Name: Lentibacillus lipolyticus SSKP1-9
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 164308

Type strain:

Species: Lentibacillus lipolyticus

Strain Designation: SSKP1-9

Culture col. no.: JCM 32625, TISTR 2597

Strain history: S. Tanasupawat; Chulalongkorn Univ., Thailand; SSKP1-9.

NCBI tax ID(s): 2510307 (species)

Section

Lentibacillus lipolyticus SSKP1-9 is an aerobe, moderately halophilic, spore-forming bacterium that was isolated from Ka-pi, a traditional salted shrimp paste of Thailand.

Gram-positive
motile
rod-shaped
spore-forming
aerobe
mesophilic
moderately halophilic
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Bacillota*
	Class	*Bacilli*
	Order	*Caryophanales*
	Family	*Bacillaceae*
	Genus	*Lentibacillus*
	Species	**Lentibacillus lipolyticus**
	Full Scientific Name (LPSN)	Lentibacillus lipolyticus Booncharoen et al. 2019

Information on morphological and physiological properties Morphology

[Ref.: #68217]	Gram stain	positive
[Ref.: #69480]	Gram stain	positive Confidence in %100

[Ref.: #68217]	Cell length	1.6-2.7 µm

[Ref.: #68217]	Cell width	0.4-0.6 µm

[Ref.: #68217]	Cell shape	rod-shaped

[Ref.: #68217]	Motility	yes
[Ref.: #69480]	Motility	yes Confidence in %92.821

[Ref.: #68217]	Flagellum arrangement	peritrichous

Information on culture and growth conditions Culture and growth conditions

		Temperature
[Ref.: #67770]	growth	35 °C
[Ref.: #68217]	growth	20-50 °C
[Ref.: #68217]	optimum	37-40 °C

[Ref.: #67770]

Temperature range

mesophilic

	pH	Kind of pH		pH
[Ref.: #68217]			optimum	7
[Ref.: #68217]			growth	5-8

Information on physiology and metabolism Physiology and metabolism

[Ref.: #68217]

Oxygen tolerance

aerobe

[Ref.: #68217]	Ability of spore formation	yes
[Ref.: #69480]	Ability of spore formation	yes Confidence in %99.999
[Ref.: #69481]	Ability of spore formation	yes Confidence in %100

[Ref.: #68217]

Halophily / tolerance level

moderately halophilic

	Halophily	Salt	Tested relation		Salt conc.
[Ref.: #68217]		NaCl		growth	4-35	%(w/v)
[Ref.: #68217]		NaCl		optimum	8-16	%(w/v)

[Ref.: #67770]	Observation	quinones: MK-7
[Ref.: #68217]	Observation	formes oval terminal endospores in swollen sporangia

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #68217]	adipate ChEBI	-	assimilation
[Ref.: #68217]	D-glucose ChEBI	+	builds acid from
[Ref.: #68217]	D-glucose ChEBI	-	assimilation
[Ref.: #68217]	D-mannitol ChEBI	-	assimilation
[Ref.: #68217]	D-mannose ChEBI	-	assimilation
[Ref.: #68217]	D-ribose ChEBI	+	builds acid from
[Ref.: #68217]	D-tagatose ChEBI	+	builds acid from
[Ref.: #68217]	esculin ChEBI	+	builds acid from
[Ref.: #68217]	gelatin ChEBI	+	hydrolysis
[Ref.: #68217]	glucose ChEBI	-	fermentation
[Ref.: #68217]	glycerol ChEBI	+	builds acid from
[Ref.: #68217]	hexanoate ChEBI	-	assimilation
[Ref.: #68217]	L-arabinose ChEBI	-	assimilation
[Ref.: #68217]	lecithin ChEBI	-	hydrolysis
[Ref.: #68217]	malate ChEBI	-	assimilation
[Ref.: #68217]	maltose ChEBI	-	assimilation
[Ref.: #68217]	N-acetyl-D-glucosamine ChEBI	-	assimilation
[Ref.: #68217]	N-acetylglucosamine ChEBI	+	builds acid from
[Ref.: #68217]	nitrate ChEBI	+	reduction
[Ref.: #68217]	phenylacetate ChEBI	-	assimilation
[Ref.: #68217]	potassium 5-dehydro-D-gluconate	+	builds acid from
[Ref.: #68217]	potassium gluconate ChEBI	-	assimilation
[Ref.: #68217]	sodium citrate ChEBI	-	assimilation
[Ref.: #68217]	starch ChEBI	-	hydrolysis
[Ref.: #68217]	tributyrin ChEBI	-	hydrolysis
[Ref.: #68217]	tween 20 ChEBI	-	hydrolysis
[Ref.: #68217]	tween 40 ChEBI	+	hydrolysis
[Ref.: #68217]	tween 60 ChEBI	+	hydrolysis
[Ref.: #68217]	tween 80 ChEBI	-	hydrolysis
	Only first 10 entries are displayed. Click here to see all.

	Metabolite	Antibiotic sensitivity	Concentration		Antibiotic resistance	Concentration
[Ref.: #68217]	amikacin ChEBI	yes	30	µg (disc)
[Ref.: #68217]	amoxicillin ChEBI	yes	30	µg (disc)
[Ref.: #68217]	ampicillin ChEBI	yes	10	µg (disc)
[Ref.: #68217]	bacitracin ChEBI	yes	10	Unit (disc)
[Ref.: #68217]	cefepime ChEBI	yes	30	µg (disc)
[Ref.: #68217]	cefotaxime ChEBI	yes	30	µg (disc)
[Ref.: #68217]	cefoxitin ChEBI	yes	30	µg (disc)
[Ref.: #68217]	ceftazidime ChEBI	yes	30	µg (disc)
[Ref.: #68217]	ceftriaxone ChEBI	yes	30	µg (disc)
[Ref.: #68217]	chloramphenicol ChEBI	yes	30	µg (disc)
[Ref.: #68217]	clindamycin ChEBI	yes	2	µg (disc)
[Ref.: #68217]	ertapenem ChEBI	yes	10	µg (disc)
[Ref.: #68217]	erythromycin ChEBI	yes	15	µg (disc)
[Ref.: #68217]	gentamicin ChEBI	yes	10	µg (disc)
[Ref.: #68217]	imipenem ChEBI	yes	10	µg (disc)
[Ref.: #68217]	kanamycin ChEBI	yes	30	µg (disc)
[Ref.: #68217]	levofloxacin ChEBI	yes	5	µg (disc)
[Ref.: #68217]	meropenem ChEBI	yes	10	µg (disc)
[Ref.: #68217]	nalidixic acid ChEBI				yes	30	µg (disc)
[Ref.: #68217]	neomycin ChEBI				yes	30	µg (disc)
[Ref.: #68217]	netilmicin ChEBI	yes	30	µg (disc)
[Ref.: #68217]	nitrofurantoin ChEBI	yes	300	µg (disc)
[Ref.: #68217]	novobiocin ChEBI	yes	30	µg (disc)
[Ref.: #68217]	nystatin ChEBI	yes	10	µg (disc)
[Ref.: #68217]	polymyxin b ChEBI	yes	300	Unit (disc)
[Ref.: #68217]	rifampicin ChEBI	yes	30	µg (disc)
[Ref.: #68217]	streptomycin ChEBI	yes	10	µg (disc)
[Ref.: #68217]	tetracycline ChEBI	yes	30	µg (disc)
[Ref.: #68217]	vancomycin ChEBI	yes	30	µg (disc)
	Only first 10 entries are displayed. Click here to see all.

	Group ID of combined antibiotics	Metabolite	Antibiotic sensitivity	Concentration		Antibiotic resistance	Concentration
[Ref.: #68217]	10	amoxicillin ChEBI	yes	30	µg (disc)
[Ref.: #68217]	10	clavulanic acid ChEBI	yes	30	µg (disc)
[Ref.: #68217]	16	piperacillin ChEBI	yes	110	µg (disc)
[Ref.: #68217]	26	sulfamethoxazole ChEBI				yes	25	µg (disc)
[Ref.: #68217]	16	tazobactam ChEBI	yes	110	µg (disc)
[Ref.: #68217]	26	trimethoprim ChEBI				yes	25	µg (disc)

	Metabolite production	Metabolite	Production
[Ref.: #68217]		acetoin ChEBI	no
[Ref.: #68217]		indole ChEBI	no

	Physiological tests	Metabolite	Voges-Proskauer-test	Methylred-test
[Ref.: #68217]		acetoin ChEBI	-
[Ref.: #68217]		glucose ChEBI		-

	Enzyme	Enzyme activity	EC number
[Ref.: #68217]	alkaline phosphatase	+	3.1.3.1
[Ref.: #68217]	alpha-chymotrypsin	+	3.4.21.1
[Ref.: #68217]	arginine dihydrolase	-	3.5.3.6
[Ref.: #68217]	catalase	+	1.11.1.6
[Ref.: #68217]	cytochrome oxidase	+	1.9.3.1
[Ref.: #68217]	esterase (C 4)	+
[Ref.: #68217]	esterase Lipase (C 8)	+
[Ref.: #68217]	leucine arylamidase	+	3.4.11.1
[Ref.: #68217]	naphthol-AS-BI-phosphohydrolase	+
[Ref.: #68217]	urease	-	3.5.1.5
[Ref.: #68217]	valine arylamidase	+
	Only first 10 entries are displayed. Click here to see all.

Fatty acid profile

Metadata FA analysis

Reference

[Ref.: #68217]

Fatty acid	Percentage
C15:0 anteiso	51.4
C17:0 anteiso	17.8
C16:0 iso	11
C16:0	5.1
C14:0 anteiso	4
C15:0 iso	3.3
C14:0	1.3
C17:1 iso w5c	1.1
C17:0 iso	0.7
C16:1 w7c alcohol	0.7
C16:1 w11c	0.6
C18:0 iso	0.5
C17:0 iso 3OH	0.4
C15:0 2OH	0.4
C19:0 anteiso	0.32
C12:0	0.3
C13:0 anteiso	0.2
C16:1 w6c/C16:1 w7c	0.2
C18:3 w6,9,12c	0.1

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #67770]	Sample type/isolated from	Ka-pi, a traditional salted shrimp paste of Thailand

[Ref.: #68217]	Sample type/isolated from	Ka-pi, a traditional salted shrimp paste of Thailand
[Ref.: #68217]	Geographic location (country and/or sea, region)	Samut Sakhon Province
[Ref.: #68217]	Country	Thailand
[Ref.: #68217]	Country ISO 3 Code	THA
[Ref.: #68217]	Continent	Asia
[Ref.: #68217]	Geographic location	13.5452°/100.2760°
[Ref.: #68217]	Enrichment culture	JCM No. 377
[Ref.: #68217]	Enrichment culture duration	3-5 days
[Ref.: #68217]	Enrichment culture temperature	37 ̊C
* marker position based on {}

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #67770]	Lentibacillus sp. SSKP1-9 gene for 16S ribosomal RNA, partial sequence	LC367329	1445	ENA	2510307 tax ID

Genome sequence information:

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #67770]	Lentibacillus lipolyticus SSKP1-9	GCA_004145795	scaffold	GenBank	2510307 tax ID
[Ref.: #66792]	Lentibacillus sp. SSKP1-9	2510307.3	wgs	PATRIC	2510307 tax ID	*

[Ref.: #68217]	GC-content	44.6 mol%	genome sequence analysis
[Ref.: #67770]	GC-content	44.61 mol%	genome sequence analysis

Data predicted using genome information as a basis Genome-based predictions

	Trait	Prediction	Confidence in %	In training data
	Predictions from GenomeNet Sporulation v. 1 based on: Lentibacillus sp. SSKP1-9 PATRIC: 2510307.3
[Ref.: #69481]	spore-forming	yes	100	no

	Trait	Prediction	Confidence in %	In training data
	Predictions from Diaspora project based on: Lentibacillus sp. SSKP1-9 PATRIC: 2510307.3
[Ref.: #69480]	motile	yes	81.39	yes
[Ref.: #69480]	gram-positive	yes	85.791	no
[Ref.: #69480]	anaerobic	no	98.952	no
[Ref.: #69480]	aerobic	yes	90.81	yes
[Ref.: #69480]	halophile	yes	93.034	no
[Ref.: #69480]	spore-forming	yes	92.32	yes
[Ref.: #69480]	glucose-util	yes	74.803	yes
[Ref.: #69480]	thermophile	no	96.274	yes
[Ref.: #69480]	flagellated	yes	68.89	yes
[Ref.: #69480]	glucose-ferment	no	89.627	yes

Availability in culture collections External links

[Ref.: #67770]

Culture collection no.

JCM 32625, TISTR 2597

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Lentibacillus lipolyticus sp. nov., a moderately halophilic bacterium isolated from shrimp paste (Ka-pi).	Booncharoen A, Visessanguan W, Kuncharoen N, Yiamsombut S, Santiyanont P, Mhuantong W, Charoensri S, Rojsitthisak P, Tanasupawat S	Int J Syst Evol Microbiol	10.1099/ijsem.0.003658	2019	*
Phylogeny	Lentibacillus cibarius sp. nov., isolated from kimchi, a Korean fermented food.	Oh YJ, Kim JY, Jo HE, Park HK, Lim SK, Kwon MS, Choi HJ	J Microbiol	10.1007/s12275-020-9507-7	2020	*

References

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#67770 Japan Collection of Microorganism (JCM) ; Curators of the JCM;

#68217

Auttaporn Booncharoen, Wonnop Visessanguan, Nattakorn Kuncharoen, Supalurk Yiamsombut, Pannita Santiyanont, Wuttichai Mhuantong, Salisa Charoensri, Pornchai Rojsitthisak and Somboon Tanasupawat: Lentibacillus lipolyticus sp. nov., a moderately halophilic bacterium isolated from shrimp paste (Ka-pi). IJSEM 69: 3529 - 3536 2019 ( DOI 10.1099/ijsem.0.003658 )

#69480

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#69481

Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch: Predictions based on the model GenomeNet Sporulation v. 1 . ( DOI 10.21203/rs.3.rs-2527258/v1 )

* These data were automatically processed and therefore are not curated

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Lentibacillus sp. SSKP1-9

Lentibacillus sp. SSKP1-9

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