Name: Alkaliphilus pronyensis LacV
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 160373

Type strain:

Species: Alkaliphilus pronyensis

Strain Designation: LacV

Culture col. no.: DSM 100017, JCM 30644

Strain history: A. Postec; Mediterr. Inst. of Oceanogr., France; LacV.

NCBI tax ID(s): 1482732 (species)

SI-ID 398683

DSM 100017

Section

Alkaliphilus pronyensis LacV is an anaerobe, organoheterotroph, spore-forming bacterium that was isolated from alkaline hydrothermal vent.

motile
spore-forming
anaerobe
mesophilic
organoheterotroph
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Bacillota*
	Class	*Clostridia*
	Order	*Eubacteriales*
	Family	*Clostridiaceae*
	Genus	*Alkaliphilus*
	Species	**Alkaliphilus pronyensis**
	Full Scientific Name (LPSN)	Alkaliphilus pronyensis Postec et al. 2022

Information on morphological and physiological properties Morphology

[Ref.: #69644]	Gram stain	positive
[Ref.: #69480]	Gram stain	positive Confidence in %100

[Ref.: #69644]	Cell length	2.9-4.5 µm

[Ref.: #69644]	Cell width	0.4-0.6 µm

[Ref.: #69644]	Cell shape	rod-shaped

[Ref.: #69644]	Motility	yes
[Ref.: #69480]	Motility	yes Confidence in %90.474

Information on culture and growth conditions Culture and growth conditions

[Ref.: #67279]

Culture medium

ANAEROBACILLUS MEDIUM (DSMZ Medium 898a)

[Ref.: #67279]

Culture medium growth

[Ref.: #67279]

Culture medium link

Medium recipe at MediaDive

[Ref.: #67279]

Culture medium composition

expand / minimize

[Ref.: #69644]

Culture medium

basal medium

[Ref.: #69644]

Culture medium growth

		Temperature
[Ref.: #67279]	growth	30 °C
[Ref.: #67770]	growth	35 °C
[Ref.: #69644]	growth	20-40 °C
[Ref.: #69644]	optimum	30 °C

[Ref.: #67279]	Temperature range	mesophilic
[Ref.: #67770]	Temperature range	mesophilic
[Ref.: #69644]	Temperature range	mesophilic

	pH	Kind of pH		pH
[Ref.: #69644]			optimum	8.7
[Ref.: #69644]			growth	8.2-10.0

Information on physiology and metabolism Physiology and metabolism

[Ref.: #69644]	Oxygen tolerance	anaerobe
[Ref.: #69480]	Oxygen tolerance	anaerobe Confidence in %99.614

[Ref.: #69644]

Nutrition type

organoheterotroph

[Ref.: #69644]	Spore description	terminal endospore-forming
[Ref.: #69644]	Type of spore	endospore
[Ref.: #69644]	Ability of spore formation	yes
[Ref.: #69480]	Ability of spore formation	yes Confidence in %99.448
[Ref.: #69481]	Ability of spore formation	yes Confidence in %100

	Halophily	Salt	Tested relation		Salt conc.
[Ref.: #69644]		NaCl		growth	0-8	%(w/v)
[Ref.: #69644]		NaCl		optimum	2.5	%(w/v)

[Ref.: #69644]	Observation	Cells occur singly or in pairs
[Ref.: #69644]	Observation	presence of a jagged peptidoglycan and a multilayered cell wall
[Ref.: #69644]	Observation	presence of an outer membrane

[Ref.: #69644]	Name of tolerated compound	Oxgen in the gas phase
[Ref.: #69644]	Tolerance percentage	0.1

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #69644]	acetate ChEBI	-	required for growth
[Ref.: #69644]	amorphous iron (iii) oxide	-	electron acceptor
[Ref.: #69644]	arabinose ChEBI	-	required for growth
[Ref.: #69644]	butyrate ChEBI	-	required for growth
[Ref.: #69644]	casamino acids	+	fermentation
[Ref.: #69644]	cellobiose ChEBI	-	required for growth
[Ref.: #69644]	citrate ChEBI	-	required for growth
[Ref.: #69644]	crotonate ChEBI	+	assimilation
[Ref.: #69644]	elemental sulfur ChEBI	-	electron acceptor
[Ref.: #69644]	ethylenediaminetetraacetatoferrate ChEBI	-	electron acceptor
[Ref.: #69644]	ferric citrate ChEBI	-	electron acceptor
[Ref.: #69644]	formate ChEBI	-	required for growth
[Ref.: #69644]	fructose ChEBI	-	assimilation
[Ref.: #69644]	fumarate ChEBI	-	electron acceptor
[Ref.: #69644]	fumarate ChEBI	-	required for growth
[Ref.: #69644]	galactose ChEBI	-	assimilation
[Ref.: #69644]	glucose ChEBI	-	assimilation
[Ref.: #69644]	glycerol ChEBI	-	required for growth
[Ref.: #69644]	goethite	-	electron acceptor
[Ref.: #69644]	lactate ChEBI	-	required for growth
[Ref.: #69644]	lactose ChEBI	-	assimilation
[Ref.: #69644]	malate ChEBI	-	required for growth
[Ref.: #69644]	maltose ChEBI	-	assimilation
[Ref.: #69644]	mannose ChEBI	-	assimilation
[Ref.: #69644]	melibiose ChEBI	-	required for growth
[Ref.: #69644]	nitrate ChEBI	-	electron acceptor
[Ref.: #69644]	nitrite ChEBI	-	electron acceptor
[Ref.: #69644]	peptone	+	fermentation
[Ref.: #69644]	pyruvate ChEBI	-	required for growth
[Ref.: #69644]	raffinose ChEBI	-	required for growth
[Ref.: #69644]	ribose ChEBI	-	assimilation
[Ref.: #69644]	ribose ChEBI	-	required for growth
[Ref.: #69644]	succinate ChEBI	-	required for growth
[Ref.: #69644]	sucrose ChEBI	-	assimilation
[Ref.: #69644]	sulfate ChEBI	-	electron acceptor
[Ref.: #69644]	sulfite ChEBI	-	electron acceptor
[Ref.: #69644]	tartrate ChEBI	-	required for growth
[Ref.: #69644]	thiosulfate ChEBI	-	electron acceptor
[Ref.: #69644]	trehalose ChEBI	-	assimilation
[Ref.: #69644]	xylose ChEBI	-	assimilation
[Ref.: #69644]	yeast extract	+	fermentation
	Only first 10 entries are displayed. Click here to see all.

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #67279]	Sample type/isolated from	alkaline hydrothermal vent
[Ref.: #67279]	Geographic location (country and/or sea, region)	New Caledonia, Prony Bay
[Ref.: #67279]	Country	France
[Ref.: #67279]	Country ISO 3 Code	FRA
[Ref.: #67279]	Continent	Europe

[Ref.: #67770]	Sample type/isolated from	Prony Bay Hydrothermal Field located in the south of New Caledonia

[Ref.: #69644]	Sample type/isolated from	Carbonate chimney in Prony hydrothermal field
[Ref.: #69644]	Sampling date	2011-11-01
[Ref.: #69644]	Geographic location (country and/or sea, region)	Prony Bay Hydrothermal Field
[Ref.: #69644]	Country	New Caledonia
[Ref.: #69644]	Country ISO 3 Code	NCL
[Ref.: #69644]	Continent	Australia and Oceania
[Ref.: #69644]	Geographic location	-22.3667°/166.8830°
[Ref.: #69644]	Enrichment culture	basal medium
[Ref.: #69644]	Enrichment culture duration	2 weeks
[Ref.: #69644]	Enrichment culture temperature	30 ̊C
* marker position based on {}

[Ref.: #69479]

Global distribution of 16S sequence KJ159207 (>99% sequence identity) for Alkaliphilus from Microbeatlas

Aquatic Samples	5
Soil Samples	2
Animal Samples	3
Plant Samples
Total Samples	10

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #67279]	Alkaliphilus sp. LacV 16S ribosomal RNA gene, partial sequence	KJ159207	1400	ENA	1482732 tax ID

Genome sequence information:

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #67770]	Alkaliphilus pronyensis LacV	GCA_009017275	contig	GenBank	1482732 tax ID
[Ref.: #69644]	Whole genome shotgun	WBZC00000000		GenBank	1482732 tax ID
[Ref.: #66792]	Alkaliphilus sp. LacV	1482732.3	wgs	PATRIC	1482732 tax ID	*

[Ref.: #67279]	GC-content	33.9 mol%	sequence analysis
[Ref.: #67770]	GC-content	33.9 mol%	genome sequence analysis
[Ref.: #67770]	GC-content	36.3 mol%	high performance liquid chromatography (HPLC)
[Ref.: #69644]	GC-content	33.87 mol%	high performance liquid chromatography (HPLC)

Data predicted using genome information as a basis Genome-based predictions

	Trait	Prediction	Confidence in %	In training data
	Predictions from GenomeNet Sporulation v. 1 based on: Alkaliphilus sp. LacV PATRIC: 1482732.3
[Ref.: #69481]	spore-forming	yes	100	no

	Trait	Prediction	Confidence in %	In training data
	Predictions from Diaspora project based on: Alkaliphilus sp. LacV PATRIC: 1482732.3
[Ref.: #69480]	motile	yes	83.744	no
[Ref.: #69480]	gram-positive	yes	61.357	no
[Ref.: #69480]	anaerobic	yes	99.075	no
[Ref.: #69480]	aerobic	no	96.895	no
[Ref.: #69480]	halophile	no	83.2	no
[Ref.: #69480]	spore-forming	yes	90.959	no
[Ref.: #69480]	glucose-util	yes	85.02	no
[Ref.: #69480]	flagellated	no	76.227	no
[Ref.: #69480]	thermophile	no	96.957	yes
[Ref.: #69480]	glucose-ferment	no	68.704	no

Availability in culture collections External links

[Ref.: #67279]

Culture collection no.

DSM 100017, JCM 30644

[Ref.: #112622]

SI-ID 398683

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Alkaliphilus serpentinus sp. nov. and Alkaliphilus pronyensis sp. nov., two novel anaerobic alkaliphilic species isolated from the serpentinite-hosted Prony Bay Hydrothermal Field (New Caledonia).	Postec A, Quemeneur M, Lecoeuvre A, Chabert N, Joseph M, Erauso G	Syst Appl Microbiol	10.1016/j.syapm.2020.126175	2020	*

References

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#67279

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 100017

#67770 Japan Collection of Microorganism (JCM) ; Curators of the JCM;

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

#69480

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#69481

Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch: Predictions based on the model GenomeNet Sporulation v. 1 . ( DOI 10.21203/rs.3.rs-2527258/v1 )

#69644

Anne Postec, Marianne Quéméneur, Aurélien Lecoeuvre, Nicolas Chabert, Manon Joseph, Gaël Erauso: Alkaliphilus serpentinus sp. nov. and Alkaliphilus pronyensis sp. nov., two novel anaerobic alkaliphilic species isolated from the serpentinite-hosted Prony Bay Hydrothermal Field (New Caledonia). Syst Appl Microbiol. 44: 2021 ( DOI 10.1016/j.syapm.2020.126175 )

#112622

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID398683.1 )

* These data were automatically processed and therefore are not curated

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Alkaliphilus sp. LacV

Alkaliphilus sp. LacV

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