Name: Psychromicrobium lacuslunae JCM 31143
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 159836

Type strain:

Species: Psychromicrobium lacuslunae

Culture col. no.: JCM 31143, KACC 19070, MCC 2780, MTCC 12460, IHBB 11108

Strain history: A. Gulati; CSIR-Inst. of Himalayan Bioresour. Technol., India; IHBB 11108.

NCBI tax ID(s): 1618207 (species)

Section

Psychromicrobium lacuslunae JCM 31143 is an aerobe, mesophilic, Gram-positive bacterium that was isolated from during bacterial diversity analysis from the subsurface water of Chandra Tal Lake.

Gram-positive
aerobe
mesophilic
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Actinomycetota*
	Class	*Actinomycetes*
	Order	*Micrococcales*
	Family	*Micrococcaceae*
	Genus	*Psychromicrobium*
	Species	**Psychromicrobium lacuslunae**
	Full Scientific Name (LPSN)	Psychromicrobium lacuslunae Kiran et al. 2018

Information on morphological and physiological properties Morphology

[Ref.: #66714]	Gram stain	positive

[Ref.: #66714]	Cell length	1.1-1.25 µm

[Ref.: #66714]	Cell width	0.5-0.8 µm

[Ref.: #66714]	Motility	no

[Ref.: #66714]	Colony color	creamy
[Ref.: #66714]	Incubation period	1 day
[Ref.: #66714]	Cultivation medium used	Trypticase Soy Agar (TSA)

Information on culture and growth conditions Culture and growth conditions

[Ref.: #66714]

Culture medium

tryptic soy agar (TSA)

[Ref.: #66714]

Culture medium growth

		Temperature
[Ref.: #66714]	optimum	28 °C
[Ref.: #66714]	growth	5-37 °C
[Ref.: #67770]	growth	28 °C

[Ref.: #66714]	Temperature range	mesophilic
[Ref.: #67770]	Temperature range	mesophilic

	pH	Kind of pH		pH
[Ref.: #66714]			optimum	7
[Ref.: #66714]			growth	5-12

Information on physiology and metabolism Physiology and metabolism

[Ref.: #66714]

Oxygen tolerance

aerobe

	Halophily	Salt	Tested relation		Salt conc.
[Ref.: #66714]		NaCl		growth	0-8	%(w/v)
[Ref.: #66714]		NaCl		optimum	1	%(w/v)

[Ref.: #67770]

Observation

quinones: MK-9(H₂), MK-10(H₂)

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #66714]	(S)-lactate ChEBI	+	carbon source
[Ref.: #66714]	1 % sodium lactate	+	carbon source
[Ref.: #66714]	2-oxobutanoate ChEBI	-	carbon source
[Ref.: #66714]	2-oxoglutarate ChEBI	-	carbon source
[Ref.: #66714]	3-hydroxybutyrate ChEBI	+	carbon source
[Ref.: #66714]	3-O-methyl-D-glucose ChEBI	-	carbon source
[Ref.: #66714]	4-hydroxyphenylacetic acid ChEBI	+	carbon source
[Ref.: #66714]	acetic acid ChEBI	+	carbon source
[Ref.: #66714]	acetoacetate ChEBI	+	carbon source
[Ref.: #66714]	adipate ChEBI	+	assimilation
[Ref.: #66714]	alpha-D-glucose ChEBI	+	carbon source
[Ref.: #66714]	alpha-hydroxybutyrate	+	carbon source
[Ref.: #66714]	arginine ChEBI	+	hydrolysis
[Ref.: #66714]	aztreonam ChEBI	+	carbon source
[Ref.: #66714]	bromosuccinate ChEBI	+	carbon source
[Ref.: #66714]	casein	+	hydrolysis
[Ref.: #66714]	cellobiose ChEBI	-	carbon source
[Ref.: #66714]	cellulose ChEBI	-	hydrolysis
[Ref.: #66714]	citric acid ChEBI	+	carbon source
[Ref.: #66714]	D-arabitol ChEBI	+	carbon source
[Ref.: #66714]	D-aspartate ChEBI	-	carbon source
[Ref.: #66714]	D-fructose ChEBI	+	carbon source
[Ref.: #66714]	D-fructose 6-phosphate ChEBI	-	carbon source
[Ref.: #66714]	D-fucose ChEBI	-	carbon source
[Ref.: #66714]	D-galactose ChEBI	+	carbon source
[Ref.: #66714]	D-galacturonic acid ChEBI	-	carbon source
[Ref.: #66714]	D-glucarate ChEBI	-	carbon source
[Ref.: #66714]	D-gluconate ChEBI	+	assimilation
[Ref.: #66714]	D-glucose ChEBI	-	assimilation
[Ref.: #66714]	D-glucose 6-phosphate ChEBI	+	carbon source
[Ref.: #66714]	D-glucuronate ChEBI	-	carbon source
[Ref.: #66714]	D-glucuronate ChEBI	+	carbon source
[Ref.: #66714]	D-malate ChEBI	+	carbon source
[Ref.: #66714]	D-mannitol ChEBI	-	assimilation
[Ref.: #66714]	D-mannitol ChEBI	+	carbon source
[Ref.: #66714]	D-mannose ChEBI	-	assimilation
[Ref.: #66714]	D-mannose ChEBI	+	carbon source
[Ref.: #66714]	D-serine ChEBI	-	carbon source
[Ref.: #66714]	D-sorbitol ChEBI	+	carbon source
[Ref.: #66714]	D-trehalose ChEBI	+	carbon source
[Ref.: #66714]	decanoate ChEBI	-	assimilation
[Ref.: #66714]	dextrin ChEBI	+	carbon source
[Ref.: #66714]	esculin ChEBI	+	hydrolysis
[Ref.: #66714]	formate ChEBI	+	carbon source
[Ref.: #66714]	fusidate ChEBI	-	carbon source
[Ref.: #66714]	galactarate ChEBI	-	carbon source
[Ref.: #66714]	gamma-aminobutyric acid ChEBI	-	carbon source
[Ref.: #66714]	gelatin ChEBI	+	carbon source
[Ref.: #66714]	gelatin ChEBI	+	hydrolysis
[Ref.: #66714]	gentiobiose ChEBI	+	carbon source
[Ref.: #66714]	glucuronamide ChEBI	-	carbon source
[Ref.: #66714]	glycerol ChEBI	+	carbon source
[Ref.: #66714]	glycine-proline ChEBI	+	carbon source
[Ref.: #66714]	guanidinium chloride ChEBI	+	assimilation
[Ref.: #66714]	inosine ChEBI	+	carbon source
[Ref.: #66714]	L-alanine ChEBI	+	carbon source
[Ref.: #66714]	L-arabinose ChEBI	-	assimilation
[Ref.: #66714]	L-arginine ChEBI	+	carbon source
[Ref.: #66714]	L-aspartate ChEBI	-	carbon source
[Ref.: #66714]	L-fucose ChEBI	+	carbon source
[Ref.: #66714]	L-galactonic acid gamma-lactone ChEBI	-	carbon source
[Ref.: #66714]	L-glutamate ChEBI	+	carbon source
[Ref.: #66714]	L-histidine ChEBI	+	carbon source
[Ref.: #66714]	L-malate ChEBI	+	carbon source
[Ref.: #66714]	L-pyroglutamic acid ChEBI	+	carbon source
[Ref.: #66714]	L-rhamnose ChEBI	-	carbon source
[Ref.: #66714]	L-serine ChEBI	+	carbon source
[Ref.: #66714]	lactose ChEBI	+	carbon source
[Ref.: #66714]	lithium chloride ChEBI	+	carbon source
[Ref.: #66714]	malate ChEBI	-	assimilation
[Ref.: #66714]	maltose ChEBI	-	assimilation
[Ref.: #66714]	maltose ChEBI	+	carbon source
[Ref.: #66714]	melibiose ChEBI	+	carbon source
[Ref.: #66714]	methyl (R)-lactate ChEBI	+	carbon source
[Ref.: #66714]	methyl beta-D-glucopyranoside ChEBI	-	carbon source
[Ref.: #66714]	methyl pyruvate ChEBI	-	carbon source
[Ref.: #66714]	myo-inositol ChEBI	+	carbon source
[Ref.: #66714]	N-acetyl-D-mannosamine ChEBI	-	carbon source
[Ref.: #66714]	N-acetylgalactosamine ChEBI	+	carbon source
[Ref.: #66714]	N-acetylglucosamine ChEBI	-	assimilation
[Ref.: #66714]	N-acetylglucosamine ChEBI	+	carbon source
[Ref.: #66714]	n-acetylneuraminate ChEBI	-	carbon source
[Ref.: #66714]	nalidixic acid ChEBI	+	carbon source
[Ref.: #66714]	nitrate ChEBI	-	reduction
[Ref.: #66714]	pectin ChEBI	+	carbon source
[Ref.: #66714]	phenylacetate ChEBI	+	assimilation
[Ref.: #66714]	potassium gluconate ChEBI	+	assimilation
[Ref.: #66714]	potassium tellurite ChEBI	-	carbon source
[Ref.: #66714]	propionate ChEBI	+	carbon source
[Ref.: #66714]	quinate ChEBI	-	carbon source
[Ref.: #66714]	raffinose ChEBI	+	carbon source
[Ref.: #66714]	salicin ChEBI	+	carbon source
[Ref.: #66714]	sodium bromate ChEBI	+	carbon source
[Ref.: #66714]	sodium butyrate ChEBI	+	carbon source
[Ref.: #66714]	sodium citrate ChEBI	+	assimilation
[Ref.: #66714]	stachyose ChEBI	-	carbon source
[Ref.: #66714]	starch ChEBI	-	hydrolysis
[Ref.: #66714]	sucrose ChEBI	+	carbon source
[Ref.: #66714]	tetrazolium blue ChEBI	+	assimilation
[Ref.: #66714]	turanose ChEBI	+	carbon source
[Ref.: #66714]	tween 20 ChEBI	+	hydrolysis
[Ref.: #66714]	tween 40 ChEBI	+	carbon source
[Ref.: #66714]	tween 40 ChEBI	+	hydrolysis
[Ref.: #66714]	tween 80 ChEBI	-	hydrolysis
	Only first 10 entries are displayed. Click here to see all.

	Metabolite production	Metabolite	Production
[Ref.: #66714]		indole-3-acetic acid ChEBI	no

	Enzyme	Enzyme activity	EC number
[Ref.: #66714]	acid phosphatase	+	3.1.3.2
[Ref.: #66714]	alkaline phosphatase	-	3.1.3.1
[Ref.: #66714]	alpha-chymotrypsin	-	3.4.21.1
[Ref.: #66714]	alpha-fucosidase	-	3.2.1.51
[Ref.: #66714]	alpha-galactosidase	-	3.2.1.22
[Ref.: #66714]	alpha-glucosidase	+	3.2.1.20
[Ref.: #66714]	alpha-mannosidase	+	3.2.1.24
[Ref.: #66714]	beta-galactosidase	+	3.2.1.23
[Ref.: #66714]	beta-glucosidase	+	3.2.1.21
[Ref.: #66714]	beta-glucuronidase	-	3.2.1.31
[Ref.: #66714]	catalase	+	1.11.1.6
[Ref.: #66714]	cystine arylamidase	-	3.4.11.3
[Ref.: #66714]	cytochrome oxidase	-	1.9.3.1
[Ref.: #66714]	esterase (C 4)	+
[Ref.: #66714]	esterase Lipase (C 8)	+
[Ref.: #66714]	leucine arylamidase	+	3.4.11.1
[Ref.: #66714]	lipase (C 14)	-
[Ref.: #66714]	lysine decarboxylase	+	4.1.1.18
[Ref.: #66714]	N-acetyl-beta-glucosaminidase	-	3.2.1.52
[Ref.: #66714]	naphthol-AS-BI-phosphohydrolase	+
[Ref.: #66714]	ornithine decarboxylase	+	4.1.1.17
[Ref.: #66714]	trypsin	-	3.4.21.4
[Ref.: #66714]	urease	-	3.5.1.5
[Ref.: #66714]	valine arylamidase	-
	Only first 10 entries are displayed. Click here to see all.

Fatty acid profile

Metadata FA analysis

Reference

[Ref.: #66714]

Fatty acid	Percentage
C15:0 anteiso	17.7
C16:0 iso	14.4
C17:0 anteiso	10.1
C16:1 w5c	9.6
C15:1 w5c	7.9
C16:0	7.6
C13:0 anteiso	5.3
C18:0	5.1
C17:0 iso 3OH	3.3
C18:1 w7c/ C18:1 w6c	2.8
C15:0 iso	2.2
C19:0 iso	2.2
C16:1 OH	2.1
C17:0 2OH	1.5
C16:0 iso 3OH	0.9
C19:0 cyclo w8c	0.9

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #66714]	Sample type/isolated from	during bacterial diversity analysis from the subsurface water of Chandra Tal Lake
[Ref.: #66714]	Geographic location (country and/or sea, region)	Chandra Tal Lake in the Spiti valley of the state of Himachal Pradesh
[Ref.: #66714]	Country	India
[Ref.: #66714]	Country ISO 3 Code	IND
[Ref.: #66714]	Continent	Asia
[Ref.: #66714]	Geographic location	32.4349°/77.6375°
[Ref.: #66714]	Enrichment culture	tryptone soya agar
[Ref.: #66714]	Enrichment culture composition	actidione (50 µg ml-1)
[Ref.: #66714]	Enrichment culture duration	15 days
[Ref.: #66714]	Enrichment culture temperature	28 ̊C
[Ref.: #66714]	Isolation/enrichment procedure	isolated during bacterial diversity analysis from the subsurface water of Chandra Tal Lake situated in the cold desert of the Indian trans-Himalayas. Water samples were collected in 2 l sterile Nalgene bottles and stored at 4°C in a refrigerator. The bacterial isolations were made by serial dilution of a 10 ml water sample in 90 ml 0.85 % sterile saline (NaCl 0.85 g l-1), spread plating of 100 µl of a 10-3 dilution on tryptone soya agar (TSA) supplemented with actidione (50 µg ml-1), and incubation at 28 °C for 15 days.

[Ref.: #67770]	Sample type/isolated from	Shallow undersurface water from Chandra Tal Lake
[Ref.: #67770]	Geographic location (country and/or sea, region)	Lahaul-Spiti, Himachal Pradesh
[Ref.: #67770]	Country	India
[Ref.: #67770]	Country ISO 3 Code	IND
[Ref.: #67770]	Continent	Asia
* marker position based on {}

Isolation sources categories

#Environmental

#Aquatic

#Lake (large)

What are isolation sources categories?

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #66714]	Arthrobacter sp. strain IHBB 11108 16S ribosomal RNA gene, partial sequence	KU921532	1493	ENA	1618207 tax ID

Genome sequence information:

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #67770]	Psychromicrobium lacuslunae IHBB 11108	GCA_000950575	complete	GenBank	1618207 tax ID
[Ref.: #66792]	Arthrobacter sp. IHBB 11108	1618207.5	plasmid	PATRIC	1618207 tax ID	*
[Ref.: #66792]	Arthrobacter sp. IHBB 11108	1618207.4	complete	PATRIC	1618207 tax ID	*

[Ref.: #66714]	GC-content	59 mol%	genome sequence analysis
[Ref.: #67770]	GC-content	58.97 mol%	genome sequence analysis

Data predicted using genome information as a basis Genome-based predictions

	Trait	Prediction	Confidence in %	In training data
	Predictions from Diaspora project based on: Arthrobacter sp. IHBB 11108 PATRIC: 1618207.4
[Ref.: #69480]	motile	no	90.86	yes
[Ref.: #69480]	gram-positive	yes	93.213	no
[Ref.: #69480]	anaerobic	no	99.481	no
[Ref.: #69480]	aerobic	yes	93.987	yes
[Ref.: #69480]	halophile	no	90.703	yes
[Ref.: #69480]	spore-forming	no	87.997	no
[Ref.: #69480]	thermophile	no	99.05	yes
[Ref.: #69480]	glucose-util	yes	84.255	no
[Ref.: #69480]	flagellated	no	96.798	yes
[Ref.: #69480]	glucose-ferment	no	89.47	no

Availability in culture collections External links

[Ref.: #66714]

Culture collection no.

JCM 31143, KACC 19070, MCC 2780, MTCC 12460, IHBB 11108

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Psychromicrobium lacuslunae sp. nov., isolated from a high altitude lake.	Kiran S, Schumann P, Busse HJ, Sproer C, Rana A, Pal M, Korpole S, Tewari R, Gulati A	Int J Syst Evol Microbiol	10.1099/ijsem.0.002997	2018	*

References

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#66714

Shashi Kiran, Peter Schumann, Hans-Jürgen Busse, Cathrin Spröer, Aditi Rana, Mohinder Pal, Suresh Korpole, Rupinder Tewari, Arvind Gulati: Psychromicrobium lacuslunae sp. nov., isolated from a high altitude lake. IJSEM 68: 3416 - 3423 2018 ( DOI 10.1099/ijsem.0.002997 , PubMed 30204584 )

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#67770 Japan Collection of Microorganism (JCM) ; Curators of the JCM;

#69480

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

* These data were automatically processed and therefore are not curated

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Search for species Psychromicrobium lacuslunae in external resources:
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Arthrobacter sp. IHBB 11108

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