Streptomyces thermocarboxydovorans (DSM 44296, IFO 16324, NBRC 16324)

Name: Streptomyces thermocarboxydovorans (DSM 44296, IFO 16324, NBRC 16324)
Creator: BacDive
License: http://creativecommons.org/licenses/by/4.0/

BacDive ID 16343

Type strain

Strain Designation AT52, AT 52

Culture col. no. DSM 44296 IFO 16324 NBRC 16324 AT52 NRRL B-24317 5 more

NCBI tax ID(s) 59298

Special Collections DSMZ JCM KCTC CIP Wink compendium

Links

Streptomyces thermocarboxydovorans AT52 is a spore-forming, thermophilic, Gram-positive prokaryote that builds an aerial mycelium and was isolated from allotment garden soil.

spore-forming Gram-positive rod-shaped thermophilic genome sequence 16S sequence

Name and taxonomic classification

SI-ID 50555

CIP 105544,DSM 44296,IFO 16324,JCM 10367,NCIMB 13530,KCTC 9938,NBRC 16324,LMG 19860,NRRL B-24317

@ref 20215

Last LPSN update 2025-12-16 09:50:50

Domain Bacillati

Phylum Actinomycetota

Class Actinomycetes

Order Kitasatosporales

Family Streptomycetaceae

Genus Streptomyces

Species Streptomyces thermocarboxydovorans

Full scientific name Streptomyces thermocarboxydovorans Kim et al. 1998

BacDive ID	Other strains from **Streptomyces thermocarboxydovorans** (2)	Type strain
16341	S. thermocarboxydovorans AT50, DSM 44294
16342	S. thermocarboxydovorans AT51, DSM 44295

Morphology

Cell morphology

@ref	Gram stain	Cell shape	Motility
123503	positive	rod-shaped

Colony morphology

@ref	Colony color	Incubation period	Medium used
20043	Dahlia yellow (1033)	10-14 days	ISP 2
20043	Colorless	10-14 days	ISP 3
20043	Colorless	10-14 days	ISP 4
20043	Colorless	10-14 days	ISP 5
20043	Colorless	10-14 days	ISP 6
20043	Colorless	10-14 days	ISP 7

Multicellular morphology

@ref	Complex name	Complex color	Medium name
20043	Aerial Mycelium	White	ISP 2
20043	Aerial Mycelium	White	ISP 3
20043	Aerial Mycelium	White	ISP 4
20043	Aerial Mycelium	White	ISP 5
20043			ISP 6
20043	Aerial Mycelium	White	ISP 7

Multimedia

@ref:	11653
multimedia content:	DSM_44296.jpg
multimedia.multimedia content:	https://www.dsmz.de/microorganisms/photos/DSM_44296.jpg
caption:	Medium 248 45°C
intellectual property rights:	© Leibniz-Institut DSMZ
manual_annotation:	1

Culture and growth conditions

Culture medium

@ref	Name	Medium link	Composition
11653	STARCH - MINERAL SALT - AGAR (STMS) (DSMZ Medium 252)	Medium recipe at MediaDive	Name: STARCH - MINERAL SALT - AGAR (STMS) (DSMZ Medium 252) Composition: Agar 14.985 g/l Starch 9.99001 g/l (NH4)2SO4 1.998 g/l CaCO3 1.998 g/l K2HPO4 0.999001 g/l MgSO4 x 7 H2O 0.999001 g/l NaCl 0.999001 g/l FeSO4 x 7 H2O 0.000999001 g/l MnCl2 x 4 H2O 0.000999001 g/l ZnSO4 x 7 H2O 0.000999001 g/l Distilled water
20043	ISP 2		Name: ISP 2 / Yeast Malt Agar (5265); 5265 Composition Malt extract 10.0 g/l Yeast extract 4.0 g/l Glucose 4.0 g/l Agar 15.0 g/l Preparation: Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.0 Usage: Maintenance and Taxonomy Organisms: All Actinomycetes
20043	ISP 3		Name: ISP 3; 5315 Composition Dog oat flakes 20.0 g/l Trace element solution (5314) 2.5 ml/l Agar 18.0 g/l Preparation: Oat flakes are cooked for 20 minutes, trace element solution and agar are added (in the case of non rolled oat flakes the suspension has to bee filtrated). Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.8 Usage: Maintenance and taxonomy (e.g. SEM As liquid medium for metabolite production) Organisms: All Actinomycetes Trace element solution 5314 Name: Trace element solution 5314; 5314 Composition CaCl2 x H2O 3.0 g/l Fe-III-citrate 1.0 g/l MnSO4 0.2 g/l ZnCl2 0.1 g/l CuSO4 x 5 H2O 0.025 g/l Sodium tetra borate 0.2 g/l CoCl2 x 6 H2O 0.004 g/l Sodium molybdate 0.01 g/l Preparation: Use double destillated water. Sterilisation: 20 minutes at 121°C pH before sterilisation: Usage: Trace element solution for different media Organisms:
20043	ISP 4		Name: ISP 4; DSM 547 Solution I: Difco soluble starch, 10.0 g. Make a paste of the starch with a small amount of cold distilled water and bring to a volume of 500 ml. Solution II: CaCO3 2.0 g K2HPO4 (anhydrous) 1.0 g MgSO4 x 7 H2O 1.0 g NaCl 1.0 g (NH4)2SO4 2.0 g Distilled water 500.0 ml Trace salt solution (see below) 1.0 ml The pH should be between 7.0 and 7.4. Do not adjust if it is within this range. Mix solutions I and II together. Add 20.0 g agar. Liquify agar by steaming at 100°C for 10 to 20 min. Trace element solution: FeSO4 x 7 H2O 0.1 g MnCl2 x 4 H2O 0.1 g ZnSO4 x 7 H2O 0.1 g Distilled water 100.0 ml
20043	ISP 5		Name: ISP 5 (5323) Composition L-Asparagine 1.0 g/l Glycerol 10.0 g/l K2HPO4 1.0 g/l Salt solution (see preparation) 1.0 ml/l Agar 20.0 g/l Preparation: Salt solution 1.0 g FeSO4 x 7 H2O 1.0 g MnCl2 x 4 H2O 1.0 g ZNSO4 x 7 H2O in 100 ml water Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.2 Usage: Maintenance and taxonomy Organisms: All Actinomycetes
20043	ISP 6		Name: ISP 6 (5318) Composition Peptone 15.0 g/l Proteose peptose 5.0 g/l Ferric ammonium citrate 0.5 g/l Sodium glycerophosphate 1.0 g/l Sodium thiosulfate 0.08 g/l Yeast extract 1.0 g/l Agar 15.0 g/l Sterilisation: 20 minutes at 121°C pH before sterilisation: Usage: Production of melanoid pigments Organisms: All Actinomycetes
20043	ISP 7		Name: ISP 7 (5322) Composition Glycerol 15.0 g/l L-Tyrosine 0.5 g/l L-Asparagine 1.0 g/l K2HPO4 0.5 g/l NaCl 0.5 g/l FeSO4 x 7 H2O 0.01 g/l Trace element solution 5343 1.0 ml/l Agar 20.0 Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.3 Usage: Production of melanoid pigments Organisms: All Actinomycetes
38562	MEDIUM 129 - for Pseudonocardia, Nocardia, Skermania and Streptomyces seoulensis, Streptomyces stramineus		Distilled water make up to (1000.000 ml);Agar (20.000 g);Glucose (10.000g);Yeast extract (1.000 g);Beef extract (1.000 g);Casamino acids (2.000 g)
11653	OAT FLAKES AGAR WITH YEAST EXTRACT (DSMZ Medium 248)	Medium recipe at MediaDive	Name: OAT FLAKES AGAR WITH YEAST EXTRACT (DSMZ Medium 248) Composition: Agar 20.0 g/l Oat flakes 20.0 g/l Yeast extract 2.0 g/l MnCl2 x 4 H2O 0.001 g/l FeSO4 x 7 H2O 0.001 g/l ZnSO4 x 7 H2O 0.001 g/l Distilled water
11653	BENNETT'S MODIFIED MEDIUM (P. AGRAWAL, UNPUBLISHED) (DSMZ Medium 894)	Medium recipe at MediaDive	Name: BENNETT'S MODIFIED MEDIUM (P. AGRAWAL, UNPUBLISHED) (DSMZ Medium 894) Composition: Agar 15.0 g/l Lab-Lemco beef extract 10.0 g/l Glucose 10.0 g/l Tryptose 2.0 g/l Yeast extract 2.0 g/l CaCO3 0.1 g/l Starch 0.1 g/l CoCl2 Ferric ammonium citrate Distilled water
123503	CIP Medium 129	Medium recipe at CIP

Culture temp

@ref	Growth	Type	Temperature (°C)	Range
11653	positive	growth	45	thermophilic
20043	positive	optimum	45	thermophilic
38562	positive	growth	45	thermophilic
67770	positive	growth	45	thermophilic
123503	positive	growth	25-45
123503	negative	growth	10

Physiology and metabolism

Spore formation

@ref	Spore description	Spore formation
20043	Formation of spore chains (rectiflixibilis), spore surface smooth

Halophily

@ref	Salt	Growth	Tested relation	Concentration
20043	NaCl	positive	maximum	5 %

Observation

67770

Observationquinones: MK-9(H₈)

Metabolite utilization

@ref	Chebi-ID	Metabolite	Utilization activity
20043	22599 ChEBI	arabinose	-
20043	62968 ChEBI	cellulose	-
123503	4853 ChEBI	esculin	-
20043	28757 ChEBI	fructose	+
20043	17234 ChEBI	glucose	-
123503	606565 ChEBI	hippurate	-
20043	29864 ChEBI	mannitol	+
20043	17268 ChEBI	myo-inositol	-
123503	17632 ChEBI	nitrate	+
123503	16301 ChEBI	nitrite	-
20043	16634 ChEBI	raffinose	-
20043	26546 ChEBI	rhamnose	-
20043	17992 ChEBI	sucrose	-
20043	18222 ChEBI	xylose	-

Metabolite production

@ref	Chebi-ID	Metabolite	Production
123503	35581 ChEBI	indole

Metabolite tests

@ref	Chebi-ID	Metabolite	Voges-proskauer-test
123503	15688 ChEBI	acetoin	-
123503	17234 ChEBI	glucose

Enzymes

@ref	Value	Activity	Ec
68382	acid phosphatase	+	3.1.3.2	from API zym
123503	alcohol dehydrogenase	-	1.1.1.1
68382	alkaline phosphatase	+	3.1.3.1	from API zym
68382	alpha-chymotrypsin	+	3.4.21.1	from API zym
68382	alpha-fucosidase	-	3.2.1.51	from API zym
68382	alpha-galactosidase	-	3.2.1.22	from API zym
68382	alpha-glucosidase	-	3.2.1.20	from API zym
68382	alpha-mannosidase	-	3.2.1.24	from API zym
123503	amylase	+
68382	beta-galactosidase	+	3.2.1.23	from API zym
123503	beta-galactosidase	+	3.2.1.23
68382	beta-glucosidase	-	3.2.1.21	from API zym
68382	beta-glucuronidase	-	3.2.1.31	from API zym
123503	caseinase	+	3.4.21.50
123503	catalase	+	1.11.1.6
68382	cystine arylamidase	+	3.4.11.3	from API zym
123503	DNase	+
68382	esterase (C 4)	+		from API zym
68382	esterase lipase (C 8)	+		from API zym
123503	gamma-glutamyltransferase	+	2.3.2.2
123503	gelatinase	+/-
123503	lecithinase	-
68382	leucine arylamidase	+	3.4.11.1	from API zym
123503	lipase	-
68382	lipase (C 14)	+		from API zym
123503	lysine decarboxylase	-	4.1.1.18
68382	N-acetyl-beta-glucosaminidase	+	3.2.1.52	from API zym
68382	naphthol-AS-BI-phosphohydrolase	+		from API zym
123503	ornithine decarboxylase	-	4.1.1.17
123503	oxidase	-
123503	phenylalanine ammonia-lyase	-	4.3.1.24
123503	protease	-
68382	trypsin	-	3.4.21.4	from API zym
123503	tryptophan deaminase	-
123503	tween esterase	+
123503	urease	-	3.5.1.5
68382	valine arylamidase	+		from API zym

API zym

@ref	Control	Alkaline phosphatase	Esterase (C 4)	2-naphtyl caprylateEsterase Lipase (C 8)	Lipase (C 14)	L-leucyl-2-naphthylamideLeucine arylamidase	L-valyl-2-naphthylamideValine arylamidase	L-cystyl-2-naphthylamideCystine arylamidase	Trypsin	alpha- Chymotrypsin	Acid phosphatase	Naphthol-AS-BI-phosphateNaphthol-AS-BI-phosphohydrolase	alpha- Galactosidase	beta- Galactosidase	beta- Glucuronidase	alpha- Glucosidase	beta- Glucosidase	N-acetyl-beta- glucosaminidase	alpha- Mannosidase	alpha- Fucosidase
123503	-	+	+	+	+	+	+	+	-	+	+	+	-	+	-	-	-	+	-	-

Isolation, sampling and environmental information

Isolation source categories

Cat1	Cat2	Cat3
#Engineered	#Agriculture	#Garden
#Environmental	#Terrestrial	#Soil

Isolation

@ref	Sample type	Geographic location	Country	Country ISO 3 Code	Continent
11653	allotment garden soil	Newcastle upon Tyne	United Kingdom	GBR	Europe
67770	Allotment garden soil		United Kingdom	GBR	Europe
123503	Environment, Allotment soil		United Kingdom	GBR	Europe

Interaction and safety

Risk assessment

@ref	Biosafety level	Biosafety level comment
11653	1	Risk group (German classification) According to TRBA 466
20043	1	German classification According to TRBA 466
123503	1	Risk group (French classification) According to TRBA 466

Sequence information

Genome sequences

@ref	Description	Assembly level	INSDC accession	NCBI tax ID	Score
124043	ASM3952463v1 assembly for Streptomyces thermocarboxydovorans JCM 10367	scaffold	GCA_039524635	59298	39.58

Genome browser: GCA_039524635

16S sequences

@ref	Description	Accession	Length	Database	NCBI tax ID
11653	Streptomyces thermocarboxydovorans DSM 44296 16S ribosomal RNA gene, complete sequence	U94489	1502		59298
11653	Streptomyces thermocarboxydovorans gene for 16S rRNA, partial sequence, strain: NBRC 16324	AB249929	1484		59298

GC content

@ref	GC-content (mol%)
11653	74.7
67770	74.7

Genome-based predictions

Literature

References

#11653	Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 44296
#20043	Wink, J.: Compendium of Actinobacteria. HZI-Helmholtz-Centre for Infection Research, Braunschweig .
#20215	Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )
#38562	; Curators of the CIP;
#67770	Japan Collection of Microorganism (JCM) ; Curators of the JCM;
#68382	Automatically annotated from API zym .
#123503	Collection of Institut Pasteur ; Curators of the CIP; CIP 105544
#124043	Isabel Schober, Julia Koblitz: Data extracted from sequence databases, automatically matched based on designation and taxonomy .
#126262	A. Lissin, I. Schober, J. F. Witte, H. Lüken, A. Podstawka, J. Koblitz, B. Bunk, P. Dawyndt, P. Vandamme, P. de Vos, J. Overmann, L. C. Reimer: StrainInfo—the central database for linked microbial strain identifiers. ( DOI 10.1093/database/baaf059 )

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