Name: Propionibacterium ruminifibrarum JV5
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 160818

Type strain:

Species: Propionibacterium ruminifibrarum

Strain Designation: JV5

Culture col. no.: DSM 106771, TISTR 2629

Strain history: <- J.D. Vaidya, Wageningen University; JV5 <- J.D. Vaidya

NCBI tax ID(s): 1962131 (species)

SI-ID 404536

DSM 106771

Section

Propionibacterium ruminifibrarum JV5 is an aerotolerant, mesophilic, Gram-positive bacterium that was isolated from Cow rumen fibrous content.

Gram-positive
rod-shaped
aerotolerant
mesophilic
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Actinomycetota*
	Class	*Actinomycetes*
	Order	*Propionibacteriales*
	Family	*Propionibacteriaceae*
	Genus	*Propionibacterium*
	Species	**Propionibacterium ruminifibrarum**
	Full Scientific Name (LPSN)	Propionibacterium ruminifibrarum Vaidya et al. 2019

Information on morphological and physiological properties Morphology

[Ref.: #68165]	Gram stain	positive

[Ref.: #68165]	Cell length	1.0-2 µm

[Ref.: #68165]	Cell width	0.5-0.8 µm

[Ref.: #68165]	Cell shape	rod-shaped

[Ref.: #68165]	Motility	no

[Ref.: #68165]	Colony color	white
[Ref.: #68165]	Cultivation medium used	RCM

Information on culture and growth conditions Culture and growth conditions

[Ref.: #67755]

Culture medium

PYG MEDIUM (MODIFIED) (DSMZ Medium 104)

[Ref.: #67755]

Culture medium growth

[Ref.: #67755]

Culture medium link

Medium recipe at MediaDive

[Ref.: #67755]

Culture medium composition

expand / minimize

		Temperature
[Ref.: #67755]	growth	37 °C
[Ref.: #68165]	growth	35-45 °C
[Ref.: #68165]	optimum	39 °C

[Ref.: #67755]	Temperature range	mesophilic
[Ref.: #68165]	Temperature range	mesophilic

	pH	Kind of pH		pH
[Ref.: #68165]			optimum	7
[Ref.: #68165]			growth	6.5-8.5

Information on physiology and metabolism Physiology and metabolism

[Ref.: #68165]

Oxygen tolerance

aerotolerant

	Halophily	Salt	Tested relation		Salt conc.
[Ref.: #68165]		NaCl		growth	0.5-5	%(w/v)

[Ref.: #68165]

Observation

present as single cells or in cluster

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #68165]	D-lactate	+	assimilation
[Ref.: #68165]	erythritol ChEBI	+	assimilation
[Ref.: #68165]	esculin ChEBI	+	hydrolysis
[Ref.: #68165]	galactose ChEBI	+	assimilation
[Ref.: #68165]	glucose ChEBI	+	assimilation
[Ref.: #68165]	L-lactate	+	assimilation
[Ref.: #68165]	mannose ChEBI	+	assimilation
[Ref.: #68165]	myo-inositol ChEBI	+	assimilation
[Ref.: #68165]	pyruvate ChEBI	+	assimilation
[Ref.: #68165]	ribitol ChEBI	+	assimilation
[Ref.: #68165]	ribose ChEBI	+	assimilation
[Ref.: #68165]	sorbitol ChEBI	+	assimilation
	Only first 10 entries are displayed. Click here to see all.

	Metabolite production	Metabolite	Production
[Ref.: #68165]		indole ChEBI	no

	Enzyme	Enzyme activity	EC number
[Ref.: #68165]	acid phosphatase	+	3.1.3.2
[Ref.: #68165]	alkaline phosphatase	+	3.1.3.1
[Ref.: #68165]	alpha-fucosidase	-	3.2.1.51
[Ref.: #68165]	alpha-galactosidase	-	3.2.1.22
[Ref.: #68165]	alpha-glucosidase	+	3.2.1.20
[Ref.: #68165]	alpha-mannosidase	-	3.2.1.24
[Ref.: #68165]	beta-galactosidase	-	3.2.1.23
[Ref.: #68165]	beta-glucosidase	+	3.2.1.21
[Ref.: #68165]	catalase	-	1.11.1.6
[Ref.: #68165]	chymotrypsin	-	3.4.4.5
[Ref.: #68165]	cystine aminopeptidase	+	3.4.11.3
[Ref.: #68165]	esterase (C 4)	+
[Ref.: #68165]	esterase Lipase (C 8)	+
[Ref.: #68165]	gelatinase	-
[Ref.: #68165]	leucine aminopeptidase	+	3.4.1.1
[Ref.: #68165]	lipase (C 14)	+
[Ref.: #68165]	phosphoamidase	+	3.9.1.1
[Ref.: #68165]	trypsin	-	3.4.21.4
[Ref.: #68165]	urease	-	3.5.1.5
[Ref.: #68165]	valine aminopeptidase	+
	Only first 10 entries are displayed. Click here to see all.

Fatty acid profile

Metadata FA analysis

Reference

[Ref.: #68165]

Fatty acid	Percentage
C17:0	15.27
C15:1 anteiso	13.83
C18:1 w9c	10.11
C16:0	10.07
C16:0 alcohol	8.65
C15:0 iso	5.19
C18:0 iso	4.78
C15:0 anteiso	3.27
C14:0	2.61
C16:0 iso	1.52
C14:0 iso	1.04
C13:0	0.56

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #67755]	Sample type/isolated from	Cow rumen fibrous content
[Ref.: #67755]	Geographic location (country and/or sea, region)	Wageningen
[Ref.: #67755]	Country	Netherlands
[Ref.: #67755]	Country ISO 3 Code	NLD
[Ref.: #67755]	Continent	Europe

[Ref.: #68165]	Sample type/isolated from	cow rumen fibres
[Ref.: #68165]	Enrichment culture	bicarbonate-buffered anaerobic medium
[Ref.: #68165]	Enrichment culture duration	3 weeks
[Ref.: #68165]	Enrichment culture temperature	39 ̊C
* marker position based on {}

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #67755]	Propionibacterium sp. JV5 16S ribosomal RNA gene, partial sequence	MG783038	1430	ENA	1962131 tax ID

Genome sequence information:

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #68165]	Propionibacterium ruminifibrarum 17J27-24	GCA_900289195	scaffold	GenBank	1962131 tax ID

[Ref.: #68165]

GC-content

68.9 mol%

genome sequence analysis

Data predicted using genome information as a basis Genome-based predictions

	Trait	Prediction	Confidence in %	In training data
	Predictions from Diaspora project based on: Propionibacterium ruminifibrarum 17J27-24 NCBI: GCA_900289195.1
[Ref.: #69480]	flagellated	no	98.01	no
[Ref.: #69480]	gram-positive	yes	94.068	yes
[Ref.: #69480]	anaerobic	yes	53.85	yes
[Ref.: #69480]	aerobic	no	92.081	yes
[Ref.: #69480]	halophile	no	86.572	no
[Ref.: #69480]	spore-forming	no	91.728	no
[Ref.: #69480]	thermophile	no	86.712	yes
[Ref.: #69480]	glucose-util	yes	86.272	yes
[Ref.: #69480]	motile	no	93.847	yes
[Ref.: #69480]	glucose-ferment	yes	80.009	no

Availability in culture collections External links

[Ref.: #67755]

Culture collection no.

DSM 106771, TISTR 2629

[Ref.: #113026]

SI-ID 404536

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Propionibacterium ruminifibrarum sp. nov., isolated from cow rumen fibrous content.	Vaidya JD, Hornung BVH, Smidt H, Edwards JE, Plugge CM	Int J Syst Evol Microbiol	10.1099/ijsem.0.003544	2019	*

References

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#67755

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 106771

#68165

Jueeli D. Vaidya, Bastian V. H. Hornung, Hauke Smidt, Joan E. Edwards and Caroline M. Plugge: Propionibacterium ruminifibrarum sp. nov., isolated from cow rumen fibrous content. IJSEM 69: 2584 - 2590 2019 ( DOI 10.1099/ijsem.0.003544 )

#69480

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#113026

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID404536.1 )

* These data were automatically processed and therefore are not curated

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Propionibacterium ruminifibrarum 17J27-24

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