Name: Nesterenkonia endophytica CCTCC AB 2017176
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 159880

Type strain:

Species: Nesterenkonia endophytica

Culture col. no.: CCTCC AB 2017176, NBRC 112398, EGI 60016

NCBI tax ID(s): 2109272 (species)

Section

Nesterenkonia endophytica CCTCC AB 2017176 is a Gram-positive, coccus-shaped bacterium that forms circular colonies and was isolated from healthy roots of Glycyrrhiza uralensis.

coccus-shaped
colony-forming
Gram-positive
16S sequence
Bacteria

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	07-12-2022 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Actinomycetota*
	Class	*Actinomycetes*
	Order	*Micrococcales*
	Family	*Micrococcaceae*
	Genus	*Nesterenkonia*
	Species	**Nesterenkonia endophytica**
	Full Scientific Name (PNU)	Nesterenkonia endophytica Li et al. 2018

Information on morphological and physiological properties Morphology

[Ref.: #66639]	Gram stain	positive
[Ref.: #66639]	Cell length	0.9 µm
[Ref.: #66639]	Cell shape	coccus-shaped
[Ref.: #66639]	Motility	no

[Ref.: #66639]	Colony color	yellow
[Ref.: #66639]	Colony shape	circular
[Ref.: #66639]	Incubation period	2 days
[Ref.: #66639]	Cultivation medium used	Trypticase Soy Agar (TSA)

Information on culture and growth conditions Culture and growth conditions

[Ref.: #66639]

Culture medium

tryptic soy agar (TSA)

[Ref.: #66639]

Culture medium growth

	Temperatures	Kind of temperature		Temperature
[Ref.: #66639]			optimum	20-25 °C
[Ref.: #66639]			growth	5-30 °C

	pH	Kind of pH		pH	pH range
[Ref.: #66639]			optimum	8
[Ref.: #66639]			growth	7.0-12.0	alkaliphile

Information on physiology and metabolism Physiology and metabolism

[Ref.: #66639]

Ability of spore formation

	Halophily	Salt	Tested relation		Salt conc.
[Ref.: #66639]		NaCl		growth	0-7.5	%(w/v)
[Ref.: #66639]		NaCl		optimum	2.5	%(w/v)

[Ref.: #66639]

Name of tolerated compound

lithium chloride

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #66639]	acetic acid ChEBI	+	carbon source
[Ref.: #66639]	D-glucarate ChEBI	+	carbon source
[Ref.: #66639]	D-gluconate ChEBI	+	carbon source
[Ref.: #66639]	D-glucose ChEBI	+	builds acid from
[Ref.: #66639]	D-lyxose ChEBI	+	builds acid from
[Ref.: #66639]	D-mannitol ChEBI	+	builds acid from
[Ref.: #66639]	D-serine ChEBI	+	carbon source
[Ref.: #66639]	D-xylose ChEBI	+	builds acid from
[Ref.: #66639]	esculin ferric citrate	+	builds acid from
[Ref.: #66639]	formate ChEBI	+	carbon source
[Ref.: #66639]	fructose ChEBI	+	builds acid from
[Ref.: #66639]	gelatin ChEBI	-	hydrolysis
[Ref.: #66639]	inosine ChEBI	+	carbon source
[Ref.: #66639]	L-alanine ChEBI	+	carbon source
[Ref.: #66639]	L-aspartate ChEBI	+	carbon source
[Ref.: #66639]	L-rhamnose ChEBI	+	builds acid from
[Ref.: #66639]	L-serine ChEBI	+	carbon source
[Ref.: #66639]	mannose ChEBI	+	builds acid from
[Ref.: #66639]	n-acetylneuraminate ChEBI	+	carbon source
[Ref.: #66639]	nitrate ChEBI	-	reduction
[Ref.: #66639]	quinate ChEBI	+	carbon source
[Ref.: #66639]	stachyose ChEBI	+	carbon source
[Ref.: #66639]	sucrose ChEBI	+	carbon source
[Ref.: #66639]	trehalose ChEBI	+	builds acid from
[Ref.: #66639]	turanose ChEBI	+	builds acid from
	Only first 10 entries are displayed. Click here to see all.

	Metabolite	Antibiotic sensitivity	Concentration		Antibiotic resistance	Concentration
[Ref.: #66639]	Amikacin ChEBI	yes	30	µg (disc)
[Ref.: #66639]	chloramphenicol ChEBI	yes	30	µg (disc)
[Ref.: #66639]	ciprofloxacin ChEBI	yes	5	µg (disc)
[Ref.: #66639]	erythromycin ChEBI	yes	15	µg (disc)
[Ref.: #66639]	gentamicin ChEBI	yes	10	µg (disc)
[Ref.: #66639]	kanamycin ChEBI	yes	30	µg (disc)
[Ref.: #66639]	netilmycin ChEBI				yes	30	µg (disc)
[Ref.: #66639]	novobiocin ChEBI	yes	5	µg (disc)
[Ref.: #66639]	penicillin g ChEBI	yes	10	Unit (disc)
[Ref.: #66639]	polymyxin b ChEBI	yes	300	Unit (disc)
[Ref.: #66639]	streptomycin ChEBI	yes	10	µg (disc)
[Ref.: #66639]	tetracycline ChEBI	yes	30	µg (disc)
[Ref.: #66639]	tobramycin ChEBI				yes	10	µg (disc)
[Ref.: #66639]	vancomycin ChEBI	yes	30	µg (disc)
	Only first 10 entries are displayed. Click here to see all.

	Metabolite production	Metabolite
[Ref.: #66639]		hydrogen sulfide ChEBI

	Enzyme	Enzyme activity	EC number
[Ref.: #66639]	acid phosphatase	-	3.1.3.2
[Ref.: #66639]	alkaline phosphatase	+	3.1.3.1
[Ref.: #66639]	alpha-chymotrypsin	-	3.4.21.1
[Ref.: #66639]	alpha-glucosidase	+	3.2.1.20
[Ref.: #66639]	alpha-mannosidase	-	3.2.1.24
[Ref.: #66639]	beta-D-fucosidase	-	3.2.1.38
[Ref.: #66639]	beta-galactosidase	+	3.2.1.23
[Ref.: #66639]	beta-glucosidase	-	3.2.1.21
[Ref.: #66639]	beta-glucuronidase	-	3.2.1.31
[Ref.: #66639]	catalase	+	1.11.1.6
[Ref.: #66639]	cystine arylamidase	+	3.4.11.3
[Ref.: #66639]	cytochrome oxidase	-	1.9.3.1
[Ref.: #66639]	esterase (C 4)	+
[Ref.: #66639]	esterase Lipase (C 8)	+
[Ref.: #66639]	leucine arylamidase	+	3.4.11.1
[Ref.: #66639]	lipase (C 14)	-
[Ref.: #66639]	N-acetyl-beta-glucosaminidase	-	3.2.1.52
[Ref.: #66639]	naphthol-AS-BI-phosphohydrolase	+
[Ref.: #66639]	trypsin	-	3.4.21.4
[Ref.: #66639]	urease	-	3.5.1.5
[Ref.: #66639]	valine arylamidase	+
	Only first 10 entries are displayed. Click here to see all.

Fatty acid profile

Metadata FA analysis

Reference

[Ref.: #66639]

Fatty acid	Percentage
C15:0 anteiso	32.8
C17:0 anteiso	22
C15:0 iso	18
C16:0 iso	14.3
C17:0 iso	5.1
C16:0	3.1
C16:1 iso G	1.1

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #66639]	Sample type/isolated from	healthy roots of Glycyrrhiza uralensis
[Ref.: #66639]	Host species	Glycyrrhiza uralensis
[Ref.: #66639]	Geographic location (country and/or sea, region)	Xinyuan County, Xinjiang Province
[Ref.: #66639]	Country	China
[Ref.: #66639]	Country ISO 3 Code	CHN
[Ref.: #66639]	Continent	Asia
[Ref.: #66639]	Geographic location	43.3900°/83.8400°
[Ref.: #66639]	Enrichment culture	R2A
[Ref.: #66639]	Enrichment culture duration	7-28 days
[Ref.: #66639]	Enrichment culture temperature	30 ̊C
[Ref.: #66639]	Isolation/enrichment procedure	The roots were washed with tap water followed by surface-sterilization. The root samples were then sliced into small pieces and plated.
* marker position based on {}

Isolation sources categories

#Host	#Plants	#Herbaceous plants (Grass,Crops)
#Host Body-Site	#Plant	#Root (Rhizome)

What are isolation sources categories?

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #66639]	Nesterenkonia endophyticus strain EGI 60016 16S ribosomal RNA gene, partial sequence	MF630917	1487	ENA	2109272 tax ID

[Ref.: #66639]

GC-content

64.10 mol%

high performance liquid chromatography (HPLC)

Availability in culture collections External links

[Ref.: #66639]

Culture collection no.

CCTCC AB 2017176, NBRC 112398, EGI 60016

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Nesterenkonia endophytica sp. nov., isolated from roots of Glycyrrhiza uralensis.	Li L, Li YQ, Fu YS, Zhang H, Alkhalifah DHM, Salam N, Hozzein WN, Asem MD, Li WJ	Int J Syst Evol Microbiol	10.1099/ijsem.0.002905	2018	*

References

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#66639

Li Li, Yan-Qiong Li, Yu-Song Fu, Hui Zhang, Dalal Hussien M. Alkhalifah, Nimaichand Salam, Wael N. Hozzein, Mipeshwaree Devi Asem and Wen-Jun Li: Nesterenkonia endophytica sp. nov., isolated from roots of Glycyrrhiza uralensis. IJSEM 68: 2659 - 2663 2018 ( DOI 10.1099/ijsem.0.002905 , PubMed 29969086 )

* These data were automatically processed and therefore are not curated

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