Name: Buchananella hordeovulneris DSM 20732
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 160

Type strain:

Species: Buchananella hordeovulneris

Culture col. no.: DSM 20732, ATCC 35275, CIP 103149, CCUG 32937, CIP 103127, UCD 81-332-9, UCD 332-981

Strain history: CIP <- 1988, ATCC <- A.M. Buchanan: strain UCD 332-981

NCBI tax ID(s): 52770 (species)

SI-ID 92969

ATCC 35275, CCUG 32937, DSM 20732, CIP 103149, CCM 3913

Other strains from Buchananella hordeovulneris

B. hordeovulneris CCUG 34476
B. hordeovulneris CCUG 34478
B. hordeovulneris CCUG 34706
B. hordeovulneris CCUG 35223, CCM 3914

Section

Buchananella hordeovulneris DSM 20732 is a microaerophile, mesophilic, rod-shaped animal pathogen that was isolated from canine ascites fluid.

rod-shaped
animal pathogen
mesophilic
microaerophile
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Actinomycetota*
	Class	*Actinomycetes*
	Order	*Actinomycetales*
	Family	*Actinomycetaceae*
	Genus	*Buchananella*
	Species	**Buchananella hordeovulneris**
	Full Scientific Name (LPSN)	Buchananella hordeovulneris (Buchanan et al. 1984) Nouioui et al. 2018

Synonym

Actinomyces hordeovulneris

Information on morphological and physiological properties Morphology

[Ref.: #119966]	Gram stain	positive
[Ref.: #69480]	Gram stain	positive Confidence in %100

[Ref.: #119966]	Cell shape	rod-shaped
[Ref.: #23107]	Cell shape	rod-shaped

[Ref.: #119966]	Motility	no

Information on culture and growth conditions Culture and growth conditions

[Ref.: #9042]

Culture medium

PYG MEDIUM (MODIFIED) (DSMZ Medium 104)

[Ref.: #9042]

Culture medium growth

[Ref.: #9042]

Culture medium link

Medium recipe at MediaDive

[Ref.: #9042]

Culture medium composition

expand / minimize

[Ref.: #23107]

Culture medium

blood agar and 5% bovine blood agar and Trypticase soy or brain
heart infusion agar

[Ref.: #23107]

Culture medium growth

[Ref.: #40541]

Culture medium

MEDIUM 17 - for Actinomyces hordeovulneris

[Ref.: #40541]

Culture medium growth

[Ref.: #40541]

Culture medium composition

expand / minimize

[Ref.: #119966]

Culture medium

CIP Medium 17

[Ref.: #119966]

Culture medium growth

[Ref.: #119966]

Culture medium link

Medium recipe provided by DSMZ

	Temperatures	Kind of temperature		Temperature
[Ref.: #9042]			growth	37 °C
[Ref.: #40541]			growth	37 °C

[Ref.: #9042]	Temperature range	mesophilic
[Ref.: #40541]	Temperature range	mesophilic

Information on physiology and metabolism Physiology and metabolism

[Ref.: #9042]	Oxygen tolerance	microaerophile
[Ref.: #23107]	Oxygen tolerance	anaerobe
[Ref.: #119966]	Oxygen tolerance	facultative anaerobe
[Ref.: #69480]	Oxygen tolerance	anaerobe Confidence in %91.301

[Ref.: #69480]

Ability of spore formation

no Confidence in %99.965

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #23107]	arabinose ChEBI	-	builds acid from
[Ref.: #68380]	arginine ChEBI	-	hydrolysis	* from API rID32A
[Ref.: #23107]	cellobiose ChEBI	+	builds acid from
[Ref.: #68380]	D-mannose ChEBI	-	fermentation	* from API rID32A
[Ref.: #23107]	glucose ChEBI	+	builds acid from
[Ref.: #23107]	glycerol ChEBI	-	builds acid from
[Ref.: #68380]	L-glutamate ChEBI	-	degradation	* from API rID32A
[Ref.: #23107]	lactose ChEBI	+	builds acid from
[Ref.: #23107]	maltose ChEBI	+	builds acid from
[Ref.: #23107]	mannitol ChEBI	-	builds acid from
[Ref.: #23107]	myo-inositol ChEBI	-	builds acid from
[Ref.: #119966]	nitrate ChEBI	-	reduction
[Ref.: #68380]	nitrate ChEBI	-	reduction	* from API rID32A
[Ref.: #119966]	nitrite ChEBI	-	reduction
[Ref.: #68380]	raffinose ChEBI	-	fermentation	* from API rID32A
[Ref.: #23107]	rhamnose ChEBI	-	builds acid from
[Ref.: #23107]	ribose ChEBI	-	builds acid from
[Ref.: #23107]	trehalose ChEBI	+	builds acid from
[Ref.: #68380]	tryptophan ChEBI	-	energy source	* from API rID32A
[Ref.: #68380]	urea ChEBI	-	hydrolysis	* from API rID32A
[Ref.: #23107]	xylose ChEBI	+	builds acid from
	Only first 10 entries are displayed. Click here to see all.

	Metabolite	Antibiotic sensitivity	Concentration
[Ref.: #23107]	cephalothin ChEBI	yes	0.25	µg/mL
[Ref.: #23107]	chloramphenicol ChEBI	yes	1	µg/mL
[Ref.: #23107]	penicillin g ChEBI	yes	0.25	µg/mL
[Ref.: #23107]	trimethoprim ChEBI	yes	1	µg/mL

	Metabolite production	Metabolite	Production
[Ref.: #119966]		indole ChEBI	no
[Ref.: #68380]		indole ChEBI	no	* from API rID32A

	Physiological tests	Metabolite	Indole test
[Ref.: #68380]		indole ChEBI	-	* from API rID32A

	Enzyme	Enzyme activity	EC number
[Ref.: #68382]	acid phosphatase	-	3.1.3.2	* from API zym
[Ref.: #68380]	alanine arylamidase	+	3.4.11.2	* from API rID32A
[Ref.: #68382]	alkaline phosphatase	-	3.1.3.1	* from API zym, API rID32A
[Ref.: #68380]	alpha-arabinosidase	-	3.2.1.55	* from API rID32A
[Ref.: #68382]	alpha-chymotrypsin	-	3.4.21.1	* from API zym
[Ref.: #68382]	alpha-fucosidase	-	3.2.1.51	* from API zym, API rID32A
[Ref.: #68382]	alpha-galactosidase	+	3.2.1.22	* from API zym, API rID32A
[Ref.: #68382]	alpha-glucosidase	-	3.2.1.20	* from API zym
[Ref.: #68382]	alpha-mannosidase	-	3.2.1.24	* from API zym
[Ref.: #68380]	arginine dihydrolase	-	3.5.3.6	* from API rID32A
[Ref.: #68382]	beta-galactosidase	+	3.2.1.23	* from API zym, API rID32A
[Ref.: #68380]	beta-Galactosidase 6-phosphate	-		* from API rID32A
[Ref.: #68380]	beta-glucosidase	+	3.2.1.21	* from API rID32A
[Ref.: #68382]	beta-glucosidase	-	3.2.1.21	* from API zym
[Ref.: #68382]	beta-glucuronidase	-	3.2.1.31	* from API zym, API rID32A
[Ref.: #23107]	catalase	+/-	1.11.1.6
[Ref.: #119966]	catalase	-	1.11.1.6
[Ref.: #68382]	cystine arylamidase	-	3.4.11.3	* from API zym
[Ref.: #68382]	esterase (C 4)	+		* from API zym
[Ref.: #68382]	esterase lipase (C 8)	+		* from API zym
[Ref.: #68380]	glutamate decarboxylase	-	4.1.1.15	* from API rID32A
[Ref.: #68380]	glutamyl-glutamate arylamidase	-		* from API rID32A
[Ref.: #68380]	glycin arylamidase	+		* from API rID32A
[Ref.: #68380]	histidine arylamidase	+		* from API rID32A
[Ref.: #68380]	L-arginine arylamidase	+		* from API rID32A
[Ref.: #68382]	leucine arylamidase	+	3.4.11.1	* from API zym, API rID32A
[Ref.: #68380]	leucyl glycin arylamidase	+	3.4.11.1	* from API rID32A
[Ref.: #68382]	lipase (C 14)	-		* from API zym
[Ref.: #68382]	N-acetyl-beta-glucosaminidase	+	3.2.1.52	* from API zym, API rID32A
[Ref.: #68382]	naphthol-AS-BI-phosphohydrolase	-		* from API zym
[Ref.: #119966]	oxidase	-
[Ref.: #68380]	phenylalanine arylamidase	+		* from API rID32A
[Ref.: #68380]	proline-arylamidase	+	3.4.11.5	* from API rID32A
[Ref.: #68380]	serine arylamidase	+		* from API rID32A
[Ref.: #68382]	trypsin	-	3.4.21.4	* from API zym
[Ref.: #68380]	tryptophan deaminase	-	4.1.99.1	* from API rID32A
[Ref.: #68380]	tyrosine arylamidase	+		* from API rID32A
[Ref.: #119966]	urease	-	3.5.1.5
[Ref.: #68380]	urease	-	3.5.1.5	* from API rID32A
[Ref.: #68382]	valine arylamidase	+		* from API zym
	Only first 10 entries are displayed. Click here to see all.

Fatty acid profile

Metadata FA analysis

Reference

[Ref.: #51269]

Fatty acid	Percentage	ECL
C16:0	33.8	16
C15:0 ANTEISO	18.2	14.711
C14:0	15.6	14
C18:1 ω9c	8.2	17.769
C18:2 ω6,9c/C18:0 ANTE	8	17.724
C18:0	5	18
C15:0 ISO	3.1	14.621
C16:0 iso	2.8	15.626
C17:0 anteiso	2.8	16.722
C14:0 ISO	1.4	13.618
C13:0 ANTEISO	1.3	12.701

API® rID32A:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Urease/urea hydrolysisURE	Arginine dihydrolaseADH (Arg)	alpha-Galactosidasealpha GAL	beta-Galactosidasebeta GAL	beta-Galactosidase 6-phosphatebeta GP	alpha-Glucosidasealpha GLU	beta-Glucosidasebeta GLU	alpha-Arabinosidasealpha ARA	beta-Glucuronidasebeta GUR	beta-N-Acetyl-beta-glucosaminidasebeta NAG	Fermentation of D-mannoseMNE	Fermentation of raffinoseRAF	Glutamate decarboxylaseGDC	alpha-Fucosidasealpha FUC	Reduction of nitrateNIT	Indole productionIND	Alkaline phosphatasePAL	L-arginine arylamidaseArgA	Proline arylamidaseProA	Leucyl glycin arylamidaseLGA	Phenylalanine arylamidasePheA	Leucine arylamidaseLeuA	Pyrrolidonyl arylamidasePyrA	Tyrosine arylamidaseTyrA	Alanine arylamidaseAlaA	Glycin arylamidaseGlyA	Histidine arylamidaseHisA	Glutamyl-glutamate arylamidaseGGA	Serine arylamidaseSerA
[Ref.: #9042]	12735	-	-	+	+	-	+/-	+	-	-	+	-	-	-	-	-	-	-	+	+	+	+	+	+/-	+	+	+	+	-	+

API® zym:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Control	2-naphthyl phosphateAlkaline phosphatase	2-naphthyl butyrateEsterase (C 4)	2-naphtyl caprylateEsterase Lipase (C 8)	2-naphthyl myristateLipase (C 14)	L-leucyl-2-naphthylamideLeucine arylamidase	L-valyl-2-naphthylamideValine arylamidase	L-cystyl-2-naphthylamideCystine arylamidase	N-benzoyl-DL-arginine-2-naphthylamideTrypsin	N-glutaryl-phenylalanine-2-naphthylamidealpha- Chymotrypsin	2-naphthyl phosphateAcid phosphatase	Naphthol-AS-BI-phosphateNaphthol-AS-BI-phosphohydrolase	6-Br-2-naphthyl-alphaD-galactpyranosidealpha- Galactosidase	2-naphthyl-betaD-galactpyranosidebeta- Galactosidase	Naphthol-AS-BI-betaD-glucuronidebeta- Glucuronidase	2-naphthyl-alphaD-glucopyranosidealpha- Glucosidase	6-Br-2-naphthyl-betaD-glucopyranosidebeta- Glucosidase	1-naphthyl-N-acetyl-betaD-glucosaminideN-acetyl-beta- glucosaminidase	6-Br-2-naphthyl-alphaD-mannopyranosidealpha- Mannosidase	2-naphthyl-alphaL-fucopyranosidealpha- Fucosidase
[Ref.: #119966]	7392	-	-	+	+	-	+	+	-	-	-	-	-	+	+	-	-	-	+	-	-

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #9042]	Sample type/isolated from	canine ascites fluid
[Ref.: #9042]	Geographic location (country and/or sea, region)	California
[Ref.: #9042]	Country	USA
[Ref.: #9042]	Country ISO 3 Code	USA
[Ref.: #9042]	Continent	North America

[Ref.: #51269]	Sample type/isolated from	Canine ascites fluid

[Ref.: #119966]	Sample type/isolated from	Animal, Canine, ascites fluid
* marker position based on {}

Isolation sources categories

#Host	#Mammals	#Canidae (Dog)
#Host Body-Site	#Gastrointestinal tract	#Stomach
#Host Body Product	#Fluids	-

What are isolation sources categories?

[Ref.: #69479]

Global distribution of 16S sequence X82448 (>99% sequence identity) for Buchananella hordeovulneris subclade from Microbeatlas

Aquatic Samples	122
Soil Samples	234
Animal Samples	3650
Plant Samples	105
Total Samples	4111

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #9042]	Pathogenicity (animal)	yes
[Ref.: #9042]	Biosafety level	2	Risk group (German classification) According to TRBA 466
[Ref.: #119966]	Biosafety level	2	Risk group (French classification)

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #20218]	A.hordeovulneris 16S rRNA gene	X82448	1426	ENA	52770 tax ID	*

Genome sequence information:

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #66792]	Buchananella hordeovulneris DSM 20732	GCA_001907235	scaffold	GenBank	52770 tax ID	*
[Ref.: #66792]	Actinomyces hordeovulneris strain DSM 20732	52770.4	wgs	PATRIC	52770 tax ID	*
[Ref.: #66792]	Actinomyces hordeovulneris DSM 20732	2857276557	draft	JGI IMG/M	52770 tax ID	*

[Ref.: #9042]

GC-content

67.0 mol%

Buoyant density centrifugation (BD)

Data predicted using genome information as a basis Genome-based predictions

	Trait	Prediction	Confidence in %	In training data
	Predictions from GenomeNet Sporulation v. 1 based on: Buchananella hordeovulneris DSM 20732 NCBI: GCA_001907235.1
[Ref.: #69481]	spore-forming	yes	66	no

	Trait	Prediction	Confidence in %	In training data
	Predictions from Diaspora project based on: Buchananella hordeovulneris DSM 20732 NCBI: GCA_001907235.1
[Ref.: #69480]	flagellated	no	97.415	no
[Ref.: #69480]	gram-positive	yes	94.388	no
[Ref.: #69480]	anaerobic	no	73.793	no
[Ref.: #69480]	aerobic	no	94.723	no
[Ref.: #69480]	halophile	no	85.234	no
[Ref.: #69480]	spore-forming	no	91.435	no
[Ref.: #69480]	glucose-util	yes	82.834	no
[Ref.: #69480]	thermophile	no	93.59	no
[Ref.: #69480]	motile	no	91.312	no
[Ref.: #69480]	glucose-ferment	yes	66.321	no

Availability in culture collections External links

[Ref.: #9042]

Culture collection no.

DSM 20732, ATCC 35275, CIP 103149, CCUG 32937, CIP 103127, UCD 81-332-9, UCD 332-981

[Ref.: #69842]

SI-ID 92969

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Actinomyces hordeovulneris, a canine pathogen that produces L-phase variants spontaneously with coincident calcium deposition.	Buchanan AM, Scott JL	Am J Vet Res		1984	*

References

#9042

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 20732

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#20218

Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.: StrainInfo introduces electronic passports for microorganisms.. Syst Appl Microbiol. 37: 42 - 50 2014 ( DOI 10.1016/j.syapm.2013.11.002 , PubMed 24321274 )

#23107

Audria M. Buchanan, Jamon L. Scott, Mary Ann Gerencser, Blaine L. Beaman, Spencer Jang, Ernst L. Biberstein: Actinomyces hordeovulneris sp. nov., an Agent of Canine Actinomycosis. IJSEM 34: 439 - 443 1984 ( DOI 10.1099/00207713-34-4-439 )

#40541 ; Curators of the CIP;
#51269 Culture Collection University of Gothenburg (CCUG) ; Curators of the CCUG; CCUG 32937

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#68380

Automatically annotated from API rID32A .

#68382

Automatically annotated from API zym .

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

#69480

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#69481

Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch: Predictions based on the model GenomeNet Sporulation v. 1 . ( DOI 10.21203/rs.3.rs-2527258/v1 )

#69842

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID92969.1 )

#119966 Collection of Institut Pasteur ; Curators of the CIP; CIP 103149
* These data were automatically processed and therefore are not curated

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