Name: Streptomyces megasporus DSM 41476
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 16186

Type strain:

Species: Streptomyces megasporus

Culture col. no.: DSM 41476, ATCC 43688, HUT 6610, IFO 14749, INA M-22, INMI 2869, JCM 6926, NBRC 14749, NRRL B-16372, VKM Ac-1776

Strain history: INMI 2869.

NCBI tax ID(s): 44060 (species)

SI-ID 92985

ATCC 43688, DSM 41450, DSM 41476, JCM 6306, IFO 14749, NCIMB 12472, JCM 6926, HUT 6610, NRRL B-16372, VKM Ac-1776, NBRC 14749

Other strains from Streptomyces megasporus

S. megasporus A 1202, DSM 41450, INMI 1869, NCIMB ...

Section

Streptomyces megasporus DSM 41476 is a thermophilic bacterium that builds an aerial mycelium.

thermophilic
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Actinomycetota*
	Class	*Actinomycetes*
	Order	*Kitasatosporales*
	Family	*Streptomycetaceae*
	Genus	*Streptomyces*
	Species	**Streptomyces megasporus**
	Full Scientific Name (LPSN)	Streptomyces megasporus (ex Krassilnikov et al. 1968) Agre 1986

Information on morphological and physiological properties Morphology

[Ref.: #18696]	Colony color	Beige (1001)
[Ref.: #18696]	Incubation period	10-14 days
[Ref.: #18696]	Cultivation medium used	ISP 2

[Ref.: #18696]	Colony color	Colorless
[Ref.: #18696]	Incubation period	10-14 days
[Ref.: #18696]	Cultivation medium used	ISP 3

[Ref.: #18696]	Colony color	Colorless
[Ref.: #18696]	Incubation period	10-14 days
[Ref.: #18696]	Cultivation medium used	ISP 4

[Ref.: #18696]	Colony color	Colorless
[Ref.: #18696]	Incubation period	10-14 days
[Ref.: #18696]	Cultivation medium used	ISP 5

[Ref.: #18696]	Colony color	Colorless
[Ref.: #18696]	Incubation period	10-14 days
[Ref.: #18696]	Cultivation medium used	ISP 6

[Ref.: #18696]	Colony color	Beige (1001)
[Ref.: #18696]	Incubation period	10-14 days
[Ref.: #18696]	Cultivation medium used	ISP 7

[Ref.: #18696]	Multicellular complex forming ability	yes
[Ref.: #18696]	Multicellular complex name	Aerial Mycelium
[Ref.: #18696]	Multicellular complex color	White
[Ref.: #18696]	Medium Name (multicellularity)	ISP 2

[Ref.: #18696]	Multicellular complex forming ability	no
[Ref.: #18696]	Medium Name (multicellularity)	ISP 3

[Ref.: #18696]	Multicellular complex forming ability	no
[Ref.: #18696]	Medium Name (multicellularity)	ISP 4

[Ref.: #18696]	Multicellular complex forming ability	no
[Ref.: #18696]	Medium Name (multicellularity)	ISP 5

[Ref.: #18696]	Multicellular complex forming ability	yes
[Ref.: #18696]	Multicellular complex name	Aerial Mycelium
[Ref.: #18696]	Multicellular complex color	Sparse, white
[Ref.: #18696]	Medium Name (multicellularity)	ISP 6

[Ref.: #18696]	Multicellular complex forming ability	no
[Ref.: #18696]	Medium Name (multicellularity)	ISP 7

Information on culture and growth conditions Culture and growth conditions

[Ref.: #10340]

Culture medium

V-8 JUICE AGAR (DSMZ Medium 310)

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Culture medium growth

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Culture medium link

Medium recipe at MediaDive

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Culture medium composition

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Culture medium

GYM STREPTOMYCES MEDIUM (DSMZ Medium 65)

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Culture medium growth

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Culture medium link

Medium recipe at MediaDive

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Culture medium composition

expand / minimize

[Ref.: #18696]

Culture medium

ISP 2

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Culture medium growth

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Culture medium composition

expand / minimize

[Ref.: #18696]

Culture medium

ISP 7

[Ref.: #18696]

Culture medium growth

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Culture medium composition

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[Ref.: #18696]

Culture medium

ISP 6

[Ref.: #18696]

Culture medium growth

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Culture medium composition

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[Ref.: #18696]

Culture medium

ISP 5

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Culture medium growth

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Culture medium composition

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[Ref.: #18696]

Culture medium

ISP 4

[Ref.: #18696]

Culture medium growth

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Culture medium composition

expand / minimize

[Ref.: #18696]

Culture medium

ISP 3

[Ref.: #18696]

Culture medium growth

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Culture medium composition

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		Temperature
[Ref.: #10340]	growth	45 °C
[Ref.: #18696]	optimum	28 °C
[Ref.: #67770]	growth	40 °C

[Ref.: #10340]	Temperature range	thermophilic
[Ref.: #18696]	Temperature range	mesophilic
[Ref.: #67770]	Temperature range	thermophilic

Information on physiology and metabolism Physiology and metabolism

	Halophily	Salt	Tested relation		Salt conc.
[Ref.: #18696]		NaCl		maximum	2.5	%

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #68368]	arginine ChEBI	+	hydrolysis	* from API 20E
[Ref.: #68368]	citrate ChEBI	+	assimilation	* from API 20E
[Ref.: #68368]	gelatin ChEBI	+	hydrolysis	* from API 20E
[Ref.: #68368]	lysine ChEBI	+	degradation	* from API 20E
[Ref.: #68368]	ornithine ChEBI	+	degradation	* from API 20E
[Ref.: #68368]	tryptophan ChEBI	-	energy source	* from API 20E
[Ref.: #68368]	urea ChEBI	+	hydrolysis	* from API 20E

	Metabolite	Production
[Ref.: #68368]	acetoin ChEBI	no	* from API 20E
[Ref.: #68368]	hydrogen sulfide ChEBI	no	* from API 20E
[Ref.: #68368]	indole ChEBI	no	* from API 20E

	Physiological tests	Metabolite	Voges-Proskauer-test	Indole test
[Ref.: #68368]		acetoin ChEBI	-		* from API 20E
[Ref.: #68368]		indole ChEBI		-	* from API 20E

	Enzyme	Enzyme activity	EC number
[Ref.: #68368]	arginine dihydrolase	+	3.5.3.6	* from API 20E
[Ref.: #68368]	beta-galactosidase	+	3.2.1.23	* from API 20E
[Ref.: #68368]	gelatinase	+		* from API 20E
[Ref.: #68368]	lysine decarboxylase	+	4.1.1.18	* from API 20E
[Ref.: #68368]	ornithine decarboxylase	+	4.1.1.17	* from API 20E
[Ref.: #68368]	tryptophan deaminase	-	4.1.99.1	* from API 20E
[Ref.: #68368]	urease	+	3.5.1.5	* from API 20E

API® 20E:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	beta-GalactosidaseONPG	Arginine dihydrolaseADH (Arg)	Lysine decarboxylaseLDC (Lys)	Ornithine decarboxylaseODC	Citrate utilizationCIT	H2S productionH2S	Urease/urea hydrolysisURE	Tryptophan deaminaseTDA (Trp)	Indole productionIND	Acetoin production (Voges Proskauer test)VP	Gelatinase/gelatin hydrolysisGEL	Fermentation/oxidation of D-glucoseGLU	Fermentation/oxidation of D-mannitolMAN	Fermentation/oxidation of inositolINO	Fermentation/oxidation of sorbitolSor	Fermentation/oxidation of L-rhamnoseRHA	Fermentation/oxidation of sucroseSAC	Fermentation/oxidation of melibioseMEL	Fermentation/oxidation of amygdalinAMY	Fermentation/oxidation of L-arabinoseARA	Cytochrome oxidaseOX	Nitrite productionNO2	Reduction to N2N2	MotilityMOB	Growth on MacConkey mediumMAC	Oxidative catabolisation of D-glucoseOF-O	Fermentation of D-glucoseOF-F
[Ref.: #18696]	204	+	+	+	+	+	-	+	-	-	-	+	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #69479]

Global distribution of 16S sequence Z68100 (>99% sequence identity) for Streptomyces megasporus subclade from Microbeatlas

Aquatic Samples	4
Soil Samples	13
Animal Samples	1
Plant Samples	1
Total Samples	19

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #18696]	Biosafety level	1	German classification
[Ref.: #10340]	Biosafety level	1	Risk group (German classification) According to TRBA 466

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #20218]	S.megasporus 16S rRNA gene	Z68100	1490	ENA	44060 tax ID	*
[Ref.: #20218]	Streptomyces megasporus gene for 16S rRNA, partial sequence, strain: NBRC 14749	AB184617	1468	ENA	44060 tax ID	*

Genome sequence information:

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #67770]	Streptomyces megasporus NRRL B-16372	GCA_000718985	contig	GenBank	44060 tax ID

[Ref.: #67770]

GC-content

72.3 mol%

genome sequence analysis

Data predicted using genome information as a basis Genome-based predictions

	Trait	Prediction	Confidence in %	In training data
	Predictions from Diaspora project based on: Streptomyces megasporus NRRL B-16372 NCBI: GCA_000718985.1
[Ref.: #69480]	motile	no	94.811	no
[Ref.: #69480]	flagellated	no	98.011	no
[Ref.: #69480]	gram-positive	yes	90.84	no
[Ref.: #69480]	anaerobic	no	98.337	no
[Ref.: #69480]	halophile	no	84.693	no
[Ref.: #69480]	spore-forming	yes	93.066	no
[Ref.: #69480]	thermophile	no	95.629	no
[Ref.: #69480]	glucose-util	yes	88.613	no
[Ref.: #69480]	aerobic	yes	91.337	no
[Ref.: #69480]	glucose-ferment	no	89.926	no

Availability in culture collections External links

[Ref.: #10340]

Culture collection no.

DSM 41476, ATCC 43688, HUT 6610, IFO 14749, INA M-22, INMI 2869, JCM 6926, NBRC 14749, NRRL B-16372, VKM Ac-1776

[Ref.: #85248]

SI-ID 92985

Literature:

Topic	Title	Authors	Journal	DOI	Year
Metabolism	A new xylanase from Streptomyces megasporus DSM 41476 with high yield of xylobiose.	Shi P, Qiu Z, Bai Y, Yuan T, Huang H, Pan X, Yang P, Zhang W, Yao B	World J Microbiol Biotechnol	10.1007/s11274-011-0863-7	2011	*
Phylogeny	Streptomyces barkulensis sp. nov., isolated from an estuarine lake.	Ray L, Mishra SR, Panda AN, Rastogi G, Pattanaik AK, Adhya TK, Suar M, Raina V	Int J Syst Evol Microbiol	10.1099/ijs.0.056614-0	2014	*
Phylogeny	Streptomyces radiopugnans sp. nov., a radiation-resistant actinomycete isolated from radiation-polluted soil in China.	Mao J, Tang Q, Zhang Z, Wang W, Wei D, Huang Y, Liu Z, Shi Y, Goodfellow M	Int J Syst Evol Microbiol	10.1099/ijs.0.65027-0	2007	*
	A xylanase with broad pH and temperature adaptability from Streptomyces megasporus DSM 41476, and its potential application in brewing industry.	Qiu Z, Shi P, Luo H, Bai Y, Yuan T, Yang P, Liu S, Yao B	Enzyme Microb Technol	10.1016/j.enzmictec.2010.02.003	2010	*

References

#10340

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 41476

#18696

Wink, J.: Compendium of Actinobacteria. HZI-Helmholtz-Centre for Infection Research, Braunschweig .

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#20218

Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.: StrainInfo introduces electronic passports for microorganisms.. Syst Appl Microbiol. 37: 42 - 50 2014 ( DOI 10.1016/j.syapm.2013.11.002 , PubMed 24321274 )

#67770 Japan Collection of Microorganism (JCM) ; Curators of the JCM;

#68368

Automatically annotated from API 20E .

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

#69480

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#85248

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID92985.1 )

* These data were automatically processed and therefore are not curated

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Streptomyces megasporus NRRL B-16372

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