Name: Acetobacter orientalis 21F-2
Creator: BacDive
License: https://creativecommons.org/licenses/by/4.0/

Strain identifier

BacDive ID: 16

Type strain:

Species: Acetobacter orientalis

Strain Designation: 21F-2

Culture col. no.: DSM 15550, IFO 16606, NBRC 16606, NRIC 0481, JCM 11195, CIP 107379

Strain history: CIP <- 2002, JCM <- 2001, NRIC <- P. Lisdiyanti: strain 21F-2

NCBI tax ID(s): 1307913 (strain), 146474 (species)

SI-ID 265202

IFO 16606, JCM 11195, CIP 107379, NBRC 16606, DSM 15550

Special Collections

Section

Acetobacter orientalis 21F-2 is an obligate aerobe, mesophilic, Gram-negative bacterium that was isolated from canna flower, Canna hybrida.

Gram-negative
rod-shaped
mesophilic
obligate aerobe
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	09-12-2024 (DD-MM-YYYY)
	Domain	*Bacteria*
	Phylum	*Pseudomonadota*
	Class	*Alphaproteobacteria*
	Order	*Rhodospirillales*
	Family	*Acetobacteraceae*
	Genus	*Acetobacter*
	Species	**Acetobacter orientalis**
	Full Scientific Name (LPSN)	Acetobacter orientalis Lisdiyanti et al. 2002

Information on morphological and physiological properties Morphology

[Ref.: #121435]	Gram stain	negative
[Ref.: #69480]	Gram stain	negative Confidence in %95.833

[Ref.: #121435]	Cell shape	rod-shaped

[Ref.: #121435]	Motility	no

Information on culture and growth conditions Culture and growth conditions

[Ref.: #5996]

Culture medium

ACETIC ACID BACTERIUM MEDIUM (DSMZ Medium 989)

[Ref.: #5996]

Culture medium growth

[Ref.: #5996]

Culture medium link

Medium recipe at MediaDive

[Ref.: #5996]

Culture medium composition

expand / minimize

[Ref.: #33782]

Culture medium

MEDIUM 72- for trypto casein soja agar

[Ref.: #33782]

Culture medium growth

[Ref.: #33782]

Culture medium composition

expand / minimize

[Ref.: #121435]

Culture medium

CIP Medium 72

[Ref.: #121435]

Culture medium growth

[Ref.: #121435]

Culture medium link

Medium recipe provided by DSMZ

	Kind of temperature		Temperature
[Ref.: #5996]		growth	30 °C
[Ref.: #33782]		growth	30 °C
[Ref.: #67770]		growth	30 °C
[Ref.: #121435]		growth	22-37 °C
[Ref.: #121435]	no	growth	10 °C
[Ref.: #121435]	no	growth	41 °C
[Ref.: #121435]	no	growth	45 °C

Information on physiology and metabolism Physiology and metabolism

[Ref.: #121435]

Oxygen tolerance

obligate aerobe

[Ref.: #69480]

Ability of spore formation

no Confidence in %92.965

	Salt	Tested relation		Salt conc.
[Ref.: #121435]	NaCl	no	growth	0	%
[Ref.: #121435]	NaCl	no	growth	2	%
[Ref.: #121435]	NaCl	no	growth	4	%
[Ref.: #121435]	NaCl	no	growth	6	%
[Ref.: #121435]	NaCl	no	growth	8	%
[Ref.: #121435]	NaCl	no	growth	10	%

[Ref.: #67770]

Observation

quinones: Q-9

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #121435]	hippurate ChEBI	+	hydrolysis
[Ref.: #121435]	nitrate ChEBI	-	reduction
[Ref.: #121435]	nitrite ChEBI	-	reduction

	Antibiotica	Metabolite	Antibiotic sensitivity	Antibiotic resistance
[Ref.: #121435]		0129 (2,4-Diamino-6,7-di-iso-propylpteridine phosphate)	no	yes

	Metabolite production	Metabolite	Production
[Ref.: #121435]		indole ChEBI	no

	Enzyme	Enzyme activity	EC number
[Ref.: #68382]	acid phosphatase	-	3.1.3.2	* from API zym
[Ref.: #68382]	alkaline phosphatase	-	3.1.3.1	* from API zym
[Ref.: #68382]	alpha-chymotrypsin	-	3.4.21.1	* from API zym
[Ref.: #68382]	alpha-fucosidase	-	3.2.1.51	* from API zym
[Ref.: #68382]	alpha-galactosidase	-	3.2.1.22	* from API zym
[Ref.: #68382]	alpha-glucosidase	-	3.2.1.20	* from API zym
[Ref.: #68382]	alpha-mannosidase	-	3.2.1.24	* from API zym
[Ref.: #121435]	beta-galactosidase	-	3.2.1.23
[Ref.: #68382]	beta-galactosidase	-	3.2.1.23	* from API zym
[Ref.: #68382]	beta-glucosidase	-	3.2.1.21	* from API zym
[Ref.: #68382]	beta-glucuronidase	-	3.2.1.31	* from API zym
[Ref.: #121435]	catalase	+	1.11.1.6
[Ref.: #68382]	cystine arylamidase	-	3.4.11.3	* from API zym
[Ref.: #68382]	esterase (C 4)	+		* from API zym
[Ref.: #68382]	esterase lipase (C 8)	-		* from API zym
[Ref.: #121435]	gamma-glutamyltransferase	-	2.3.2.2
[Ref.: #121435]	gelatinase	-
[Ref.: #68382]	leucine arylamidase	+	3.4.11.1	* from API zym
[Ref.: #68382]	lipase (C 14)	-		* from API zym
[Ref.: #68382]	N-acetyl-beta-glucosaminidase	-	3.2.1.52	* from API zym
[Ref.: #68382]	naphthol-AS-BI-phosphohydrolase	-		* from API zym
[Ref.: #121435]	oxidase	-
[Ref.: #68382]	trypsin	-	3.4.21.4	* from API zym
[Ref.: #121435]	urease	-	3.5.1.5
[Ref.: #68382]	valine arylamidase	-		* from API zym
	Only first 10 entries are displayed. Click here to see all.

API® Biotype100:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	ControlQ	D-glucose as carbon sourceGLU	D-fructose as carbon sourceFRU	D-galactose as carbon sourceGAL	D-trehalose as carbon sourceTRE	D-mannose as carbon sourceMNE	L-sorbose as carbon sourceSBE	D-melibiose as carbon sourceMEL	sucrose as carbon sourceSAC	D-raffinose as carbon sourceRAF	maltotriose as carbon sourceMTE	maltose as carbon sourceMAL	lactose as carbon sourceLAC	lactulose as carbon sourceLTE	1-O-methyl-beta-galactopyranoside as carbon sourceMbGa	1-O-methyl-alpha-galactopyranoside as carbon sourceMaGa	D-cellobiose as carbon sourceCEL	gentiobiose as carbon sourceGEN	1-O-methyl-beta-D-glucopyranoside as carbon sourceMbGu	esculin as carbon sourceESC	D-ribose as carbon sourceRIB	L-arabinose as carbon sourceARA	D-xylose as carbon sourceXYL	palatinose as carbon sourcePLE	L-rhamnose as carbon sourceRHA	L-fucose as carbon sourceFUC	D-melezitose as carbon sourceMLZ	D-arabitol as carbon sourceDARL	L-arabitol as carbon sourceLARL	xylitol as carbon sourceXLT	dulcitol as carbon sourceDUL	D-tagatose as carbon sourceTAG	glycerol as carbon sourceGLY	myo-inositol as carbon sourceINO	D-mannitol as carbon sourceMAN	maltitol as carbon sourceMTL	D-turanose as carbon sourceTUR	D-sorbitol as carbon sourceSOR	adonitol as carbon sourceADO	hydroxyquinoline-beta-glucuronide as carbon sourceHBG	D-lyxose as carbon sourceLYX	i-erythritol as carbon sourceERY	1-O-methyl-alpha-D-glucoside as carbon sourceMDG	3-O-methyl-D-glucose as carbon source3MDG	D-saccharate as carbon sourceSAT	mucate as carbon sourceMUC	L-tartrate as carbon sourceLTAT	D-tartrate as carbon sourceDTAT	meso-tartrate as carbon sourceMTAT	D-malate as carbon sourceDMLT	L-malate as carbon sourceLMLT	cis-aconitate as carbon sourceCATE	trans-aconitate as carbon sourceTATE	tricarballylate as carbon sourceTTE	citrate as carbon sourceCIT	D-glucuronate as carbon sourceGRT	D-galacturonate as carbon sourceGAT	2-ketogluconate as carbon source2KG	5-ketogluconate as carbon source5KG	tryptophan as carbon sourceTRY	N-acetyl-D-glucosamine as carbon sourceNAG	D-gluconate as carbon sourceGNT	phenylacetate as carbon sourcePAC	protocatechuate as carbon sourcePAT	4-hydroxybenzoate as carbon sourcepOBE	quinate as carbon sourceQAT	gentisate as carbon sourceGTE	3-hydroxybenzoate as carbon sourcemOBE	benzoate as carbon sourceBAT	3-phenylpropionate as carbon sourcePPAT	m-coumarate as carbon sourceCMT	trigonelline as carbon sourceTGE	betaine as carbon sourceBET	putrescine as carbon sourcePCE	4-aminobutyrate as carbon sourceABT	histamine as carbon sourceHIN	DL-lactate as carbon sourceLAT	caprate as carbon sourceCAP	caprylate as carbon sourceCYT	L-histidine as carbon sourceHIS	succinate as carbon sourceSUC	fumarate as carbon sourceFUM	glutarate as carbon sourceGRE	DL-glycerate as carbon sourceGYT	5-aminovalerate as carbon sourceAVT	ethanolamine as carbon sourceETN	tryptamine as carbon sourceTTN	D-glucosamine as carbon sourceGLN	itaconate as carbon sourceITA	3-hydroxybutyrate as carbon source3OBU	L-aspartate as carbon sourceAPT	L-glutamate as carbon sourceGTT	L-proline as carbon sourcePRO	D-alanine as carbon sourceDALA	L-alanine as carbon sourceLALA	L-serine as carbon sourceSER	malonate as carbon sourceMNT	propionate as carbon sourcePROP	L-tyrosine as carbon sourceTYR	2-ketoglutarate as carbon source2KT
[Ref.: #121435]	2186	not determinedn.d.	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	+	-	-	+	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	+	-	-	-	-	-	-	-	-	-	-	-	-

API® zym:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Control	2-naphthyl phosphateAlkaline phosphatase	2-naphthyl butyrateEsterase (C 4)	2-naphtyl caprylateEsterase Lipase (C 8)	2-naphthyl myristateLipase (C 14)	L-leucyl-2-naphthylamideLeucine arylamidase	L-valyl-2-naphthylamideValine arylamidase	L-cystyl-2-naphthylamideCystine arylamidase	N-benzoyl-DL-arginine-2-naphthylamideTrypsin	N-glutaryl-phenylalanine-2-naphthylamidealpha- Chymotrypsin	2-naphthyl phosphateAcid phosphatase	Naphthol-AS-BI-phosphateNaphthol-AS-BI-phosphohydrolase	6-Br-2-naphthyl-alphaD-galactpyranosidealpha- Galactosidase	2-naphthyl-betaD-galactpyranosidebeta- Galactosidase	Naphthol-AS-BI-betaD-glucuronidebeta- Glucuronidase	2-naphthyl-alphaD-glucopyranosidealpha- Glucosidase	6-Br-2-naphthyl-betaD-glucopyranosidebeta- Glucosidase	1-naphthyl-N-acetyl-betaD-glucosaminideN-acetyl-beta- glucosaminidase	6-Br-2-naphthyl-alphaD-mannopyranosidealpha- Mannosidase	2-naphthyl-alphaL-fucopyranosidealpha- Fucosidase
[Ref.: #121435]	10311	-	-	+	-	-	+	-	-	-	-	-	-	-	-	-	-	-	-	-	-

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #5996]	Sample type/isolated from	canna flower, Canna hybrida
[Ref.: #5996]	Host species	Canna hybrida
[Ref.: #5996]	Country	Indonesia
[Ref.: #5996]	Country ISO 3 Code	IDN
[Ref.: #5996]	Continent	Asia

[Ref.: #67770]	Sample type/isolated from	Canna flower (Canna hybrida)
[Ref.: #67770]	Host species	Canna hybrida
[Ref.: #67770]	Country	Indonesia
[Ref.: #67770]	Country ISO 3 Code	IDN
[Ref.: #67770]	Continent	Asia

[Ref.: #121435]	Sample type/isolated from	Canna flower
[Ref.: #121435]	Country	Indonesia
[Ref.: #121435]	Country ISO 3 Code	IDN
[Ref.: #121435]	Continent	Asia
[Ref.: #121435]	Isolation date	1996
* marker position based on {}

Isolation sources categories

#Host	#Plants	#Herbaceous plants (Grass,Crops)
#Host Body-Site	#Plant	#Flower

What are isolation sources categories?

[Ref.: #69479]

Global distribution of 16S sequence AB681086 (>99% sequence identity) for Acetobacter orientalis from Microbeatlas

Aquatic Samples	68
Soil Samples	157
Animal Samples	624
Plant Samples	209
Total Samples	1058

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #5996]	Biosafety level	1	Risk group (German classification) According to TRBA 466
[Ref.: #121435]	Biosafety level	1	Risk group (French classification)

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #5996]	Acetobacter orientalis gene for 16S ribosomal RNA, strain: 21F-2	AB052706	1401	GenBank ENA	146474 tax ID
[Ref.: #20218]	Acetobacter orientalis gene for 16S rRNA, partial sequence, strain: NBRC 16606	AB681086	1415	GenBank ENA	146474 tax ID	*

Genome sequence information:

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #67770]	Acetobacter orientalis 21F-2	GCA_000963965	scaffold	GenBank ENA	146474 tax ID
[Ref.: #67770]	Acetobacter orientalis NBRC 16606	GCA_007989335	contig	GenBank ENA	146474 tax ID
[Ref.: #66792]	Acetobacter orientalis 21F-2	1231341.3	wgs	PATRIC	1307913 tax ID	*
[Ref.: #66792]	Acetobacter orientalis strain NBRC 16606	146474.10	wgs	PATRIC	146474 tax ID	*
[Ref.: #66792]	Acetobacter orientalis 21F-2	2899144674	draft	JGI IMG/M	146474 tax ID	*

[Ref.: #67770]

GC-content

52.3 mol%

high performance liquid chromatography (HPLC)

Data predicted using genome information as a basis Genome-based predictions

	Trait	Model	Prediction	Confidence in %	In training data
	Predictions from Diaspora project based on: Acetobacter orientalis 21F-2 PATRIC: 1231341.3
[Ref.: #69480]	gram-positive	gram-positiveⓘ	no	95.833	no
[Ref.: #69480]	anaerobic	anaerobicⓘ	no	88.783	yes
[Ref.: #69480]	spore-forming	spore-formingⓘ	no	92.965	no
[Ref.: #69480]	aerobic	aerobicⓘ	yes	82.825	yes
[Ref.: #69480]	thermophilic	thermophileⓘ	no	98.816	yes
[Ref.: #69480]	flagellated	motile2+ⓘ	yes	59.975	no

Availability in culture collections External links

[Ref.: #5996]

Culture collection no.

DSM 15550, IFO 16606, NBRC 16606, NRIC 0481, JCM 11195, CIP 107379

[Ref.: #69700]

SI-ID 265202

References

#5996

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 15550

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#20218

Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.: StrainInfo introduces electronic passports for microorganisms.. Syst Appl Microbiol. 37: 42 - 50 2014 ( DOI 10.1016/j.syapm.2013.11.002 , PubMed 24321274 )

#33782 ; Curators of the CIP;

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#67770 Japan Collection of Microorganism (JCM) ; Curators of the JCM;

#68382

Automatically annotated from API zym .

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

#69480

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#69700

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID265202.1 )

#121435 Collection of Institut Pasteur ; Curators of the CIP; CIP 107379
* These data were automatically processed and therefore are not curated

Change proposal

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