Metallosphaera hakonensis (DSM 7519, HO1-1, JCM 8857)

Name: Metallosphaera hakonensis (DSM 7519, HO1-1, JCM 8857)
Creator: BacDive
License: http://creativecommons.org/licenses/by/4.0/

BacDive ID 16646

Type strain

Strain Designation HO1-1

Culture col. no. DSM 7519 HO1-1 JCM 8857 ATCC 51241 IAM 14250

NCBI tax ID(s) 1293036 79601

Special Collections DSMZ JCM

History <- S. Takayanagi, Toho University School of Medicine, Ota-ku, Tokyo, Japan; HO1-1 <- S. Takayanagi and K. Sugimori; {1985} S. Takayanagi HO 1-1.

Links

Metallosphaera hakonensis HO1-1 is a thermophilic prokaryote that was isolated from hot spring.

thermophilic genome sequence 16S sequence

Name and taxonomic classification

SI-ID 42873

ATCC 51241,DSM 7519,IAM 14250,JCM 8857

@ref 20215

Last LPSN update 2026-02-09 11:22:01

Domain Archaea

Phylum Thermoproteota

Class Thermoprotei

Order Sulfolobales

Family Sulfolobaceae

Genus Metallosphaera

Species Metallosphaera hakonensis

Full scientific name Metallosphaera hakonensis (Takayanagi et al. 1996) Kurosawa et al. 2003

Synonyms (1)

Sulfolobus hakonensis

Morphology

Colony morphology

3197

Incubation period3-7 days

Culture and growth conditions

Culture medium

@ref	Name	Growth	Medium link	Composition
3197	SULFOLOBUS MEDIUM (DSMZ Medium 88)		Medium recipe at MediaDive	Name: SULFOLOBUS MEDIUM (DSMZ Medium 88) Composition: (NH4)2SO4 1.28713 g/l Yeast extract 0.990099 g/l KH2PO4 0.277228 g/l MgSO4 x 7 H2O 0.247525 g/l CaCl2 x 2 H2O 0.0693069 g/l FeCl3 x 6 H2O 0.019802 g/l Na2B4O7 x 10 H2O 0.00445545 g/l MnCl2 x 4 H2O 0.00178218 g/l ZnSO4 x 7 H2O 0.000217822 g/l CuCl2 x 2 H2O 4.95049e-05 g/l Na2MoO4 x 2 H2O 2.9703e-05 g/l VOSO4 x 2 H2O 2.9703e-05 g/l CoSO4 x 7 H2O 9.90099e-06 g/l Distilled water

Culture temp

@ref	Growth	Type	Temperature (°C)	Range
3197	positive	growth	65	thermophilic
67770	positive	growth	70	thermophilic

Physiology and metabolism

Oxygen tolerance

@ref	Oxygen tolerance	Confidence
125439	anaerobe	92.9

Isolation, sampling and environmental information

Isolation source categories

Cat1	Cat2	Cat3
#Environmental	#Aquatic	#Thermal spring
#Condition	#Thermophilic (>45°C)	-

Isolation

@ref	Sample type	Geographic location	Country	Country ISO 3 Code	Continent
3197	hot spring	Kanagawa, Ohwakudani area, Hakone Natl. Park	Japan	JPN	Asia
67770	Lava field at Owakudani	Hakone	Japan	JPN	Asia

Taxonmaps

69479

Global distribution of 16S sequence D86414 (>99% sequence identity) for Metallosphaera hakonensis subclade from Microbeatlas

Aquatic Samples	7
Soil Samples
Animal Samples
Plant Samples
Total Samples	7

Interaction and safety

Risk assessment

@ref	Biosafety level	Biosafety level comment
3197	1	Risk group (German classification) According to TRBA 466

Sequence information

Genome sequences

@ref	Description	Assembly level	INSDC accession	BV-BRC accession	IMG accession	NCBI tax ID	Score
66792	Metallosphaera hakonensis strain HO1-1	complete		79601.6		79601	99.8
66792	ASM320167v2 assembly for Metallosphaera hakonensis JCM 8857 = DSM 7519 HO1-1	complete	GCA_003201675	1293036.8		1293036	95.36
66792	ASM131582v1 assembly for Metallosphaera hakonensis JCM 8857 = DSM 7519	contig	GCA_001315825	1293036.3	2681813025	1293036	23.8

16S sequences

@ref	Description	Accession	Length	Database	NCBI tax ID
20218	Metallosphaera hakonensis gene for 16S rRNA, partial sequence	D86414	1461		79601
3197	Sulfolobus sp. H 16S rRNA	D14052	1446		359275

GC content

@ref	GC-content (mol%)	Method
3197	46.2	high performance liquid chromatography (HPLC)
67770	38.4	Buoyant density centrifugation (BD)

Genome-based predictions

Predictions

Predictions from Deep Learning for Genome Sequence Data. R package version 0.3.1 based on: Metallosphaera hakonensis strain HO1-1
PATRIC: 79601.6

@ref	Trait	Model	Prediction	Confidence in %	In training data
125439	oxygen_tolerance	BacteriaNetⓘ	anaerobe	92.90	no
125439	gram_stain	BacteriaNetⓘ	variable	84.80	no
125439	motility	BacteriaNetⓘ	yes	67.30	no
125439	spore_formation	BacteriaNetⓘ	yes	81.30	no

Predictions from bacterial phenotypic traits through improved machine learning using high-quality, curated datasets based on: Metallosphaera hakonensis JCM 8857 = DSM 7519 HO1-1
NCBI: GCA_003201675.2

@ref	Trait	Model	Prediction	Confidence in %	In training data
125438	gram-positive	gram-positiveⓘ	no	75.07	no
125438	anaerobic	anaerobicⓘ	yes	65.65	no
125438	aerobic	aerobicⓘ	no	59.41	no
125438	spore-forming	spore-formingⓘ	no	89.30	no
125438	thermophilic	thermophileⓘ	yes	78.68	yes
125438	flagellated	motile2+ⓘ	no	87.50	no

Literature

Topic	Title	Authors	Journal	DOI	Year
Genetics	Chalcopyrite bioleaching efficacy by extremely thermoacidophilic archaea leverages balanced iron and sulfur biooxidation.	Manesh MJH, Willard DJ, John KM, Kelly RM.	Bioresour Technol	10.1016/j.biortech.2024.131198	2024
	Complete Genome Sequences of Extremely Thermoacidophilic Metal-Mobilizing Type Strain Members of the Archaeal Family Sulfolobaceae, Acidianus brierleyi DSM-1651, Acidianus sulfidivorans DSM-18786, and Metallosphaera hakonensis DSM-7519.	Counts JA, Vitko NP, Kelly RM.	Microbiol Resour Announc	10.1128/mra.00831-18	2018
	Species-Specific Recognition of Sulfolobales Mediated by UV-Inducible Pili and S-Layer Glycosylation Patterns.	van Wolferen M, Shajahan A, Heinrich K, Brenzinger S, Black IM, Wagner A, Briegel A, Azadi P, Albers SV.	mBio	10.1128/mbio.03014-19	2020
	A clade of RHH proteins ubiquitous in Sulfolobales and their viruses regulates cell cycle progression.	Xuyang L, Cristina LM, Laura MA, Xu P.	Nucleic Acids Res	10.1093/nar/gkad011	2023
Genetics	Comparative Genomic Analysis Reveals the Metabolism and Evolution of the Thermophilic Archaeal Genus Metallosphaera.	Wang P, Li LZ, Qin YL, Liang ZL, Li XT, Yin HQ, Liu LJ, Liu SJ, Jiang CY.	Front Microbiol	10.3389/fmicb.2020.01192	2020
Genetics	Phenotype-driven assessment of the ancestral trajectory of sulfur biooxidation in the thermoacidophilic archaea Sulfolobaceae.	Willard DJ, H Manesh MJ, Bing RG, Alexander BH, Kelly RM.	mBio	10.1128/mbio.01033-24	2024
Enzymology	Enzymes Catalyzing Crotonyl-CoA Conversion to Acetoacetyl-CoA During the Autotrophic CO₂ Fixation in Metallosphaera sedula.	Liu L, Huber H, Berg IA.	Front Microbiol	10.3389/fmicb.2020.00354	2020
Phylogeny	Reclassification of Sulfolobus hakonensis Takayanagi et al. 1996 as Metallosphaera hakonensis comb. nov. based on phylogenetic evidence and DNA G+C content.	Kurosawa N, Itoh YH, Itoh T	Int J Syst Evol Microbiol	10.1099/ijs.0.02716-0	2003
Phylogeny	Metallosphaera cuprina sp. nov., an acidothermophilic, metal-mobilizing archaeon.	Liu LJ, You XY, Guo X, Liu SJ, Jiang CY	Int J Syst Evol Microbiol	10.1099/ijs.0.026591-0	2010

References

#3197	Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 7519
#20215	Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )
#20218	Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.: StrainInfo introduces electronic passports for microorganisms.. Syst Appl Microbiol. 37: 42 - 50 2014 ( DOI 10.1016/j.syapm.2013.11.002 , PubMed 24321274 )
#66792	Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .
#67770	Japan Collection of Microorganism (JCM) ; Curators of the JCM;
#69479	João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .
#125438	Julia Koblitz, Lorenz Christian Reimer, Rüdiger Pukall, Jörg Overmann: Predicting bacterial phenotypic traits through improved machine learning using high-quality, curated datasets. 2024 ( DOI 10.1101/2024.08.12.607695 )
#125439	Philipp Münch, René Mreches, Martin Binder, Hüseyin Anil Gündüz, Xiao-Yin To, Alice McHardy: deepG: Deep Learning for Genome Sequence Data. R package version 0.3.1 .
#126262	A. Lissin, I. Schober, J. F. Witte, H. Lüken, A. Podstawka, J. Koblitz, B. Bunk, P. Dawyndt, P. Vandamme, P. de Vos, J. Overmann, L. C. Reimer: StrainInfo—the central database for linked microbial strain identifiers. ( DOI 10.1093/database/baaf059 )

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Metallosphaera hakonensis (DSM 7519, HO1-1, JCM 8857)

Name and taxonomic classification

Morphology

Colony morphology

Culture and growth conditions

Culture medium

Culture temp

Physiology and metabolism

Oxygen tolerance

Isolation, sampling and environmental information

Isolation source categories

Isolation

Taxonmaps

Interaction and safety

Risk assessment

Sequence information

Genome sequences

Genome browser: GCA_003201675

16S sequences

GC content

Genome-based predictions

Predictions

Literature

Literature

References

BacDive

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