Nonomuraea dietziae (DSM 44320, ATCC 35861, IFO 14309)

Name: Nonomuraea dietziae (DSM 44320, ATCC 35861, IFO 14309)
Creator: BacDive
License: http://creativecommons.org/licenses/by/4.0/

BacDive ID 16558

Type strain

Strain Designation FH 2205, FH2205

Culture col. no. DSM 44320 ATCC 35861 IFO 14309 NBRC 14309 NRRL 11111 11 more

NCBI tax ID(s) 65515

Special Collections DSMZ JCM KCTC CIP Wink compendium JMRC

History DSMZ <- J. Wink, Hoechst AG, FH 2205 <- NRRL (Sebekia benihana) JCM <-- A. Shimazu <-- IFO 14309 <-- Upjohn Co., USA; UC 5762. CIP <- 2001, DSMZ <- J. Wink: strain FH2205

Links

Nonomuraea dietziae FH 2205 is a spore-forming, Gram-positive, filament-shaped bacterium that builds an aerial mycelium and was isolated from soil.

spore-forming Gram-positive filament-shaped genome sequence 16S sequence Bacteria

Name and taxonomic classification

SI-ID 41341

ATCC 35861,IFO 14309,DSM 44320,NRRL 11111,JCM 3338,DSM 1326,KCTC 9173,KCTC 9610,CIP 107127,NBRC 14309,BCRC 13419,CCRC 13419,BCRC 16340,CCRC 16340,CGMCC 4.1204

@ref 20215

Last LPSN update 2026-05-29 08:28:48

Domain Bacteria

Phylum Actinomycetota

Class Actinomycetes

Order Streptosporangiales

Family Streptosporangiaceae

Genus Nonomuraea

Species Nonomuraea dietziae

Full scientific name Nonomuraea dietziae corrig. Stackebrandt et al. 2001

Synonyms (2)

Nonomuraea dietzii
"Sebekia benihana"

Morphology

Cell morphology

@ref	Gram stain	Cell shape	Motility	Confidence
125439	positive			96.479
118933	positive	filament-shaped

Colony morphology

@ref	Colony color	Incubation period	Medium used
20044	Brown	10-14 days	ISP 2
20044	Beige	10-14 days	ISP 3
20044	Pink	10-14 days	ISP 4
20044	Rose	10-14 days	ISP 5
20044	Rose	10-14 days	ISP 6
20044	Pink	10-14 days	ISP 7

Multicellular morphology

@ref	Complex name	Complex color	Medium name
20044	Aerial Mycelium	Red brown (8012)	ISP 2
20044	Aerial Mycelium	Beige (1001)	ISP 3
20044	Aerial Mycelium	Pink	ISP 4
20044	Aerial Mycelium	Antique rose (3014)	ISP 5
20044	Aerial Mycelium	White	ISP 6
20044	Aerial Mycelium	Pink	ISP 7

Multimedia

@ref:	11668
multimedia content:	DSM_44320.jpg
multimedia.multimedia content:	https://www.dsmz.de/microorganisms/photos/DSM_44320.jpg
caption:	Medium 65 28°C
intellectual property rights:	© Leibniz-Institut DSMZ
manual_annotation:	1

Culture and growth conditions

Culture medium

@ref	Name	Medium link	Composition
11668	GYM STREPTOMYCES MEDIUM (DSMZ Medium 65)	Medium recipe at MediaDive	Name: GYM STREPTOMYCES MEDIUM (DSMZ Medium 65) Composition: Agar 18.0 g/l Malt extract 10.0 g/l Yeast extract 4.0 g/l Glucose 4.0 g/l CaCO3 2.0 g/l Distilled water
20044	ISP 2		Name: ISP 2 / Yeast Malt Agar (5265); 5265 Composition Malt extract 10.0 g/l Yeast extract 4.0 g/l Glucose 4.0 g/l Agar 15.0 g/l Preparation: Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.0 Usage: Maintenance and Taxonomy Organisms: All Actinomycetes
20044	ISP 3		Name: ISP 3; 5315 Composition Dog oat flakes 20.0 g/l Trace element solution (5314) 2.5 ml/l Agar 18.0 g/l Preparation: Oat flakes are cooked for 20 minutes, trace element solution and agar are added (in the case of non rolled oat flakes the suspension has to bee filtrated). Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.8 Usage: Maintenance and taxonomy (e.g. SEM As liquid medium for metabolite production) Organisms: All Actinomycetes Trace element solution 5314 Name: Trace element solution 5314; 5314 Composition CaCl2 x H2O 3.0 g/l Fe-III-citrate 1.0 g/l MnSO4 0.2 g/l ZnCl2 0.1 g/l CuSO4 x 5 H2O 0.025 g/l Sodium tetra borate 0.2 g/l CoCl2 x 6 H2O 0.004 g/l Sodium molybdate 0.01 g/l Preparation: Use double destillated water. Sterilisation: 20 minutes at 121°C pH before sterilisation: Usage: Trace element solution for different media Organisms:
20044	ISP 4		Name: ISP 4; DSM 547 Solution I: Difco soluble starch, 10.0 g. Make a paste of the starch with a small amount of cold distilled water and bring to a volume of 500 ml. Solution II: CaCO3 2.0 g K2HPO4 (anhydrous) 1.0 g MgSO4 x 7 H2O 1.0 g NaCl 1.0 g (NH4)2SO4 2.0 g Distilled water 500.0 ml Trace salt solution (see below) 1.0 ml The pH should be between 7.0 and 7.4. Do not adjust if it is within this range. Mix solutions I and II together. Add 20.0 g agar. Liquify agar by steaming at 100°C for 10 to 20 min. Trace element solution: FeSO4 x 7 H2O 0.1 g MnCl2 x 4 H2O 0.1 g ZnSO4 x 7 H2O 0.1 g Distilled water 100.0 ml
20044	ISP 5		Name: ISP 5 (5323) Composition L-Asparagine 1.0 g/l Glycerol 10.0 g/l K2HPO4 1.0 g/l Salt solution (see preparation) 1.0 ml/l Agar 20.0 g/l Preparation: Salt solution 1.0 g FeSO4 x 7 H2O 1.0 g MnCl2 x 4 H2O 1.0 g ZNSO4 x 7 H2O in 100 ml water Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.2 Usage: Maintenance and taxonomy Organisms: All Actinomycetes
20044	ISP 6		Name: ISP 6 (5318) Composition Peptone 15.0 g/l Proteose peptose 5.0 g/l Ferric ammonium citrate 0.5 g/l Sodium glycerophosphate 1.0 g/l Sodium thiosulfate 0.08 g/l Yeast extract 1.0 g/l Agar 15.0 g/l Sterilisation: 20 minutes at 121°C pH before sterilisation: Usage: Production of melanoid pigments Organisms: All Actinomycetes
20044	ISP 7		Name: ISP 7 (5322) Composition Glycerol 15.0 g/l L-Tyrosine 0.5 g/l L-Asparagine 1.0 g/l K2HPO4 0.5 g/l NaCl 0.5 g/l FeSO4 x 7 H2O 0.01 g/l Trace element solution 5343 1.0 ml/l Agar 20.0 Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.3 Usage: Production of melanoid pigments Organisms: All Actinomycetes
38196	MEDIUM 57 - for Streptomyces, Nocardioides, Lentzea albidocapillata and Streptoverticillium reticulum		Distilled water make up to (1000.000 ml);Agar (15.000 g);Glucose (4.000g);Yeast extract (4.000 g);Malt extract (10.000 g);Calcium carbonate (2.000 g)
11668	N-Z-AMINE-MEDIUM (DSMZ Medium 554)	Medium recipe at MediaDive	Name: N-Z-AMINE-MEDIUM (DSMZ Medium 554) Composition: Starch 20.0 g/l Agar 20.0 g/l Glucose 10.0 g/l N-Z amine 5.0 g/l Yeast extract 5.0 g/l CaCO3 1.0 g/l Distilled water
118933	CIP Medium 57	Medium recipe at CIP

Culture temp

@ref	Growth	Type	Temperature (°C)
11668	positive	growth	28
20044	positive	optimum	30
38196	positive	growth	30
67770	positive	growth	28
118933	positive	growth	15-41
118933	negative	growth	5

Physiology and metabolism

Oxygen tolerance

@ref	Oxygen tolerance	Confidence
125439	facultative anaerobe	94.41

Spore formation

@ref	Spore description	Spore formation	Confidence
20044	Formation of spore chains (rectiflixibilis), spore surface warty
125438			90.358

Compound production

11668

Compoundnovobiocin

Halophily

@ref	Salt	Growth	Tested relation	Concentration
20044	NaCl	positive	maximum	2.5 %
118933	NaCl	positive	growth	0-2 %
118933	NaCl		growth	4 %
118933	NaCl		growth	6 %
118933	NaCl		growth	8 %
118933	NaCl		growth	10 %

Observation

@ref	Observation
67770	Hydroxylation of novobiocins
67770	quinones: MK-9(III,VIII-H₄), MK-9(H₂)

Metabolite utilization

@ref	Chebi-ID	Metabolite	Utilization activity	Kind of utilization tested
20044	22599 ChEBI	arabinose	+/-
68368	29016 ChEBI	arginine	+	hydrolysis	from API 20E
20044	62968 ChEBI	cellulose	-
118933	16947 ChEBI	citrate	-	carbon source
68368	16947 ChEBI	citrate	+	assimilation	from API 20E
118933	4853 ChEBI	esculin	-	hydrolysis
20044	28757 ChEBI	fructose	+
68368	5291 ChEBI	gelatin	+	hydrolysis	from API 20E
20044	17234 ChEBI	glucose	+
68368	25094 ChEBI	lysine	-	degradation	from API 20E
20044	29864 ChEBI	mannitol	-
20044	17268 ChEBI	myo-inositol	-
118933	17632 ChEBI	nitrate	-	reduction
118933	17632 ChEBI	nitrate	-	respiration
118933	16301 ChEBI	nitrite	-	reduction
68368	18257 ChEBI	ornithine	+	degradation	from API 20E
20044	16634 ChEBI	raffinose	-
20044	26546 ChEBI	rhamnose	+
20044	17992 ChEBI	sucrose	-
68368	27897 ChEBI	tryptophan	-	energy source	from API 20E
68368	16199 ChEBI	urea	+	hydrolysis	from API 20E
20044	18222 ChEBI	xylose	-

Metabolite production

@ref	Chebi-ID	Metabolite
68368	15688 ChEBI	acetoin	from API 20E
68368	16136 ChEBI	hydrogen sulfide	from API 20E
118933	35581 ChEBI	indole
68368	35581 ChEBI	indole	from API 20E

Metabolite tests

@ref	Chebi-ID	Metabolite	Voges-proskauer-test	Methylred-test	Indole test
118933	15688 ChEBI	acetoin	+
68368	15688 ChEBI	acetoin	+			from API 20E
118933	17234 ChEBI	glucose		-
68368	35581 ChEBI	indole			-	from API 20E

Enzymes

@ref	Value	Activity	Ec
68382	acid phosphatase	+	3.1.3.2	from API zym
118933	alcohol dehydrogenase	-	1.1.1.1
68382	alkaline phosphatase	+	3.1.3.1	from API zym
68382	alpha-fucosidase	-	3.2.1.51	from API zym
118933	amylase	-
68368	arginine dihydrolase	+	3.5.3.6	from API 20E
68382	beta-galactosidase	-	3.2.1.23	from API zym
68368	beta-galactosidase	+	3.2.1.23	from API 20E
68382	beta-glucosidase	-	3.2.1.21	from API zym
68382	beta-glucuronidase	-	3.2.1.31	from API zym
118933	caseinase	-	3.4.21.50
118933	catalase	+	1.11.1.6
68382	cystine arylamidase	-	3.4.11.3	from API zym
118933	DNase	-
68382	esterase lipase (C 8)	-		from API zym
118933	gamma-glutamyltransferase	+	2.3.2.2
118933	gelatinase	+/-
68368	gelatinase	+		from API 20E
68382	lipase (C 14)	-		from API zym
118933	lysine decarboxylase	-	4.1.1.18
68368	lysine decarboxylase	-	4.1.1.18	from API 20E
118933	ornithine decarboxylase	-	4.1.1.17
68368	ornithine decarboxylase	+	4.1.1.17	from API 20E
118933	oxidase	-
118933	phenylalanine ammonia-lyase	-	4.3.1.24
68382	trypsin	-	3.4.21.4	from API zym
118933	tryptophan deaminase	-
68368	tryptophan deaminase	-	4.1.99.1	from API 20E
118933	tween esterase	-
118933	urease	-	3.5.1.5
68368	urease	+	3.5.1.5	from API 20E
68382	valine arylamidase	-		from API zym

API 20E

@ref	ONPG	ADH (Arg)	LDC (Lys)	ODC	CIT	H2S productionH2S	URE	TDA (Trp)	IND	Acetoin production (Voges Proskauer test)VP	GEL	GLU	MAN	INO	Sor	RHA	SAC	MEL	AMY	ARA	OX	Nitrite productionNO2	Reduction to N2N2	MotilityMOB	Growth on MacConkey mediumMAC	OF-O	OF-F
20044	+	+	-	+	+	-	+	-	-	+	+	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.

API zym

@ref	Control	Alkaline phosphatase	Esterase (C 4)	2-naphtyl caprylateEsterase Lipase (C 8)	Lipase (C 14)	L-leucyl-2-naphthylamideLeucine arylamidase	L-valyl-2-naphthylamideValine arylamidase	L-cystyl-2-naphthylamideCystine arylamidase	Trypsin	alpha- Chymotrypsin	Acid phosphatase	Naphthol-AS-BI-phosphateNaphthol-AS-BI-phosphohydrolase	alpha- Galactosidase	beta- Galactosidase	beta- Glucuronidase	alpha- Glucosidase	beta- Glucosidase	N-acetyl-beta- glucosaminidase	alpha- Mannosidase	alpha- Fucosidase
20044	not determinedn.d.	+	-	-	-	-	-	-	-	-	+	-	+	-	-	-	-	-	+	-
118933	-	+	+	-	-	+	-	-	-	+	+	+	-	-	-	+	-	+	-	-

Isolation, sampling and environmental information

Isolation source categories

Cat1	Cat2	Cat3
#Environmental	#Terrestrial	#Soil

Isolation

@ref	Sample type	Isolation date
11668	soil
118933	Environment, Soil	1997

Taxonmaps

69479

Global distribution of 16S sequence AJ294350 (>99% sequence identity) for Nonomuraea from Microbeatlas

Aquatic Samples	3
Soil Samples	53
Animal Samples	9
Plant Samples	22
Total Samples	87

Interaction and safety

Risk assessment

@ref	Biosafety level	Biosafety level comment
11668	1	Risk group (German classification) According to TRBA 466
20044	1	German classification According to TRBA 466
118933	1	Risk group (French classification) According to TRBA 466

Sequence information

Genome sequences

@ref	Description	Assembly level	INSDC accession	BV-BRC accession	IMG accession	NCBI tax ID	Score
66792	ASM1419550v1 assembly for Nonomuraea dietziae DSM 44320	contig	GCA_014195505	65515.3	2827618455	65515	71.03
124043	ASM4266248v1 assembly for Nonomuraea dietziae JCM 3338	contig	GCA_042662485			65515	52.93
124043	ASM4266258v1 assembly for Nonomuraea dietziae JCM 3338	scaffold	GCA_042662585			65515	39.26
124043	ASM4243108v1 assembly for Nonomuraea dietziae JCM 3338	scaffold	GCA_042431085			65515	20.66
124043	ASM3953582v1 assembly for Nonomuraea dietziae JCM 3338	contig	GCA_039535825			65515	0

16S sequences

@ref	Description	Accession	Length	NCBI tax ID
20218	Sebekia benihana 16S rRNA gene for 16S ribosomal RNA, strain DSM 44320	AJ278220	1447	65515
20218	Nonomuraea dietziae gene for 16S ribosomal RNA, partial sequence	AB006156	1473	65515
20218	Sebekia benihana partial 16S rRNA gene, strain NRRL 11111	AJ294350	1450	65515
124043	Nonomuraea dietziae strain JCM 3338 16S ribosomal RNA gene, partial sequence.	MT760452	1313	65515

Genome-based predictions

Predictions

Predictions from Deep Learning for Genome Sequence Data. R package version 0.3.1 based on: ASM1419550v1 assembly for Nonomuraea dietziae DSM 44320
NCBI: GCA_014195505

@ref	Trait	Model	Prediction	Confidence in %	In training data
125439	gram_stain	BacteriaNetⓘ	positive	96.48	no
125439	oxygen_tolerance	BacteriaNetⓘ	facultative anaerobe	94.41	no
125439	motility	BacteriaNetⓘ	no	87.91	no
125439	spore_formation	BacteriaNetⓘ	yes	49.16	no

Predictions from bacterial phenotypic traits through improved machine learning using high-quality, curated datasets based on: Nonomuraea dietziae strain DSM 44320
PATRIC: 65515.3

@ref	Trait	Model	Prediction	Confidence in %	In training data
125438	gram-positive	gram-positiveⓘ	yes	87.98	no
125438	anaerobic	anaerobicⓘ	no	93.35	no
125438	spore-forming	spore-formingⓘ	yes	90.36	yes
125438	aerobic	aerobicⓘ	yes	88.03	no
125438	thermophilic	thermophileⓘ	no	92.09	yes
125438	flagellated	motile2+ⓘ	no	85.00	no

Literature

Topic	Title	Authors	Journal	DOI	Year
Metabolism	Microbial biotransformation products of cyclosporin A.	Kuhnt M, Bitsch F, France J, Hofmann H, Sanglier JJ, Traber R	J Antibiot (Tokyo)	10.7164/antibiotics.49.781	1996
Metabolism	Microbial conversion of nigericin in three successive steps, by Sebekia benihana.	Delort AM, Jeminet G, Sancelme M, Dauphin G	J Antibiot (Tokyo)	10.7164/antibiotics.41.916	1988
Phylogeny	Nonomuraea mangrovi sp. nov., an actinomycete isolated from mangrove soil.	Huang H, Liu M, Zhong W, Mo K, Zhu J, Zou X, Hu Y, Bao S	Int J Syst Evol Microbiol	10.1099/ijsem.0.002954	2018
Phylogeny	Nonomuraea stahlianthi sp. nov., an endophytic actinomycete isolated from the stem of Stahlianthus campanulatus.	Niemhom N, Chutrakul C, Suriyachadkun C, Thawai C	Int J Syst Evol Microbiol	10.1099/ijsem.0.002045	2017
Phylogeny	Nonomuraea rhizophila sp. nov., an actinomycete isolated from rhizosphere soil.	Zhao GZ, Li J, Huang HY, Zhu WY, Xu LH, Li WJ	Int J Syst Evol Microbiol	10.1099/ijs.0.028050-0	2010
Phylogeny	Nonomuraea dietzii sp. nov..	Stackebrandt E, Wink J, Steiner U, Kroppenstedt RM	Int J Syst Evol Microbiol	10.1099/00207713-51-4-1437	2001

References

#11668	Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 44320
#20044	Wink, J.: Compendium of Actinobacteria. HZI-Helmholtz-Centre for Infection Research, Braunschweig .
#20215	Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )
#20216	Curators of the JMRC: Jena Microbial Resource Collection (JMRC):
#20218	Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.: StrainInfo introduces electronic passports for microorganisms.. Syst Appl Microbiol. 37: 42 - 50 2014 ( DOI 10.1016/j.syapm.2013.11.002 , PubMed 24321274 )
#38196	; Curators of the CIP;
#66792	Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .
#67770	Japan Collection of Microorganism (JCM) ; Curators of the JCM;
#68368	Automatically annotated from API 20E .
#68382	Automatically annotated from API zym .
#69479	João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .
#118933	Collection of Institut Pasteur ; Curators of the CIP; CIP 107127
#124043	Isabel Schober, Julia Koblitz: Data extracted from sequence databases, automatically matched based on designation and taxonomy .
#125438	Julia Koblitz, Lorenz Christian Reimer, Rüdiger Pukall, Jörg Overmann: Predicting bacterial phenotypic traits through improved machine learning using high-quality, curated datasets. 2024 ( DOI 10.1101/2024.08.12.607695 )
#125439	Philipp Münch, René Mreches, Martin Binder, Hüseyin Anil Gündüz, Xiao-Yin To, Alice McHardy: deepG: Deep Learning for Genome Sequence Data. R package version 0.3.1 .
#126262	A. Lissin, I. Schober, J. F. Witte, H. Lüken, A. Podstawka, J. Koblitz, B. Bunk, P. Dawyndt, P. Vandamme, P. de Vos, J. Overmann, L. C. Reimer: StrainInfo—the central database for linked microbial strain identifiers. ( DOI 10.1093/database/baaf059 )

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Nonomuraea dietziae (DSM 44320, ATCC 35861, IFO 14309)

Name and taxonomic classification

Morphology

Cell morphology

Colony morphology

Multicellular morphology

Multimedia

Culture and growth conditions

Culture medium

Culture temp

Physiology and metabolism

Oxygen tolerance

Spore formation

Compound production

Halophily

Observation

Metabolite utilization

Metabolite production

Metabolite tests

Enzymes

API 20E

API zym

Isolation, sampling and environmental information

Isolation source categories

Isolation

Taxonmaps

Interaction and safety

Risk assessment

Sequence information

Genome sequences

Genome browser: GCA_014195505

16S sequences

Genome-based predictions

Predictions

Literature

Literature

References

BacDive

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