Name: Azospirillum thermophilum KCTC 62259
Creator: BacDive
License: https://creativecommons.org/licenses/by/4.0/

Strain identifier

BacDive ID: 167820

Type strain:

Species: Azospirillum thermophilum

Culture col. no.: KCTC 62259, CFH 70021, CCTCC AB 2018121

NCBI tax ID(s): 2202148 (species)

Special Collections

Section

Azospirillum thermophilum KCTC 62259 is an aerobe, Gram-negative, motile bacterium that forms circular colonies and was isolated from Hot spring soil sample.

colony-forming
Gram-negative
motile
rod-shaped
aerobe
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	09-12-2024 (DD-MM-YYYY)
	Domain	*Bacteria*
	Phylum	*Pseudomonadota*
	Class	*Alphaproteobacteria*
	Order	*Rhodospirillales*
	Family	*Azospirillaceae*
	Genus	*Azospirillum*
	Species	**Azospirillum thermophilum**
	Full Scientific Name (LPSN)	Azospirillum thermophilum Zhao et al. 2020

Information on morphological and physiological properties Morphology

[Ref.: #69521]	Gram stain	negative

[Ref.: #69521]	Cell length	1.0-3.0 µm

[Ref.: #69521]	Cell width	0.4-0.7 µm

[Ref.: #69521]	Cell shape	rod-shaped

[Ref.: #69521]	Motility	yes

[Ref.: #69521]	Colony color	white
[Ref.: #69521]	Colony shape	circular
[Ref.: #69521]	Incubation period	3 days
[Ref.: #69521]	Cultivation medium used	T5 agar

Information on culture and growth conditions Culture and growth conditions

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Culture medium

T5 agar

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Culture medium growth

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Culture medium

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Culture medium growth

	Temperatures	Kind of temperature		Temperature
[Ref.: #69521]			growth	15.0-50.0 °C
[Ref.: #69521]			optimum	50 °C

	pH	Kind of pH		pH
[Ref.: #69521]			optimum	7
[Ref.: #69521]			growth	5.0-7.0

Information on physiology and metabolism Physiology and metabolism

[Ref.: #69521]

Oxygen tolerance

aerobe

	Halophily	Salt	Tested relation		Salt conc.
[Ref.: #69521]		NaCl		growth	0.0-3.0	%(w/v)
[Ref.: #69521]		NaCl		optimum	0	%(w/v)

[Ref.: #69521]	Observation	Positive for milk coagulation and milk peptonization.
[Ref.: #69521]	Observation	The polar lipid profile consists of diphosphatidylglycerol, phosphatidylglycerol, phosphatidylcholine, phosphatidylmehtylethanolamine, two aminolipids and an unidentified phospholipid.

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #69521]	(S)-lactate ChEBI	+	carbon source
[Ref.: #69521]	3-hydroxybutyrate ChEBI	+	carbon source
[Ref.: #69521]	5-dehydro-D-gluconate ChEBI	+	builds acid from
[Ref.: #69521]	acetate ChEBI	+	carbon source
[Ref.: #69521]	alpha-D-glucose ChEBI	+	carbon source
[Ref.: #69521]	amygdalin ChEBI	+	builds acid from
[Ref.: #69521]	arbutin ChEBI	+	builds acid from
[Ref.: #69521]	arginine ChEBI	-	hydrolysis
[Ref.: #69521]	bromosuccinate ChEBI	+	carbon source
[Ref.: #69521]	cellobiose ChEBI	+	builds acid from
[Ref.: #69521]	D-fructose ChEBI	+	carbon source
[Ref.: #69521]	D-fucose ChEBI	+	builds acid from
[Ref.: #69521]	D-fucose ChEBI	+	carbon source
[Ref.: #69521]	D-galacturonic acid ChEBI	+	carbon source
[Ref.: #69521]	D-gluconate ChEBI	+	carbon source
[Ref.: #69521]	D-glucuronate ChEBI	+	carbon source
[Ref.: #69521]	D-lyxose ChEBI	+	builds acid from
[Ref.: #69521]	D-mannitol ChEBI	+	carbon source
[Ref.: #69521]	D-salicin ChEBI	+	carbon source
[Ref.: #69521]	D-sorbitol ChEBI	+	carbon source
[Ref.: #69521]	D-tagatose ChEBI	+	builds acid from
[Ref.: #69521]	D-trehalose ChEBI	+	carbon source
[Ref.: #69521]	D-xylose ChEBI	+	builds acid from
[Ref.: #69521]	esculin ChEBI	+	builds acid from
[Ref.: #69521]	esculin ChEBI	+	hydrolysis
[Ref.: #69521]	galactose ChEBI	+	builds acid from
[Ref.: #69521]	gamma-aminobutyric acid ChEBI	+	carbon source
[Ref.: #69521]	gelatin ChEBI	-	hydrolysis
[Ref.: #69521]	gluconate ChEBI	+	builds acid from
[Ref.: #69521]	glucuronamide ChEBI	+	carbon source
[Ref.: #69521]	glycerol ChEBI	+	builds acid from
[Ref.: #69521]	glycine ChEBI	+	nitrogen source
[Ref.: #69521]	hypoxanthine ChEBI	+	nitrogen source
[Ref.: #69521]	L-alanine ChEBI	+	nitrogen source
[Ref.: #69521]	L-arginine ChEBI	+	nitrogen source
[Ref.: #69521]	L-asparagine ChEBI	+	nitrogen source
[Ref.: #69521]	L-fucose ChEBI	+	builds acid from
[Ref.: #69521]	L-galactonic acid gamma-lactone ChEBI	+	carbon source
[Ref.: #69521]	L-glutamate ChEBI	+	nitrogen source
[Ref.: #69521]	L-lysine ChEBI	+	nitrogen source
[Ref.: #69521]	L-malate ChEBI	+	carbon source
[Ref.: #69521]	L-phenylalanine ChEBI	+	nitrogen source
[Ref.: #69521]	L-proline ChEBI	+	nitrogen source
[Ref.: #69521]	L-valine ChEBI	+	nitrogen source
[Ref.: #69521]	lactose ChEBI	+	builds acid from
[Ref.: #69521]	maltose ChEBI	+	builds acid from
[Ref.: #69521]	melibiose ChEBI	+	builds acid from
[Ref.: #69521]	methyl beta-D-glucopyranoside ChEBI	+	carbon source
[Ref.: #69521]	myo-inositol ChEBI	+	carbon source
[Ref.: #69521]	N-acetyl-beta-D-mannosamine ChEBI	+	carbon source
[Ref.: #69521]	N-acetyl-D-glucosamine ChEBI	+	carbon source
[Ref.: #69521]	N-acetylglucosamine ChEBI	+	builds acid from
[Ref.: #69521]	nitrate ChEBI	+	reduction
[Ref.: #69521]	propionate ChEBI	+	carbon source
[Ref.: #69521]	ribose ChEBI	+	builds acid from
[Ref.: #69521]	salicin ChEBI	+	builds acid from
[Ref.: #69521]	starch ChEBI	-	hydrolysis
[Ref.: #69521]	sucrose ChEBI	+	builds acid from
[Ref.: #69521]	trehalose ChEBI	+	builds acid from
[Ref.: #69521]	tryptophan ChEBI	+	nitrogen source
[Ref.: #69521]	turanose ChEBI	+	builds acid from
[Ref.: #69521]	turanose ChEBI	+	carbon source
[Ref.: #69521]	tween 20 ChEBI	+	degradation
[Ref.: #69521]	tween 40 ChEBI	-	degradation
[Ref.: #69521]	tween 60 ChEBI	-	degradation
[Ref.: #69521]	tween 80 ChEBI	-	degradation
	Only first 10 entries are displayed. Click here to see all.

	Metabolite production	Metabolite	Production
[Ref.: #69521]		hydrogen sulfide ChEBI	no
[Ref.: #69521]		indole ChEBI	no

	Enzyme	Enzyme activity	EC number
[Ref.: #69521]	catalase	+	1.11.1.6
[Ref.: #69521]	cytochrome oxidase	+	1.9.3.1
[Ref.: #69521]	urease	+	3.5.1.5

Fatty acid profile

Metadata FA analysis

Reference

[Ref.: #69521]

Fatty acid	Percentage
C18:1 w7c / C18:1 w6c	40.9
C16:0	27.6
C19:0 cyclo w8c	11.4

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #69521]	Sample type/isolated from	Hot spring soil sample
[Ref.: #69521]	Geographic location (country and/or sea, region)	Yunnan province
[Ref.: #69521]	Country	China
[Ref.: #69521]	Country ISO 3 Code	CHN
[Ref.: #69521]	Continent	Asia
[Ref.: #69521]	Enrichment culture	T5
[Ref.: #69521]	Enrichment culture duration	14 days
[Ref.: #69521]	Enrichment culture temperature	50 ̊C
* marker position based on {}

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #20215]	Azospirillum thermophilum 16S ribosomal RNA gene, partial sequence	MH265951	1509	GenBank ENA	2202148 tax ID

Genome sequence information:

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #69521]	Azospirillum thermophilum CFH 70021	GCA_003130795	complete	GenBank ENA	2202148 tax ID
[Ref.: #69521]	genome sequence	CP029352		GenBank ENA	2202148 tax ID
[Ref.: #69521]	genome sequence	CP029353		GenBank ENA	2202148 tax ID
[Ref.: #69521]	genome sequence	CP029354		GenBank ENA	2202148 tax ID
[Ref.: #69521]	genome sequence	CP029355		GenBank ENA	2202148 tax ID

[Ref.: #69521]

GC-content

69.3 mol%

genome sequence analysis

Availability in culture collections External links

[Ref.: #20215]

Culture collection no.

KCTC 62259, CFH 70021, CCTCC AB 2018121

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Azospirillum thermophilum sp. nov., isolated from a hot spring.	Zhao ZL, Ming H, Ding CL, Ji WL, Cheng LJ, Niu MM, Zhang YM, Zhang LY, Meng XL, Nie GX	Int J Syst Evol Microbiol	10.1099/ijsem.0.003788	2020	*

References

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#69521

Zhuo-li Zhao, Hong Ming, Chen-Long Ding, Wei-Li Ji, Li-Jiao Cheng, Ming-ming Niu, Yan-min Zhang, Ling-Yu Zhang, Xiao-Lin Meng, Guo-Xing Nie: Azospirillum thermophilum sp. nov., isolated from a hot spring. IJSEM 70: 2020 ( DOI 10.1099/ijsem.0.003788 )

* These data were automatically processed and therefore are not curated

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