Name: Cryobacterium soli GCJ02
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 164251

Type strain:

Species: Cryobacterium soli

Strain Designation: GCJ02

Culture col. no.: JCM 32391, CCTCC M 2016604, MCCC 1K03549

Strain history: C. Gong; Hubei Univ. of Technol., China; GCJ02.

NCBI tax ID(s): 2220095 (species)

Section

Cryobacterium soli GCJ02 is an aerobe, psychrophilic, Gram-positive bacterium that has a yellow pigmentation and was isolated from Primordial forest soil.

Gram-positive
pigmented
rod-shaped
aerobe
psychrophilic
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Actinomycetota*
	Class	*Actinomycetes*
	Order	*Micrococcales*
	Family	*Microbacteriaceae*
	Genus	*Cryobacterium*
	Species	**Cryobacterium soli**
	Full Scientific Name (LPSN)	Cryobacterium soli Gong et al. 2020

Information on morphological and physiological properties Morphology

[Ref.: #69560]	Gram stain	positive

[Ref.: #69560]	Cell length	1.3-2.0 µm

[Ref.: #69560]	Cell width	0.3-0.5 µm

[Ref.: #69560]	Cell shape	rod-shaped

[Ref.: #69560]	Motility	no

[Ref.: #69560]	Colony size	2-3 mm
[Ref.: #69560]	Colony color	smooth yellow
[Ref.: #69560]	Incubation period	2 days
[Ref.: #69560]	Cultivation medium used	LB (Luria-Bertani) MEDIUM (DSMZ Medium 381)

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Pigmentation color

yellow

Information on culture and growth conditions Culture and growth conditions

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Culture medium

LB (Luria-Bertani) MEDIUM

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Culture medium growth

		Temperature
[Ref.: #67770]	growth	15 °C
[Ref.: #69560]	growth	4-26 °C
[Ref.: #69560]	optimum	18 °C

[Ref.: #67770]	Temperature range	psychrophilic
[Ref.: #69560]	Temperature range	psychrophilic

	pH	Kind of pH		pH
[Ref.: #69560]			optimum	7
[Ref.: #69560]			growth	5.0-11.0

Information on physiology and metabolism Physiology and metabolism

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Oxygen tolerance

aerobe

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Ability of spore formation

[Ref.: #67770]	Observation	quinones: MK-10
[Ref.: #69560]	Observation	The polar lipids are phosphatidylglycerol, diphosphatidylglycerol, some unidentified phospholipids and some unidentified polar lipids. The colonies with regular edges, slightly raised in the centre

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #69560]	adipate ChEBI	+	assimilation
[Ref.: #69560]	amygdalin ChEBI	-	fermentation
[Ref.: #69560]	arabinose ChEBI	+	fermentation
[Ref.: #69560]	cellulose ChEBI	-	hydrolysis
[Ref.: #69560]	citrate ChEBI	-	assimilation
[Ref.: #69560]	D-glucose ChEBI	-	fermentation
[Ref.: #69560]	D-glucose ChEBI	+	assimilation
[Ref.: #69560]	D-mannitol ChEBI	+	assimilation
[Ref.: #69560]	D-mannose ChEBI	+	assimilation
[Ref.: #69560]	esculin hydrolysate	+	reduction
[Ref.: #69560]	gelatin ChEBI	-	hydrolysis
[Ref.: #69560]	glucose ChEBI	+	fermentation
[Ref.: #69560]	hexanoate ChEBI	-	assimilation
[Ref.: #69560]	hydrogen sulfide ChEBI	-	assimilation
[Ref.: #69560]	L-arabinose ChEBI	+	assimilation
[Ref.: #69560]	malate ChEBI	+	assimilation
[Ref.: #69560]	maltose ChEBI	+	assimilation
[Ref.: #69560]	mannitol ChEBI	+	fermentation
[Ref.: #69560]	melibiose ChEBI	-	assimilation
[Ref.: #69560]	myo-inositol ChEBI	-	assimilation
[Ref.: #69560]	N-acetyl-D-glucosamine ChEBI	+	assimilation
[Ref.: #69560]	nitrate ChEBI	+	reduction
[Ref.: #69560]	phenylacetate ChEBI	-	assimilation
[Ref.: #69560]	potassium gluconate ChEBI	+	assimilation
[Ref.: #69560]	rhamnose ChEBI	-	assimilation
[Ref.: #69560]	skimmed milk	-	hydrolysis
[Ref.: #69560]	sodium citrate ChEBI	+	assimilation
[Ref.: #69560]	sorbitol ChEBI	-	assimilation
[Ref.: #69560]	starch ChEBI	-	hydrolysis
[Ref.: #69560]	sucrose ChEBI	+	fermentation
[Ref.: #69560]	tween 20 ChEBI	-	hydrolysis
[Ref.: #69560]	tween 40 ChEBI	-	hydrolysis
[Ref.: #69560]	tween 60 ChEBI	-	hydrolysis
[Ref.: #69560]	tween 80 ChEBI	-	hydrolysis
	Only first 10 entries are displayed. Click here to see all.

	Metabolite	Production
[Ref.: #69560]	acetoin ChEBI	no
[Ref.: #69560]	hydrogen sulfide ChEBI	no
[Ref.: #69560]	indole ChEBI	yes

	Physiological tests	Metabolite	Voges-Proskauer-test	Indole test
[Ref.: #69560]		acetoin ChEBI	-
[Ref.: #69560]		indole ChEBI		+

	Enzyme	Enzyme activity	EC number
[Ref.: #69560]	acid phosphatase	+	3.1.3.2
[Ref.: #69560]	alkaline phosphatase	-	3.1.3.1
[Ref.: #69560]	alpha-chymotrypsin	-	3.4.21.1
[Ref.: #69560]	alpha-fucosidase	-	3.2.1.51
[Ref.: #69560]	alpha-galactosidase	+	3.2.1.22
[Ref.: #69560]	alpha-glucosidase	+	3.2.1.20
[Ref.: #69560]	alpha-mannosidase	+	3.2.1.24
[Ref.: #69560]	arginine dihydrolase	-	3.5.3.6
[Ref.: #69560]	arginine dihydrolase	+	3.5.3.6
[Ref.: #69560]	beta-galactosidase	-	3.2.1.23
[Ref.: #69560]	beta-galactosidase	+	3.2.1.23
[Ref.: #69560]	beta-glucosidase	+	3.2.1.21
[Ref.: #69560]	beta-glucuronidase	-	3.2.1.31
[Ref.: #69560]	beta-N-acetylgalactosaminidase	-
[Ref.: #69560]	catalase	+	1.11.1.6
[Ref.: #69560]	cystine arylamidase	+	3.4.11.3
[Ref.: #69560]	cytochrome oxidase	+	1.9.3.1
[Ref.: #69560]	esterase (C 4)	+
[Ref.: #69560]	esterase Lipase (C 8)	-
[Ref.: #69560]	gelatinase	-
[Ref.: #69560]	leucine arylamidase	+	3.4.11.1
[Ref.: #69560]	lipase (C 14)	+
[Ref.: #69560]	lysine decarboxylase	-	4.1.1.18
[Ref.: #69560]	naphthol-AS-BI-phosphohydrolase	+
[Ref.: #69560]	ornithine decarboxylase	-	4.1.1.17
[Ref.: #69560]	trypsin	-	3.4.21.4
[Ref.: #69560]	tryptophan deaminase	+	4.1.99.1
[Ref.: #69560]	tryptophan deaminase	-	4.1.99.1
[Ref.: #69560]	urease	-	3.5.1.5
[Ref.: #69560]	valine arylamidase	+
	Only first 10 entries are displayed. Click here to see all.

Fatty acid profile

Metadata FA analysis

Reference

[Ref.: #69560]

Fatty acid	Percentage
C15:0 anteiso	53
C17:0 anteiso	18.8

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #67770]	Sample type/isolated from	Primordial forest (42°47' N, 127°08' E) soil
[Ref.: #67770]	Geographic location (country and/or sea, region)	Baishan City
[Ref.: #67770]	Country	China
[Ref.: #67770]	Country ISO 3 Code	CHN
[Ref.: #67770]	Continent	Asia
[Ref.: #67770]	Geographic location	42.7833°/127.1330°

[Ref.: #69560]	Sample type/isolated from	primordial forest (42° 47' N 127° 08' E), soil of Baishan city
[Ref.: #69560]	Geographic location (country and/or sea, region)	Baishan City
[Ref.: #69560]	Country	China
[Ref.: #69560]	Country ISO 3 Code	CHN
[Ref.: #69560]	Continent	Asia
* marker position based on {}

[Ref.: #69479]

Global distribution of 16S sequence MH376310 (>99% sequence identity) for Cryobacterium from Microbeatlas

Aquatic Samples	1251
Soil Samples	2872
Animal Samples	1381
Plant Samples	636
Total Samples	6140

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #67770]	Cryobacterium soli 16S ribosomal RNA gene, partial sequence	MH376310	1485	ENA	2220095 tax ID

Genome sequence information:

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #67770]	Cryobacterium soli GCJ02	GCA_003611035	complete	GenBank	2220095 tax ID
[Ref.: #69560]	genome sequence	CP030033.1		GenBank	2220095 tax ID

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GC-content

68.4 mol%

genome sequence analysis

Availability in culture collections External links

[Ref.: #67770]

Culture collection no.

JCM 32391, CCTCC M 2016604, MCCC 1K03549

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Cryobacterium soli sp. nov., isolated from forest soil.	Gong C, Lai Q, Cai H, Jiang Y, Liao H, Liu Y, Xue D	Int J Syst Evol Microbiol	10.1099/ijsem.0.003820	2020	*

References

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#67770 Japan Collection of Microorganism (JCM) ; Curators of the JCM;

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

#69560

Chunjie Gong, Qiliang Lai, Hanfeng Cai, Yanming Jiang, Hu Liao, Yunfei Liu and Dongsheng Xue: Cryobacterium soli sp. nov., isolated from forest soil. IJSEM 70: 2020 ( DOI 10.1099/ijsem.0.003820 )

* These data were automatically processed and therefore are not curated

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