Thermithiobacillus tepidarius (DSM 3134, ATCC 43215)

Name: Thermithiobacillus tepidarius (DSM 3134, ATCC 43215)
Creator: BacDive
License: http://creativecommons.org/licenses/by/4.0/

BacDive ID 16736

Type strain

Culture col. no. DSM 3134 ATCC 43215

NCBI tax ID(s) 1123368 929

Special Collections DSMZ

History DSMZ <- D. P. Kelly <- A. P. Wood, Univ. Warwick, Coventry, U.K. {1983}

Links

Thermithiobacillus tepidarius DSM 3134 is a bacterium that was isolated from thermal sulfur spring.

genome sequence 16S sequence Bacteria

Name and taxonomic classification

SI-ID 93015

ATCC 43215,DSM 3134

@ref 20215

Last LPSN update 2026-05-29 08:28:51

Domain Bacteria

Phylum Pseudomonadota

Class Acidithiobacillia

Order Acidithiobacillales

Family Thermithiobacillaceae

Genus Thermithiobacillus

Species Thermithiobacillus tepidarius

Full scientific name Thermithiobacillus tepidarius (Wood and Kelly 1985) Kelly and Wood 2000

Synonyms (1)

Thiobacillus tepidarius

Morphology

Cell morphology

@ref	Gram stain	Confidence
125438	negative	97.828
125439	negative	91.594

Culture and growth conditions

Culture medium

@ref	Name	Growth	Medium link	Composition
1309	HALOTHIOBACILLUS NEAPOLITANUS MEDIUM (DSMZ Medium 68)		Medium recipe at MediaDive	Name: HALOTHIOBACILLUS NEAPOLITANUS MEDIUM (DSMZ Medium 68) Composition: Agar 15.0 g/l Na2S2O3 x 5 H2O 10.0 g/l KH2PO4 4.0 g/l K2HPO4 4.0 g/l MgSO4 x 7 H2O 0.8 g/l NH4Cl 0.4 g/l Na2-EDTA 0.25 g/l ZnSO4 x 7 H2O 0.11 g/l CaCl2 x 2 H2O 0.0277 g/l MnCl2 x 4 H2O 0.0253 g/l FeSO4 x 7 H2O 0.025 g/l CoCl2 x 6 H2O 0.00805 g/l CuSO4 x 5 H2O 0.00785 g/l (NH4)6Mo7O24 x 4 H2O 0.0055 g/l Bromocresol purple Distilled water

Culture temp

@ref	Growth	Type	Temperature (°C)
1309	positive	growth	45

Physiology and metabolism

Spore formation

@ref	Spore formation	Confidence
125439		99.475

Isolation, sampling and environmental information

Isolation source categories

Cat1	Cat2	Cat3
#Environmental	#Aquatic	#Thermal spring
#Condition	#Sulfuric	-

Isolation

@ref	Sample type	Geographic location	Country	Country ISO 3 Code	Continent
1309	thermal sulfur spring	Great Roman Bath at Bath	United Kingdom	GBR	Europe

Taxonmaps

69479

Global distribution of 16S sequence AJ459801 (>99% sequence identity) for Thermithiobacillus tepidarius subclade from Microbeatlas

Aquatic Samples	388
Soil Samples	178
Animal Samples	117
Plant Samples	25
Total Samples	708

Interaction and safety

Risk assessment

@ref	Biosafety level	Biosafety level comment
1309	1	Risk group (German classification) According to TRBA 466

Sequence information

Genome sequences

@ref	Description	Assembly level	INSDC accession	BV-BRC accession	IMG accession	NCBI tax ID	Score
124043	TTP_v4 assembly for Thermithiobacillus tepidarius DSM 3134	complete	GCA_048300925			1123368	98.52
66792	ASM42382v1 assembly for Thermithiobacillus tepidarius DSM 3134	scaffold	GCA_000423825	1123368.4	2523533554	1123368	69.7

16S sequences

@ref	Description	Accession	Length	Database	NCBI tax ID
20218	Thermithiobacillus tepidarius 16S ribosomal RNA (16S rRNA), complete sequence	AH001786	708		929
1309	Thermithiobacillus tepidarius 16S rRNA gene, strain DSM 3134	AJ459801	1475		929

GC content

@ref	GC-content (mol%)	Method
1309	66.8	sequence analysis

Genome-based predictions

Predictions

Predictions from Deep Learning for Genome Sequence Data. R package version 0.3.1 based on: TTP_v4 assembly for Thermithiobacillus tepidarius DSM 3134
NCBI: GCA_048300925

@ref	Trait	Model	Prediction	Confidence in %	In training data
125439	oxygen_tolerance	BacteriaNetⓘ	obligate aerobe	83.79	no
125439	gram_stain	BacteriaNetⓘ	negative	91.59	no
125439	motility	BacteriaNetⓘ	yes	63.82	no
125439	spore_formation	BacteriaNetⓘ	no	99.48	no

Predictions from bacterial phenotypic traits through improved machine learning using high-quality, curated datasets based on: Thermithiobacillus tepidarius DSM 3134 DSM 3134
NCBI: GCA_000423825.1

@ref	Trait	Model	Prediction	Confidence in %	In training data
125438	gram-positive	gram-positiveⓘ	no	97.83	no
125438	anaerobic	anaerobicⓘ	no	68.29	no
125438	aerobic	aerobicⓘ	yes	56.87	no
125438	spore-forming	spore-formingⓘ	no	88.76	no
125438	thermophilic	thermophileⓘ	no	87.08	no
125438	flagellated	motile2+ⓘ	yes	72.27	no

Literature

Topic	Title	Authors	Journal	DOI	Year
	Exploring the eco-evolutionary role of plasmids and defense systems in 'Fervidacidithiobacillus caldus' extreme acidophile.	Pacheco-Acosta S, Castro-Toro G, Rojas-Villalobos C, Valenzuela C, Haristoy JJ, Zapata-Araya A, Moya-Beltran A, Sepulveda-Rebolledo P, Perez-Rueda E, Ulloa R, Giaveno A, Issotta F, Diez B, Beard S, Quatrini R.	Front Microbiol	10.3389/fmicb.2025.1610279	2025
Genetics	Acidithiobacillia class members originating at sites within the Pacific Ring of Fire and other tectonically active locations and description of the novel genus 'Igneacidithiobacillus'.	Arisan D, Moya-Beltran A, Rojas-Villalobos C, Issotta F, Castro M, Ulloa R, Chiacchiarini PA, Diez B, Martin AJM, Nancucheo I, Giaveno A, Johnson DB, Quatrini R.	Front Microbiol	10.3389/fmicb.2024.1360268	2024
Genetics	Integrative Genomics Sheds Light on Evolutionary Forces Shaping the Acidithiobacillia Class Acidophilic Lifestyle.	Gonzalez-Rosales C, Vergara E, Dopson M, Valdes JH, Holmes DS.	Front Microbiol	10.3389/fmicb.2021.822229	2021
	Permanent draft genome of Thiobacillus thioparus DSM 505^T, an obligately chemolithoautotrophic member of the Betaproteobacteria.	Hutt LP, Huntemann M, Clum A, Pillay M, Palaniappan K, Varghese N, Mikhailova N, Stamatis D, Reddy T, Daum C, Shapiro N, Ivanova N, Kyrpides N, Woyke T, Boden R.	Stand Genomic Sci	10.1186/s40793-017-0229-3	2017
	Nucleotide Second Messenger-Based Signaling in Extreme Acidophiles of the Acidithiobacillus Species Complex: Partition Between the Core and Variable Gene Complements.	Moya-Beltran A, Rojas-Villalobos C, Diaz M, Guiliani N, Quatrini R, Castro M.	Front Microbiol	10.3389/fmicb.2019.00381	2019
	The Type IV Secretion System of ICEAfe1: Formation of a Conjugative Pilus in Acidithiobacillus ferrooxidans.	Flores-Rios R, Moya-Beltran A, Pareja-Barrueto C, Arenas-Salinas M, Valenzuela S, Orellana O, Quatrini R.	Front Microbiol	10.3389/fmicb.2019.00030	2019
	Effect of CO₂ Concentration on Uptake and Assimilation of Inorganic Carbon in the Extreme Acidophile Acidithiobacillus ferrooxidans.	Esparza M, Jedlicki E, Gonzalez C, Dopson M, Holmes DS.	Front Microbiol	10.3389/fmicb.2019.00603	2019
	A novel family of integrases associated with prophages and genomic islands integrated within the tRNA-dihydrouridine synthase A (dusA) gene.	Farrugia DN, Elbourne LD, Mabbutt BC, Paulsen IT.	Nucleic Acids Res	10.1093/nar/gkv337	2015
	Advances in Understanding Carboxysome Assembly in Prochlorococcus and Synechococcus Implicate CsoS2 as a Critical Component.	Cai F, Dou Z, Bernstein SL, Leverenz R, Williams EB, Heinhorst S, Shively J, Cannon GC, Kerfeld CA.	Life (Basel)	10.3390/life5021141	2015
	Permanent draft genome of Thermithiobaclillus tepidarius DSM 3134(T), a moderately thermophilic, obligately chemolithoautotrophic member of the Acidithiobacillia.	Boden R, Hutt LP, Huntemann M, Clum A, Pillay M, Palaniappan K, Varghese N, Mikhailova N, Stamatis D, Reddy T, Ngan CY, Daum C, Shapiro N, Markowitz V, Ivanova N, Woyke T, Kyrpides N	Stand Genomic Sci	10.1186/s40793-016-0188-0	2016
	Erratum to: Permanent draft genome of Thermithiobacillus tepidarius DSM 3134(T), a moderately thermophilic, obligately chemolithoautotrophic member of the Acidithiobacillia.	Boden R, Hutt LP, Huntemann M, Clum A, Pillay M, Palaniappan K, Varghese N, Mikhailova N, Stamatis D, Reddy T, Ngan CY, Daum C, Shapiro N, Markowitz V, Ivanova N, Woyke T, Kyrpides N	Stand Genomic Sci	10.1186/s40793-016-0197-z	2016
Phylogeny	Thermithiobacillus plumbiphilus sp. nov., a sulfur-oxidizing bacterium isolated from lead sulfide.	Watanabe T, Miura A, Shinohara A, Kojima H, Fukui M	Int J Syst Evol Microbiol	10.1099/ijsem.0.000972	2016

References

#1309	Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 3134
#20215	Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )
#20218	Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.: StrainInfo introduces electronic passports for microorganisms.. Syst Appl Microbiol. 37: 42 - 50 2014 ( DOI 10.1016/j.syapm.2013.11.002 , PubMed 24321274 )
#66792	Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .
#69479	João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .
#124043	Isabel Schober, Julia Koblitz: Data extracted from sequence databases, automatically matched based on designation and taxonomy .
#125438	Julia Koblitz, Lorenz Christian Reimer, Rüdiger Pukall, Jörg Overmann: Predicting bacterial phenotypic traits through improved machine learning using high-quality, curated datasets. 2024 ( DOI 10.1101/2024.08.12.607695 )
#125439	Philipp Münch, René Mreches, Martin Binder, Hüseyin Anil Gündüz, Xiao-Yin To, Alice McHardy: deepG: Deep Learning for Genome Sequence Data. R package version 0.3.1 .
#126262	A. Lissin, I. Schober, J. F. Witte, H. Lüken, A. Podstawka, J. Koblitz, B. Bunk, P. Dawyndt, P. Vandamme, P. de Vos, J. Overmann, L. C. Reimer: StrainInfo—the central database for linked microbial strain identifiers. ( DOI 10.1093/database/baaf059 )

Rate our new design

Content

About us

Thermithiobacillus tepidarius (DSM 3134, ATCC 43215)

Name and taxonomic classification

Morphology

Cell morphology

Culture and growth conditions

Culture medium

Culture temp

Physiology and metabolism

Spore formation

Isolation, sampling and environmental information

Isolation source categories

Isolation

Taxonmaps

Interaction and safety

Risk assessment

Sequence information

Genome sequences

Genome browser: GCA_048300925

16S sequences

GC content

Genome-based predictions

Predictions

Literature

Literature

References

BacDive

Help

Social Media