Thermus aquaticus (DSM 625, ATCC 25104, YT-1)

Name: Thermus aquaticus (DSM 625, ATCC 25104, YT-1)
Creator: BacDive
License: http://creativecommons.org/licenses/by/4.0/

BacDive ID 16714

Type strain

Strain Designation YT-1

Culture col. no. DSM 625 ATCC 25104 YT-1 NCIMB 11243 NBRC 103206 2 more

NCBI tax ID(s) 271

Special Collections DSMZ JCM

Links

Thermus aquaticus YT-1 is a thermophilic prokaryote that was isolated from hot spring.

thermophilic genome sequence 16S sequence

Name and taxonomic classification

SI-ID 5027

LMG 8924,ATCC 25104,DSM 625,NCIB 11243,NCIMB 11243,IMET 11241,KCTC 2755,JCM 10724,NCAIM B.01703,BCRC 17110,CCRC 17110,BCRC 16071,CCRC 16071,NBRC 103206

@ref 20215

Last LPSN update 2025-12-16 09:50:54

Domain Thermotogati

Phylum Deinococcota

Class Deinococci

Order Thermales

Family Thermaceae

Genus Thermus

Species Thermus aquaticus

Full scientific name Thermus aquaticus Brock and Freeze 1969 (Approved Lists 1980)

BacDive ID	Other strains from **Thermus aquaticus** (1)	Type strain
100091	T. aquaticus STI09686(IMET), 55-A,

Morphology

Culture and growth conditions

Culture medium

@ref	Name	Growth	Medium link	Composition
328	CASTENHOLZ MEDIUM (DSMZ Medium 86)		Medium recipe at MediaDive	Name: CASTENHOLZ MEDIUM (DSMZ Medium 86) Composition: Yeast extract 1.0 g/l Tryptone 1.0 g/l NaNO3 0.689 g/l Na2HPO4 x 2 H2O 0.14 g/l KNO3 0.103 g/l MgSO4 x 7 H2O 0.1 g/l Nitrilotriacetic acid 0.1 g/l CaSO4 x 2 H2O 0.06 g/l NaCl 0.008 g/l MnSO4 x H2O 0.0022 g/l H3BO3 0.0005 g/l ZnSO4 x 7 H2O 0.0005 g/l FeCl3 x 6 H2O 0.00047 g/l CoCl2 x 6 H2O 4.6e-05 g/l CuSO4 x 5 H2O 2.5e-05 g/l Na2MoO4 x 2 H2O 2.5e-05 g/l Distilled water

Culture temp

@ref	Growth	Type	Temperature (°C)	Range
328	positive	growth	70	thermophilic
67770	positive	growth	70	thermophilic

Physiology and metabolism

Oxygen tolerance

@ref	Oxygen tolerance	Confidence
125439	facultative anaerobe	100

Compound production

@ref	Compound
328	restriction endonuclease TflI (TaqI)
20216	Endonuclease Taq I
20216	Endonuclease II

Observation

67770

Observationquinones: MK-8

Isolation, sampling and environmental information

Isolation source categories

Cat1	Cat2	Cat3
#Environmental	#Aquatic	#Thermal spring
#Condition	#Thermophilic (>45°C)	-

Isolation

@ref	Sample type	Geographic location	Country	Country ISO 3 Code	Continent
328	hot spring	Wyoming, Yellowstone Natl. Park	USA	USA	North America
67770	Mushroom Spring	Yellowstone National Park, WY	USA	USA	North America

Taxonmaps

69479

Global distribution of 16S sequence L09663 (>99% sequence identity) for Thermus aquaticus subclade from Microbeatlas

Aquatic Samples	74
Soil Samples	14
Animal Samples	12
Plant Samples
Total Samples	100

Interaction and safety

Risk assessment

@ref	Biosafety level	Biosafety level comment
328	1	Risk group (German classification) According to TRBA 466

Sequence information

Genome sequences

@ref	Description	Assembly level	INSDC accession	BV-BRC accession	IMG accession	NCBI tax ID	Score
67770	ASM128025v1 assembly for Thermus aquaticus YT-1	scaffold	GCA_001280255	271.14	2648501156	271	43.85

Genome browser: GCA_001280255

16S sequences

@ref	Description	Accession	Length	Database	NCBI tax ID
328	Thermus aquaticus strain YT-1 16S ribosomal RNA gene, partial sequence	L09663	1470		271

GC content

@ref	GC-content (mol%)
328	67.4
67770	67.4

Genome-based predictions

Predictions

Predictions from Deep Learning for Genome Sequence Data. R package version 0.3.1 based on: Thermus aquaticus YT-1
NCBI: GCA_001280255

@ref	Trait	Model	Prediction	Confidence in %	In training data
125439	spore_formation	BacteriaNetⓘ	yes	71.30	no
125439	motility	BacteriaNetⓘ	no	62.80	no
125439	gram_stain	BacteriaNetⓘ	positive	85.20	no
125439	oxygen_tolerance	BacteriaNetⓘ	facultative anaerobe	100.00	no

Predictions from bacterial phenotypic traits through improved machine learning using high-quality, curated datasets based on: Thermus aquaticus strain YT-1
PATRIC: 271.14

@ref	Trait	Model	Prediction	Confidence in %	In training data
125438	gram-positive	gram-positiveⓘ	no	75.15	no
125438	anaerobic	anaerobicⓘ	no	80.41	no
125438	spore-forming	spore-formingⓘ	no	71.10	no
125438	aerobic	aerobicⓘ	yes	73.36	no
125438	thermophilic	thermophileⓘ	yes	73.51	no
125438	flagellated	motile2+ⓘ	no	78.64	no

Literature

Title	Authors	Journal	DOI	Year
New-Generation Antibacterial Agent-Cellulose-Binding Thermostable TP84_Endolysin.	Ponikowska M, Zebrowska J, Skowron PM.	Int J Mol Sci	10.3390/ijms252313111	2024
Exploration of Deinococcus-Thermus molecular diversity by novel group-specific PCR primers.	Theodorakopoulos N, Bachar D, Christen R, Alain K, Chapon V.	Microbiologyopen	10.1002/mbo3.119	2013
Distribution of genes for synthesis of trehalose and Mannosylglycerate in Thermus spp. and direct correlation of these genes with halotolerance.	Alarico S, Empadinhas N, Simoes C, Silva Z, Henne A, Mingote A, Santos H, da Costa MS.	Appl Environ Microbiol	10.1128/aem.71.5.2460-2466.2005	2005
Properties of Thermus ruber Strains Isolated from Icelandic Hot Springs and DNA:DNA Homology of Thermus ruber and Thermus aquaticus.	Sharp RJ, Williams RA.	Appl Environ Microbiol	10.1128/aem.54.8.2049-2053.1988	1988
Isolation of Thermus strains from hot composts (60 to 80 degrees C).	Beffa T, Blanc M, Lyon PF, Vogt G, Marchiani M, Fischer JL, Aragno M.	Appl Environ Microbiol	10.1128/aem.62.5.1723-1727.1996	1996
Distribution, purification, and characterization of thermostable phenylalanine dehydrogenase from thermophilic actinomycetes.	Ohshima T, Takada H, Yoshimura T, Esaki N, Soda K.	J Bacteriol	10.1128/jb.173.13.3943-3948.1991	1991
Complex polar lipids of a hot spring cyanobacterial mat and its cultivated inhabitants.	Ward DM, Panke S, Kloppel KD, Christ R, Fredrickson H.	Appl Environ Microbiol	10.1128/aem.60.9.3358-3367.1994	1994
High-cell density shake-flask expression and rapid purification of the large fragment of Thermus aquaticus DNA polymerase I using a new chemically and temperature inducible expression plasmid in Escherichia coli.	Brandis JW, Johnson KA.	Protein Expr Purif	10.1016/j.pep.2008.09.018	2009
Current Status of the Degradation of Aliphatic and Aromatic Petroleum Hydrocarbons by Thermophilic Microbes and Future Perspectives.	Nzila A.	Int J Environ Res Public Health	10.3390/ijerph15122782	2018
A ring-like nucleoid is not necessary for radioresistance in the Deinococcaceae.	Zimmerman JM, Battista JR.	BMC Microbiol	10.1186/1471-2180-5-17	2005
Isocitrate dehydrogenase from Thermus aquaticus YT1: purification of the enzyme and cloning, sequencing, and expression of the gene.	Miyazaki K.	Appl Environ Microbiol	10.1128/aem.62.12.4627-4631.1996	1996
UV-induced crosslinks in the 16S rRNAs of Escherichia coli, Bacillus subtilis and Thermus aquaticus and their implications for ribosome structure and photochemistry.	Noah JW, Shapkina T, Wollenzien P.	Nucleic Acids Res	10.1093/nar/28.19.3785	2000
Effects of oxygen and methyl viologen on Thermus aquaticus.	MacMichael GJ.	J Bacteriol	10.1128/jb.170.10.4995-4998.1988	1988
The value of basic research: discovery of Thermus aquaticus and other extreme thermophiles.	Brock TD.	Genetics	10.1093/genetics/146.4.1207	1997
Effect of growth temperature on fatty acid composition of ten thermus strains.	Nordstrom KM, Laakso SV.	Appl Environ Microbiol	10.1128/aem.58.5.1656-1660.1992	1992
The 'endo-blue method' for direct cloning of restriction endonuclease genes in E. coli.	Fomenkov A, Xiao JP, Dila D, Raleigh E, Xu SY.	Nucleic Acids Res	10.1093/nar/22.12.2399	1994
Bacteriocinlike Activity within the Genus Thermus.	Becker RJ, Becker DA, Starzyk MJ.	Appl Environ Microbiol	10.1128/aem.52.5.1203-1205.1986	1986
Current State of Knowledge in Microbial Degradation of Polycyclic Aromatic Hydrocarbons (PAHs): A Review.	Ghosal D, Ghosh S, Dutta TK, Ahn Y.	Front Microbiol	10.3389/fmicb.2016.01369	2016
Characterization of ribonuclease P RNAs from thermophilic bacteria.	Brown JW, Haas ES, Pace NR.	Nucleic Acids Res	10.1093/nar/21.3.671	1993
Sequences of the 5S rRNAs of the thermo-acidophilic archaebacterium Sulfolobus solfataricus (Caldariella acidophila) and the thermophilic eubacteria Bacillus acidocaldarius and Thermus aquaticus.	Dams E, Londei P, Cammarano P, Vandenberghe A, De Wachter R.	Nucleic Acids Res	10.1093/nar/11.14.4667	1983
Glycolipids from some extreme thermophilic bacteria belonging to the genus Thermus.	Pask-Hughes RA, Shaw N.	J Bacteriol	10.1128/jb.149.1.54-58.1982	1982
Global Association between Thermophilicity and Vancomycin Susceptibility in Bacteria.	Roy C, Alam M, Mandal S, Haldar PK, Bhattacharya S, Mukherjee T, Roy R, Rameez MJ, Misra AK, Chakraborty R, Nanda AK, Mukhopadhyay SK, Ghosh W.	Front Microbiol	10.3389/fmicb.2016.00412	2016
GELRITE as a Gelling Agent in Media for the Growth of Thermophilic Microorganisms.	Lin CC, Casida LE.	Appl Environ Microbiol	10.1128/aem.47.2.427-429.1984	1984
Anaerobic growth, a property horizontally transferred by an Hfr-like mechanism among extreme thermophiles.	Ramirez-Arcos S, Fernandez-Herrero LA, Marin I, Berenguer J.	J Bacteriol	10.1128/jb.180.12.3137-3143.1998	1998
Fine structure of Thermus aquaticus, an extreme thermophile.	Brock TD, Edwards MR.	J Bacteriol	10.1128/jb.104.1.509-517.1970	1970
Effect of temperature on the fatty acid composition of Thermus aquaticus.	Ray PH, White DC, Brock TD.	J Bacteriol	10.1128/jb.106.1.25-30.1971	1971
Isolation of Halotolerant Thermus spp. from Submarine Hot Springs in Iceland.	Kristjansson JK, Hreggvidsson GO, Alfredsson GA.	Appl Environ Microbiol	10.1128/aem.52.6.1313-1316.1986	1986
Thermus aquaticus gen. n. and sp. n., a nonsporulating extreme thermophile.	Brock TD, Freeze H.	J Bacteriol	10.1128/jb.98.1.289-297.1969	1969
Anti-peptidyl transferase leader peptides of attenuation-regulated chloramphenicol-resistance genes.	Gu Z, Harrod R, Rogers EJ, Lovett PS.	Proc Natl Acad Sci U S A	10.1073/pnas.91.12.5612	1994
A conserved motif in S-layer proteins is involved in peptidoglycan binding in Thermus thermophilus.	Olabarria G, Carrascosa JL, de Pedro MA, Berenguer J.	J Bacteriol	10.1128/jb.178.16.4765-4772.1996	1996
Detection of Salmonella spp. in oysters by PCR.	Bej AK, Mahbubani MH, Boyce MJ, Atlas RM.	Appl Environ Microbiol	10.1128/aem.60.1.368-373.1994	1994
Characterization of exopolymers of aquatic bacteria by pyrolysis-mass spectrometry.	Ford T, Sacco E, Black J, Kelley T, Goodacre R, Berkeley RC, Mitchell R.	Appl Environ Microbiol	10.1128/aem.57.6.1595-1601.1991	1991
Compilation of 5S rRNA and 5S rRNA gene sequences.	Specht T, Wolters J, Erdmann VA.	Nucleic Acids Res	10.1093/nar/18.suppl.2215	1990
Insertion sequences.	Mahillon J, Chandler M.	Microbiol Mol Biol Rev	10.1128/mmbr.62.3.725-774.1998	1998
Error removal in microchip-synthesized DNA using immobilized MutS.	Wan W, Li L, Xu Q, Wang Z, Yao Y, Wang R, Zhang J, Liu H, Gao X, Hong J.	Nucleic Acids Res	10.1093/nar/gku405	2014
Selection criteria for SNP loci to maximize robustness of high-resolution melting analysis for plant breeding.	Yamagata Y, Yoshimura A, Anai T, Watanabe S.	Breed Sci	10.1270/jsbbs.18048	2018
Molecular cloning, expression, purification, and characterization of fructose-1,6-bisphosphate aldolase from Thermus aquaticus.	Sauve V, Sygusch J	Protein Expr Purif	10.1006/prep.2000.1380	2001
Crystallization and preliminary crystallographic analysis of an NADH oxidase that functions in peroxide reduction in Thermus aquaticus YT-1.	Mac Sweeney A, D'Arcy A, Higgins TM, Mayhew SG, Toomey D, Walsh MA	Acta Crystallogr D Biol Crystallogr	10.1107/S090744499800941X	1999
Purification and characterisation of NADH oxidase from Thermus aquaticus YT-1 and evidence that it functions in a peroxide-reduction system.	Toomey D, Mayhew SG	Eur J Biochem	10.1046/j.1432-1327.1998.2510935.x	1998
Cloning of genes of the aminopeptidase T family from Thermus thermophilus HB8 and Bacillus stearothermophilus NCIB8924: apparent similarity to the leucyl aminopeptidase family.	Motoshima H, Minagawa E, Tsukasaki F, Kaminogawa S	Biosci Biotechnol Biochem	10.1271/bbb.61.1710	1997
Cloning and analysis of the DNA polymerase-encoding gene from Thermus caldophilus GK24.	Kwon ST, Kim JS, Park JH, Kim HK, Lee DS	Mol Cells		1997
Molecular cloning and nucleotide sequence of the aminopeptidase T gene of Thermus aquaticus YT-1 and its high-level expression in Escherichia coli.	Motoshima H, Azuma N, Kaminogawa S, Ono M, Minagawa E, Matsuzawa H, Ohta T, Yamauchi K	Agric Biol Chem		1990
Enhancement of the thermostability of subtilisin E by introduction of a disulfide bond engineered on the basis of structural comparison with a thermophilic serine protease.	Takagi H, Takahashi T, Momose H, Inouye M, Maeda Y, Matsuzawa H, Ohta T	J Biol Chem	S0021-9258(19)39230-0	1990
NADH oxidase from the extreme thermophile Thermus aquaticus YT-1. Purification and characterisation.	Cocco D, Rinaldi A, Savini I, Cooper JM, Bannister JV	Eur J Biochem	10.1111/j.1432-1033.1988.tb14093.x	1988
The third restriction-modification system from Thermus aquaticus YT-1: solving the riddle of two TaqII specificities.	Skowron PM, Anton BP, Czajkowska E, Zebrowska J, Sulecka E, Krefft D, Jezewska-Frackowiak J, Zolnierkiewicz O, Witkowska M, Morgan RD, Wilson GG, Fomenkov A, Roberts RJ, Zylicz-Stachula A	Nucleic Acids Res	10.1093/nar/gkx599	2017
Highly conserved salt bridge stabilizes a proteinase K subfamily enzyme, Aqualysin I, from Thermus aquaticus YT-1.	Sakaguchi M, Osaku K, Maejima S, Ohno N, Sugahara Y, Oyama F, Kawakita M	AMB Express	10.1186/s13568-014-0059-2	2014
A novel exopolysaccharide from the biofilm of Thermus aquaticus YT-1 induces the immune response through Toll-like receptor 2.	Lin MH, Yang YL, Chen YP, Hua KF, Lu CP, Sheu F, Lin GH, Tsay SS, Liang SM, Wu SH	J Biol Chem	10.1074/jbc.M110.200113	2011
Stimulation of expression of a silica-induced protein (Sip) in Thermus thermophilus by supersaturated silicic acid.	Doi K, Fujino Y, Inagaki F, Kawatsu R, Tahara M, Ohshima T, Okaue Y, Yokoyama T, Iwai S, Ogata S	Appl Environ Microbiol	10.1128/AEM.02387-08	2009
Construction of an expression system for aqualysin I in Escherichia coli that gives a markedly improved yield of the enzyme protein.	Sakaguchi M, Niimiya K, Takezawa M, Toki T, Sugahara Y, Kawakita M	Biosci Biotechnol Biochem	10.1271/bbb.80132	2008
Role of disulphide bonds in a thermophilic serine protease aqualysin I from Thermus aquaticus YT-1.	Sakaguchi M, Takezawa M, Nakazawa R, Nozawa K, Kusakawa T, Nagasawa T, Sugahara Y, Kawakita M	J Biochem	10.1093/jb/mvn007	2008
Role of proline residues in conferring thermostability on aqualysin I.	Sakaguchi M, Matsuzaki M, Niimiya K, Seino J, Sugahara Y, Kawakita M	J Biochem	10.1093/jb/mvm025	2006
Quantification of intra- and extra-cellular thermophilic lipase/esterase production by Thermus sp.	Dominguez A, Sanroman A, Fucinos P, Rua ML, Pastrana L, Longo MA	Biotechnol Lett	10.1023/b:bile.0000024092.27943.75	2004
High-level expression, secretion, and purification of the thermostable aqualysin I from Thermus aquaticus YT-1 in Pichia pastoris.	Oledzka G, Dabrowski S, Kur J	Protein Expr Purif	10.1016/s1046-5928(03)00060-3	2003
Independently expressed N-terminal pro-domain of aqualysin I precursor complements the folding of its mature domain to active form in Escherichia coli.	Kim JY, Choi YL, Cho YS, Kim CH, Lee YC	J Basic Microbiol	10.1002/1521-4028(200206)42:3<181::AID-JOBM181>3.0.CO;2-8	2002
Requirement for the COOH-terminal pro-sequence in the translocation of aqualysin I across the cytoplasmic membrane in Escherichia coli.	Kim DW, Matsuzawa H	Biochem Biophys Res Commun	10.1006/bbrc.2000.3657	2000
Weakly bound calcium ions involved in the thermostability of aqualysin I, a heat-stable subtilisin-type protease of Thermus aquaticus YT-1.	Lin SJ, Yoshimura E, Sakai H, Wakagi T, Matsuzawa H	Biochim Biophys Acta	10.1016/s0167-4838(99)00140-5	1999
Identification and designing of the S3 site of aqualysin I, a thermophilic subtilisin-related serine protease.	Tanaka T, Matsuzawa H, Ohta T	J Biochem	10.1093/oxfordjournals.jbchem.a022380	1999
Identification, cloning and expression of p25, an AT-rich DNA-binding protein from the extreme thermophile, Thermus aquaticus YT-1.	Du X, Pene JJ	Nucleic Acids Res	10.1093/nar/27.7.1690	1999
Recombinant His-tagged DNA polymerase. II. Cloning and purification of Thermus aquaticus recombinant DNA polymerase (Stoffel fragment).	Dabrowski S, Kur J	Acta Biochim Pol		1998
P1 specificity of aqualysin I (a subtilisin-type serine protease) from Thermus aquaticus YT-1, using P1-substituted derivatives of Streptomyces subtilisin inhibitor.	Tanaka T, Matsuzawa H, Kojima S, Kumagai I, Miura K, Ohta T	Biosci Biotechnol Biochem	10.1271/bbb.62.2035	1998
Substrate Specificity of Aqualysin I Altered by an Organic Solvent, DMSO.	Tanaka T, Matsuzawa H, Ohta T	Biosci Biotechnol Biochem	10.1271/bbb.63.446	1999
Role of the COOH-terminal pro-sequence of aqualysin I (a heat-stable serine protease) in its extracellular secretion by Thermus thermophilus.	Kim DW, Lee YC, Matsuzawa H	FEMS Microbiol Lett	10.1111/j.1574-6968.1997.tb12750.x	1997
Substrate Specificity of Aqualysin I, a Bacterial Thermophilic Alkaline Serine Protease from Thermus aquaticus YT-1: Comparison with Proteinase K, Subtilisin BPN' and Subtilisin Carlsberg.	Tanaka T, Matsuzawa H, Ohta T	Biosci Biotechnol Biochem	10.1271/bbb.62.2161	1998
Stability of Thermostable Enzyme, Aqualysin I ; a Subtilisin-type Serine Protease from Thermus aquaticus YT-1.	Tanaka T, Matsuzawa H, Ohta T	Biosci Biotechnol Biochem	10.1271/bbb.62.1806	1998
Identification and characterization of a thermostable MutS homolog from Thermus aquaticus.	Biswas I, Hsieh P	J Biol Chem	10.1074/jbc.271.9.5040	1996
Thermophilic biodegradation of BTEX by two Thermus Species.	Chen CI, Taylor RT	Biotechnol Bioeng	10.1002/bit.260480609	1995
Expression of aqualysin I (a thermophilic protease) in soluble form in Escherichia coli under a bacteriophage T7 promoter.	Sakamoto S, Terada I, Iijima M, Ohta T, Matsuzawa H	Biosci Biotechnol Biochem	10.1271/bbb.59.1438	1995
A gene encoding a thermophilic alkaline serine proteinase from Thermus sp. strain Rt41A and its expression in Escherichia coli.	Munro GK, McHale RH, Saul DJ, Reeves RA, Bergquist PL	Microbiology (Reading)	10.1099/13500872-141-7-1731	1995
Cloning and overexpression of thermostable DNA polymerase gene in Escherichia coli.	Jin C, Liu H, Yang S, Zhang S	Chin J Biotechnol		1995
Fermentation conditions for efficient production of thermophilic protease in Escherichia coli harboring a plasmid.	Sakamoto S, Terada I, Iijima M, Matsuzawa H, Ohta T	Appl Microbiol Biotechnol	10.1007/BF00173922	1994
Overproduction of Thermus aquaticus DNA polymerase and its structural analysis by ion-spray mass spectrometry.	Ishino Y, Ueno T, Miyagi M, Uemori T, Imamura M, Tsunasawa S, Kato I	J Biochem	10.1093/oxfordjournals.jbchem.a124622	1994
Carboxypeptidase Taq, a thermostable zinc enzyme, from Thermus aquaticus YT-1: molecular cloning, sequencing, and expression of the encoding gene in Escherichia coli.	Lee SH, Taguchi H, Yoshimura E, Minagawa E, Kaminogawa S, Ohta T, Matsuzawa H	Biosci Biotechnol Biochem	10.1271/bbb.58.1490	1994
The recA gene from the thermophile Thermus aquaticus YT-1: cloning, expression, and characterization.	Angov E, Camerini-Otero RD	J Bacteriol	10.1128/jb.176.5.1405-1412.1994	1994
Polar lipids and fatty acid composition of Thermus strains from New Zealand.	Ferraz AS, Carreto L, Tenreiro S, Nobre MF, da Costa MS	Antonie Van Leeuwenhoek	10.1007/BF00882773	1994
Purification, crystallization and preliminary X-ray investigation of aqualysin I, a heat-stable serine protease.	Green PR, Oliver JD, Strickland LC, Toerner DR, Matsuzawa H, Ohta T	Acta Crystallogr D Biol Crystallogr	10.1107/S0907444992012083	1993
Purification and characterization of a thermostable carboxypeptidase (carboxypeptidase Taq) from Thermus aquaticus YT-1.	Lee SH, Minagawa E, Taguchi H, Matsuzawa H, Ohta T, Kaminogawa S, Yamauchi K	Biosci Biotechnol Biochem	10.1271/bbb.56.1839	1992
Involvement of NH2-terminal pro-sequence in the production of active aqualysin I (a thermophilic serine protease) in Escherichia coli.	Lee YC, Miyata Y, Terada I, Ohta T, Matsuzawa H	Agric Biol Chem		1991
Production and Extracellular Secretion of Aqualysin I (a Thermophilic Subtilisin-Type Protease) in a Host-Vector System for Thermus thermophilus.	Touhara N, Taguchi H, Koyama Y, Ohta T, Matsuzawa H	Appl Environ Microbiol	10.1128/aem.57.11.3385-3387.1991	1991
Molecular cloning and nucleotide sequence of 3-isopropylmalate dehydrogenase gene (leuB) from an extreme thermophile, Thermus aquaticus YT-1.	Kirino H, Oshima T	J Biochem	10.1093/oxfordjournals.jbchem.a123470	1991
Unique precursor structure of an extracellular protease, aqualysin I, with NH2- and COOH-terminal pro-sequences and its processing in Escherichia coli.	Terada I, Kwon ST, Miyata Y, Matsuzawa H, Ohta T	J Biol Chem	S0021-9258(19)39186-0	1990
Nucleotide sequence and characteristics of the gene for L-lactate dehydrogenase of Thermus aquaticus YT-1 and the deduced amino acid sequence of the enzyme.	Ono M, Matsuzawa H, Ohta T	J Biochem	10.1093/oxfordjournals.jbchem.a123004	1990
Crystallization and preliminary X-ray diffraction studies of intact EF-Tu from Thermus aquaticus YT-1.	Lippmann C, Betzel C, Dauter Z, Wilson K, Erdmann VA	FEBS Lett	10.1016/0014-5793(88)80355-7	1988
Determination of the positions of the disulfide bonds in aqualysin I (a thermophilic alkaline serine protease) of Thermus aquaticus YT-1.	Kwon ST, Matsuzawa H, Ohta T	J Biochem	10.1093/oxfordjournals.jbchem.a122509	1988
Nucleotide sequence of the gene for aqualysin I (a thermophilic alkaline serine protease) of Thermus aquaticus YT-1 and characteristics of the deduced primary structure of the enzyme.	Kwon ST, Terada I, Matsuzawa H, Ohta T	Eur J Biochem	10.1111/j.1432-1033.1988.tb14025.x	1988
Purification and characterization of aqualysin I (a thermophilic alkaline serine protease) produced by Thermus aquaticus YT-1.	Matsuzawa H, Tokugawa K, Hamaoki M, Mizoguchi M, Taguchi H, Terada I, Kwon ST, Ohta T	Eur J Biochem	10.1111/j.1432-1033.1988.tb13809.x	1988
Isolation and preliminary taxonomic studies of Thermus strains isolated from Yellowstone National Park, USA.	Munster MJ, Munster AP, Woodrow JR, Sharp RJ	J Gen Microbiol	10.1099/00221287-132-6-1677	1986
Fructose 1,6-bisphosphate-dependent L-lactate dehydrogenase from Thermus aquaticus YT-1, an extreme thermophile: activation by citrate and modification reagents and comparison with Thermus caldophilus GK24 L-lactate dehydrogenase.	Machida M, Matsuzawa H, Ohta T	J Biochem	10.1093/oxfordjournals.jbchem.a135132	1985
[Isolation and properties of DNA polymerase from the extreme thermophilic bacterium Thermus ruber].	Kaledin AS, Sliusarenko AG, Gorodetskii SI	Biokhimiia		1982
[Isolation and properties of DNA polymerase from extreme thermophylic bacteria Thermus aquaticus YT-1].	Kaledin AS, Sliusarenko AG, Gorodetskii SI	Biokhimiia		1980
Regulatory characteristics of phosphoenolpyruvate carboxylase from the extreme thermophile, Thermus aquaticus.	Sundaram TK, Bridger GP	Biochim Biophys Acta	10.1016/0005-2744(79)90160-8	1979
An isochizomer of TaqI from Thermus thermophilus HB8.	Sato S, Shinomiya T	J Biochem	10.1093/oxfordjournals.jbchem.a132252	1978
A comparative analysis of extreme thermophilic bacteria belonging to the genus Thermus.	Degryse E, Glansdorff N, Pierard A	Arch Microbiol	10.1007/BF00402307	1978
Thermus islandicus sp. nov., a mixotrophic sulfur-oxidizing bacterium isolated from the Torfajokull geothermal area.	Bjornsdottir SH, Petursdottir SK, Hreggvidsson GO, Skirnisdottir S, Hjorleifsdottir S, Arnfinnsson J, Kristjansson JK	Int J Syst Evol Microbiol	10.1099/ijs.0.007013-0	2009

References

#328	Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 625
#20215	Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )
#20216	Curators of the HKI: Collection Description Leibniz-Institut für Naturstoff-Forschung und Infektionsbiologie e. V. Hans-Knöll-Institut (HKI) . Leibniz-Institut für Naturstoff-Forschung und Infektionsbiologie e. V. Hans-Knöll-Institut (HKI):
#67770	Japan Collection of Microorganism (JCM) ; Curators of the JCM;
#69479	João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .
#125438	Julia Koblitz, Lorenz Christian Reimer, Rüdiger Pukall, Jörg Overmann: Predicting bacterial phenotypic traits through improved machine learning using high-quality, curated datasets. 2024 ( DOI 10.1101/2024.08.12.607695 )
#125439	Philipp Münch, René Mreches, Martin Binder, Hüseyin Anil Gündüz, Xiao-Yin To, Alice McHardy: deepG: Deep Learning for Genome Sequence Data. R package version 0.3.1 .
#126262	A. Lissin, I. Schober, J. F. Witte, H. Lüken, A. Podstawka, J. Koblitz, B. Bunk, P. Dawyndt, P. Vandamme, P. de Vos, J. Overmann, L. C. Reimer: StrainInfo—the central database for linked microbial strain identifiers. ( DOI 10.1093/database/baaf059 )

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Thermus aquaticus (DSM 625, ATCC 25104, YT-1)

Name and taxonomic classification

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Culture medium

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Physiology and metabolism

Oxygen tolerance

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Genome browser: GCA_001280255

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