Streptomyces rochei (DSM 41454, ATCC 43682, INMI 40-31)

Colony morphology

Multicellular morphology

Multimedia

• @ref:	10318
  multimedia content:	DSM_41454.jpg
  multimedia.multimedia content:	https://www.dsmz.de/microorganisms/photos/DSM_41454.jpg
  caption:	Medium 65 28°C
  intellectual property rights:	© Leibniz-Institut DSMZ
  manual_annotation:	1

Culture medium

@ref	Name	Growth	Medium link	Composition
10318	GYM STREPTOMYCES MEDIUM (DSMZ Medium 65)		Medium recipe at MediaDive	Name: GYM STREPTOMYCES MEDIUM (DSMZ Medium 65) Composition: Agar 18.0 g/l Malt extract 10.0 g/l Yeast extract 4.0 g/l Glucose 4.0 g/l CaCO3 2.0 g/l Distilled water
18689	ISP 2			Name: ISP 2 / Yeast Malt Agar (5265); 5265 Composition Malt extract 10.0 g/l Yeast extract 4.0 g/l Glucose 4.0 g/l Agar 15.0 g/l Preparation: Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.0 Usage: Maintenance and Taxonomy Organisms: All Actinomycetes
18689	ISP 3			Name: ISP 3; 5315 Composition Dog oat flakes 20.0 g/l Trace element solution (5314) 2.5 ml/l Agar 18.0 g/l Preparation: Oat flakes are cooked for 20 minutes, trace element solution and agar are added (in the case of non rolled oat flakes the suspension has to bee filtrated). Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.8 Usage: Maintenance and taxonomy (e.g. SEM As liquid medium for metabolite production) Organisms: All Actinomycetes Trace element solution 5314 Name: Trace element solution 5314; 5314 Composition CaCl2 x H2O 3.0 g/l Fe-III-citrate 1.0 g/l MnSO4 0.2 g/l ZnCl2 0.1 g/l CuSO4 x 5 H2O 0.025 g/l Sodium tetra borate 0.2 g/l CoCl2 x 6 H2O 0.004 g/l Sodium molybdate 0.01 g/l Preparation: Use double destillated water. Sterilisation: 20 minutes at 121°C pH before sterilisation: Usage: Trace element solution for different media Organisms:
18689	ISP 7			Name: ISP 7 (5322) Composition Glycerol 15.0 g/l L-Tyrosine 0.5 g/l L-Asparagine 1.0 g/l K2HPO4 0.5 g/l NaCl 0.5 g/l FeSO4 x 7 H2O 0.01 g/l Trace element solution 5343 1.0 ml/l Agar 20.0 Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.3 Usage: Production of melanoid pigments Organisms: All Actinomycetes
18689	ISP 6			Name: ISP 6 (5318) Composition Peptone 15.0 g/l Proteose peptose 5.0 g/l Ferric ammonium citrate 0.5 g/l Sodium glycerophosphate 1.0 g/l Sodium thiosulfate 0.08 g/l Yeast extract 1.0 g/l Agar 15.0 g/l Sterilisation: 20 minutes at 121°C pH before sterilisation: Usage: Production of melanoid pigments Organisms: All Actinomycetes
18689	ISP 5			Name: ISP 5 (5323) Composition L-Asparagine 1.0 g/l Glycerol 10.0 g/l K2HPO4 1.0 g/l Salt solution (see preparation) 1.0 ml/l Agar 20.0 g/l Preparation: Salt solution 1.0 g FeSO4 x 7 H2O 1.0 g MnCl2 x 4 H2O 1.0 g ZNSO4 x 7 H2O in 100 ml water Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.2 Usage: Maintenance and taxonomy Organisms: All Actinomycetes
18689	ISP 4			Name: ISP 4; DSM 547 Solution I: Difco soluble starch, 10.0 g. Make a paste of the starch with a small amount of cold distilled water and bring to a volume of 500 ml. Solution II: CaCO3 2.0 g K2HPO4 (anhydrous) 1.0 g MgSO4 x 7 H2O 1.0 g NaCl 1.0 g (NH4)2SO4 2.0 g Distilled water 500.0 ml Trace salt solution (see below) 1.0 ml The pH should be between 7.0 and 7.4. Do not adjust if it is within this range. Mix solutions I and II together. Add 20.0 g agar. Liquify agar by steaming at 100°C for 10 to 20 min. Trace element solution: FeSO4 x 7 H2O 0.1 g MnCl2 x 4 H2O 0.1 g ZnSO4 x 7 H2O 0.1 g Distilled water 100.0 ml
10318	STARCH - MINERAL SALT - AGAR (STMS) (DSMZ Medium 252)		Medium recipe at MediaDive	Name: STARCH - MINERAL SALT - AGAR (STMS) (DSMZ Medium 252) Composition: Agar 20.0 g/l Starch 10.0 g/l (NH4)2SO4 2.0 g/l CaCO3 2.0 g/l K2HPO4 1.0 g/l MgSO4 x 7 H2O 1.0 g/l NaCl 1.0 g/l FeSO4 x 7 H2O 0.001 g/l MnCl2 x 4 H2O 0.001 g/l ZnSO4 x 7 H2O 0.001 g/l Distilled water

Culture temp

Spore formation

Halophily

Metabolite utilization

@ref	Chebi-ID	Metabolite	Utilization activity	Kind of utilization tested
68368	29016 ChEBI	arginine	+	hydrolysis	from API 20E
68368	16947 ChEBI	citrate	+	assimilation	from API 20E
68368	5291 ChEBI	gelatin	+	hydrolysis	from API 20E
68368	25094 ChEBI	lysine	+	degradation	from API 20E
68368	18257 ChEBI	ornithine	+	degradation	from API 20E
68368	27897 ChEBI	tryptophan	-	energy source	from API 20E
68368	16199 ChEBI	urea	+	hydrolysis	from API 20E

Metabolite production

@ref	Chebi-ID	Metabolite	Production
68368	15688 ChEBI	acetoin		from API 20E
68368	16136 ChEBI	hydrogen sulfide		from API 20E
68368	35581 ChEBI	indole		from API 20E

Metabolite tests

@ref	Chebi-ID	Metabolite	Voges-proskauer-test
68368	15688 ChEBI	acetoin	+	from API 20E
68368	35581 ChEBI	indole		from API 20E

Enzymes

@ref	Value	Activity	Ec
68368	arginine dihydrolase	+	3.5.3.6	from API 20E
68368	beta-galactosidase	+	3.2.1.23	from API 20E
68368	gelatinase	+		from API 20E
68368	lysine decarboxylase	+	4.1.1.18	from API 20E
68368	ornithine decarboxylase	+	4.1.1.17	from API 20E
68368	tryptophan deaminase	-	4.1.99.1	from API 20E
68368	urease	+	3.5.1.5	from API 20E

API 20E

Isolation

Taxonmaps

69479

Global distribution of 16S sequence AB920572 (>99% sequence identity) for Streptomyces from Microbeatlas

Aquatic Samples
Soil Samples	12
Animal Samples	16
Plant Samples
Total Samples	28

Risk assessment

Genome sequences

@ref	Description	Assembly level	INSDC accession	NCBI tax ID	Score
124043	ASM3953376v1 assembly for Streptomyces enissocaesilis JCM 9088	contig	GCA_039533765	332589	54.99

16S sequences

@ref	Description	Accession	Length	Database	NCBI tax ID
20218	Streptomyces enissocaesilis gene for 16S rRNA, partial sequence, strain: NBRC 100763	AB249930	1481		332589
20218	Streptomyces enissocaesilis strain NRRL B-16365 16S ribosomal RNA gene, partial sequence	DQ026641	1485		332589
124043	Streptomyces enissocaesilis strain NRRL B-16365(T) 16S ribosomal RNA gene, partial sequence.	MN687840	514		332589
124043	Streptomyces enissocaesilis strain NRRL B-16365(T) 16S ribosomal RNA gene, partial sequence.	MN686614	510		332589
124043	Streptomyces enissocaesilis strain NRRL B-16365(T) 16S ribosomal RNA gene, partial sequence.	MN686654	531		332589

Literature

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Reclassification of 15 Streptomyces species as synonyms of Streptomyces albogriseolus, Streptomyces althioticus, Streptomyces anthocyanicus, Streptomyces calvus, Streptomyces griseoincarnatus, Streptomyces mutabilis, Streptomyces pilosus or Streptomyces rochei.	Komaki H.	Int J Syst Evol Microbiol	10.1099/ijsem.0.004718	2019
	Taxonomy and Polyphasic Characterization of Alkaline Amylase Producing Marine Actinomycete Streptomyces rochei BTSS 1001.	Acharyabhatta A, Kandula SK, Terli R.	Int J Microbiol	10.1155/2013/276921	2013
Pathogenicity	Screening of endophytic Streptomycetes isolated from Parthenium hysterophorus L. against nosocomial pathogens.	Tanvir R, Sajid I, Hasnain S.	Pak J Pharm Sci		2013
	Specific Streptomyces strain enhances the growth, defensive mechanism, and fruit quality of cucumber by minimizing its fertilizer consumption.	Orouji E, Fathi Ghare Baba M, Sadeghi A, Gharanjik S, Koobaz P.	BMC Plant Biol	10.1186/s12870-023-04259-y	2023
	Diversity, mechanisms and beneficial features of phosphate-solubilizing Streptomyces in sustainable agriculture: A review.	Chouyia FE, Ventorino V, Pepe O.	Front Plant Sci	10.3389/fpls.2022.1035358	2022
	Multiple Potential Plant Growth Promotion Activities of Endemic Streptomyces spp. from Moroccan Sugar Beet Fields with Their Inhibitory Activities against Fusarium spp.	Aallam Y, Maliki BE, Dhiba D, Lemriss S, Souiri A, Haddioui A, Tarkka M, Hamdali H.	Microorganisms	10.3390/microorganisms9071429	2021
Metabolism	Biosynthesis of Ag, Se, and ZnO nanoparticles with antimicrobial activities against resistant pathogens using waste isolate Streptomyces enissocaesilis.	Shaaban M, El-Mahdy AM.	IET Nanobiotechnol	10.1049/iet-nbt.2017.0213	2018
Metabolism	A Bioactive Fraction from Streptomyces sp. Enhances Maize Tolerance against Drought Stress.	Warrad M, Hassan YM, Mohamed MSM, Hagagy N, Al-Maghrabi OA, Selim S, Saleh AM, AbdElgawad H.	J Microbiol Biotechnol	10.4014/jmb.2003.03034	2020
Phylogeny	Multiple strain analysis of Streptomyces species from Philippine marine sediments reveals intraspecies heterogeneity in antibiotic activities.	Tenebro CP, Trono DJVL, Vicera CVB, Sabido EM, Ysulat JA, Macaspac AJM, Tampus KA, Fabrigar TAP, Saludes JP, Dalisay DS.	Sci Rep	10.1038/s41598-021-96886-4	2021
	Microbial diversity in soils suppressive to Fusarium diseases.	Todorovic I, Moenne-Loccoz Y, Raicevic V, Jovicic-Petrovic J, Muller D.	Front Plant Sci	10.3389/fpls.2023.1228749	2023
	Growth Promotion and Disease Suppression Ability of a Streptomyces sp. CB-75 from Banana Rhizosphere Soil.	Chen Y, Zhou D, Qi D, Gao Z, Xie J, Luo Y.	Front Microbiol	10.3389/fmicb.2017.02704	2017
Pathogenicity	Purification and characterization of salvianolic acid B from Streptomyces sp. M4 possessing antifungal activity against fungal phytopathogens.	Sharma M, Manhas RK.	Microbiol Res	10.1016/j.micres.2020.126478	2020
Enzymology	Endophytic actinomycetes from spontaneous plants of Algerian Sahara: indole-3-acetic acid production and tomato plants growth promoting activity.	Goudjal Y, Toumatia O, Sabaou N, Barakate M, Mathieu F, Zitouni A.	World J Microbiol Biotechnol	10.1007/s11274-013-1344-y	2013
	Halotolerant Marine Rhizosphere-Competent Actinobacteria Promote Salicornia bigelovii Growth and Seed Production Using Seawater Irrigation.	Mathew BT, Torky Y, Amin A, Mourad AI, Ayyash MM, El-Keblawy A, Hilal-Alnaqbi A, AbuQamar SF, El-Tarabily KA.	Front Microbiol	10.3389/fmicb.2020.00552	2020