Azospirillum thermophilum (KCTC 62259, CFH 70021, CCTCC AB 2018121)

Name: Azospirillum thermophilum (KCTC 62259, CFH 70021, CCTCC AB 2018121)
Creator: BacDive
License: http://creativecommons.org/licenses/by/4.0/

BacDive ID 167820

Type strain

Culture col. no. KCTC 62259 CFH 70021 CCTCC AB 2018121

NCBI tax ID(s) 2202148

Special Collections Literature strains KCTC

Links

Azospirillum thermophilum KCTC 62259 is an aerobe, Gram-negative, motile bacterium that forms circular colonies and was isolated from Hot spring soil sample.

Gram-negative motile rod-shaped colony-forming aerobe genome sequence 16S sequence Bacteria

Name and taxonomic classification

SI-ID 285864

KCTC 62259

@ref 20215

Last LPSN update 2026-05-29 08:46:14

Domain Bacteria

Phylum Pseudomonadota

Class Alphaproteobacteria

Order Rhodospirillales

Family Azospirillaceae

Genus Azospirillum

Species Azospirillum thermophilum

Full scientific name Azospirillum thermophilum Zhao et al. 2020

Morphology

Cell morphology

@ref	Gram stain	Cell length	Cell width	Cell shape	Motility	Confidence
69521	negative	1.0-3.0 µm	0.4-0.7 µm	rod-shaped
125439	negative					98.156

Colony morphology

@ref	Colony color	Colony shape	Incubation period	Medium used
69521	white	circular	3 days	T5 agar

Culture and growth conditions

Culture medium

@ref	Name	Growth
69521	T5 agar
69521	T5

Culture temp

@ref	Growth	Type	Temperature (°C)
69521	positive	growth	15.0-50.0
69521	positive	optimum	50

Culture pH

@ref	Ability	Type	PH
69521	positive	optimum	7
69521	positive	growth	5.0-7.0

Physiology and metabolism

Oxygen tolerance

@ref	Oxygen tolerance	Confidence
69521	aerobe
125439	obligate aerobe	94.658

Spore formation

@ref	Spore formation	Confidence
125439		99.613

Halophily

@ref	Salt	Growth	Tested relation	Concentration
69521	NaCl	positive	growth	0.0-3.0 %(w/v)
69521	NaCl	positive	optimum	0 %(w/v)

Observation

@ref	Observation
69521	Positive for milk coagulation and milk peptonization.
69521	The polar lipid profile consists of diphosphatidylglycerol, phosphatidylglycerol, phosphatidylcholine, phosphatidylmehtylethanolamine, two aminolipids and an unidentified phospholipid.

Metabolite utilization

@ref	Chebi-ID	Metabolite	Utilization activity	Kind of utilization tested
69521	16651 ChEBI	(S)-lactate	+	carbon source
69521	37054 ChEBI	3-hydroxybutyrate	+	carbon source
69521	58143 ChEBI	5-dehydro-D-gluconate	+	builds acid from
69521	30089 ChEBI	acetate	+	carbon source
69521	17925 ChEBI	alpha-D-glucose	+	carbon source
69521	27613 ChEBI	amygdalin	+	builds acid from
69521	18305 ChEBI	arbutin	+	builds acid from
69521	29016 ChEBI	arginine	-	hydrolysis
69521	73706 ChEBI	bromosuccinate	+	carbon source
69521	17057 ChEBI	cellobiose	+	builds acid from
69521	15824 ChEBI	D-fructose	+	carbon source
69521	28847 ChEBI	D-fucose	+	builds acid from
69521	28847 ChEBI	D-fucose	+	carbon source
69521	18024 ChEBI	D-galacturonic acid	+	carbon source
69521	18391 ChEBI	D-gluconate	+	carbon source
69521	15748 ChEBI	D-glucuronate	+	carbon source
69521	62318 ChEBI	D-lyxose	+	builds acid from
69521	16899 ChEBI	D-mannitol	+	carbon source
69521	17924 ChEBI	D-sorbitol	+	carbon source
69521	16443 ChEBI	D-tagatose	+	builds acid from
69521	16551 ChEBI	D-trehalose	+	carbon source
69521	65327 ChEBI	D-xylose	+	builds acid from
69521	4853 ChEBI	esculin	+	builds acid from
69521	4853 ChEBI	esculin	+	hydrolysis
69521	28260 ChEBI	galactose	+	builds acid from
69521	16865 ChEBI	gamma-aminobutyric acid	+	carbon source
69521	5291 ChEBI	gelatin	-	hydrolysis
69521	24265 ChEBI	gluconate	+	builds acid from
69521	32323 ChEBI	glucuronamide	+	carbon source
69521	17754 ChEBI	glycerol	+	builds acid from
69521	15428 ChEBI	glycine	+	nitrogen source
69521	17368 ChEBI	hypoxanthine	+	nitrogen source
69521	16977 ChEBI	L-alanine	+	nitrogen source
69521	16467 ChEBI	L-arginine	+	nitrogen source
69521	17196 ChEBI	L-asparagine	+	nitrogen source
69521	18287 ChEBI	L-fucose	+	builds acid from
69521	17464 ChEBI	L-galactonic acid gamma-lactone	+	carbon source
69521	29985 ChEBI	L-glutamate	+	nitrogen source
69521	18019 ChEBI	L-lysine	+	nitrogen source
69521	15589 ChEBI	L-malate	+	carbon source
69521	17295 ChEBI	L-phenylalanine	+	nitrogen source
69521	17203 ChEBI	L-proline	+	nitrogen source
69521	16414 ChEBI	L-valine	+	nitrogen source
69521	17716 ChEBI	lactose	+	builds acid from
69521	17306 ChEBI	maltose	+	builds acid from
69521	28053 ChEBI	melibiose	+	builds acid from
69521	320055 ChEBI	methyl beta-D-glucopyranoside	+	carbon source
69521	17268 ChEBI	myo-inositol	+	carbon source
69521	63154 ChEBI	N-acetyl-beta-D-mannosamine	+	carbon source
69521	506227 ChEBI	N-acetyl-D-glucosamine	+	carbon source
69521	506227 ChEBI	N-acetylglucosamine	+	builds acid from
69521	17632 ChEBI	nitrate	+	reduction
69521	17272 ChEBI	propionate	+	carbon source
69521	33942 ChEBI	ribose	+	builds acid from
69521	17814 ChEBI	salicin	+	builds acid from
69521	17814 ChEBI	salicin	+	carbon source
69521	28017 ChEBI	starch	-	hydrolysis
69521	17992 ChEBI	sucrose	+	builds acid from
69521	27082 ChEBI	trehalose	+	builds acid from
69521	27897 ChEBI	tryptophan	+	nitrogen source
69521	32528 ChEBI	turanose	+	builds acid from
69521	32528 ChEBI	turanose	+	carbon source
69521	53424 ChEBI	tween 20	+	degradation
69521	53423 ChEBI	tween 40	-	degradation
69521	53425 ChEBI	tween 60	-	degradation
69521	53426 ChEBI	tween 80	-	degradation

Metabolite production

@ref	Chebi-ID	Metabolite	Production
69521	16136 ChEBI	hydrogen sulfide
69521	35581 ChEBI	indole

Enzymes

@ref	Value	Activity	Ec
69521	catalase	+	1.11.1.6
69521	cytochrome oxidase	+	1.9.3.1
69521	urease	+	3.5.1.5

Fatty acid profile

Metadata FA analysis

@ref

69521

Fatty acid	Percentage
C18:1 w7c / C18:1 w6c	40.9
C16:0	27.6
C19:0 cyclo w8c	11.4

Isolation, sampling and environmental information

Isolation

@ref	Sample type	Geographic location	Country	Country ISO 3 Code	Continent	Enrichment culture	Enrichment culture duration	Enrichment culture temperature
69521	Hot spring soil sample	Yunnan province	China	CHN	Asia	T5	14 days	50

Interaction and safety

Sequence information

Genome sequences

@ref	Description	Assembly level	INSDC accession	BV-BRC accession	IMG accession	NCBI tax ID	Score
69521	ASM313079v1 assembly for Azospirillum thermophilum CFH 70021	complete	GCA_003130795	528244.16	2848865092	2202148	90.47

16S sequences

@ref	Description	Accession	Length	Database	NCBI tax ID
20215	Azospirillum thermophilum 16S ribosomal RNA gene, partial sequence	MH265951	1509		2202148

GC content

@ref	GC-content (mol%)	Method
69521	69.3	genome sequence analysis

Genome-based predictions

Predictions

Predictions from Deep Learning for Genome Sequence Data. R package version 0.3.1 based on: ASM313079v1 assembly for Azospirillum thermophilum CFH 70021
NCBI: GCA_003130795

@ref	Trait	Model	Prediction	Confidence in %	In training data
125439	motility	BacteriaNetⓘ	yes	62.76	no
125439	gram_stain	BacteriaNetⓘ	negative	98.16	no
125439	oxygen_tolerance	BacteriaNetⓘ	obligate aerobe	94.66	no
125439	spore_formation	BacteriaNetⓘ	no	99.61	no

Literature

Topic	Title	Authors	Journal	DOI	Year
	Bioinformatics and functional selection of GH77 4-alpha-glucanotransferases for potato starch modification.	Christensen SJ, Madsen MS, Zinck SS, Hedberg C, Sorensen OB, Svensson B, Meyer AS.	N Biotechnol	10.1016/j.nbt.2023.12.002	2024
Phylogeny	Azospirillum thermophilum sp. nov., isolated from a hot spring.	Zhao ZL, Ming H, Ding CL, Ji WL, Cheng LJ, Niu MM, Zhang YM, Zhang LY, Meng XL, Nie GX	Int J Syst Evol Microbiol	10.1099/ijsem.0.003788	2020

References

#20215	Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )
#69521	Zhuo-li Zhao, Hong Ming, Chen-Long Ding, Wei-Li Ji, Li-Jiao Cheng, Ming-ming Niu, Yan-min Zhang, Ling-Yu Zhang, Xiao-Lin Meng, Guo-Xing Nie: Azospirillum thermophilum sp. nov., isolated from a hot spring. IJSEM 70: 2020 ( DOI 10.1099/ijsem.0.003788 )
#125439	Philipp Münch, René Mreches, Martin Binder, Hüseyin Anil Gündüz, Xiao-Yin To, Alice McHardy: deepG: Deep Learning for Genome Sequence Data. R package version 0.3.1 .
#126262	A. Lissin, I. Schober, J. F. Witte, H. Lüken, A. Podstawka, J. Koblitz, B. Bunk, P. Dawyndt, P. Vandamme, P. de Vos, J. Overmann, L. C. Reimer: StrainInfo—the central database for linked microbial strain identifiers. ( DOI 10.1093/database/baaf059 )

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Azospirillum thermophilum (KCTC 62259, CFH 70021, CCTCC AB 2018121)

Name and taxonomic classification

Morphology

Cell morphology

Colony morphology

Culture and growth conditions

Culture medium

Culture temp

Culture pH

Physiology and metabolism

Oxygen tolerance

Spore formation

Halophily

Observation

Metabolite utilization

Metabolite production

Enzymes

Fatty acid profile

Isolation, sampling and environmental information

Isolation

Interaction and safety

Sequence information

Genome sequences

Genome browser: GCA_003130795

16S sequences

GC content

Genome-based predictions

Predictions

Literature

Literature

References

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