Name: Methylobacterium segetis 17J42-1
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 164450

Type strain:

Species: Methylobacterium segetis

Strain Designation: 17J42-1

Culture col. no.: JCM 33059, KCTC 62267

Strain history: H.-Y. Jung and M. K. Kim; Kyungpook Natl. Univ., South Korea; 17J42-1.

NCBI tax ID(s): 2488750 (species)

Section

Methylobacterium segetis 17J42-1 is an aerobe, methylotroph, mesophilic bacterium that forms circular colonies and was isolated from Soil on Jeju Island.

colony-forming
Gram-negative
motile
rod-shaped
aerobe
mesophilic
methylotroph
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Pseudomonadota*
	Class	*Alphaproteobacteria*
	Order	*Hyphomicrobiales*
	Family	*Methylobacteriaceae*
	Genus	*Methylobacterium*
	Species	**Methylobacterium segetis**
	Full Scientific Name (LPSN)	Methylobacterium segetis Ten et al. 2020

Information on morphological and physiological properties Morphology

[Ref.: #68265]	Gram stain	negative
[Ref.: #69480]	Gram stain	negative Confidence in %100

[Ref.: #68265]	Cell length	2.3-3.7 µm

[Ref.: #68265]	Cell width	1.2-1.4 µm

[Ref.: #68265]	Cell shape	rod-shaped

[Ref.: #68265]	Motility	yes
[Ref.: #69480]	Motility	yes Confidence in %92.822

[Ref.: #68265]	Flagellum arrangement	gliding

[Ref.: #68265]	Colony color	pink
[Ref.: #68265]	Colony shape	circular
[Ref.: #68265]	Cultivation medium used	Reasoner's 2A agar (R2A)

Information on culture and growth conditions Culture and growth conditions

	Temperatures	Kind of temperature		Temperature
[Ref.: #67770]			growth	25 °C
[Ref.: #68265]			growth	15-35 °C

[Ref.: #67770]

Temperature range

mesophilic

	pH	Kind of pH		pH
[Ref.: #68265]			growth	5-9

Information on physiology and metabolism Physiology and metabolism

[Ref.: #68265]

Oxygen tolerance

aerobe

[Ref.: #68265]

Nutrition type

methylotroph

[Ref.: #69480]	Ability of spore formation	no Confidence in %99.991
[Ref.: #69481]	Ability of spore formation	no Confidence in %100

	Halophily	Salt	Tested relation		Salt conc.
[Ref.: #68265]		NaCl		growth	1	%(w/v)
[Ref.: #68265]		NaCl	no	growth	2	%(w/v)

[Ref.: #67770]

Observation

quinones: Q-10

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #68265]	3-hydroxybenzoate ChEBI	-	assimilation
[Ref.: #68265]	3-hydroxybutyrate ChEBI	+	assimilation
[Ref.: #68265]	4-hydroxybenzoate ChEBI	-	assimilation
[Ref.: #68265]	adipate ChEBI	-	assimilation
[Ref.: #68265]	D-glucose ChEBI	-	assimilation
[Ref.: #68265]	D-mannitol ChEBI	-	assimilation
[Ref.: #68265]	D-mannose ChEBI	-	assimilation
[Ref.: #68265]	D-ribose ChEBI	-	assimilation
[Ref.: #68265]	D-sorbitol ChEBI	-	assimilation
[Ref.: #68265]	decanoate ChEBI	-	assimilation
[Ref.: #68265]	esculin ChEBI	-	hydrolysis
[Ref.: #68265]	gelatin ChEBI	+	hydrolysis
[Ref.: #68265]	glucose ChEBI	-	builds acid from
[Ref.: #68265]	glycogen ChEBI	-	assimilation
[Ref.: #68265]	itaconate ChEBI	-	assimilation
[Ref.: #68265]	L-alanine ChEBI	-	assimilation
[Ref.: #68265]	L-arabinose ChEBI	-	assimilation
[Ref.: #68265]	L-fucose ChEBI	-	assimilation
[Ref.: #68265]	L-histidine ChEBI	-	assimilation
[Ref.: #68265]	L-proline ChEBI	-	assimilation
[Ref.: #68265]	L-rhamnose ChEBI	-	assimilation
[Ref.: #68265]	L-serine ChEBI	-	assimilation
[Ref.: #68265]	lactate ChEBI	+	assimilation
[Ref.: #68265]	malate ChEBI	-	assimilation
[Ref.: #68265]	maltose ChEBI	-	assimilation
[Ref.: #68265]	melibiose ChEBI	-	assimilation
[Ref.: #68265]	methanol ChEBI	+	carbon source
[Ref.: #68265]	methanol ChEBI	+	energy source
[Ref.: #68265]	myo-inositol ChEBI	-	assimilation
[Ref.: #68265]	N-acetylglucosamine ChEBI	-	assimilation
[Ref.: #68265]	nitrate ChEBI	-	reduction
[Ref.: #68265]	phenylacetate ChEBI	-	assimilation
[Ref.: #68265]	potassium 2-dehydro-D-gluconate	-	assimilation
[Ref.: #68265]	potassium 5-dehydro-D-gluconate	-	assimilation
[Ref.: #68265]	potassium gluconate ChEBI	-	assimilation
[Ref.: #68265]	propionate ChEBI	-	assimilation
[Ref.: #68265]	salicin ChEBI	-	assimilation
[Ref.: #68265]	sodium acetate ChEBI	-	assimilation
[Ref.: #68265]	sodium citrate ChEBI	-	assimilation
[Ref.: #68265]	sodium malonate ChEBI	-	assimilation
[Ref.: #68265]	suberate ChEBI	-	assimilation
[Ref.: #68265]	sucrose ChEBI	-	assimilation
[Ref.: #68265]	urea ChEBI	+	hydrolysis
[Ref.: #68265]	valerate ChEBI	-	assimilation
	Only first 10 entries are displayed. Click here to see all.

	Metabolite production	Metabolite	Production
[Ref.: #68265]		indole ChEBI	no

	Enzyme	Enzyme activity	EC number
[Ref.: #68265]	acid phosphatase	+	3.1.3.2
[Ref.: #68265]	alkaline phosphatase	-	3.1.3.1
[Ref.: #68265]	alpha-galactosidase	-	3.2.1.22
[Ref.: #68265]	alpha-glucosidase	-	3.2.1.20
[Ref.: #68265]	alpha-mannosidase	-	3.2.1.24
[Ref.: #68265]	arginine dihydrolase	-	3.5.3.6
[Ref.: #68265]	beta-D-fucosidase	-	3.2.1.38
[Ref.: #68265]	beta-galactosidase	-	3.2.1.23
[Ref.: #68265]	beta-glucosidase	-	3.2.1.21
[Ref.: #68265]	beta-glucuronidase	-	3.2.1.31
[Ref.: #68265]	catalase	+	1.11.1.6
[Ref.: #68265]	chymotrypsin	-	3.4.4.5
[Ref.: #68265]	cystine arylamidase	-	3.4.11.3
[Ref.: #68265]	cytochrome oxidase	+	1.9.3.1
[Ref.: #68265]	esterase (C 4)	+
[Ref.: #68265]	esterase Lipase (C 8)	+
[Ref.: #68265]	leucine arylamidase	+	3.4.11.1
[Ref.: #68265]	lipase (C 14)	-
[Ref.: #68265]	N-acetyl-beta-glucosaminidase	-	3.2.1.52
[Ref.: #68265]	naphthol-AS-BI-phosphohydrolase	+
[Ref.: #68265]	trypsin	+/-	3.4.21.4
[Ref.: #68265]	valine arylamidase	-
	Only first 10 entries are displayed. Click here to see all.

Fatty acid profile

Metadata FA analysis

Reference

[Ref.: #68265]

Fatty acid	Percentage
C18:1 w7c	85.3
C19:0 cyclo w8c	4.5
C18:0	3.5
C16:0	2.6
C18:0 3OH	1.8
C14:0 3OH/iso-C16:1 I	1.6
C16:1 w7c/C16:1 w6c/iso-C15:0 2OH	0.5

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #67770]	Sample type/isolated from	Soil on Jeju Island
[Ref.: #67770]	Country	Republic of Korea
[Ref.: #67770]	Country ISO 3 Code	KOR
[Ref.: #67770]	Continent	Asia

[Ref.: #68265]	Sample type/isolated from	soil
[Ref.: #68265]	Geographic location (country and/or sea, region)	Jeju Island
[Ref.: #68265]	Country	Republic of Korea
[Ref.: #68265]	Country ISO 3 Code	KOR
[Ref.: #68265]	Continent	Asia
[Ref.: #68265]	Geographic location	33.3259°/126.8420°
[Ref.: #68265]	Enrichment culture	R2A
[Ref.: #68265]	Enrichment culture temperature	25 ̊C
* marker position based on {}

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #67770]	Methylobacterium sp. 17J42-1 gene for 16S ribosomal RNA, partial sequence	LC433921	1417	ENA	2488750 tax ID

Genome sequence information:

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #68265]	Methylobacterium segetis 17J42-1	GCA_004348265	contig	GenBank	2488750 tax ID
[Ref.: #66792]	Methylobacterium sp. 17J42-1	2488750.6	wgs	PATRIC	2488750 tax ID	*

[Ref.: #68265]

GC-content

70.2 mol%

genome sequence analysis

Data predicted using genome information as a basis Genome-based predictions

	Trait	Prediction	Confidence in %	In training data
	Predictions from GenomeNet Sporulation v. 1 based on: Methylobacterium sp. 17J42-1 PATRIC: 2488750.6
[Ref.: #69481]	spore-forming	no	100	no

	Trait	Prediction	Confidence in %	In training data
	Predictions from Diaspora project based on: Methylobacterium sp. 17J42-1 PATRIC: 2488750.6
[Ref.: #69480]	motile	yes	86.763	no
[Ref.: #69480]	gram-positive	no	97.717	yes
[Ref.: #69480]	anaerobic	no	97.641	yes
[Ref.: #69480]	aerobic	yes	86.859	no
[Ref.: #69480]	halophile	no	92.62	yes
[Ref.: #69480]	spore-forming	no	92.235	no
[Ref.: #69480]	glucose-util	yes	80.028	yes
[Ref.: #69480]	flagellated	no	78.83	yes
[Ref.: #69480]	thermophile	no	98.954	yes
[Ref.: #69480]	glucose-ferment	no	90.258	no

Availability in culture collections External links

[Ref.: #67770]

Culture collection no.

JCM 33059, KCTC 62267

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Methylobacterium segetis sp. nov., a novel member of the family Methylobacteriaceae isolated from soil on Jeju Island.	Ten LN, Li W, Elderiny NS, Kim MK, Lee SY, Rooney AP, Jung HY	Arch Microbiol	10.1007/s00203-019-01784-z	2019	*

References

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#67770 Japan Collection of Microorganism (JCM) ; Curators of the JCM;

#68265

Leonid N. Ten, Weilan Li, Nabil Salah Elderiny, Myung Kyum Kim, Seung-Yeol Lee, Alejandro P. Rooney & Hee-Young Jung: Methylobacterium segetis sp. nov., a novel member of the family Methylobacteriaceae isolated from soil on Jeju Island. Arch Microbiol 202: 747 - 754 2020 ( DOI 10.1007/s00203-019-01784-z )

#69480

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#69481

Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch: Predictions based on the model GenomeNet Sporulation v. 1 . ( DOI 10.21203/rs.3.rs-2527258/v1 )

* These data were automatically processed and therefore are not curated

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Methylobacterium sp. 17J42-1

Methylobacterium sp. 17J42-1

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