Name: Occultella glacieicola T3246-1
Creator: BacDive
License: https://creativecommons.org/licenses/by/4.0/

Strain identifier

BacDive ID: 160327

Type strain:

Species: Occultella glacieicola

Strain Designation: T3246-1

Culture col. no.: DSM 110499, JCM 32923, CGMCC 1.13532, CGMCC 1.13535

Strain history: Y. Liu; CAS, China; T3246-1.

NCBI tax ID(s): 2518684 (species)

SI-ID 400396

DSM 110499

Special Collections

Section

When using BacDive for research please cite our paper

Occultella glacieicola T3246-1 is an aerobe, psychrophilic, Gram-positive bacterium that forms circular colonies and was isolated from ice core.

colony-forming
Gram-positive
motile
rod-shaped
aerobe
psychrophilic
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	09-12-2024 (DD-MM-YYYY)
	Domain	*Bacteria*
	Phylum	*Actinomycetota*
	Class	*Actinomycetes*
	Order	*Micrococcales*
	Family	*Ruaniaceae*
	Genus	*Occultella*
	Species	**Occultella glacieicola**
	Full Scientific Name (LPSN)	Occultella glacieicola (Wang et al. 2019) Schumann et al. 2021

Synonym

Haloactinobacterium glacieicola

Information on morphological and physiological properties Morphology

[Ref.: #68213]	Gram stain	positive

[Ref.: #68213]	Cell length	1.7 µm

[Ref.: #68213]	Cell width	0.6 µm

[Ref.: #68213]	Cell shape	rod-shaped

[Ref.: #68213]	Motility	yes

[Ref.: #68213]	Colony color	yellow
[Ref.: #68213]	Colony shape	circular
[Ref.: #68213]	Incubation period	2-3 days
[Ref.: #68213]	Cultivation medium used	Reasoner's 2A agar (R2A)

Information on culture and growth conditions Culture and growth conditions

[Ref.: #67230]

Culture medium

TRYPTICASE SOY YEAST EXTRACT MEDIUM (DSMZ Medium 92)

[Ref.: #67230]

Culture medium growth

[Ref.: #67230]

Culture medium link

Medium recipe at MediaDive

[Ref.: #67230]

Culture medium composition

expand / minimize

[Ref.: #67230]

Culture medium

BACTO MARINE BROTH (DIFCO 2216) (DSMZ Medium 514)

[Ref.: #67230]

Culture medium growth

[Ref.: #67230]

Culture medium link

Medium recipe at MediaDive

[Ref.: #67230]

Culture medium composition

expand / minimize

		Temperature
[Ref.: #67230]	growth	20 °C
[Ref.: #67770]	growth	20 °C
[Ref.: #68213]	growth	10-30 °C
[Ref.: #68213]	optimum	20 °C

	pH	Kind of pH		pH
[Ref.: #68213]			optimum	7-8
[Ref.: #68213]			growth	6-10

Information on physiology and metabolism Physiology and metabolism

[Ref.: #68213]

Oxygen tolerance

aerobe

	Halophily	Salt	Tested relation		Salt conc.
[Ref.: #68213]		NaCl		growth	1-5	%(w/v)
[Ref.: #68213]		NaCl		optimum	3	%(w/v)

[Ref.: #67770]

Observation

quinones: MK-8

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #68213]	amygdalin ChEBI	-	builds acid from
[Ref.: #68213]	arabinose ChEBI	-	builds acid from
[Ref.: #68213]	arbutin ChEBI	-	builds acid from
[Ref.: #68213]	cellobiose ChEBI	+	builds acid from
[Ref.: #68213]	D-arabitol ChEBI	-	builds acid from
[Ref.: #68213]	D-fructose ChEBI	-	builds acid from
[Ref.: #68213]	D-fucose ChEBI	-	builds acid from
[Ref.: #68213]	D-galactose ChEBI	+	builds acid from
[Ref.: #68213]	D-glucose ChEBI	+	builds acid from
[Ref.: #68213]	D-lyxose ChEBI	+	builds acid from
[Ref.: #68213]	D-mannitol ChEBI	-	builds acid from
[Ref.: #68213]	D-mannose ChEBI	+	builds acid from
[Ref.: #68213]	D-ribose ChEBI	-	builds acid from
[Ref.: #68213]	D-sorbitol ChEBI	-	builds acid from
[Ref.: #68213]	D-tagatose ChEBI	+	builds acid from
[Ref.: #68213]	D-xylose ChEBI	-	builds acid from
[Ref.: #68213]	erythritol ChEBI	+	builds acid from
[Ref.: #68213]	esculin ChEBI	+	hydrolysis
[Ref.: #68213]	galactitol ChEBI	-	builds acid from
[Ref.: #68213]	gelatin ChEBI	+	hydrolysis
[Ref.: #68213]	gentiobiose ChEBI	-	builds acid from
[Ref.: #68213]	glycerol ChEBI	+	builds acid from
[Ref.: #68213]	glycogen ChEBI	-	builds acid from
[Ref.: #68213]	inulin ChEBI	-	builds acid from
[Ref.: #68213]	L-arabitol ChEBI	-	builds acid from
[Ref.: #68213]	L-fucose ChEBI	-	builds acid from
[Ref.: #68213]	L-rhamnose ChEBI	-	builds acid from
[Ref.: #68213]	L-sorbose ChEBI	-	builds acid from
[Ref.: #68213]	L-xylose ChEBI	-	builds acid from
[Ref.: #68213]	lactose ChEBI	-	builds acid from
[Ref.: #68213]	maltose ChEBI	-	builds acid from
[Ref.: #68213]	melezitose ChEBI	-	builds acid from
[Ref.: #68213]	melibiose ChEBI	-	builds acid from
[Ref.: #68213]	methyl alpha-D-glucopyranoside ChEBI	-	builds acid from
[Ref.: #68213]	methyl alpha-D-mannoside ChEBI	-	builds acid from
[Ref.: #68213]	methyl beta-D-xylopyranoside ChEBI	-	builds acid from
[Ref.: #68213]	myo-inositol ChEBI	-	builds acid from
[Ref.: #68213]	N-acetylglucosamine ChEBI	+	builds acid from
[Ref.: #68213]	nitrate ChEBI	+	reduction
[Ref.: #68213]	potassium 2-dehydro-D-gluconate	-	builds acid from
[Ref.: #68213]	potassium 5-dehydro-D-gluconate	-	builds acid from
[Ref.: #68213]	potassium gluconate ChEBI	-	builds acid from
[Ref.: #68213]	raffinose ChEBI	-	builds acid from
[Ref.: #68213]	ribitol ChEBI	-	builds acid from
[Ref.: #68213]	starch ChEBI	-	builds acid from
[Ref.: #68213]	sucrose ChEBI	-	builds acid from
[Ref.: #68213]	trehalose ChEBI	-	builds acid from
[Ref.: #68213]	turanose ChEBI	+	builds acid from
[Ref.: #68213]	xylitol ChEBI	-	builds acid from
	Only first 10 entries are displayed. Click here to see all.

	Metabolite production	Metabolite	Production
[Ref.: #68213]		hydrogen sulfide ChEBI	yes
[Ref.: #68213]		indole ChEBI	no

	Enzyme	Enzyme activity	EC number
[Ref.: #68213]	6-phospho-beta-galactosidase	-	3.2.1.85
[Ref.: #68213]	acid phosphatase	-	3.1.3.2
[Ref.: #68213]	alkaline phosphatase	-	3.1.3.1
[Ref.: #68213]	alpha-galactosidase	-	3.2.1.22
[Ref.: #68213]	alpha-glucosidase	-	3.2.1.20
[Ref.: #68213]	alpha-mannosidase	-	3.2.1.24
[Ref.: #68213]	beta-D-fucosidase	-	3.2.1.38
[Ref.: #68213]	beta-glucosidase	-	3.2.1.21
[Ref.: #68213]	beta-glucuronidase	-	3.2.1.31
[Ref.: #68213]	catalase	+	1.11.1.6
[Ref.: #68213]	chymotrypsin	-	3.4.4.5
[Ref.: #68213]	cystine arylamidase	-	3.4.11.3
[Ref.: #68213]	cytochrome oxidase	+	1.9.3.1
[Ref.: #68213]	esterase (C 4)	+
[Ref.: #68213]	esterase Lipase (C 8)	+
[Ref.: #68213]	leucine arylamidase	-	3.4.11.1
[Ref.: #68213]	lipase (C 14)	-
[Ref.: #68213]	N-acetyl-beta-glucosaminidase	-	3.2.1.52
[Ref.: #68213]	naphthol-AS-BI-phosphohydrolase	+
[Ref.: #68213]	trypsin	+	3.4.21.4
[Ref.: #68213]	valine arylamidase	-
	Only first 10 entries are displayed. Click here to see all.

Fatty acid profile

Metadata FA analysis

Reference

[Ref.: #68213]

Fatty acid	Percentage
C15:0 anteiso	57.7
C14:0 iso	16.6
C14:0	4.9
C16:0 iso	4.1
C15:0 iso	3.1
C17:1 w8c	2.5
C16:0	2.2
C18:0	1.7
C17:0 anteiso	1.2

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #67230]	Sample type/isolated from	ice core
[Ref.: #67230]	Geographic location (country and/or sea, region)	Hariqin Glacier
[Ref.: #67230]	Country	China
[Ref.: #67230]	Country ISO 3 Code	CHN
[Ref.: #67230]	Continent	Asia

[Ref.: #67770]	Sample type/isolated from	Ice core in Hariqin Glacier
[Ref.: #67770]	Geographic location (country and/or sea, region)	Tibet
[Ref.: #67770]	Country	China
[Ref.: #67770]	Country ISO 3 Code	CHN
[Ref.: #67770]	Continent	Asia

[Ref.: #68213]	Sample type/isolated from	ice core
[Ref.: #68213]	Sampling date	2015-05-01
[Ref.: #68213]	Geographic location (country and/or sea, region)	Hariqin Glacier, Tibet
[Ref.: #68213]	Country	China
[Ref.: #68213]	Country ISO 3 Code	CHN
[Ref.: #68213]	Continent	Asia
[Ref.: #68213]	Geographic location	33.1300°/92.1200°
[Ref.: #68213]	Enrichment culture	R2A agar
[Ref.: #68213]	Enrichment culture duration	30 days
[Ref.: #68213]	Enrichment culture temperature	4 ̊C
* marker position based on {}

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #68213]	Occultella glacieicola strain T3246-1 16S ribosomal RNA gene, partial sequence	MG182697	1393	GenBank ENA	2518684 tax ID

Genome sequence information:

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #68213]	Occultella glacieicola T3246-1	GCA_004353825	contig	GenBank ENA	2518684 tax ID

[Ref.: #68213]

GC-content

71.4 mol%

genome sequence analysis

Data predicted using genome information as a basis Genome-based predictions

	Model	Trait	Prediction	Confidence in %	In training data
	Predictions from bacterial phenotypic traits through improved machine learning using high-quality, curated datasets based on: Occultella glacieicola T3246-1 NCBI: GCA_004353825.1
[Ref.: #125438]	gram-positive	gram-positiveⓘ	yes	86.766	no
[Ref.: #125438]	anaerobic	anaerobicⓘ	no	93.383	yes
[Ref.: #125438]	aerobic	aerobicⓘ	yes	81.883	yes
[Ref.: #125438]	spore-forming	spore-formingⓘ	no	67.516	no
[Ref.: #125438]	thermophile	thermophilicⓘ	no	96.4	no
[Ref.: #125438]	motile2+	flagellatedⓘ	no	78	no

Availability in culture collections External links

[Ref.: #67230]

Culture collection no.

DSM 110499, JCM 32923, CGMCC 1.13532, CGMCC 1.13535

[Ref.: #112582]

SI-ID 400396

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Occultella gossypii sp. nov., an alkali-resistant isolate from soil sampled in a cotton field.	Ge X, Huang R, Liu W	Int J Syst Evol Microbiol	10.1099/ijsem.0.005352	2022	*
Phylogeny	Identification of Haloactinobacterium kanbiaonis sp. nov. and Ruania zhangjianzhongii sp. nov., two novel species of the family Ruaniaceae isolated from faeces of bats (Hipposideros spp.).	Xu M, Dai Y, Huang Y, Yang J, Lai XH, Jin D, Lu S, Zhou J, Zhang S, Bai Y, Jiao Y, Qiao L, Jiang Y, Xu J	Int J Syst Evol Microbiol	10.1099/ijsem.0.004953	2021	*
Phylogeny	Haloactinobacterium glacieicola sp. nov., isolated from an ice core.	Wang F, Liu Y, Wang N, Xu B, Shen L, Gu Z, Liu K, Liu H, Zhou Y	Int J Syst Evol Microbiol	10.1099/ijsem.0.003653	2019	*
Phylogeny	Ruania suaedae sp. nov. and Ruania halotolerans sp. nov., two actinobacteria isolated from saline soil, and reclassification of Haloactinobacterium kanbiaonis as Occultella kanbiaonis comb. nov.	Xu L, Shang J, Tian J, Sun JQ, Shen B	Int J Syst Evol Microbiol	10.1099/ijsem.0.005443	2022	*

References

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#67230

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 110499

#67770 Japan Collection of Microorganism (JCM) ; Curators of the JCM;

#68213

Feng Wang, Yongqin Liu, Ninglian Wang, Baiqing Xu, Liang Shen, Zhengquan Gu, Keshao Liu, Hongcan Liu and Yuguang Zhou: Haloactinobacterium glacieicola sp. nov., isolated from an ice core. IJSEM 69: 3519 - 3523 2019 ( DOI 10.1099/ijsem.0.003653 )

#112582

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID400396.1 )

#125438

Julia Koblitz, Lorenz Christian Reimer, Rüdiger Pukall, Jörg Overmann: Predicting bacterial phenotypic traits through improved machine learning using high-quality, curated datasets. 2024 ( DOI 10.1101/2024.08.12.607695 )

* These data were automatically processed and therefore are not curated

Change proposal

You found an error in BacDive? Please tell us about it!

Note that changes will be reviewed and judged. If your changes are legitimate, changes will occur within the next BacDive update. Only proposed changes supported by the according reference will be reviewed. The BacDive team reserves the right to reject proposed changes.

Search for species Occultella glacieicola in external resources:
SILVA
BRENDA
PANGAEA
GBIF