Name: Planococcus halotolerans CGMCC 1.13628
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 159841

Type strain:

Species: Planococcus halotolerans

Culture col. no.: CGMCC 1.13628, KCTC 43001, SCU 63

NCBI tax ID(s): 2233542 (species)

Section

Planococcus halotolerans CGMCC 1.13628 is an aerobe, Gram-positive, motile bacterium that forms circular colonies and was isolated from saline soil sample.

colony-forming
Gram-positive
motile
rod-shaped
aerobe
Bacteria

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	07-12-2022 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Bacillota*
	Class	*Bacilli*
	Order	*Caryophanales*
	Family	*Caryophanaceae*
	Genus	*Planococcus*
	Species	**Planococcus halotolerans**
	Full Scientific Name (PNU)	Planococcus halotolerans Gan et al. 2018

Information on morphological and physiological properties Morphology

[Ref.: #66733]	Gram stain	positive
[Ref.: #66733]	Cell length	0.4-1.6 µm
[Ref.: #66733]	Cell width	0.4-0.6 µm
[Ref.: #66733]	Cell shape	rod-shaped
[Ref.: #66733]	Motility	yes

[Ref.: #66733]	Colony size	2-3 µm
[Ref.: #66733]	Colony color	orange
[Ref.: #66733]	Colony shape	circular
[Ref.: #66733]	Incubation period	2 days
[Ref.: #66733]	Cultivation medium used	LB agar

Information on culture and growth conditions Culture and growth conditions

[Ref.: #66733]

Culture medium

LB (Luria-Bertani) agar

[Ref.: #66733]

Culture medium growth

	Temperatures	Kind of temperature		Temperature
[Ref.: #66733]			growth	0-40 °C
[Ref.: #66733]			optimum	30-35 °C

[Ref.: #66733]

Temperature range

mesophilic

	pH	Kind of pH		pH
[Ref.: #66733]			optimum	7.0-7.5
[Ref.: #66733]			growth	6.5-9.0

Information on physiology and metabolism Physiology and metabolism

[Ref.: #66733]

Oxygen tolerance

aerobe

[Ref.: #66733]

Halophily / tolerance level

halotolerant

	Halophily	Salt	Tested relation		Salt conc.
[Ref.: #66733]		NaCl		growth	0-15	%(w/v)
[Ref.: #66733]		NaCl		optimum	3	%(w/v)

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #66733]	2-hydroxybutyrate ChEBI	-	oxidation
[Ref.: #66733]	4-hydroxybutyrate ChEBI	-	oxidation
[Ref.: #66733]	acetic acid ChEBI	+	oxidation
[Ref.: #66733]	acetoacetate ChEBI	+	oxidation
[Ref.: #66733]	alpha-D-glucose ChEBI	+	oxidation
[Ref.: #66733]	casein	+	hydrolysis
[Ref.: #66733]	cellobiose ChEBI	+	builds acid from
[Ref.: #66733]	cellobiose ChEBI	+	oxidation
[Ref.: #66733]	D-arabitol ChEBI	+	oxidation
[Ref.: #66733]	D-fructose ChEBI	+	oxidation
[Ref.: #66733]	D-fructose 6-phosphate ChEBI	-	oxidation
[Ref.: #66733]	D-galactonic acid lactone ChEBI	-	oxidation
[Ref.: #66733]	D-galactose ChEBI	+	oxidation
[Ref.: #66733]	D-glucose 6-phosphate ChEBI	+	oxidation
[Ref.: #66733]	D-mannitol ChEBI	-	builds acid from
[Ref.: #66733]	D-mannitol ChEBI	+	oxidation
[Ref.: #66733]	D-mannose ChEBI	+	oxidation
[Ref.: #66733]	D-sorbitol ChEBI	-	builds acid from
[Ref.: #66733]	D-sorbitol ChEBI	-	oxidation
[Ref.: #66733]	D-xylose ChEBI	-	builds acid from
[Ref.: #66733]	dextrin ChEBI	+	oxidation
[Ref.: #66733]	galactitol ChEBI	-	builds acid from
[Ref.: #66733]	galactose ChEBI	+	builds acid from
[Ref.: #66733]	gelatin ChEBI	-	hydrolysis
[Ref.: #66733]	gelatin ChEBI	+	oxidation
[Ref.: #66733]	gentiobiose ChEBI	+	oxidation
[Ref.: #66733]	glucose ChEBI	+	builds acid from
[Ref.: #66733]	glycerol ChEBI	-	builds acid from
[Ref.: #66733]	glycerol ChEBI	+	oxidation
[Ref.: #66733]	L-arabinose ChEBI	-	builds acid from
[Ref.: #66733]	L-aspartate ChEBI	+	oxidation
[Ref.: #66733]	L-fructose ChEBI	+	oxidation
[Ref.: #66733]	L-glutamate ChEBI	+	oxidation
[Ref.: #66733]	L-pyroglutamic acid ChEBI	+	oxidation
[Ref.: #66733]	L-serine ChEBI	+	oxidation
[Ref.: #66733]	maltose ChEBI	+	builds acid from
[Ref.: #66733]	maltose ChEBI	+	oxidation
[Ref.: #66733]	mannose ChEBI	-	builds acid from
[Ref.: #66733]	melibiose ChEBI	-	oxidation
[Ref.: #66733]	methyl pyruvate ChEBI	-	oxidation
[Ref.: #66733]	myo-inositol ChEBI	-	builds acid from
[Ref.: #66733]	myo-inositol ChEBI	+	oxidation
[Ref.: #66733]	N-acetyl-beta-D-mannosamine ChEBI	+	oxidation
[Ref.: #66733]	N-acetylglucosamine ChEBI	+	oxidation
[Ref.: #66733]	nitrate ChEBI	-	reduction
[Ref.: #66733]	pectin ChEBI	-	oxidation
[Ref.: #66733]	salicin ChEBI	+	oxidation
[Ref.: #66733]	starch ChEBI	-	builds acid from
[Ref.: #66733]	starch ChEBI	-	hydrolysis
[Ref.: #66733]	sucrose ChEBI	+	builds acid from
[Ref.: #66733]	sucrose ChEBI	+	oxidation
[Ref.: #66733]	trehalose ChEBI	+	oxidation
[Ref.: #66733]	turanose ChEBI	+	oxidation
[Ref.: #66733]	tween 20 ChEBI	+	hydrolysis
[Ref.: #66733]	tween 40 ChEBI	-	hydrolysis
[Ref.: #66733]	tween 40 ChEBI	+	oxidation
[Ref.: #66733]	tween 60 ChEBI	+	hydrolysis
[Ref.: #66733]	tween 80 ChEBI	-	hydrolysis
[Ref.: #66733]	urea ChEBI	-	hydrolysis
	Only first 10 entries are displayed. Click here to see all.

	Metabolite	Antibiotic sensitivity	Concentration		Antibiotic resistance	Concentration
[Ref.: #66733]	amoxicillin ChEBI	yes	10	µg (disc)
[Ref.: #66733]	ampicillin ChEBI				yes	10	µg (disc)
[Ref.: #66733]	carbenicillin ChEBI	yes	100	µg (disc)
[Ref.: #66733]	ceftazidime ChEBI				yes	30	µg (disc)
[Ref.: #66733]	cephalexin ChEBI	yes	30	µg (disc)
[Ref.: #66733]	chloramphenicol ChEBI	yes	30	µg (disc)
[Ref.: #66733]	erythromycin ChEBI	yes	15	µg (disc)
[Ref.: #66733]	gentamicin ChEBI	yes	10	µg (disc)
[Ref.: #66733]	kanamycin ChEBI	yes	30	µg (disc)
[Ref.: #66733]	nalidixic acid ChEBI	yes	30	µg (disc)
[Ref.: #66733]	neomycin ChEBI	yes	30	µg (disc)
[Ref.: #66733]	nitrofurantoin ChEBI	yes	300	µg (disc)
[Ref.: #66733]	oxacillin ChEBI	yes	1	µg (disc)
[Ref.: #66733]	penicillin ChEBI	yes	10	Unit (disc)
[Ref.: #66733]	polymyxin b ChEBI	yes	30	µg (disc)
[Ref.: #66733]	streptomycin ChEBI	yes	10	µg (disc)
[Ref.: #66733]	tetracycline ChEBI	yes	30	µg (disc)
	Only first 10 entries are displayed. Click here to see all.

	Metabolite production	Metabolite
[Ref.: #66733]		hydrogen sulfide ChEBI
[Ref.: #66733]		indole ChEBI

	Enzyme	Enzyme activity	EC number
[Ref.: #66733]	acid phosphatase	-	3.1.3.2
[Ref.: #66733]	alkaline phosphatase	+	3.1.3.1
[Ref.: #66733]	alpha-chymotrypsin	+	3.4.21.1
[Ref.: #66733]	alpha-fucosidase	-	3.2.1.51
[Ref.: #66733]	alpha-galactosidase	-	3.2.1.22
[Ref.: #66733]	alpha-glucosidase	+	3.2.1.20
[Ref.: #66733]	alpha-mannosidase	-	3.2.1.24
[Ref.: #66733]	beta-galactosidase	-	3.2.1.23
[Ref.: #66733]	beta-glucosidase	-	3.2.1.21
[Ref.: #66733]	beta-glucuronidase	-	3.2.1.31
[Ref.: #66733]	catalase	+	1.11.1.6
[Ref.: #66733]	cystine arylamidase	+	3.4.11.3
[Ref.: #66733]	cytochrome oxidase	+	1.9.3.1
[Ref.: #66733]	esterase (C 4)	+
[Ref.: #66733]	esterase Lipase (C 8)	+
[Ref.: #66733]	leucine arylamidase	+	3.4.11.1
[Ref.: #66733]	lipase (C 14)	-
[Ref.: #66733]	N-acetyl-beta-glucosaminidase	-	3.2.1.52
[Ref.: #66733]	naphthol-AS-BI-phosphohydrolase	+
[Ref.: #66733]	trypsin	-	3.4.21.4
[Ref.: #66733]	valine arylamidase	+
	Only first 10 entries are displayed. Click here to see all.

Fatty acid profile

Metadata FA analysis

Reference

[Ref.: #66733]

Fatty acid	Percentage
C15:0 anteiso	39.7
C16:1 w7c alcohol	12.9
C16:0 iso	10.6
C14:0 iso	10
C16:0	6.3
C17:0 anteiso	5.6
C15:0 iso	5.4
C17:0	3.7
C18:1 w9c	2.7
C18:0	1.8
C18:0 iso	0.8
C16:1 w9c	0.8

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #66733]	Sample type/isolated from	saline soil sample
[Ref.: #66733]	Geographic location (country and/or sea, region)	Yadan National Geological Park, Gansu Province, in north-western China
[Ref.: #66733]	Country	China
[Ref.: #66733]	Country ISO 3 Code	CHN
[Ref.: #66733]	Continent	Asia
[Ref.: #66733]	Geographic location	40.4612°/92.9989°
[Ref.: #66733]	Enrichment culture	modified saline agar medium
[Ref.: #66733]	Enrichment culture composition	containing, per litre, 7.5 g casein peptone, 100 g NaCl, 10 g yeast extract, 1.5 ml MS mixture (0.05 g each of betaine, proline, glycine, D-sorbitol and glutamate, added to 1000 ml H2O) and 20 g agar
[Ref.: #66733]	Enrichment culture duration	3 days
[Ref.: #66733]	Enrichment culture temperature	37 ̊C
[Ref.: #66733]	Isolation/enrichment procedure	was isolated from a saline soil sample collected from Yadan National Geological Park, Gansu Province, in north-western China. To obtain halotolerant and halophilic bacteria, the sample was serially diluted with 0.9 % (w/v) physiological saline solution and then spread on a modified saline agar medium containing, per litre, 7.5 g casein peptone, 100 g NaCl, 10 g yeast extract, 1.5 ml MS mixture (0.05 g each of betaine, proline, glycine, D-sorbitol and glutamate, added to 1000 ml H2O) and 20 g agar (adjusted to pH 8.0 with HCl/NaOH) and incubated at 37°C for 3 days.
* marker position based on {}

Isolation sources categories

#Condition	#Saline	-
#Environmental	#Terrestrial	#Soil

What are isolation sources categories?

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

[Ref.: #66733]

GC-content

44.6 mol%

genome sequence analysis

Availability in culture collections External links

[Ref.: #66733]

Culture collection no.

CGMCC 1.13628, KCTC 43001, SCU 63

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Planococcus soli sp. nov., isolated from antarctic soil.	Zhang S, Ping W, Xin Y, Xin D, Zhang J	Antonie Van Leeuwenhoek	10.1007/s10482-021-01581-z	2021	*
Genetics	Genomic insights into the salt tolerance and cold adaptation of Planococcus halotolerans SCU63(T).	Gan L, Li X, Tian Y, Peng B	Arch Microbiol	10.1007/s00203-020-01979-9	2020	*
Phylogeny	Characteristics of Planococcus antioxidans sp. nov., an antioxidant-producing strain isolated from the desert soil in the Qinghai-Tibetan Plateau.	Zhang B, Yang R, Zhang G, Liu Y, Zhang D, Zhang W, Chen T, Liu G	Microbiologyopen	10.1002/mbo3.1028	2020	*
Phylogeny	Planococcus halotolerans sp. nov., isolated from a saline soil sample in China.	Gan L, Zhang Y, Zhang L, Li X, Wang Z, He L, Li Z, Tian Y	Int J Syst Evol Microbiol	10.1099/ijsem.0.003019	2018	*

References

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#66733

Longzhan Gan, Yi Zhang, Lingli Zhang, Xiaoguang Li, Zhikuan Wang, Linling He, Zhenjiang Li, Yongqiang Tian: Planococcus halotolerans sp. nov., isolated from a saline soil sample in China. IJSEM 68: 3500 - 3505 2018 ( DOI 10.1099/ijsem.0.003019 , PubMed 30265231 )

* These data were automatically processed and therefore are not curated

Change proposal

You found an error in BacDive? Please tell us about it!

Note that changes will be reviewed and judged. If your changes are legitimate, changes will occur within the next BacDive update. Only proposed changes supported by the according reference will be reviewed. The BacDive team reserves the right to reject proposed changes.

Search for species Planococcus halotolerans in external resources:
SILVA
BRENDA
PANGAEA
StrainInfo
GBIF