Methanofollis fontis (DSM 107935, NBRC 113164, BCRC AR10052)

Name: Methanofollis fontis (DSM 107935, NBRC 113164, BCRC AR10052)
Creator: BacDive
License: http://creativecommons.org/licenses/by/4.0/

BacDive ID 160432

Type strain

Strain Designation FWC-SCC2

Culture col. no. DSM 107935 NBRC 113164 BCRC AR10052 FWC-SCC2

NCBI tax ID(s) 2052832

Special Collections DSMZ

History <- M.-C. Lai, National Chung Hsing University, Taichung, Taiwan; FWC-SCC2 <- S.-C. Chen; {2017}

Links

Methanofollis fontis FWC-SCC2 is an archaeon that was isolated from Sub-seafloor sediment from Four Way Closure Ridge offshore southwestern Taiwan.

genome sequence 16S sequence Archaea

Name and taxonomic classification

SI-ID 401640

NBRC 113164,DSM 107935

@ref 20215

Last LPSN update 2026-02-09 11:38:53

Domain Archaea

Phylum Methanobacteriota

Class "Methanomicrobia"

Order Methanomicrobiales

Family Methanomicrobiaceae

Genus Methanofollis

Species Methanofollis fontis

Full scientific name Methanofollis fontis Chen et al. 2020

Morphology

Cell morphology

@ref	Gram stain	Confidence
125439	negative	96.1

Culture and growth conditions

Culture medium

@ref	Name	Growth	Medium link	Composition
67340	METHANOCALCULUS MB MEDIUM (DSMZ Medium 924)		Medium recipe at MediaDive	Name: METHANOCALCULUS MB MEDIUM (DSMZ Medium 924) Composition: Na-formate 6.72601 g/l NaHCO3 3.95648 g/l Trypticase peptone 1.97824 g/l Yeast extract 1.97824 g/l Na-acetate 1.58259 g/l MgCl2 x 6 H2O 0.98912 g/l NH4Cl 0.98912 g/l KCl 0.49456 g/l K2HPO4 0.395648 g/l Na2S x 9 H2O 0.24728 g/l L-Cysteine HCl x H2O 0.24728 g/l MgSO4 x 7 H2O 0.0296736 g/l Nitrilotriacetic acid 0.0148368 g/l NaCl 0.0098912 g/l MnSO4 x H2O 0.0049456 g/l CoSO4 x 7 H2O 0.00178042 g/l ZnSO4 x 7 H2O 0.00178042 g/l CaCl2 x 2 H2O 0.00098912 g/l FeSO4 x 7 H2O 0.00098912 g/l Sodium resazurin 0.00049456 g/l NiCl2 x 6 H2O 0.000296736 g/l AlK(SO4)2 x 12 H2O 0.000197824 g/l CuSO4 x 5 H2O 9.8912e-05 g/l H3BO3 9.8912e-05 g/l Pyridoxine hydrochloride 9.8912e-05 g/l Na2MoO4 x 2 H2O 9.8912e-05 g/l p-Aminobenzoic acid 4.9456e-05 g/l (DL)-alpha-Lipoic acid 4.9456e-05 g/l Calcium D-(+)-pantothenate 4.9456e-05 g/l Nicotinic acid 4.9456e-05 g/l Riboflavin 4.9456e-05 g/l Thiamine HCl 4.9456e-05 g/l Folic acid 1.97824e-05 g/l Biotin 1.97824e-05 g/l Na2WO4 x 2 H2O 3.95648e-06 g/l Na2SeO3 x 5 H2O 2.96736e-06 g/l Vitamin B12 9.8912e-07 g/l Distilled water

Culture temp

@ref	Growth	Type	Temperature (°C)
67340	positive	growth	37

Physiology and metabolism

Oxygen tolerance

@ref	Oxygen tolerance	Confidence
125439	anaerobe	99.7

Spore formation

@ref	Spore formation	Confidence
125439		97.1

Isolation, sampling and environmental information

Isolation

@ref	Sample type	Geographic location	Country	Country ISO 3 Code	Continent
67340	Sub-seafloor sediment from Four Way Closure Ridge offshore southwestern Taiwan	Four Way Closure Ridge offshore southwestern Taiwan	Taiwan, Province of China	TWN	Asia

Interaction and safety

Sequence information

Genome sequences

@ref	Description	Assembly level	INSDC accession	BV-BRC accession	NCBI tax ID	Score
66792	ASM429718v1 assembly for Methanofollis fontis FWC-SCC2	contig	GCA_004297185	2052832.3	2052832	71.2

16S sequences

@ref	Description	Accession	Length	Database	NCBI tax ID
67340	Methanofollis fontis strain FWC-SCC2 16S ribosomal RNA gene, partial sequence	MG437305	1397		2052832

GC content

@ref	GC-content (mol%)	Method
67340	59.5	sequence analysis

Genome-based predictions

Predictions

Predictions from Deep Learning for Genome Sequence Data. R package version 0.3.1 based on: Methanofollis fontis FWC-SCC2
NCBI: GCA_004297185

@ref	Trait	Model	Prediction	Confidence in %	In training data
125439	spore_formation	BacteriaNetⓘ	no	97.10	no
125439	motility	BacteriaNetⓘ	no	64.10	no
125439	gram_stain	BacteriaNetⓘ	negative	96.10	no
125439	oxygen_tolerance	BacteriaNetⓘ	anaerobe	99.70	no

Predictions from bacterial phenotypic traits through improved machine learning using high-quality, curated datasets based on: Methanofollis fontis FWC-SCC2
NCBI: GCA_004297185.1

@ref	Trait	Model	Prediction	Confidence in %	In training data
125438	gram-positive	gram-positiveⓘ	no	70.64	no
125438	anaerobic	anaerobicⓘ	yes	87.88	no
125438	aerobic	aerobicⓘ	no	88.67	no
125438	spore-forming	spore-formingⓘ	no	88.02	no
125438	thermophilic	thermophileⓘ	no	82.43	no
125438	flagellated	motile2+ⓘ	no	86.99	no

Literature

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Methanofollis fontis sp. nov., a methanogen isolated from marine sediment near a cold seep at Four-Way Closure Ridge offshore southwestern Taiwan.	Chen SC, Teng NH, Lin YS, Lai MC, Chen HH, Wang CC	Int J Syst Evol Microbiol	10.1099/ijsem.0.004440	2020

References

#20215	Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )
#66792	Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .
#67340	Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 107935
#125438	Julia Koblitz, Lorenz Christian Reimer, Rüdiger Pukall, Jörg Overmann: Predicting bacterial phenotypic traits through improved machine learning using high-quality, curated datasets. 2024 ( DOI 10.1101/2024.08.12.607695 )
#125439	Philipp Münch, René Mreches, Martin Binder, Hüseyin Anil Gündüz, Xiao-Yin To, Alice McHardy: deepG: Deep Learning for Genome Sequence Data. R package version 0.3.1 .
#126262	A. Lissin, I. Schober, J. F. Witte, H. Lüken, A. Podstawka, J. Koblitz, B. Bunk, P. Dawyndt, P. Vandamme, P. de Vos, J. Overmann, L. C. Reimer: StrainInfo—the central database for linked microbial strain identifiers. ( DOI 10.1093/database/baaf059 )

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Methanofollis fontis (DSM 107935, NBRC 113164, BCRC AR10052)

Name and taxonomic classification

Morphology

Cell morphology

Culture and growth conditions

Culture medium

Culture temp

Physiology and metabolism

Oxygen tolerance

Spore formation

Isolation, sampling and environmental information

Isolation

Interaction and safety

Sequence information

Genome sequences

Genome browser: GCA_004297185

16S sequences

GC content

Genome-based predictions

Predictions

Literature

Literature

References

BacDive

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