Name: Nonomuraea phyllanthi PA1-10
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 164452

Type strain:

Species: Nonomuraea phyllanthi

Strain Designation: PA1-10

Culture col. no.: JCM 33073, NBRC 112774, TISTR 2497

Strain history: S. Tanasupawat; Chulalongkorn Univ., Thailand; PA1-10.

NCBI tax ID(s): 2219224 (species)

Section

Nonomuraea phyllanthi PA1-10 is an aerobe, spore-forming, mesophilic bacterium that was isolated from Stem of Phyllanthus amarus from a botanical garden of the Faculty of Pharmaceutical Sciences.

filament-shaped
Gram-positive
spore-forming
aerobe
mesophilic
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Actinomycetota*
	Class	*Actinomycetes*
	Order	*Streptosporangiales*
	Family	*Streptosporangiaceae*
	Genus	*Nonomuraea*
	Species	**Nonomuraea phyllanthi**
	Full Scientific Name (LPSN)	Nonomuraea phyllanthi Klykleung et al. 2020

Synonym

Nonomuraea nitratireducens

Information on morphological and physiological properties Morphology

[Ref.: #68254]	Gram stain	positive

[Ref.: #68254]	Cell shape	filament-shaped

[Ref.: #68254]	Motility	no

[Ref.: #68254]	Multicellular complex forming ability	yes
[Ref.: #68254]	Multicellular complex color	forms abundant white aerial mycelia and yellow to brown vegetative mycelia
[Ref.: #68254]	Medium Name (multicellularity)	ISP2, ISP3, ISP4, ISP5, ISP6, ISP7, nutrient agar

Information on culture and growth conditions Culture and growth conditions

		Temperature
[Ref.: #67770]	growth	28 °C
[Ref.: #68254]	growth	15-40 °C
[Ref.: #68254]	optimum	25-37 °C

[Ref.: #67770]	Temperature range	mesophilic
[Ref.: #68254]	Temperature range	mesophilic

	pH	Kind of pH		pH
[Ref.: #68254]			optimum	7
[Ref.: #68254]			growth	5-9

Information on physiology and metabolism Physiology and metabolism

[Ref.: #68254]

Oxygen tolerance

aerobe

[Ref.: #68254]

Ability of spore formation

yes

	Halophily	Salt	Tested relation		Salt conc.
[Ref.: #68254]		NaCl		growth	0-4	%(w/v)

[Ref.: #67770]	Observation	quinones: MK-9(H₂), MK-9(H₄), MK-9(H₆)
[Ref.: #68254]	Observation	formes the spiral short chains of the rugose spores and pseudosporangia on aerial mycelium

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #68254]	cellobiose ChEBI	-	assimilation
[Ref.: #68254]	cellulose ChEBI	-	assimilation
[Ref.: #68254]	D-glucose ChEBI	+	assimilation
[Ref.: #68254]	D-mannitol ChEBI	-	assimilation
[Ref.: #68254]	D-mannose ChEBI	+	assimilation
[Ref.: #68254]	fructose ChEBI	-	assimilation
[Ref.: #68254]	gelatin ChEBI	-	hydrolysis
[Ref.: #68254]	glycerol ChEBI	-	assimilation
[Ref.: #68254]	L-arabinose ChEBI	-	assimilation
[Ref.: #68254]	L-rhamnose ChEBI	-	assimilation
[Ref.: #68254]	melibiose ChEBI	+	assimilation
[Ref.: #68254]	myo-inositol ChEBI	+	assimilation
[Ref.: #68254]	nitrate ChEBI	+	reduction
[Ref.: #68254]	raffinose ChEBI	+	assimilation
[Ref.: #68254]	skimmed milk	-	assimilation
[Ref.: #68254]	starch ChEBI	-	hydrolysis
[Ref.: #68254]	sucrose ChEBI	+	assimilation
[Ref.: #68254]	xylose ChEBI	-	assimilation
	Only first 10 entries are displayed. Click here to see all.

	Enzyme	Enzyme activity	EC number
[Ref.: #68254]	acid phosphatase	+/-	3.1.3.2
[Ref.: #68254]	alkaline phosphatase	+	3.1.3.1
[Ref.: #68254]	alpha-chymotrypsin	+	3.4.21.1
[Ref.: #68254]	alpha-fucosidase	-	3.2.1.51
[Ref.: #68254]	alpha-galactosidase	+/-	3.2.1.22
[Ref.: #68254]	alpha-glucosidase	+	3.2.1.20
[Ref.: #68254]	alpha-mannosidase	+/-	3.2.1.24
[Ref.: #68254]	beta-galactosidase	+	3.2.1.23
[Ref.: #68254]	beta-glucosidase	+/-	3.2.1.21
[Ref.: #68254]	beta-glucuronidase	-	3.2.1.31
[Ref.: #68254]	cystine arylamidase	+/-	3.4.11.3
[Ref.: #68254]	esterase (C 4)	+/-
[Ref.: #68254]	esterase Lipase (C 8)	+/-
[Ref.: #68254]	leucine arylamidase	+	3.4.11.1
[Ref.: #68254]	lipase (C 14)	-
[Ref.: #68254]	N-acetyl-beta-glucosaminidase	+	3.2.1.52
[Ref.: #68254]	naphthol-AS-BI-phosphohydrolase	+
[Ref.: #68254]	trypsin	+/-	3.4.21.4
[Ref.: #68254]	valine arylamidase	+
	Only first 10 entries are displayed. Click here to see all.

Fatty acid profile

Metadata FA analysis

Reference

[Ref.: #68254]

Fatty acid	Percentage
C16:0 iso	32.2
C17:0 10-methyl	11.2
C16:0	8.3
C15:0 iso	6.9
C17:0	5.6
C17:1 w8c	5.4
C17:0 anteiso	5
C16:0 10-methyl	4.4
C16:1 w7c/C16:1 w6c	3.1
C17:0 iso	2.5
C16:1 iso H	2.2
C15:0 anteiso	2.1
C18:0	1.9
C18:1 w9c	1.8
C18:0 10-methyl	1.4
C18:0 iso	1.4
C14:0	1.2
C15:0 2OH	0.7
C14:0 iso	0.7
C16:0 2OH	0.6
C17:1 anteiso w9c	0.5

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #67770]	Sample type/isolated from	Stem of Phyllanthus amarus from a botanical garden of the Faculty of Pharmaceutical Sciences
[Ref.: #67770]	Host species	Phyllanthus amarus
[Ref.: #67770]	Geographic location (country and/or sea, region)	Chulalongkorn University, Bangkok
[Ref.: #67770]	Country	Thailand
[Ref.: #67770]	Country ISO 3 Code	THA
[Ref.: #67770]	Continent	Asia

[Ref.: #68254]	Sample type/isolated from	stem of Phyllanthus amarus
[Ref.: #68254]	Geographic location (country and/or sea, region)	botanical garden of the Faculty of Pharmaceutical Sciences, Chulalongkorn University, Bangkok
[Ref.: #68254]	Country	Thailand
[Ref.: #68254]	Country ISO 3 Code	THA
[Ref.: #68254]	Continent	Asia
[Ref.: #68254]	Enrichment culture	starch casein nitrate medium
[Ref.: #68254]	Enrichment culture duration	3-4 weeks
[Ref.: #68254]	Enrichment culture temperature	30 ̊C
* marker position based on {}

[Ref.: #69479]

Global distribution of 16S sequence LC387602 (>99% sequence identity) for Nonomuraea from Microbeatlas

Aquatic Samples
Soil Samples	72
Animal Samples	4
Plant Samples	15
Total Samples	91

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #68254]	Nonomuraea phyllanthi PA1-10 gene for 16S ribosomal RNA, partial sequence	LC387602	1459	ENA	2219224 tax ID

Genome sequence information:

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #67770]	Nonomuraea phyllanthi PA1-10	GCA_006334985	contig	GenBank	2219224 tax ID
[Ref.: #66792]	Nonomuraea phyllanthi PA1-10	2895442618	draft	JGI IMG/M	2219224 tax ID	*

[Ref.: #68254]	GC-content	72 mol%	high performance liquid chromatography (HPLC)
[Ref.: #67770]	GC-content	71.2 mol%	genome sequence analysis

Availability in culture collections External links

[Ref.: #67770]

Culture collection no.

JCM 33073, NBRC 112774, TISTR 2497

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Nonomuraea nitratireducens Qu et al. 2020 is a later heterotypic synonym of Nonomuraea phyllanthi Klykleung et al. 2020.	Wu Y, Peng M, Gao Y, Pei J, Zhang Y, Li S, Fang J, Wei Y	Int J Syst Evol Microbiol	10.1099/ijsem.0.005013	2021	*
Phylogeny	Nonomuraea phyllanthi sp. nov., an endophytic actinomycete isolated from the leaf of Phyllanthus amarus.	Klykleung N, Yuki M, Kudo T, Ohkuma M, Phongsopitanun W, Pittayakhajonwut P, Tanasupawat S	Arch Microbiol	10.1007/s00203-019-01717-w	2019	*

References

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#67770 Japan Collection of Microorganism (JCM) ; Curators of the JCM;

#68254

Nattaporn Klykleung, Masahiro Yuki, Takuji Kudo, Moriya Ohkuma, Wongsakorn Phongsopitanun, Pattama Pittayakhajonwut and Somboon Tanasupawat: Nonomuraea phyllanthi sp. nov., an endophytic actinomycete isolated from the leaf of Phyllanthus amarus. Arch Microbiol 202: 55 - 61 2020 ( DOI 10.1007/s00203-019-01717-w )

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

* These data were automatically processed and therefore are not curated

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