Clostridium botulinum (CIP 104310, ATCC 25763)

Name: Clostridium botulinum (CIP 104310, ATCC 25763)
Creator: BacDive
License: http://creativecommons.org/licenses/by/4.0/

BacDive ID 166627

Type strain

Culture col. no. CIP 104310 ATCC 25763

NCBI tax ID(s) 1491

Special Collections CIP

Links

Clostridium botulinum CIP 104310 is a bacterium of the family Clostridiaceae.

genome sequence 16S sequence Bacteria

Name and taxonomic classification

SI-ID 87684

ATCC 25763,CIP 104310,VTT E-98821

@ref 20215

Last LPSN update 2026-02-09 11:40:22

Domain Bacteria

Phylum Bacillota

Class Clostridia

Order Eubacteriales

Family Clostridiaceae

Genus Clostridium

Species Clostridium botulinum

Full scientific name Clostridium botulinum (van Ermengem 1896) Bergey et al. 1923 (Approved Lists 1980)

Synonyms (1)

"Bacillus botulinus"

BacDive ID	Other strains from **Clostridium botulinum** (14)	Type strain
141845	C. botulinum CCUG 7735
141865	C. botulinum CCUG 7968, ATCC 19397, NCTC 7272
141866	C. botulinum CCUG 7969, NCTC 7273
141867	C. botulinum CCUG 7970, NCTC 8264
141868	C. botulinum CCUG 7971, NCTC 8265
141869	C. botulinum CCUG 7974, NCTC 8266
142345	C. botulinum CCUG 11910
142708	C. botulinum CCUG 14228
144075	C. botulinum CCUG 22775
146789	C. botulinum CCUG 32121
146790	C. botulinum CCUG 32122
146791	C. botulinum CCUG 32123
149343	C. botulinum CCUG 37546
150472	C. botulinum CCUG 41389

Morphology

Culture and growth conditions

Physiology and metabolism

Oxygen tolerance

@ref	Oxygen tolerance	Confidence
125439	facultative anaerobe	97.8

Isolation, sampling and environmental information

Interaction and safety

Risk assessment

@ref	Biosafety level	Biosafety level comment
20215	2	Risk group

Sequence information

Genome sequences

@ref	Description	Assembly level	INSDC accession	BV-BRC accession	IMG accession	NCBI tax ID	Score
66792	CBOT009-SEQ-1-ASM-1 assembly for Clostridium botulinum ATCC 25763	contig	GCA_001276985	1491.447	2660237905	1491	73.93
66792	ASM1101796v1 assembly for Clostridium botulinum ATCC 25763	contig	GCA_011017965	1491.2181	8114561737	1491	63.17

16S sequences

@ref	Description	Accession	Length	Database	NCBI tax ID
20215	Clostridium botulinum A 16S ribosomal RNA (16S rRNA) gene	L37585	1453		36826

Genome-based predictions

Predictions

Predictions from Deep Learning for Genome Sequence Data. R package version 0.3.1 based on: Clostridium botulinum ATCC 25763
NCBI: GCA_001276985

@ref	Trait	Model	Prediction	Confidence in %	In training data
125439	spore_formation	BacteriaNetⓘ	yes	66.90	no
125439	motility	BacteriaNetⓘ	yes	71.30	no
125439	gram_stain	BacteriaNetⓘ	positive	88.60	no
125439	oxygen_tolerance	BacteriaNetⓘ	facultative anaerobe	97.80	no

Predictions from bacterial phenotypic traits through improved machine learning using high-quality, curated datasets based on: Clostridium botulinum strain ATCC 25763
PATRIC: 1491.447

@ref	Trait	Model	Prediction	Confidence in %	In training data
125438	gram-positive	gram-positiveⓘ	yes	65.47	no
125438	anaerobic	anaerobicⓘ	yes	86.26	no
125438	spore-forming	spore-formingⓘ	yes	68.12	no
125438	aerobic	aerobicⓘ	no	96.37	no
125438	thermophilic	thermophileⓘ	no	89.21	no
125438	flagellated	motile2+ⓘ	yes	78.76	no

Literature

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	First report of an infant botulism case due to Clostridium botulinum type Af.	de Jong LI, Fernandez RA, Pareja V, Giaroli G, Guidarelli SR, Dykes JK, Luquez C.	J Clin Microbiol	10.1128/jcm.02894-14	2015
	High quality draft genome sequence of Mycoplasma testudineum strain BH29^T, isolated from the respiratory tract of a desert tortoise.	Weitzman CL, Tillett RL, Sandmeier FC, Tracy CR, Alvarez-Ponce D.	Stand Genomic Sci	10.1186/s40793-018-0309-z	2018
	High quality draft genome sequences of Mycoplasma agassizii strains PS6^T and 723 isolated from Gopherus tortoises with upper respiratory tract disease.	Alvarez-Ponce D, Weitzman CL, Tillett RL, Sandmeier FC, Tracy CR.	Stand Genomic Sci	10.1186/s40793-018-0315-1	2018
Phylogeny	Differentiation of Clostridium botulinum serotype A strains by multiple-locus variable-number tandem-repeat analysis.	Macdonald TE, Helma CH, Ticknor LO, Jackson PJ, Okinaka RT, Smith LA, Smith TJ, Hill KK.	Appl Environ Microbiol	10.1128/aem.01539-07	2008
Phylogeny	Efficient DNA fingerprinting of Clostridium botulinum types A, B, E, and F by amplified fragment length polymorphism analysis.	Keto-Timonen R, Nevas M, Korkeala H.	Appl Environ Microbiol	10.1128/aem.71.3.1148-1154.2005	2005
	Neurotoxin gene clusters in Clostridium botulinum type Ab strains.	Luquez C, Raphael BH, Maslanka SE.	Appl Environ Microbiol	10.1128/aem.01009-09	2009
Phylogeny	Multiplex PCR assay for detection and identification of Clostridium botulinum types A, B, E, and F in food and fecal material.	Lindstrom M, Keto R, Markkula A, Nevas M, Hielm S, Korkeala H.	Appl Environ Microbiol	10.1128/aem.67.12.5694-5699.2001	2001
Phylogeny	Diversity of proteolytic Clostridium botulinum strains, determined by a pulsed-field gel electrophoresis approach.	Nevas M, Lindstrom M, Hielm S, Bjorkroth KJ, Peck MW, Korkeala H.	Appl Environ Microbiol	10.1128/aem.71.3.1311-1317.2005	2005
Phylogeny	Identification of Clostridium species and DNA fingerprinting of Clostridium perfringens by amplified fragment length polymorphism analysis.	Keto-Timonen R, Heikinheimo A, Eerola E, Korkeala H.	J Clin Microbiol	10.1128/jcm.01275-06	2006
	Independent evolution of neurotoxin and flagellar genetic loci in proteolytic Clostridium botulinum.	Carter AT, Paul CJ, Mason DR, Twine SM, Alston MJ, Logan SM, Austin JW, Peck MW.	BMC Genomics	10.1186/1471-2164-10-115	2009
Transcriptome	The purine-utilizing bacterium Clostridium acidurici 9a: a genome-guided metabolic reconsideration.	Hartwich K, Poehlein A, Daniel R.	PLoS One	10.1371/journal.pone.0051662	2012
Phylogeny	Enumeration and isolation of cpe-positive Clostridium perfringens spores from feces.	Heikinheimo A, Lindstrom M, Korkeala H.	J Clin Microbiol	10.1128/jcm.42.9.3992-3997.2004	2004
Enzymology	First case of type E wound botulism diagnosed using real-time PCR.	Artin I, Bjorkman P, Cronqvist J, Radstrom P, Holst E.	J Clin Microbiol	10.1128/jcm.01192-07	2007
	Genetic diversity among Botulinum Neurotoxin-producing clostridial strains.	Hill KK, Smith TJ, Helma CH, Ticknor LO, Foley BT, Svensson RT, Brown JL, Johnson EA, Smith LA, Okinaka RT, Jackson PJ, Marks JD.	J Bacteriol	10.1128/jb.01180-06	2007
Phylogeny	Development of a combined selection and enrichment PCR procedure for Clostridium botulinum Types B, E, and F and its use to determine prevalence in fecal samples from slaughtered pigs.	Dahlenborg M, Borch E, Radstrom P.	Appl Environ Microbiol	10.1128/aem.67.10.4781-4788.2001	2001
Biotechnology	PCR and gene probe identification of botulinum neurotoxin A-, B-, E-, F-, and G-producing Clostridium spp. and evaluation in food samples.	Fach P, Gibert M, Griffais R, Guillou JP, Popoff MR.	Appl Environ Microbiol	10.1128/aem.61.1.389-392.1995	1995
Biotechnology	Detection of enterotoxigenic Clostridium perfringens in food and fecal samples with a duplex PCR and the slide latex agglutination test.	Fach P, Popoff MR.	Appl Environ Microbiol	10.1128/aem.63.11.4232-4236.1997	1997
Metabolism	Dependence of Clostridium botulinum gas and protease production on culture conditions.	Montville TJ.	Appl Environ Microbiol	10.1128/aem.45.2.571-575.1983	1983
	Metabiotic effect of Bacillus licheniformis on Clostridium botulinum: implications for home-canned tomatoes.	Montville TJ.	Appl Environ Microbiol	10.1128/aem.44.2.334-338.1982	1982
Biotechnology	Growth and toxin production by Clostridium botulinum in moldy tomato juice.	Huhtanen CN, Naghski J, Custer CS, Russell RW.	Appl Environ Microbiol	10.1128/aem.32.5.711-715.1976	1976
	Identification of capsule-forming Bacillus anthracis spores with the PCR and a novel dual-probe hybridization format.	Reif TC, Johns M, Pillai SD, Carl M.	Appl Environ Microbiol	10.1128/aem.60.5.1622-1625.1994	1994
Phylogeny	Electrophoretic study of Clostridium species.	Cato EP, Hash DE, Holdeman LV, Moore WE.	J Clin Microbiol	10.1128/jcm.15.4.688-702.1982	1982
Cultivation	Effect of plating medium on heat activation requirement of Clostridium botulinum spores.	Montville TJ	Appl Environ Microbiol	10.1128/aem.42.4.734-736.1981	1981

References

#20215	Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )
#66792	Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .
#125438	Julia Koblitz, Lorenz Christian Reimer, Rüdiger Pukall, Jörg Overmann: Predicting bacterial phenotypic traits through improved machine learning using high-quality, curated datasets. 2024 ( DOI 10.1101/2024.08.12.607695 )
#125439	Philipp Münch, René Mreches, Martin Binder, Hüseyin Anil Gündüz, Xiao-Yin To, Alice McHardy: deepG: Deep Learning for Genome Sequence Data. R package version 0.3.1 .
#126262	A. Lissin, I. Schober, J. F. Witte, H. Lüken, A. Podstawka, J. Koblitz, B. Bunk, P. Dawyndt, P. Vandamme, P. de Vos, J. Overmann, L. C. Reimer: StrainInfo—the central database for linked microbial strain identifiers. ( DOI 10.1093/database/baaf059 )

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Clostridium botulinum (CIP 104310, ATCC 25763)

Name and taxonomic classification

Morphology

Culture and growth conditions

Physiology and metabolism

Oxygen tolerance

Isolation, sampling and environmental information

Interaction and safety

Risk assessment

Sequence information

Genome sequences

Genome browser: GCA_001276985

16S sequences

Genome-based predictions

Predictions

Literature

Literature

References

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