degradation	25	1 of 4
66794	catecholamine biosynthesis	25	1 of 4
66794	phosphatidylethanolamine bioynthesis	23.08	3 of 13
66794	ascorbate metabolism	22.73	5 of 22
66794	4-hydroxymandelate degradation	22.22	2 of 9

Isolation source categories

Isolation

Taxonmaps

69479

Global distribution of 16S sequence AF138733 (>99% sequence identity) for Thermoflavimicrobium dichotomicum subclade from Microbeatlas

Aquatic Samples	386
Soil Samples	1339
Animal Samples	207
Plant Samples	136
Total Samples	2068

Risk assessment

Genome sequences

@ref	Description	Assembly level	INSDC accession	BV-BRC accession	IMG accession	NCBI tax ID	Score
67770	IMG-taxon 2675903228 annotated assembly for Thermoflavimicrobium dichotomicum DSM 44778	contig	GCA_900114055	46223.5	2675903228	46223	52.07

16S sequences

@ref	Description	Accession	Length	Database	NCBI tax ID
20218	Thermoactinomyces dichotomicus KCTC 3667 16S ribosomal RNA gene, complete sequence	AF138733	1475		46223
20218	Thermoactinomyces dichotomicus 16S ribosomal RNA (16S rRNA) sequence	L16902	1463		46223

GC content

Predictions

Literature

Topic	Title	Authors	Journal	DOI	Year
Metabolism	Arsinothricin, an arsenic-containing non-proteinogenic amino acid analog of glutamate, is a broad-spectrum antibiotic.	Nadar VS, Chen J, Dheeman DS, Galvan AE, Yoshinaga-Sakurai K, Kandavelu P, Sankaran B, Kuramata M, Ishikawa S, Rosen BP, Yoshinaga M, Yoshinaga M.	Commun Biol	10.1038/s42003-019-0365-y	2019
	A highly thermostable ethyl carbamate-degrading urethanase from Thermoflavimicrobium dichotomicum.	Liu Q, Wang H, Li X, Tian S, Wu C, Chen Y, Qian S, Zhao S, Zhang W, Cheng F, Yang G, Wang T.	Int J Biol Macromol	10.1016/j.ijbiomac.2025.142245	2025
Metabolism	Production of l-ribose from l-arabinose by co-expression of l-arabinose isomerase and d-lyxose isomerase in Escherichia coli.	Wu H, Huang J, Deng Y, Zhang W, Mu W.	Enzyme Microb Technol	10.1016/j.enzmictec.2019.109443	2020
	Engineering the thermostability of d-lyxose isomerase from Caldanaerobius polysaccharolyticus via multiple computer-aided rational design for efficient synthesis of d-mannose.	Wu H, Yi M, Wu X, Ding Y, Pu M, Wen L, Cheng Y, Zhang W, Mu W.	Synth Syst Biotechnol	10.1016/j.synbio.2023.04.003	2023
	Recent advances in biotransformation, extraction and green production of D-mannose.	Wang P, Zheng Y, Li Y, Shen J, Dan M, Wang D.	Curr Res Food Sci	10.1016/j.crfs.2021.12.002	2022
	Conversion of L-arabinose to L-ribose by genetically engineered Candida tropicalis.	Yeo IS, Cho BK, Kim JH.	Bioprocess Biosyst Eng	10.1007/s00449-020-02506-2	2021
Enzymology	Alkalithermophilic actinomycetes in a subtropical area of Jujuy, Argentina.	Carrillo L, Benitez Ahrendts MR, Maldonado MJ.	Rev Argent Microbiol		2009
	Characterization of a D-lyxose isomerase from Bacillus velezensis and its application for the production of D-mannose and L-ribose.	Guo Z, Long L, Ding S.	AMB Express	10.1186/s13568-019-0877-3	2019
Phylogeny	Proposal of the genus Thermoactinomyces sensu stricto and three new genera, Laceyella, Thermoflavimicrobium and Seinonella, on the basis of phenotypic, phylogenetic and chemotaxonomic analyses.	Yoon JH, Kim IG, Shin YK, Park YH	Int J Syst Evol Microbiol	10.1099/ijs.0.63203-0	2005
Phylogeny	Thermoflavimicrobium daqui sp. nov., a thermophilic microbe isolated from Moutai-flavour Daqu.	Li DN, Huang W, Qiu SY	Int J Syst Evol Microbiol	10.1099/ijsem.0.003528	2019