Buchananella hordeovulneris (DSM 20732, ATCC 35275, CIP 103149)

Name: Buchananella hordeovulneris (DSM 20732, ATCC 35275, CIP 103149)
Creator: BacDive
License: http://creativecommons.org/licenses/by/4.0/

BacDive ID 160

Type strain

Culture col. no. DSM 20732 ATCC 35275 CIP 103149 CCUG 32937 CIP 103127 2 more

NCBI tax ID(s) 52770

Special Collections DSMZ CCUG CIP Literature strains

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Buchananella hordeovulneris DSM 20732 is a microaerophile, mesophilic, rod-shaped animal pathogen that was isolated from canine ascites fluid.

rod-shaped microaerophile mesophilic animal pathogen genome sequence 16S sequence

Name and taxonomic classification

SI-ID 92969

ATCC 35275,CCUG 32937,DSM 20732,CIP 103149,CCM 3913

@ref 20215

Last LPSN update 2025-12-16 09:46:47

Domain Bacillati

Phylum Actinomycetota

Class Actinomycetes

Order Actinomycetales

Family Actinomycetaceae

Genus Buchananella

Species Buchananella hordeovulneris

Full scientific name Buchananella hordeovulneris (Buchanan et al. 1984) Nouioui et al. 2018

Synonyms (1)

Actinomyces hordeovulneris

BacDive ID	Other strains from **Buchananella hordeovulneris** (4)	Type strain
147909	B. hordeovulneris CCUG 34476
147911	B. hordeovulneris CCUG 34478
148027	B. hordeovulneris CCUG 34706
148322	B. hordeovulneris CCUG 35223, CCM 3914

Morphology

Cell morphology

@ref	Cell shape
23107	rod-shaped
119966	rod-shaped
125438

Culture and growth conditions

Culture medium

@ref	Name	Medium link	Composition
9042	PYG MEDIUM (MODIFIED) (DSMZ Medium 104)	Medium recipe at MediaDive	Name: PYG MEDIUM (modified) (DSMZ Medium 104) Composition: Yeast extract 10.0 g/l Peptone 5.0 g/l Trypticase peptone 5.0 g/l Beef extract 5.0 g/l Glucose 5.0 g/l L-Cysteine HCl x H2O 0.5 g/l NaHCO3 0.4 g/l NaCl 0.08 g/l K2HPO4 0.04 g/l KH2PO4 0.04 g/l MgSO4 x 7 H2O 0.02 g/l CaCl2 x 2 H2O 0.01 g/l Hemin 0.005 g/l Ethanol 0.0038 g/l Resazurin 0.001 g/l Tween 80 Vitamin K1 NaOH Distilled water
23107	blood agar and 5% bovine blood agar and Trypticase soy or brain heart infusion agar
40541	MEDIUM 17 - for Actinomyces hordeovulneris		Distilled water make up to (1000.000 ml);Magnesium sulphate heptahydrate (0.200 g);Calcium chloride dihydrate (0.020 g);Agar (15.000 g);Glucose (5.000 g);Yeast extract (5.000 g);Ammonium sulphate (1.000 g);Casitone (4.000 g);Starch maize (1.000 g);Heart i
119966	CIP Medium 17	Medium recipe at CIP

Culture temp

@ref	Growth	Type	Temperature (°C)	Range
9042	positive	growth	37	mesophilic
40541	positive	growth	37	mesophilic

Physiology and metabolism

Oxygen tolerance

@ref	Oxygen tolerance
9042	microaerophile
23107	anaerobe
119966	facultative anaerobe
125439	facultative anaerobe

Metabolite utilization

@ref	Chebi-ID	Metabolite	Utilization activity	Kind of utilization tested
23107	22599 ChEBI	arabinose	-	builds acid from
68380	29016 ChEBI	arginine	-	hydrolysis	from API rID32A
23107	17057 ChEBI	cellobiose	+	builds acid from
68380	16024 ChEBI	D-mannose	-	fermentation	from API rID32A
23107	17234 ChEBI	glucose	+	builds acid from
23107	17754 ChEBI	glycerol	-	builds acid from
68380	29985 ChEBI	L-glutamate	-	degradation	from API rID32A
23107	17716 ChEBI	lactose	+	builds acid from
23107	17306 ChEBI	maltose	+	builds acid from
23107	29864 ChEBI	mannitol	-	builds acid from
23107	17268 ChEBI	myo-inositol	-	builds acid from
68380	17632 ChEBI	nitrate	-	reduction	from API rID32A
119966	17632 ChEBI	nitrate	-	reduction
119966	16301 ChEBI	nitrite	-	reduction
68380	16634 ChEBI	raffinose	-	fermentation	from API rID32A
23107	26546 ChEBI	rhamnose	-	builds acid from
23107	33942 ChEBI	ribose	-	builds acid from
23107	27082 ChEBI	trehalose	+	builds acid from
68380	27897 ChEBI	tryptophan	-	energy source	from API rID32A
68380	16199 ChEBI	urea	-	hydrolysis	from API rID32A
23107	18222 ChEBI	xylose	+	builds acid from

Antibiotic resistance

@ref	ChEBI	Metabolite	Sensitivity conc.
23107	3542	cephalothin	0.25 µg/mL
23107	17698	chloramphenicol	1 µg/mL
23107	18208	penicillin g	0.25 µg/mL
23107	45924	trimethoprim	1 µg/mL

Metabolite production

@ref	Chebi-ID	Metabolite	Production
68380	35581 ChEBI	indole		from API rID32A
119966	35581 ChEBI	indole

Metabolite tests

@ref	Chebi-ID	Metabolite	Indole test
68380	35581 ChEBI	indole	-	from API rID32A

Enzymes

@ref	Value	Activity	Ec
68382	acid phosphatase	-	3.1.3.2	from API zym
68380	alanine arylamidase	+	3.4.11.2	from API rID32A
68382	alkaline phosphatase	-	3.1.3.1	from API zym
68380	alkaline phosphatase	-	3.1.3.1	from API rID32A
68380	alpha-arabinosidase	-	3.2.1.55	from API rID32A
68382	alpha-chymotrypsin	-	3.4.21.1	from API zym
68382	alpha-fucosidase	-	3.2.1.51	from API zym
68380	alpha-fucosidase	-	3.2.1.51	from API rID32A
68382	alpha-galactosidase	+	3.2.1.22	from API zym
68380	alpha-galactosidase	+	3.2.1.22	from API rID32A
68382	alpha-glucosidase	-	3.2.1.20	from API zym
68382	alpha-mannosidase	-	3.2.1.24	from API zym
68380	arginine dihydrolase	-	3.5.3.6	from API rID32A
68382	beta-galactosidase	+	3.2.1.23	from API zym
68380	beta-galactosidase	+	3.2.1.23	from API rID32A
68380	beta-Galactosidase 6-phosphate	-		from API rID32A
68382	beta-glucosidase	-	3.2.1.21	from API zym
68380	beta-glucosidase	+	3.2.1.21	from API rID32A
68382	beta-glucuronidase	-	3.2.1.31	from API zym
68380	beta-glucuronidase	-	3.2.1.31	from API rID32A
23107	catalase	+/-	1.11.1.6
119966	catalase	-	1.11.1.6
68382	cystine arylamidase	-	3.4.11.3	from API zym
68382	esterase (C 4)	+		from API zym
68382	esterase lipase (C 8)	+		from API zym
68380	glutamate decarboxylase	-	4.1.1.15	from API rID32A
68380	glutamyl-glutamate arylamidase	-		from API rID32A
68380	glycin arylamidase	+		from API rID32A
68380	histidine arylamidase	+		from API rID32A
68380	L-arginine arylamidase	+		from API rID32A
68382	leucine arylamidase	+	3.4.11.1	from API zym
68380	leucine arylamidase	+	3.4.11.1	from API rID32A
68380	leucyl glycin arylamidase	+	3.4.11.1	from API rID32A
68382	lipase (C 14)	-		from API zym
68382	N-acetyl-beta-glucosaminidase	+	3.2.1.52	from API zym
68380	N-acetyl-beta-glucosaminidase	+	3.2.1.52	from API rID32A
68382	naphthol-AS-BI-phosphohydrolase	-		from API zym
119966	oxidase	-
68380	phenylalanine arylamidase	+		from API rID32A
68380	proline-arylamidase	+	3.4.11.5	from API rID32A
68380	serine arylamidase	+		from API rID32A
68382	trypsin	-	3.4.21.4	from API zym
68380	tryptophan deaminase	-	4.1.99.1	from API rID32A
68380	tyrosine arylamidase	+		from API rID32A
119966	urease	-	3.5.1.5
68380	urease	-	3.5.1.5	from API rID32A
68382	valine arylamidase	+		from API zym

Fatty acid profile

Metadata FA analysis

@ref

51269

Fatty acid	Percentage	ECL
C16:0	33.8	16
C15:0 ANTEISO	18.2	14.711
C14:0	15.6	14
C18:1 ω9c	8.2	17.769
C18:2 ω6,9c/C18:0 ANTE	8	17.724
C18:0	5	18
C15:0 ISO	3.1	14.621
C16:0 iso	2.8	15.626
C17:0 anteiso	2.8	16.722
C14:0 ISO	1.4	13.618
C13:0 ANTEISO	1.3	12.701

API zym

@ref	Control	Alkaline phosphatase	Esterase (C 4)	2-naphtyl caprylateEsterase Lipase (C 8)	Lipase (C 14)	L-leucyl-2-naphthylamideLeucine arylamidase	L-valyl-2-naphthylamideValine arylamidase	L-cystyl-2-naphthylamideCystine arylamidase	Trypsin	alpha- Chymotrypsin	Acid phosphatase	Naphthol-AS-BI-phosphateNaphthol-AS-BI-phosphohydrolase	alpha- Galactosidase	beta- Galactosidase	beta- Glucuronidase	alpha- Glucosidase	beta- Glucosidase	N-acetyl-beta- glucosaminidase	alpha- Mannosidase	alpha- Fucosidase
119966	-	-	+	+	-	+	+	-	-	-	-	-	+	+	-	-	-	+	-	-

API rID32A

@ref	URE	ADH (Arg)	alpha GAL	beta GAL	beta-Galactosidase 6-phosphatebeta GP	alpha GLU	beta GLU	alpha ARA	beta GUR	beta-N-Acetyl-beta-glucosaminidasebeta NAG	MNE	RAF	GDC	alpha FUC	Reduction of nitrateNIT	IND	PAL	L-arginine arylamidaseArgA	ProA	LGA	Phenylalanine arylamidasePheA	Leucine arylamidaseLeuA	PyrA	Tyrosine arylamidaseTyrA	Alanine arylamidaseAlaA	Glycin arylamidaseGlyA	Histidine arylamidaseHisA	Glutamyl-glutamate arylamidaseGGA	Serine arylamidaseSerA
9042	-	-	+	+	-	+/-	+	-	-	+	-	-	-	-	-	-	-	+	+	+	+	+	+/-	+	+	+	+	-	+

Isolation, sampling and environmental information

Isolation source categories

Cat1	Cat2	Cat3
#Host	#Mammals	#Canidae (Dog)
#Host Body-Site	#Gastrointestinal tract	#Stomach
#Host Body Product	#Fluids	-

Isolation

@ref	Sample type	Geographic location	Country	Country ISO 3 Code	Continent
9042	canine ascites fluid	California	USA	USA	North America
51269	Canine ascites fluid
119966	Animal, Canine, ascites fluid

Taxonmaps

69479

Global distribution of 16S sequence X82448 (>99% sequence identity) for Buchananella hordeovulneris subclade from Microbeatlas

Aquatic Samples	122
Soil Samples	234
Animal Samples	3650
Plant Samples	105
Total Samples	4111

Interaction and safety

Risk assessment

@ref	Pathogenicity animal	Biosafety level	Biosafety level comment
9042		2	Risk group (German classification) According to TRBA 466
119966		2	Risk group (French classification) According to TRBA 466

Sequence information

Genome sequences

@ref	Description	Assembly level	INSDC accession	BV-BRC accession	IMG accession	NCBI tax ID	Score
66792	ASM190723v1 assembly for Buchananella hordeovulneris DSM 20732	scaffold	GCA_001907235	52770.4	2857276557	52770	72.24

Genome browser: GCA_001907235

16S sequences

@ref	Description	Accession	Length	Database	NCBI tax ID
20218	A.hordeovulneris 16S rRNA gene	X82448	1426		52770

GC content

@ref	GC-content (mol%)	Method
9042	67.0	Buoyant density centrifugation (BD)

Genome-based predictions

Predictions

Predictions from Deep Learning for Genome Sequence Data. R package version 0.3.1 based on: Buchananella hordeovulneris DSM 20732
NCBI: GCA_001907235

@ref	Trait	Model	Prediction	Confidence in %	In training data
125439	spore_formation	BacteriaNetⓘ	no	64.00	no
125439	motility	BacteriaNetⓘ	no	89.10	no
125439	gram_stain	BacteriaNetⓘ	positive	85.50	no
125439	oxygen_tolerance	BacteriaNetⓘ	facultative anaerobe	90.30	no

Predictions from bacterial phenotypic traits through improved machine learning using high-quality, curated datasets based on: Buchananella hordeovulneris DSM 20732
NCBI: GCA_001907235.1

@ref	Trait	Model	Prediction	Confidence in %	In training data
125438	gram-positive	gram-positiveⓘ	yes	87.08	no
125438	anaerobic	anaerobicⓘ	no	69.48	no
125438	aerobic	aerobicⓘ	no	82.58	yes
125438	spore-forming	spore-formingⓘ	no	82.52	no
125438	thermophilic	thermophileⓘ	no	90.81	no
125438	flagellated	motile2+ⓘ	no	91.38	no

Literature

Topic	Title	Authors	Journal	DOI	Year
Genetics	Genome-Based Taxonomic Classification of the Phylum Actinobacteria.	Nouioui I, Carro L, Garcia-Lopez M, Meier-Kolthoff JP, Woyke T, Kyrpides NC, Pukall R, Klenk HP, Goodfellow M, Goker M.	Front Microbiol	10.3389/fmicb.2018.02007	2018
	Isolation and characterization of Buchananella hordeovulneris from feline pyothorax.	Samoel GVA, Herbichi AP, Nunes GT, Machado CS, Fernandes FD, Vogel FSF, Cargnelutti JF.	Braz J Microbiol	10.1007/s42770-022-00845-8	2022
	Influence of Gallic Acid-Containing Mouth Spray on Dental Health and Oral Microbiota of Healthy Dogs: A Pilot Study.	Thongma N, Sivamaruthi BS, Bharathi M, Tansrisook C, Peerajan S, Tanongpitchayes K, Chawnan N, Rashmi S, Thongkorn K, Chaiyasut C.	Vet Sci	10.3390/vetsci10070424	2023
	A Randomized Controlled Trial to Evaluate the Impact of a Novel Probiotic and Nutraceutical Supplement on Pruritic Dermatitis and the Gut Microbiota in Privately Owned Dogs.	Tate DE, Tanprasertsuk J, Jones RB, Maughan H, Chakrabarti A, Khafipour E, Norton SA, Shmalberg J, Honaker RW.	Animals (Basel)	10.3390/ani14030453	2024
	An Update on Novel Taxa and Revised Taxonomic Status of Bacteria Isolated from Domestic Animals Described in 2018 to 2021.	Munson E, Lawhon SD, Burbick CR, Zapp A, Villaflor M, Thelen E.	J Clin Microbiol	10.1128/jcm.00281-22	2023
Phylogeny	Actinomyces hordeovulneris, a canine pathogen that produces L-phase variants spontaneously with coincident calcium deposition.	Buchanan AM, Scott JL	Am J Vet Res		1984
Phylogeny	Buchananella felis sp. nov. isolated from feline pyothorax.	Feyer S, Fuss S, Gross N, Zumwald S, Brodard I, Kittl S.	Int J Syst Evol Microbiol	10.1099/ijsem.0.006723	2025

References

#9042	Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 20732
#20215	Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )
#20218	Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.: StrainInfo introduces electronic passports for microorganisms.. Syst Appl Microbiol. 37: 42 - 50 2014 ( DOI 10.1016/j.syapm.2013.11.002 , PubMed 24321274 )
#23107	Audria M. Buchanan, Jamon L. Scott, Mary Ann Gerencser, Blaine L. Beaman, Spencer Jang, Ernst L. Biberstein: Actinomyces hordeovulneris sp. nov., an Agent of Canine Actinomycosis. IJSEM 34: 439 - 443 1984 ( DOI 10.1099/00207713-34-4-439 )
#40541	; Curators of the CIP;
#51269	Culture Collection University of Gothenburg (CCUG) ; Curators of the CCUG; CCUG 32937
#66792	Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .
#68380	Automatically annotated from API rID32A .
#68382	Automatically annotated from API zym .
#69479	João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .
#119966	Collection of Institut Pasteur ; Curators of the CIP; CIP 103149
#125438	Julia Koblitz, Lorenz Christian Reimer, Rüdiger Pukall, Jörg Overmann: Predicting bacterial phenotypic traits through improved machine learning using high-quality, curated datasets. 2024 ( DOI 10.1101/2024.08.12.607695 )
#125439	Philipp Münch, René Mreches, Martin Binder, Hüseyin Anil Gündüz, Xiao-Yin To, Alice McHardy: deepG: Deep Learning for Genome Sequence Data. R package version 0.3.1 .
#126262	A. Lissin, I. Schober, J. F. Witte, H. Lüken, A. Podstawka, J. Koblitz, B. Bunk, P. Dawyndt, P. Vandamme, P. de Vos, J. Overmann, L. C. Reimer: StrainInfo—the central database for linked microbial strain identifiers. ( DOI 10.1093/database/baaf059 )

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Buchananella hordeovulneris (DSM 20732, ATCC 35275, CIP 103149)

Name and taxonomic classification