Name: Zhouia spongiae HN-Y44
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 159859

Type strain:

Species: Zhouia spongiae

Strain Designation: HN-Y44

Culture col. no.: MCCC 1K03329, LMG 30460

NCBI tax ID(s): 2202721 (species)

Section

Zhouia spongiae HN-Y44 is an aerobe, mesophilic, Gram-negative bacterium that forms circular colonies and was isolated from marine sponge sample Mycale phyllophila.

colony-forming
Gram-negative
rod-shaped
aerobe
mesophilic
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Bacteroidota*
	Class	*Flavobacteriia*
	Order	*Flavobacteriales*
	Family	*Flavobacteriaceae*
	Genus	*Zhouia*
	Species	**Zhouia spongiae**
	Full Scientific Name (LPSN)	Zhouia spongiae Zhuang et al. 2018

Information on morphological and physiological properties Morphology

[Ref.: #66575]	Gram stain	negative

[Ref.: #66575]	Cell length	1.8-4.6 µm

[Ref.: #66575]	Cell width	0.2-0.3 µm

[Ref.: #66575]	Cell shape	rod-shaped

[Ref.: #66575]	Motility	no

[Ref.: #66575]	Colony size	1-2 mm
[Ref.: #66575]	Colony color	pale-yellow
[Ref.: #66575]	Colony shape	circular
[Ref.: #66575]	Incubation period	3 days
[Ref.: #66575]	Cultivation medium used	Marine agar (MA)

[Ref.: #66575]	Pigment production	no
[Ref.: #66575]	Pigment name	flexirubin-type

Information on culture and growth conditions Culture and growth conditions

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Culture medium

Marine agar (MA)

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Culture medium growth

	Temperatures	Kind of temperature		Temperature
[Ref.: #66575]			optimum	28 °C
[Ref.: #66575]			growth	10-40 °C

[Ref.: #66575]

Temperature range

mesophilic

	pH	Kind of pH		pH
[Ref.: #66575]			optimum	7-8
[Ref.: #66575]			growth	6-10

Information on physiology and metabolism Physiology and metabolism

[Ref.: #66575]

Oxygen tolerance

aerobe

	Halophily	Salt	Tested relation		Salt conc.
[Ref.: #66575]		NaCl		growth	0-8	%(w/v)
[Ref.: #66575]		NaCl		optimum	2-5	%(w/v)

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #66575]	adipate ChEBI	-	assimilation
[Ref.: #66575]	amygdalin ChEBI	-	builds acid from
[Ref.: #66575]	casein	-	hydrolysis
[Ref.: #66575]	citric acid ChEBI	-	assimilation
[Ref.: #66575]	D-glucose ChEBI	+	assimilation
[Ref.: #66575]	D-glucose ChEBI	-	builds acid from
[Ref.: #66575]	D-glucose ChEBI	-	fermentation
[Ref.: #66575]	D-mannitol ChEBI	-	assimilation
[Ref.: #66575]	D-mannitol ChEBI	-	builds acid from
[Ref.: #66575]	D-mannose ChEBI	+	assimilation
[Ref.: #66575]	D-sorbitol ChEBI	-	builds acid from
[Ref.: #66575]	decanoate ChEBI	-	assimilation
[Ref.: #66575]	esculin ChEBI	+	hydrolysis
[Ref.: #66575]	gelatin ChEBI	-	hydrolysis
[Ref.: #66575]	L-arabinose ChEBI	-	assimilation
[Ref.: #66575]	L-arabinose ChEBI	-	builds acid from
[Ref.: #66575]	L-rhamnose ChEBI	-	builds acid from
[Ref.: #66575]	malate ChEBI	-	assimilation
[Ref.: #66575]	maltose ChEBI	+	assimilation
[Ref.: #66575]	melibiose ChEBI	-	builds acid from
[Ref.: #66575]	myo-inositol ChEBI	-	builds acid from
[Ref.: #66575]	N-acetylglucosamine ChEBI	+	assimilation
[Ref.: #66575]	nitrate ChEBI	+	reduction
[Ref.: #66575]	phenylacetate ChEBI	-	assimilation
[Ref.: #66575]	potassium gluconate ChEBI	-	assimilation
[Ref.: #66575]	sodium citrate ChEBI	-	assimilation
[Ref.: #66575]	starch ChEBI	-	hydrolysis
[Ref.: #66575]	sucrose ChEBI	-	builds acid from
[Ref.: #66575]	tween 20 ChEBI	+	hydrolysis
[Ref.: #66575]	tween 40 ChEBI	+	hydrolysis
[Ref.: #66575]	tween 80 ChEBI	+	hydrolysis
	Only first 10 entries are displayed. Click here to see all.

	Metabolite	Production
[Ref.: #66575]	acetoin ChEBI	yes
[Ref.: #66575]	hydrogen sulfide ChEBI	no
[Ref.: #66575]	indole ChEBI	no

	Physiological tests	Metabolite	Voges-Proskauer-test
[Ref.: #66575]		acetoin ChEBI	+

	Enzyme	Enzyme activity	EC number
[Ref.: #66575]	acid phosphatase	+	3.1.3.2
[Ref.: #66575]	alkaline phosphatase	+	3.1.3.1
[Ref.: #66575]	alpha-chymotrypsin	-	3.4.21.1
[Ref.: #66575]	alpha-fucosidase	-	3.2.1.51
[Ref.: #66575]	alpha-galactosidase	+/-	3.2.1.22
[Ref.: #66575]	alpha-glucosidase	+	3.2.1.20
[Ref.: #66575]	alpha-mannosidase	+	3.2.1.24
[Ref.: #66575]	arginine dihydrolase	-	3.5.3.6
[Ref.: #66575]	beta-galactosidase	-	3.2.1.23
[Ref.: #66575]	beta-glucosidase	+	3.2.1.21
[Ref.: #66575]	beta-glucuronidase	-	3.2.1.31
[Ref.: #66575]	catalase	+	1.11.1.6
[Ref.: #66575]	cystine aminopeptidase	+/-	3.4.11.3
[Ref.: #66575]	cytochrome oxidase	+	1.9.3.1
[Ref.: #66575]	esterase (C 4)	+
[Ref.: #66575]	esterase Lipase (C 8)	+/-
[Ref.: #66575]	leucine aminopeptidase	+	3.4.1.1
[Ref.: #66575]	lipase (C 14)	+/-
[Ref.: #66575]	lysine decarboxylase	-	4.1.1.18
[Ref.: #66575]	N-acetyl-beta-glucosaminidase	+	3.2.1.52
[Ref.: #66575]	naphthol-AS-BI-phosphohydrolase	+
[Ref.: #66575]	ornithine decarboxylase	-	4.1.1.17
[Ref.: #66575]	trypsin	+	3.4.21.4
[Ref.: #66575]	tryptophan deaminase	-	4.1.99.1
[Ref.: #66575]	urease	-	3.5.1.5
[Ref.: #66575]	valine aminopeptidase	+
	Only first 10 entries are displayed. Click here to see all.

Fatty acid profile

Metadata FA analysis

Reference

[Ref.: #66575]

Fatty acid	Percentage
C15:0 iso	26.8
C15:1 iso G	25.6
C17:0 iso 3OH	9.8
C15:0 anteiso	5.7
C16:1 w7c/ C16:1 w6c	4.4
C15:0 iso 3OH	3.8
C16:0 iso 3OH	3.5
C16:0 iso	3.1
C16:0	1.9
C15:1 anteiso A	1.9
C16:1 iso G	1.6
C13:1 at 12-13	1.4

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #66575]	Sample type/isolated from	marine sponge sample Mycale phyllophila
[Ref.: #66575]	Host species	Mycale phyllophila
[Ref.: #66575]	Sampling date	2016-06
[Ref.: #66575]	Geographic location (country and/or sea, region)	Yangpu Bay, Hainan
[Ref.: #66575]	Country	China
[Ref.: #66575]	Country ISO 3 Code	CHN
[Ref.: #66575]	Continent	Asia
[Ref.: #66575]	Geographic location	19.6833°/109.1000°
[Ref.: #66575]	Enrichment culture	YPG agar
[Ref.: #66575]	Enrichment culture composition	1 % yeast extract, 1 % peptone, 2 % glucose and 1.5 % agar; seawater
[Ref.: #66575]	Enrichment culture duration	14 days
[Ref.: #66575]	Enrichment culture temperature	28 ̊C
* marker position based on {}

Isolation sources categories

#Environmental	#Aquatic	#Marine
#Host	#Invertebrates (Other)	#Porifera (Sponges)

What are isolation sources categories?

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #66575]	Zhouia sp. strain HN-Y44 16S ribosomal RNA gene, partial sequence	MG437234	1428	ENA	2202721 tax ID

Genome sequence information:

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #66792]	Zhouia spongiae HN-Y44	GCA_022760175	complete	GenBank	2202721 tax ID	*

[Ref.: #66575]

GC-content

32.90 mol%

high performance liquid chromatography (HPLC)

Data predicted using genome information as a basis Genome-based predictions

	Trait	Prediction	Confidence in %	In training data
	Predictions from Diaspora project based on: Zhouia spongiae HN-Y44 NCBI: GCA_022760175.1
[Ref.: #69480]	flagellated	no	95.798	no
[Ref.: #69480]	gram-positive	no	98.49	no
[Ref.: #69480]	anaerobic	no	99.654	yes
[Ref.: #69480]	aerobic	yes	88.14	yes
[Ref.: #69480]	halophile	no	76.431	no
[Ref.: #69480]	spore-forming	no	96.566	no
[Ref.: #69480]	motile	no	91.39	yes
[Ref.: #69480]	glucose-ferment	no	89.475	yes
[Ref.: #69480]	thermophile	no	98.384	yes
[Ref.: #69480]	glucose-util	yes	89.286	yes

Availability in culture collections External links

[Ref.: #66575]

Culture collection no.

MCCC 1K03329, LMG 30460

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Zhouia spongiae sp. nov., isolated from a marine sponge.	Zhuang L, Lin B, Qin F, Luo L	Int J Syst Evol Microbiol	10.1099/ijsem.0.002808	2018	*
Phylogeny	Description of Abyssalbus ytuae gen. nov., sp. nov., a novel member of the family Flavobacteriaceae isolated from the sediment of the Mariana Trench.	Xu X, Zhang S, Sun X, Xu X, Zhang J	Int J Syst Evol Microbiol	10.1099/ijsem.0.005459	2022	*

References

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#66575

Lingping Zhuang, Binbin Lin, Fei Qin and Lianzhong Luo: Zhouia spongiae sp. nov., isolated from a marine sponge. IJSEM 68: 2194 - 2198 2018 ( DOI 10.1099/ijsem.0.002808 , PubMed 29781798 )

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#69480

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

* These data were automatically processed and therefore are not curated

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