Streptomyces ardus (DSM 40527, ATCC 27417, CBS 731.72)

Name: Streptomyces ardus (DSM 40527, ATCC 27417, CBS 731.72)
Creator: BacDive
License: http://creativecommons.org/licenses/by/4.0/

BacDive ID 16146

Type strain

Culture col. no. DSM 40527 ATCC 27417 CBS 731.72 IFO 13430 ISP 5527 17 more

NCBI tax ID(s) 67273

Special Collections DSMZ JCM KCTC CIP Wink compendium

History <- E.B. Shirling, ISP <- NRRL <- A. Dietz, Upjohn Co., UC 2500 KCC S-0543 <-- IPV 2020 <-- Upjohn Co., USA; UC 2500. CIP <- 2003, JCM <- 1983, KCC <- IFO <- SAJ <- ISP <- NRRL <- UC: strain UC 2500

Links

Streptomyces ardus DSM 40527 is an obligate aerobe, mesophilic, Gram-positive prokaryote that builds an aerial mycelium and produces antibiotic compounds.

antibiotic compound production Gram-positive rod-shaped obligate aerobe mesophilic 16S sequence

Name and taxonomic classification

SI-ID 38996

ATCC 27417,IFO 13430,NRRL2817,JCM 4543,JCM 4722,CCRC 12319,VKM Ac-930,NRRL B-2817,CECT 3254,KCTC 9887,AS 4.167,NBRC 13430,BCRC 12319,CIP 108147,NBIMCC 1592,CGMCC 4.1670,CBS 731.72,DSM 40527

@ref 20215

Last LPSN update 2026-02-09 11:21:54

Domain Bacteria

Phylum Actinomycetota

Class Actinomycetes

Order Kitasatosporales

Family Streptomycetaceae

Genus Streptomyces

Species Streptomyces ardus

Full scientific name Streptomyces ardus (De Boer et al. 1961) Witt and Stackebrandt 1991

Synonyms (2)

"Streptomyces ardus"
Streptoverticillium ardum

BacDive ID	Other strains from **Streptomyces ardus** (1)	Type strain
16147	S. ardus DSM 40603, ATCC 27422, CBS 791.72, IFO 13490, ...

Morphology

Cell morphology

@ref	Gram stain	Cell shape	Motility
116482	positive	rod-shaped

Colony morphology

@ref	Colony color	Incubation period	Medium used
19471	Beige	10-14 days	ISP 2
19471	Beige	10-14 days	ISP 3
19471	Beige	10-14 days	ISP 4
19471	Beige	10-14 days	ISP 5
19471	Beige	10-14 days	ISP 6
19471	Beige	10-14 days	ISP 7
116482

Multicellular morphology

@ref	Complex name	Medium name
19471		ISP 2
19471		ISP 3
19471		ISP 4
19471		ISP 5
19471	Aerial Mycelium	ISP 6
19471		ISP 7

Multimedia

@ref:	9616
multimedia content:	DSM_40527.jpg
multimedia.multimedia content:	https://www.dsmz.de/microorganisms/photos/DSM_40527.jpg
caption:	Medium 65 28°C
intellectual property rights:	© Leibniz-Institut DSMZ
manual_annotation:	1

Culture and growth conditions

Culture medium

@ref	Name	Composition	Medium link
19471	ISP 2	Name: ISP 2 / Yeast Malt Agar (5265); 5265 Composition Malt extract 10.0 g/l Yeast extract 4.0 g/l Glucose 4.0 g/l Agar 15.0 g/l Preparation: Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.0 Usage: Maintenance and Taxonomy Organisms: All Actinomycetes
19471	ISP 3	Name: ISP 3; 5315 Composition Dog oat flakes 20.0 g/l Trace element solution (5314) 2.5 ml/l Agar 18.0 g/l Preparation: Oat flakes are cooked for 20 minutes, trace element solution and agar are added (in the case of non rolled oat flakes the suspension has to bee filtrated). Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.8 Usage: Maintenance and taxonomy (e.g. SEM As liquid medium for metabolite production) Organisms: All Actinomycetes Trace element solution 5314 Name: Trace element solution 5314; 5314 Composition CaCl2 x H2O 3.0 g/l Fe-III-citrate 1.0 g/l MnSO4 0.2 g/l ZnCl2 0.1 g/l CuSO4 x 5 H2O 0.025 g/l Sodium tetra borate 0.2 g/l CoCl2 x 6 H2O 0.004 g/l Sodium molybdate 0.01 g/l Preparation: Use double destillated water. Sterilisation: 20 minutes at 121°C pH before sterilisation: Usage: Trace element solution for different media Organisms:
19471	ISP 4	Name: ISP 4; DSM 547 Solution I: Difco soluble starch, 10.0 g. Make a paste of the starch with a small amount of cold distilled water and bring to a volume of 500 ml. Solution II: CaCO3 2.0 g K2HPO4 (anhydrous) 1.0 g MgSO4 x 7 H2O 1.0 g NaCl 1.0 g (NH4)2SO4 2.0 g Distilled water 500.0 ml Trace salt solution (see below) 1.0 ml The pH should be between 7.0 and 7.4. Do not adjust if it is within this range. Mix solutions I and II together. Add 20.0 g agar. Liquify agar by steaming at 100°C for 10 to 20 min. Trace element solution: FeSO4 x 7 H2O 0.1 g MnCl2 x 4 H2O 0.1 g ZnSO4 x 7 H2O 0.1 g Distilled water 100.0 ml
19471	ISP 5	Name: ISP 5 (5323) Composition L-Asparagine 1.0 g/l Glycerol 10.0 g/l K2HPO4 1.0 g/l Salt solution (see preparation) 1.0 ml/l Agar 20.0 g/l Preparation: Salt solution 1.0 g FeSO4 x 7 H2O 1.0 g MnCl2 x 4 H2O 1.0 g ZNSO4 x 7 H2O in 100 ml water Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.2 Usage: Maintenance and taxonomy Organisms: All Actinomycetes
19471	ISP 6	Name: ISP 6 (5318) Composition Peptone 15.0 g/l Proteose peptose 5.0 g/l Ferric ammonium citrate 0.5 g/l Sodium glycerophosphate 1.0 g/l Sodium thiosulfate 0.08 g/l Yeast extract 1.0 g/l Agar 15.0 g/l Sterilisation: 20 minutes at 121°C pH before sterilisation: Usage: Production of melanoid pigments Organisms: All Actinomycetes
19471	ISP 7	Name: ISP 7 (5322) Composition Glycerol 15.0 g/l L-Tyrosine 0.5 g/l L-Asparagine 1.0 g/l K2HPO4 0.5 g/l NaCl 0.5 g/l FeSO4 x 7 H2O 0.01 g/l Trace element solution 5343 1.0 ml/l Agar 20.0 Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.3 Usage: Production of melanoid pigments Organisms: All Actinomycetes
33898	MEDIUM 57 - for Streptomyces, Nocardioides, Lentzea albidocapillata and Streptoverticillium reticulum	Distilled water make up to (1000.000 ml);Agar (15.000 g);Glucose (4.000g);Yeast extract (4.000 g);Malt extract (10.000 g);Calcium carbonate (2.000 g)
116482	CIP Medium 57		Medium recipe at CIP

Culture temp

@ref	Growth	Type	Temperature (°C)	Range
19471	positive	optimum	28	mesophilic
33898	positive	growth	30	mesophilic
67770	positive	growth	28	mesophilic
116482	positive	growth	25-37	mesophilic
116482	negative	growth	10
116482	negative	growth	41
116482	negative	growth	45

Physiology and metabolism

Oxygen tolerance

116482

Oxygen toleranceobligate aerobe

Compound production

@ref	Compound
9616	porfiromycin
19471	Porfiromycin (Methylmitomycin C)

Halophily

@ref	Salt	Growth	Tested relation	Concentration
116482	NaCl	positive	growth	0-4 %
116482	NaCl		growth	6 %
116482	NaCl		growth	8 %
116482	NaCl		growth	10 %

Metabolite utilization

@ref	Chebi-ID	Metabolite	Utilization activity	Kind of utilization tested
19471	22599 ChEBI	arabinose	-
68368	29016 ChEBI	arginine	+	hydrolysis	from API 20E
19471	62968 ChEBI	cellulose	-
116482	16947 ChEBI	citrate	-	carbon source
68368	16947 ChEBI	citrate	+	assimilation	from API 20E
116482	4853 ChEBI	esculin	-	hydrolysis
19471	28757 ChEBI	fructose	+
68368	5291 ChEBI	gelatin	+	hydrolysis	from API 20E
19471	17234 ChEBI	glucose	+
116482	606565 ChEBI	hippurate	+	hydrolysis
68368	25094 ChEBI	lysine	+	degradation	from API 20E
19471	29864 ChEBI	mannitol	-
19471	17268 ChEBI	myo-inositol	+
116482	17632 ChEBI	nitrate	-	reduction
116482	17632 ChEBI	nitrate	-	respiration
116482	16301 ChEBI	nitrite	-	reduction
68368	18257 ChEBI	ornithine	+	degradation	from API 20E
19471	16634 ChEBI	raffinose	-
19471	26546 ChEBI	rhamnose	-
19471	17992 ChEBI	sucrose	-
68368	27897 ChEBI	tryptophan	-	energy source	from API 20E
68368	16199 ChEBI	urea	+	hydrolysis	from API 20E
19471	18222 ChEBI	xylose	-

Metabolite production

@ref	Chebi-ID	Metabolite
68368	15688 ChEBI	acetoin	from API 20E
68368	16136 ChEBI	hydrogen sulfide	from API 20E
116482	35581 ChEBI	indole
68368	35581 ChEBI	indole	from API 20E
67770		porfiromycin

Metabolite tests

@ref	Chebi-ID	Metabolite	Voges-proskauer-test	Methylred-test	Indole test
116482	15688 ChEBI	acetoin	-
68368	15688 ChEBI	acetoin	-			from API 20E
116482	17234 ChEBI	glucose		-
68368	35581 ChEBI	indole			-	from API 20E

Enzymes

@ref	Value	Activity	Ec
68382	acid phosphatase	+	3.1.3.2	from API zym
116482	alcohol dehydrogenase	-	1.1.1.1
68382	alkaline phosphatase	+	3.1.3.1	from API zym
68382	alpha-chymotrypsin	+	3.4.21.1	from API zym
68382	alpha-fucosidase	-	3.2.1.51	from API zym
68382	alpha-galactosidase	-	3.2.1.22	from API zym
68382	alpha-glucosidase	+	3.2.1.20	from API zym
68382	alpha-mannosidase	+	3.2.1.24	from API zym
116482	amylase	-
68368	arginine dihydrolase	+	3.5.3.6	from API 20E
68382	beta-galactosidase	-	3.2.1.23	from API zym
116482	beta-galactosidase	-	3.2.1.23
68368	beta-galactosidase	+	3.2.1.23	from API 20E
68382	beta-glucuronidase	-	3.2.1.31	from API zym
116482	caseinase	-	3.4.21.50
116482	catalase	+	1.11.1.6
68382	cystine arylamidase	+	3.4.11.3	from API zym
116482	DNase	-
68382	esterase (C 4)	+		from API zym
68382	esterase lipase (C 8)	+		from API zym
116482	gamma-glutamyltransferase	+	2.3.2.2
116482	gelatinase	-
68368	gelatinase	+		from API 20E
116482	lecithinase	+
68382	leucine arylamidase	+	3.4.11.1	from API zym
116482	lipase	+
68382	lipase (C 14)	-		from API zym
116482	lysine decarboxylase	-	4.1.1.18
68368	lysine decarboxylase	+	4.1.1.18	from API 20E
68382	naphthol-AS-BI-phosphohydrolase	+		from API zym
116482	ornithine decarboxylase	-	4.1.1.17
68368	ornithine decarboxylase	+	4.1.1.17	from API 20E
116482	oxidase	-
116482	phenylalanine ammonia-lyase	-	4.3.1.24
68382	trypsin	+	3.4.21.4	from API zym
116482	tryptophan deaminase	-
68368	tryptophan deaminase	-	4.1.99.1	from API 20E
116482	tween esterase	-
116482	urease	+	3.5.1.5
68368	urease	+	3.5.1.5	from API 20E
68382	valine arylamidase	+		from API zym

API 20E

@ref	ONPG	ADH (Arg)	LDC (Lys)	ODC	CIT	H2S productionH2S	URE	TDA (Trp)	IND	Acetoin production (Voges Proskauer test)VP	GEL	GLU	MAN	INO	Sor	RHA	SAC	MEL	AMY	ARA	OX	Nitrite productionNO2	Reduction to N2N2	MotilityMOB	Growth on MacConkey mediumMAC	OF-O	OF-F
19471	+	+	+	+	+	+	+	-	-	-	+	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.

API zym

@ref	Control	Alkaline phosphatase	Esterase (C 4)	2-naphtyl caprylateEsterase Lipase (C 8)	Lipase (C 14)	L-leucyl-2-naphthylamideLeucine arylamidase	L-valyl-2-naphthylamideValine arylamidase	L-cystyl-2-naphthylamideCystine arylamidase	Trypsin	alpha- Chymotrypsin	Acid phosphatase	Naphthol-AS-BI-phosphateNaphthol-AS-BI-phosphohydrolase	alpha- Galactosidase	beta- Galactosidase	beta- Glucuronidase	alpha- Glucosidase	beta- Glucosidase	N-acetyl-beta- glucosaminidase	alpha- Mannosidase	alpha- Fucosidase
19471	not determinedn.d.	+	+	+	-	+	+	+	+	+	+	+	-	-	-	+	+	+	+	-
116482	-	+	+	+	-	+	+	+	+	+	+	+	-	-	-	+	-	-	+	-

API biotype100

@ref	ControlQ	D-glucose as carbon sourceGLU	D-fructose as carbon sourceFRU	D-galactose as carbon sourceGAL	D-trehalose as carbon sourceTRE	D-mannose as carbon sourceMNE	L-sorbose as carbon sourceSBE	D-melibiose as carbon sourceMEL	sucrose as carbon sourceSAC	D-raffinose as carbon sourceRAF	maltotriose as carbon sourceMTE	maltose as carbon sourceMAL	lactose as carbon sourceLAC	lactulose as carbon sourceLTE	1-O-methyl-beta-galactopyranoside as carbon sourceMbGa	1-O-methyl-alpha-galactopyranoside as carbon sourceMaGa	D-cellobiose as carbon sourceCEL	gentiobiose as carbon sourceGEN	1-O-methyl-beta-D-glucopyranoside as carbon sourceMbGu	esculin as carbon sourceESC	D-ribose as carbon sourceRIB	L-arabinose as carbon sourceARA	D-xylose as carbon sourceXYL	palatinose as carbon sourcePLE	L-rhamnose as carbon sourceRHA	L-fucose as carbon sourceFUC	D-melezitose as carbon sourceMLZ	D-arabitol as carbon sourceDARL	L-arabitol as carbon sourceLARL	xylitol as carbon sourceXLT	dulcitol as carbon sourceDUL	D-tagatose as carbon sourceTAG	glycerol as carbon sourceGLY	myo-inositol as carbon sourceINO	D-mannitol as carbon sourceMAN	maltitol as carbon sourceMTL	D-turanose as carbon sourceTUR	D-sorbitol as carbon sourceSOR	adonitol as carbon sourceADO	hydroxyquinoline-beta-glucuronide as carbon sourceHBG	D-lyxose as carbon sourceLYX	i-erythritol as carbon sourceERY	1-O-methyl-alpha-D-glucoside as carbon sourceMDG	3-O-methyl-D-glucose as carbon source3MDG	D-saccharate as carbon sourceSAT	mucate as carbon sourceMUC	L-tartrate as carbon sourceLTAT	D-tartrate as carbon sourceDTAT	meso-tartrate as carbon sourceMTAT	D-malate as carbon sourceDMLT	L-malate as carbon sourceLMLT	cis-aconitate as carbon sourceCATE	trans-aconitate as carbon sourceTATE	tricarballylate as carbon sourceTTE	citrate as carbon sourceCIT	D-glucuronate as carbon sourceGRT	D-galacturonate as carbon sourceGAT	2-ketogluconate as carbon source2KG	5-ketogluconate as carbon source5KG	tryptophan as carbon sourceTRY	N-acetyl-D-glucosamine as carbon sourceNAG	D-gluconate as carbon sourceGNT	phenylacetate as carbon sourcePAC	protocatechuate as carbon sourcePAT	4-hydroxybenzoate as carbon sourcepOBE	quinate as carbon sourceQAT	gentisate as carbon sourceGTE	3-hydroxybenzoate as carbon sourcemOBE	benzoate as carbon sourceBAT	3-phenylpropionate as carbon sourcePPAT	m-coumarate as carbon sourceCMT	trigonelline as carbon sourceTGE	betaine as carbon sourceBET	putrescine as carbon sourcePCE	4-aminobutyrate as carbon sourceABT	histamine as carbon sourceHIN	DL-lactate as carbon sourceLAT	caprate as carbon sourceCAP	caprylate as carbon sourceCYT	L-histidine as carbon sourceHIS	succinate as carbon sourceSUC	fumarate as carbon sourceFUM	glutarate as carbon sourceGRE	DL-glycerate as carbon sourceGYT	5-aminovalerate as carbon sourceAVT	ethanolamine as carbon sourceETN	tryptamine as carbon sourceTTN	D-glucosamine as carbon sourceGLN	itaconate as carbon sourceITA	3-hydroxybutyrate as carbon source3OBU	L-aspartate as carbon sourceAPT	L-glutamate as carbon sourceGTT	L-proline as carbon sourcePRO	D-alanine as carbon sourceDALA	L-alanine as carbon sourceLALA	L-serine as carbon sourceSER	malonate as carbon sourceMNT	propionate as carbon sourcePROP	L-tyrosine as carbon sourceTYR	2-ketoglutarate as carbon source2KT
116482	not determinedn.d.	+	+	+	+	+	-	-	-	-	+	+	-	-	-	-	-	-	-	-	+	-	-	-	-	-	-	-	-	-	-	-	+	+	-	-	-	-	-	-	-	-	-	-	-	-	-	-	+	-	-	+	-	-	+	-	-	-	-	-	+	+	-	-	-	-	-	-	-	-	-	-	-	+	+	-	-	-	-	+	-	-	-	-	-	+	-	+	-	-	+	+	+	-	+	+	-	+	+	-

Isolation, sampling and environmental information

Isolation source categories

Cat1	Cat2	Cat3
#Environmental	#Terrestrial	#Soil

Isolation

@ref	Sample type	Geographic location	Country	Country ISO 3 Code	Continent
9616	soil
67770	Soil	MO	USA	USA	North America
116482	Environment, Soil	Missouri	United States of America	USA	North America

Interaction and safety

Risk assessment

@ref	Biosafety level	Biosafety level comment
9616	1	Risk group (German classification) According to TRBA 466
19471	1	Risk group (German classification) According to TRBA 466
116482	1	Risk group (French classification) According to TRBA 466

Sequence information

16S sequences

@ref	Description	Accession	Length	NCBI tax ID
20218	Streptomyces ardus strain DSM 40527 16S ribosomal RNA gene, partial sequence	AY999843	1420	67273
20218	Streptomyces ardus gene for 16S ribosomal RNA, partial sequence, strain: JCM 4543	D44223	120	67273
20218	Streptomyces ardus gene for 16S rRNA, partial sequence, strain: NBRC 13430	AB184864	1457	67273

Genome-based predictions

Literature

Topic	Title	Authors	Journal	DOI	Year
Genetics	Factors that influence the caste ratio in a bacterial division of labour.	Avitia Dominguez LA, Yu Z, Chopra V, Viveros R, Tschowri N, Merks R, van Dijk B, Rozen D.	Philos Trans R Soc Lond B Biol Sci	10.1098/rstb.2023.0267	2025
	Bioprospecting of desert actinobacteria with special emphases on griseoviridin, mitomycin C and a new bacterial metabolite producing Streptomyces sp. PU-KB10-4.	Saleem M, Hassan A, Li F, Lu Q, Ponomareva LV, Parkin S, Sun C, Thorson JS, Shaaban KA, Sajid I.	BMC Microbiol	10.1186/s12866-023-02770-8	2023
Phylogeny	Production and biological activity of laidlomycin, anti-MRSA/VRE antibiotic from Streptomyces sp. CS684.	Yoo JC, Kim JH, Ha JW, Park NS, Sohng JK, Lee JW, Park SC, Kim MS, Seong CN	J Microbiol	2499	2007
Phylogeny	Taxonomic re-evaluation of whorl-forming Streptomyces (formerly Streptoverticillium) species by using phenotypes, DNA-DNA hybridization and sequences of gyrB, and proposal of Streptomyces luteireticuli (ex Katoh and Arai 1957) corrig., sp. nov., nom. rev.	Hatano K, Nishii T, Kasai H.	Int J Syst Evol Microbiol	10.1099/ijs.0.02238-0	2003

References

#9616	Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 40527
#19471	Wink, J.: Compendium of Actinobacteria. HZI-Helmholtz-Centre for Infection Research, Braunschweig .
#20215	Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )
#20218	Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.: StrainInfo introduces electronic passports for microorganisms.. Syst Appl Microbiol. 37: 42 - 50 2014 ( DOI 10.1016/j.syapm.2013.11.002 , PubMed 24321274 )
#33898	; Curators of the CIP;
#67770	Japan Collection of Microorganism (JCM) ; Curators of the JCM;
#68368	Automatically annotated from API 20E .
#68382	Automatically annotated from API zym .
#116482	Collection of Institut Pasteur ; Curators of the CIP; CIP 108147
#126262	A. Lissin, I. Schober, J. F. Witte, H. Lüken, A. Podstawka, J. Koblitz, B. Bunk, P. Dawyndt, P. Vandamme, P. de Vos, J. Overmann, L. C. Reimer: StrainInfo—the central database for linked microbial strain identifiers. ( DOI 10.1093/database/baaf059 )

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Streptomyces ardus (DSM 40527, ATCC 27417, CBS 731.72)

Name and taxonomic classification

Morphology

Cell morphology

Colony morphology

Multicellular morphology

Multimedia

Culture and growth conditions

Culture medium

Culture temp

Physiology and metabolism

Oxygen tolerance

Compound production

Halophily

Metabolite utilization

Metabolite production

Metabolite tests

Enzymes

API 20E

API zym

API biotype100

Isolation, sampling and environmental information

Isolation source categories

Isolation

Interaction and safety

Risk assessment

Sequence information

16S sequences

Genome-based predictions

Literature

Literature

References

BacDive

Help

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