Name: Metallosphaera prunae DSM 10039
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 16647

Type strain:

Species: Metallosphaera prunae

Culture col. no.: DSM 10039, Ron 12/II

Strain history: <- K. O. Stetter; Ron 12/II <- T. Fuchs and H. Huber

NCBI tax ID(s): 47304 (species)

SI-ID 48738

DSM 10039

Section

Metallosphaera prunae DSM 10039 is a thermophilic archaeon that was isolated from smoldering slag heap, uranium mine.

thermophilic
16S sequence
Archaea
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Archaea"
	Phylum	*Thermoproteota*
	Class	*Thermoprotei*
	Order	*Sulfolobales*
	Family	*Sulfolobaceae*
	Genus	*Metallosphaera*
	Species	**Metallosphaera prunae**
	Full Scientific Name (LPSN)	Metallosphaera prunae Fuchs et al. 1996

Information on culture and growth conditions Culture and growth conditions

[Ref.: #3760]

Culture medium

SULFOLOBUS MEDIUM (DSMZ Medium 88)

[Ref.: #3760]

Culture medium growth

[Ref.: #3760]

Culture medium link

Medium recipe at MediaDive

[Ref.: #3760]

Culture medium composition

expand / minimize

	Temperatures	Kind of temperature		Temperature
[Ref.: #3760]			growth	75 °C

[Ref.: #3760]

Temperature range

thermophilic

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #3760]	Sample type/isolated from	smoldering slag heap, uranium mine
[Ref.: #3760]	Geographic location (country and/or sea, region)	Ronneburg
[Ref.: #3760]	Country	Germany
[Ref.: #3760]	Country ISO 3 Code	DEU
[Ref.: #3760]	Continent	Europe
* marker position based on {}

Isolation sources categories

#Engineered	#Other	#Mine
#Engineered	#Waste	#Solid waste
#Condition	#Thermophilic (>45°C)	-

What are isolation sources categories?

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #3760]

Biosafety level

Risk group (German classification)
According to TRBA 466

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #3760]	M.prunae DNA for 16S ribosomal RNA gene	X90482	1420	ENA	47304 tax ID

Genome sequence information:

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #66792]	Metallosphaera prunae strain Ron 12/II	47304.5	complete	PATRIC	47304 tax ID	*

[Ref.: #3760]	GC-content	47 mol%	high performance liquid chromatography (HPLC)
[Ref.: #3760]	GC-content	45 mol%	thermal denaturation, midpoint method (Tm)

Data predicted using genome information as a basis Genome-based predictions

	Trait	Prediction	Confidence in %	In training data
	Predictions from Diaspora project based on: Metallosphaera prunae strain Ron 12/II PATRIC: 47304.5
[Ref.: #69480]	gram-positive	no	96.56	no
[Ref.: #69480]	anaerobic	yes	85.562	no
[Ref.: #69480]	halophile	no	84.604	no
[Ref.: #69480]	spore-forming	no	96.48	no
[Ref.: #69480]	glucose-util	yes	81.131	no
[Ref.: #69480]	aerobic	no	79.397	no
[Ref.: #69480]	flagellated	no	97.744	no
[Ref.: #69480]	thermophile	yes	100	yes
[Ref.: #69480]	motile	no	84.313	no
[Ref.: #69480]	glucose-ferment	no	83.207	no

Availability in culture collections External links

[Ref.: #3760]

Culture collection no.

DSM 10039, Ron 12/II

[Ref.: #85668]

SI-ID 48738

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Metallosphaera cuprina sp. nov., an acidothermophilic, metal-mobilizing archaeon.	Liu LJ, You XY, Guo X, Liu SJ, Jiang CY	Int J Syst Evol Microbiol	10.1099/ijs.0.026591-0	2010	*
Metabolism	Metallosphaera sedula TA-2, a calditoglycerocaldarchaeol deletion strain of a thermoacidophilic archaeon.	Itoh YH, Kurosawa N, Uda I, Sugai A, Tanoue S, Itoh T, Horiuchi T, Itoh T	Extremophiles	10.1007/s007920100195	2001	*

References

#3760

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 10039

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#69480

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#85668

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID48738.1 )

* These data were automatically processed and therefore are not curated

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Metallosphaera prunae strain Ron 12/II

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