Name: Chelatococcus sambhunathii HT4
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 1653

Type strain:

Species: Chelatococcus sambhunathii

Strain Designation: HT4

Culture col. no.: DSM 18167, JCM 14988

Strain history: S. K. Das HT4.

NCBI tax ID(s): 363953 (species)

SI-ID 369417

DSM 18167, JCM 14988, LMG 26063

Section

Chelatococcus sambhunathii HT4 is a chemoorganotroph, thermophilic, Gram-negative bacterium that was isolated from sediment of hot sulfur spring.

Gram-negative
motile
rod-shaped
chemoorganotroph
thermophilic
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Pseudomonadota*
	Class	*Alphaproteobacteria*
	Order	*Hyphomicrobiales*
	Family	*Chelatococcaceae*
	Genus	*Chelatococcus*
	Species	**Chelatococcus sambhunathii**
	Full Scientific Name (LPSN)	Chelatococcus sambhunathii Panday and Das 2010

Information on morphological and physiological properties Morphology

[Ref.: #23363]	Gram stain	negative
[Ref.: #69480]	Gram stain	negative Confidence in %99.999

[Ref.: #23363]	Cell length	2.3-2.7 µm

[Ref.: #23363]	Cell width	0.8-1.0 µm

[Ref.: #23363]	Cell shape	rod-shaped

[Ref.: #23363]	Motility	yes

[Ref.: #23363]	Flagellum arrangement	polar

[Ref.: #23363]	Incubation period	3 days
[Ref.: #23363]	Cultivation medium used	TSA

Information on culture and growth conditions Culture and growth conditions

[Ref.: #23363]

Culture medium

Trypticase Soy Agar (TSA)

[Ref.: #23363]

Culture medium growth

		Temperature
[Ref.: #23363]	maximum	50 °C
[Ref.: #23363]	minimum	20 °C
[Ref.: #23363]	optimum	37-42 °C
[Ref.: #67770]	growth	37 °C

[Ref.: #23363]	Temperature range	thermophilic
[Ref.: #23363]	Temperature range	psychrophilic
[Ref.: #67770]	Temperature range	mesophilic

		pH
[Ref.: #23363]	maximum	8.5
[Ref.: #23363]	minimum	6.0
[Ref.: #23363]	optimum	7.5-8.0

Information on physiology and metabolism Physiology and metabolism

[Ref.: #23363]

Nutrition type

chemoorganotroph

[Ref.: #23363]	Ability of spore formation	no
[Ref.: #69481]	Ability of spore formation	no Confidence in %100
[Ref.: #69480]	Ability of spore formation	no Confidence in %99.997

	Halophily	Salt	Tested relation		Salt conc.
[Ref.: #23363]		NaCl		growth	0-3.0	%(w/v)

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #23363]	alanine ChEBI	+	assimilation
[Ref.: #23363]	arabinose ChEBI	+	builds acid from
[Ref.: #23363]	arginine ChEBI	-	assimilation
[Ref.: #23363]	aspartate ChEBI	+	assimilation
[Ref.: #23363]	cellobiose ChEBI	-	carbon source
[Ref.: #23363]	citrate ChEBI	+	assimilation
[Ref.: #23363]	cysteine ChEBI	-	carbon source
[Ref.: #23363]	D-arabinose ChEBI	-	carbon source
[Ref.: #23363]	esculin ChEBI	-	carbon source
[Ref.: #23363]	galactose ChEBI	-	carbon source
[Ref.: #23363]	gluconate ChEBI	-	carbon source
[Ref.: #23363]	glucose ChEBI	-	fermentation
[Ref.: #23363]	glucose ChEBI	+	builds acid from
[Ref.: #23363]	glutamine ChEBI	+	assimilation
[Ref.: #23363]	glycerol ChEBI	-	carbon source
[Ref.: #23363]	glycine ChEBI	+	carbon source
[Ref.: #23363]	histidine ChEBI	+	assimilation
[Ref.: #23363]	hydrogen sulfide ChEBI	+	other
[Ref.: #23363]	isoleucine ChEBI	+	assimilation
[Ref.: #23363]	L-asparagine ChEBI	+	assimilation
[Ref.: #23363]	L-glutamate ChEBI	+	assimilation
[Ref.: #23363]	lactose ChEBI	-	assimilation
[Ref.: #23363]	leucine ChEBI	+	assimilation
[Ref.: #23363]	lysine ChEBI	-	carbon source
[Ref.: #23363]	maltose ChEBI	-	assimilation
[Ref.: #23363]	mannitol ChEBI	-	assimilation
[Ref.: #23363]	mannose ChEBI	-	carbon source
[Ref.: #23363]	methionine ChEBI	-	carbon source
[Ref.: #23363]	myo-inositol ChEBI	-	carbon source
[Ref.: #23363]	myo-inositol ChEBI	+	builds acid from
[Ref.: #23363]	oxalate ChEBI	-	assimilation
[Ref.: #23363]	phenylalanine ChEBI	-	assimilation
[Ref.: #23363]	proline ChEBI	+	assimilation
[Ref.: #23363]	raffinose ChEBI	-	assimilation
[Ref.: #23363]	rhamnose ChEBI	-	carbon source
[Ref.: #23363]	rhamnose ChEBI	+	builds acid from
[Ref.: #23363]	ribitol ChEBI	-	carbon source
[Ref.: #23363]	salicin ChEBI	-	carbon source
[Ref.: #23363]	serine ChEBI	+	assimilation
[Ref.: #23363]	sorbitol ChEBI	-	assimilation
[Ref.: #23363]	sorbose ChEBI	-	assimilation
[Ref.: #23363]	starch ChEBI	+	hydrolysis
[Ref.: #23363]	sucrose ChEBI	-	assimilation
[Ref.: #23363]	threonine ChEBI	-	carbon source
[Ref.: #23363]	tryptophan ChEBI	-	assimilation
[Ref.: #23363]	tryptophan ChEBI	+	energy source
[Ref.: #23363]	tyrosine ChEBI	-	carbon source
[Ref.: #23363]	valine ChEBI	+	assimilation
[Ref.: #23363]	xylitol ChEBI	-	carbon source
[Ref.: #23363]	xylose ChEBI	-	carbon source
	Only first 10 entries are displayed. Click here to see all.

	Metabolite	Antibiotic sensitivity	Concentration		Antibiotic resistance	Concentration
[Ref.: #23363]	ampicillin ChEBI	yes	40	µg/mL
[Ref.: #23363]	chloramphenicol ChEBI	yes	30	µg/mL
[Ref.: #23363]	kanamycin ChEBI				yes	50	µg/mL
[Ref.: #23363]	nalidixic acid ChEBI	yes	20	µg/mL
[Ref.: #23363]	neomycin ChEBI				yes	30	µg/mL
[Ref.: #23363]	rifampicin ChEBI	yes	20	µg/mL
[Ref.: #23363]	streptomycin ChEBI	yes	25	µg/mL
[Ref.: #23363]	tetracycline ChEBI	yes	15	µg/mL

	Metabolite production	Metabolite	Production
[Ref.: #23363]		acetoin ChEBI	no
[Ref.: #23363]		indole ChEBI	yes

	Metabolite	Voges-Proskauer-test	Methylred-test	Indole test
[Ref.: #23363]	acetoin ChEBI	-
[Ref.: #23363]	glucose ChEBI		-
[Ref.: #23363]	indole ChEBI			+

	Enzyme	Enzyme activity	EC number
[Ref.: #23363]	arginine dihydrolase	+	3.5.3.6
[Ref.: #23363]	catalase	+	1.11.1.6
[Ref.: #23363]	cytochrome oxidase	+	1.9.3.1
[Ref.: #23363]	tryptophan deaminase	+	4.1.99.1
[Ref.: #23363]	urease	+	3.5.1.5

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #7341]	Sample type/isolated from	sediment of hot sulfur spring
[Ref.: #7341]	Geographic location (country and/or sea, region)	Orissa, Athamallik
[Ref.: #7341]	Country	India
[Ref.: #7341]	Country ISO 3 Code	IND
[Ref.: #7341]	Continent	Asia

[Ref.: #67770]	Sample type/isolated from	Hot spring
[Ref.: #67770]	Country	India
[Ref.: #67770]	Country ISO 3 Code	IND
[Ref.: #67770]	Continent	Asia
* marker position based on {}

Isolation sources categories

#Environmental	#Aquatic	#Sediment
#Environmental	#Aquatic	#Thermal spring
#Condition	#Sulfuric	-
#Condition	#Thermophilic (>45°C)	-

What are isolation sources categories?

[Ref.: #69479]

Global distribution of 16S sequence DQ322070 (>99% sequence identity) for Chelatococcus from Microbeatlas

Aquatic Samples	127
Soil Samples	286
Animal Samples	207
Plant Samples	60
Total Samples	680

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #7341]

Biosafety level

Risk group (German classification)
According to TRBA 466

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #7341]	Chelatococcus sambhunathii strain HT4 16S ribosomal RNA gene, partial sequence	DQ322070	1378	ENA	363953 tax ID

Genome sequence information:

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #67770]	Chelatococcus sambhunathii DSM 18167	GCA_001517345	scaffold	GenBank	363953 tax ID
[Ref.: #67770]	Chelatococcus sambhunathii DSM 18167	GCA_001418005	scaffold	GenBank	363953 tax ID
[Ref.: #66792]	Chelatococcus sambhunathii strain DSM 18167	363953.4	wgs	PATRIC	363953 tax ID	*
[Ref.: #66792]	Chelatococcus sambhunathii DSM 18167	2615840613	draft	JGI IMG/M	363953 tax ID	*

[Ref.: #7341]

GC-content

67.8 mol%

high performance liquid chromatography (HPLC)

Data predicted using genome information as a basis Genome-based predictions

	Trait	Prediction	Confidence in %	In training data
	Predictions from GenomeNet Sporulation v. 1 based on: Chelatococcus sambhunathii DSM 18167 NCBI: GCA_001418005.1
[Ref.: #69481]	spore-forming	no	100	no

	Trait	Prediction	Confidence in %	In training data
	Predictions from Diaspora project based on: Chelatococcus sambhunathii strain DSM 18167 PATRIC: 363953.4
[Ref.: #69480]	gram-positive	no	98.586	yes
[Ref.: #69480]	anaerobic	no	97.763	no
[Ref.: #69480]	halophile	no	88.88	no
[Ref.: #69480]	spore-forming	no	94.29	no
[Ref.: #69480]	glucose-util	yes	87.81	no
[Ref.: #69480]	aerobic	yes	87.255	no
[Ref.: #69480]	motile	yes	87.989	yes
[Ref.: #69480]	flagellated	yes	53.892	yes
[Ref.: #69480]	thermophile	no	88.667	no
[Ref.: #69480]	glucose-ferment	no	91.341	no

Availability in culture collections External links

[Ref.: #7341]

Culture collection no.

DSM 18167, JCM 14988

[Ref.: #71300]

SI-ID 369417

Literature:

Topic	Title	Authors	Journal	DOI	Year
Genetics	Draft Genome Sequence of Chelatococcus sambhunathii Strain HT4T (DSM 18167T) Isolated from a Hot Spring in India.	Badhai J, Whitman WB, Das SK	Genome Announc	10.1128/genomeA.00825-16	2016	*
Phylogeny	Chelatococcus sambhunathii sp. nov., a moderately thermophilic alphaproteobacterium isolated from hot spring sediment.	Panday D, Das SK	Int J Syst Evol Microbiol	10.1099/ijs.0.013466-0	2009	*

References

#7341

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 18167

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#23363

Digvijay Panday, Subrata K. Das: Chelatococcus sambhunathii sp. nov., a moderately thermophilic alphaproteobacterium isolated from hot spring sediment. IJSEM 60: 861 - 865 2010 ( DOI 10.1099/ijs.0.013466-0 , PubMed 19661510 )

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#67770 Japan Collection of Microorganism (JCM) ; Curators of the JCM;

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

#69480

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#69481

Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch: Predictions based on the model GenomeNet Sporulation v. 1 . ( DOI 10.21203/rs.3.rs-2527258/v1 )

#71300

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID369417.1 )

* These data were automatically processed and therefore are not curated

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Chelatococcus sambhunathii DSM 18167

Chelatococcus sambhunathii strain DSM 18167

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