Roseovarius tolerans (DSM 108135, EL-164)

Name: Roseovarius tolerans (DSM 108135, EL-164)
Creator: BacDive
License: http://creativecommons.org/licenses/by/4.0/

BacDive ID 160494

Type strain

Strain Designation EL-164

Culture col. no. DSM 108135 EL-164

NCBI tax ID(s) 74031

Special Collections DSMZ

History <- I. Wagner-Döbler, Institute for Microbiology, TU Braunschweig, Germany; EL-164 <- M. Labrenz, Institut für Allgemeine Mikrobiologie, Univ. Kiel, Germany; EL-164

Links

Roseovarius tolerans EL-164 is a bacterium that was isolated from water, hypersaline.

genome sequence Bacteria

Name and taxonomic classification

SI-ID 397322

DSM 108135

@ref 20215

Last LPSN update 2026-02-09 11:38:54

Domain Bacteria

Phylum Pseudomonadota

Class Alphaproteobacteria

Order Rhodobacterales

Family Roseobacteraceae

Genus Roseovarius

Species Roseovarius tolerans

Full scientific name Roseovarius tolerans Labrenz et al. 1999

BacDive ID	Other strains from **Roseovarius tolerans** (2)	Type strain
13766	R. tolerans EL-172, DSM 11457, JCM 21346, CIP 106398, ... (type strain)
13767	R. tolerans EL-222, DSM 11463, CIP 106399

Morphology

Cell morphology

@ref	Gram stain	Confidence
125439	negative	94.2
125438	negative	97.262

Culture and growth conditions

Culture medium

@ref	Name	Growth	Medium link	Composition
67402	BACTO MARINE BROTH (DIFCO 2216) (DSMZ Medium 514)		Medium recipe at MediaDive	Name: BACTO MARINE BROTH (DIFCO 2216) (DSMZ Medium 514) Composition: NaCl 19.45 g/l MgCl2 5.9 g/l Bacto peptone 5.0 g/l Na2SO4 3.24 g/l CaCl2 1.8 g/l Yeast extract 1.0 g/l KCl 0.55 g/l NaHCO3 0.16 g/l Fe(III) citrate 0.1 g/l KBr 0.08 g/l SrCl2 0.034 g/l H3BO3 0.022 g/l Na2HPO4 0.008 g/l Na-silicate 0.004 g/l NaF 0.0024 g/l (NH4)NO3 0.0016 g/l Distilled water

Culture temp

@ref	Growth	Type	Temperature (°C)
67402	positive	growth	28

Physiology and metabolism

Spore formation

@ref	Spore formation	Confidence
125439		93.4

Metabolite utilization

@ref	Chebi-ID	Metabolite	Utilization activity	Kind of utilization tested
68369	17128 ChEBI	adipate	-	assimilation	from API 20NE
68369	29016 ChEBI	arginine	-	hydrolysis	from API 20NE
68369	17634 ChEBI	D-glucose	-	assimilation	from API 20NE
68369	17634 ChEBI	D-glucose	-	fermentation	from API 20NE
68369	16899 ChEBI	D-mannitol	-	assimilation	from API 20NE
68369	16024 ChEBI	D-mannose	-	assimilation	from API 20NE
68369	27689 ChEBI	decanoate	-	assimilation	from API 20NE
68369	4853 ChEBI	esculin	-	hydrolysis	from API 20NE
68369	5291 ChEBI	gelatin	-	hydrolysis	from API 20NE
68369	24265 ChEBI	gluconate	-	assimilation	from API 20NE
68369	30849 ChEBI	L-arabinose	-	assimilation	from API 20NE
68369	25115 ChEBI	malate	-	assimilation	from API 20NE
68369	17306 ChEBI	maltose	-	assimilation	from API 20NE
68369	59640 ChEBI	N-acetylglucosamine	-	assimilation	from API 20NE
68369	17632 ChEBI	nitrate	-	reduction	from API 20NE
68369	27897 ChEBI	tryptophan	-	energy source	from API 20NE
68369	16199 ChEBI	urea	-	hydrolysis	from API 20NE

Metabolite production

@ref	Chebi-ID	Metabolite	Production
68369	35581 ChEBI	indole		from API 20NE

Metabolite tests

@ref	Chebi-ID	Metabolite	Indole test
68369	35581 ChEBI	indole	-	from API 20NE

Enzymes

@ref	Value	Activity	Ec
68382	acid phosphatase	-	3.1.3.2	from API zym
68382	alkaline phosphatase	-	3.1.3.1	from API zym
68382	alpha-chymotrypsin	-	3.4.21.1	from API zym
68382	alpha-fucosidase	-	3.2.1.51	from API zym
68382	alpha-galactosidase	-	3.2.1.22	from API zym
68382	alpha-glucosidase	-	3.2.1.20	from API zym
68382	alpha-mannosidase	-	3.2.1.24	from API zym
68369	arginine dihydrolase	-	3.5.3.6	from API 20NE
68382	beta-galactosidase	-	3.2.1.23	from API zym
68382	beta-glucosidase	-	3.2.1.21	from API zym
68369	beta-glucosidase	-	3.2.1.21	from API 20NE
68382	beta-glucuronidase	-	3.2.1.31	from API zym
68382	cystine arylamidase	-	3.4.11.3	from API zym
68382	esterase (C 4)	-		from API zym
68369	gelatinase	-		from API 20NE
68382	leucine arylamidase	+	3.4.11.1	from API zym
68382	lipase (C 14)	-		from API zym
68382	N-acetyl-beta-glucosaminidase	-	3.2.1.52	from API zym
68382	naphthol-AS-BI-phosphohydrolase	-		from API zym
68382	trypsin	-	3.4.21.4	from API zym
68369	urease	-	3.5.1.5	from API 20NE

API zym

@ref	Control	Alkaline phosphatase	Esterase (C 4)	2-naphtyl caprylateEsterase Lipase (C 8)	Lipase (C 14)	L-leucyl-2-naphthylamideLeucine arylamidase	L-valyl-2-naphthylamideValine arylamidase	L-cystyl-2-naphthylamideCystine arylamidase	Trypsin	alpha- Chymotrypsin	Acid phosphatase	Naphthol-AS-BI-phosphateNaphthol-AS-BI-phosphohydrolase	alpha- Galactosidase	beta- Galactosidase	beta- Glucuronidase	alpha- Glucosidase	beta- Glucosidase	N-acetyl-beta- glucosaminidase	alpha- Mannosidase	alpha- Fucosidase
67402	-	-	-	+/-	-	+	+/-	-	-	-	-	-	-	-	-	-	-	-	-	-

API 20NE

@ref	Reduction of nitratesNO3	TRP	GLU_ Ferm	ADH (Arg)	URE	ESC	GEL	PNPG	GLU_ Assim	ARA	MNE	MAN	NAG	MAL	GNT	CAP	ADI	MLT	CIT	PAC	OX
67402	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	not determinedn.d.

Isolation, sampling and environmental information

Isolation

@ref	Sample type	Geographic location	Country	Country ISO 3 Code	Continent	Latitude	Longitude
67402	water, hypersaline	Ekho Lake, Vestfold Hills (68° 31' 16.0 '' S, 78° 16' 12.0'' E)	Antarctica	ATA	Australia and Oceania	-68.5211	78.27 -68.5211/78.27

Interaction and safety

Risk assessment

@ref	Biosafety level	Biosafety level comment
67402	1	Risk group (German classification)

Sequence information

Genome sequences

@ref	Description	Assembly level	INSDC accession	IMG accession	NCBI tax ID	Score
66792	ASM2888539v1 assembly for Roseovarius tolerans EL-164	complete	GCA_028885395		74031	88.93
66792	ASM124771v1 assembly for Roseovarius tolerans EL-164	contig	GCA_001247715	2740892180	74031	49.89

Genome-based predictions

Predictions

Predictions from Deep Learning for Genome Sequence Data. R package version 0.3.1 based on: Roseovarius tolerans EL-164
NCBI: GCA_001247715

@ref	Trait	Model	Prediction	Confidence in %	In training data
125439	spore_formation	BacteriaNetⓘ	no	93.40	no
125439	motility	BacteriaNetⓘ	yes	61.40	no
125439	gram_stain	BacteriaNetⓘ	negative	94.20	no
125439	oxygen_tolerance	BacteriaNetⓘ	obligate aerobe	82.70	no

Predictions from bacterial phenotypic traits through improved machine learning using high-quality, curated datasets based on: Roseovarius tolerans EL-164
NCBI: GCA_028885395.1

@ref	Trait	Model	Prediction	Confidence in %	In training data
125438	gram-positive	gram-positiveⓘ	no	97.26	no
125438	anaerobic	anaerobicⓘ	no	90.22	no
125438	aerobic	aerobicⓘ	yes	85.24	no
125438	spore-forming	spore-formingⓘ	no	89.12	no
125438	thermophilic	thermophileⓘ	no	96.44	yes
125438	flagellated	motile2+ⓘ	no	72.76	no

Literature

Topic	Title	Authors	Journal	DOI	Year
	Chemical quantification of N-acyl alanine methyl ester (NAME) production and impact on temporal gene expression patterns in Roseovarius tolerans EL-164.	Leinberger J, Koteska D, Boldt J, Petersen J, Shivaramu S, Tomasch J, Schulz S, Brinkhoff T.	BMC Microbiol	10.1186/s12866-024-03624-7	2024
	N-Acylated amino acid methyl esters from marine Roseobacter group bacteria.	Bruns H, Ziesche L, Taniwal NK, Wolter L, Brinkhoff T, Herrmann J, Muller R, Schulz S	Beilstein J Org Chem	10.3762/bjoc.14.276	2018
Pathogenicity	Oxygenated N-Acyl Alanine Methyl Esters (NAMEs) from the Marine Bacterium Roseovarius tolerans EL-164.	Bruns H, Herrmann J, Muller R, Wang H, Wagner Dobler I, Schulz S	J Nat Prod	10.1021/acs.jnatprod.7b00757	2017
Genetics	Draft Genome Sequence of Roseovarius tolerans EL-164, a Producer of N-Acylated Alanine Methyl Esters and N-Acylhomoserine Lactones.	Voget S, Bruns H, Wagner-Dobler I, Schulz S, Daniel R	Genome Announc	10.1128/genomeA.01096-15	2015

References

#20215	Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )
#66792	Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .
#67402	Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 108135
#68369	Automatically annotated from API 20NE .
#68382	Automatically annotated from API zym .
#125438	Julia Koblitz, Lorenz Christian Reimer, Rüdiger Pukall, Jörg Overmann: Predicting bacterial phenotypic traits through improved machine learning using high-quality, curated datasets. 2024 ( DOI 10.1101/2024.08.12.607695 )
#125439	Philipp Münch, René Mreches, Martin Binder, Hüseyin Anil Gündüz, Xiao-Yin To, Alice McHardy: deepG: Deep Learning for Genome Sequence Data. R package version 0.3.1 .
#126262	A. Lissin, I. Schober, J. F. Witte, H. Lüken, A. Podstawka, J. Koblitz, B. Bunk, P. Dawyndt, P. Vandamme, P. de Vos, J. Overmann, L. C. Reimer: StrainInfo—the central database for linked microbial strain identifiers. ( DOI 10.1093/database/baaf059 )

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Roseovarius tolerans (DSM 108135, EL-164)

Name and taxonomic classification

Morphology

Cell morphology

Culture and growth conditions

Culture medium

Culture temp

Physiology and metabolism

Spore formation

Metabolite utilization

Metabolite production

Metabolite tests

Enzymes

API zym

API 20NE

Isolation, sampling and environmental information

Isolation

Interaction and safety

Risk assessment

Sequence information

Genome sequences

Genome browser: GCA_028885395

Genome-based predictions

Predictions

Literature

Literature

References

BacDive

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