Name: Brachybacterium endophyticum M1HQ-2
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 159799

Type strain:

Species: Brachybacterium endophyticum

Strain Designation: M1HQ-2

Culture col. no.: KCTC 49087, CGMCC 1.16391, JCM 33403

Strain history: CGMCC 1.16391 <-- L. Tuo; Zunyi Med. Univ., China; M1HQ-2.

NCBI tax ID(s): 2182385 (species)

Section

Brachybacterium endophyticum M1HQ-2 is an aerobe, mesophilic, Gram-positive bacterium that forms circular colonies and was isolated from from a surface-sterilized bark of Scutellaria baicalensis Georgi.

coccus-shaped
colony-forming
Gram-positive
aerobe
mesophilic
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Actinomycetota*
	Class	*Actinomycetes*
	Order	*Micrococcales*
	Family	*Dermabacteraceae*
	Genus	*Brachybacterium*
	Species	**Brachybacterium endophyticum**
	Full Scientific Name (LPSN)	Brachybacterium endophyticum Tuo et al. 2018

Information on morphological and physiological properties Morphology

[Ref.: #66743]	Gram stain	positive

[Ref.: #66743]	Cell length	0.7-1.5 µm

[Ref.: #66743]	Cell shape	coccus-shaped

[Ref.: #66743]	Colony color	yellow
[Ref.: #66743]	Colony shape	circular
[Ref.: #66743]	Cultivation medium used	Marine agar (MA)

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Pigment production

[Ref.: #66743]	Multicellular complex forming ability	no
[Ref.: #66743]	Multicellular complex name	Substrate mycelia and aerial mycelia

Information on culture and growth conditions Culture and growth conditions

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Culture medium

ISP 2

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Culture medium growth

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Culture medium

ISP 3

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Culture medium growth

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Culture medium

ISP 4

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Culture medium growth

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Culture medium

LB (Luria-Bertani) MEDIUM

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Culture medium growth

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Culture medium

Marine agar (MA)

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Culture medium growth

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Culture medium

Nutrient agar (Bacto)

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Culture medium growth

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Culture medium

Reasoner's 2A agar (R2A)

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Culture medium growth

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Culture medium

tryptic soy agar (TSA)

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Culture medium growth

	Kind of temperature		Temperature
[Ref.: #66743]		optimum	30 °C
[Ref.: #66743]	no	growth	42 °C
[Ref.: #66743]		growth	4-37 °C
[Ref.: #67770]		growth	28 °C
[Ref.: #67771]		growth	28 °C

[Ref.: #66743]	Temperature range	mesophilic
[Ref.: #66743]	Temperature range	thermophilic
[Ref.: #67770]	Temperature range	mesophilic
[Ref.: #67771]	Temperature range	mesophilic

	Kind of pH		pH
[Ref.: #66743]		optimum	8
[Ref.: #66743]	no	growth	4
[Ref.: #66743]	no	growth	12
[Ref.: #66743]		growth	5-11

Information on physiology and metabolism Physiology and metabolism

[Ref.: #66743]	Oxygen tolerance	aerobe
[Ref.: #67771]	Oxygen tolerance	aerobe

	Halophily	Salt	Tested relation		Salt conc.
[Ref.: #66743]		NaCl		growth	0-15	%(w/v)
[Ref.: #66743]		NaCl		optimum	1-3	%(w/v)

[Ref.: #66743]	Observation	The cell-wall peptidoglycan contains meso-diaminopimelic acid as the diagnostic diamino acid.
[Ref.: #66743]	Observation	The predominant menaquinone is MK-7.
[Ref.: #66743]	Observation	The polar lipids comprise diphosphatidylglycerol, phosphatidylglycerol, unidentified phospholipid and an unidentified lipid.
[Ref.: #67770]	Observation	quinones: MK-7

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #66743]	3-O-methyl-D-glucose ChEBI	+	oxidation
[Ref.: #66743]	4-hydroxyphenylacetic acid ChEBI	+	oxidation
[Ref.: #66743]	acetic acid ChEBI	+	oxidation
[Ref.: #66743]	acetoacetate ChEBI	+	oxidation
[Ref.: #66743]	adipate ChEBI	-	assimilation
[Ref.: #66743]	alpha-D-glucose ChEBI	+	oxidation
[Ref.: #66743]	alpha-D-glucose 6-phosphate ChEBI	+	oxidation
[Ref.: #66743]	alpha-hydroxybutyrate	+	oxidation
[Ref.: #66743]	bromosuccinate ChEBI	+	oxidation
[Ref.: #66743]	casein	+	hydrolysis
[Ref.: #66743]	cellobiose ChEBI	+	builds acid from
[Ref.: #66743]	cellobiose ChEBI	+	oxidation
[Ref.: #66743]	citric acid ChEBI	+	oxidation
[Ref.: #66743]	D-alanine ChEBI	+	oxidation
[Ref.: #66743]	D-arabinose ChEBI	+	builds acid from
[Ref.: #66743]	D-arabitol ChEBI	+	builds acid from
[Ref.: #66743]	D-arabitol ChEBI	+	oxidation
[Ref.: #66743]	D-aspartate ChEBI	+	oxidation
[Ref.: #66743]	D-fructose ChEBI	+	builds acid from
[Ref.: #66743]	D-fructose ChEBI	+	oxidation
[Ref.: #66743]	D-fructose 6-phosphate ChEBI	+	oxidation
[Ref.: #66743]	D-fucose ChEBI	+	oxidation
[Ref.: #66743]	D-galactonate ChEBI	+	oxidation
[Ref.: #66743]	D-galactonic acid lactone ChEBI	+	oxidation
[Ref.: #66743]	D-galactose ChEBI	+	oxidation
[Ref.: #66743]	D-glucarate ChEBI	+	oxidation
[Ref.: #66743]	D-gluconate ChEBI	+	oxidation
[Ref.: #66743]	D-glucose ChEBI	+	assimilation
[Ref.: #66743]	D-glucose ChEBI	+	builds acid from
[Ref.: #66743]	D-glucuronate ChEBI	+	oxidation
[Ref.: #66743]	D-mannitol ChEBI	+	assimilation
[Ref.: #66743]	D-mannitol ChEBI	+	oxidation
[Ref.: #66743]	D-mannose ChEBI	+	assimilation
[Ref.: #66743]	D-mannose ChEBI	+	builds acid from
[Ref.: #66743]	D-mannose ChEBI	+	oxidation
[Ref.: #66743]	D-serine ChEBI	-	oxidation
[Ref.: #66743]	D-sorbitol ChEBI	+	oxidation
[Ref.: #66743]	D-xylose ChEBI	+	builds acid from
[Ref.: #66743]	decanoate ChEBI	-	assimilation
[Ref.: #66743]	dextrin ChEBI	+	oxidation
[Ref.: #66743]	erythritol ChEBI	+	builds acid from
[Ref.: #66743]	esculin ChEBI	+	builds acid from
[Ref.: #66743]	formate ChEBI	+	oxidation
[Ref.: #66743]	galacturonate ChEBI	+	oxidation
[Ref.: #66743]	gamma-aminobutyric acid ChEBI	+	oxidation
[Ref.: #66743]	gelatin ChEBI	+	hydrolysis
[Ref.: #66743]	gelatin ChEBI	+	oxidation
[Ref.: #66743]	gentiobiose ChEBI	+	oxidation
[Ref.: #66743]	glucose ChEBI	+	fermentation
[Ref.: #66743]	glucuronamide ChEBI	+	oxidation
[Ref.: #66743]	glutaric acid ChEBI	+	oxidation
[Ref.: #66743]	glycerol ChEBI	+	builds acid from
[Ref.: #66743]	glycerol ChEBI	+	oxidation
[Ref.: #66743]	glycine-proline ChEBI	+	oxidation
[Ref.: #66743]	inosine ChEBI	+	oxidation
[Ref.: #66743]	L-arabinose ChEBI	+	assimilation
[Ref.: #66743]	L-arabinose ChEBI	+	builds acid from
[Ref.: #66743]	L-arginine ChEBI	+	oxidation
[Ref.: #66743]	L-aspartate ChEBI	+	oxidation
[Ref.: #66743]	L-fucose ChEBI	-	oxidation
[Ref.: #66743]	L-glutamate ChEBI	+	oxidation
[Ref.: #66743]	L-histidine ChEBI	+	oxidation
[Ref.: #66743]	L-pyroglutamic acid ChEBI	+	oxidation
[Ref.: #66743]	L-rhamnose ChEBI	+	builds acid from
[Ref.: #66743]	L-rhamnose ChEBI	+	oxidation
[Ref.: #66743]	L-serine ChEBI	+	oxidation
[Ref.: #66743]	lactate ChEBI	+	oxidation
[Ref.: #66743]	lactose ChEBI	+	oxidation
[Ref.: #66743]	malate ChEBI	-	assimilation
[Ref.: #66743]	malate ChEBI	+	oxidation
[Ref.: #66743]	maltose ChEBI	+	assimilation
[Ref.: #66743]	maltose ChEBI	+	builds acid from
[Ref.: #66743]	maltose ChEBI	+	oxidation
[Ref.: #66743]	melibiose ChEBI	+	oxidation
[Ref.: #66743]	methyl D-glucoside ChEBI	+	oxidation
[Ref.: #66743]	methyl pyruvate ChEBI	+	oxidation
[Ref.: #66743]	myo-inositol ChEBI	+	builds acid from
[Ref.: #66743]	myo-inositol ChEBI	+	oxidation
[Ref.: #66743]	N-acetyl-beta-D-mannosamine ChEBI	+	oxidation
[Ref.: #66743]	N-acetylgalactosamine ChEBI	+	oxidation
[Ref.: #66743]	N-acetylglucosamine ChEBI	+	assimilation
[Ref.: #66743]	N-acetylglucosamine ChEBI	+	oxidation
[Ref.: #66743]	n-acetylneuraminate ChEBI	+	oxidation
[Ref.: #66743]	pectin ChEBI	+	oxidation
[Ref.: #66743]	phenylacetate ChEBI	-	assimilation
[Ref.: #66743]	potassium gluconate ChEBI	+	assimilation
[Ref.: #66743]	propionate ChEBI	+	oxidation
[Ref.: #66743]	quinate ChEBI	+	oxidation
[Ref.: #66743]	raffinose ChEBI	+	oxidation
[Ref.: #66743]	salicin ChEBI	+	oxidation
[Ref.: #66743]	sodium citrate ChEBI	-	assimilation
[Ref.: #66743]	stachyose ChEBI	+	oxidation
[Ref.: #66743]	starch ChEBI	-	hydrolysis
[Ref.: #66743]	sucrose ChEBI	+	builds acid from
[Ref.: #66743]	sucrose ChEBI	+	oxidation
[Ref.: #66743]	trehalose ChEBI	+	oxidation
[Ref.: #66743]	turanose ChEBI	+	builds acid from
[Ref.: #66743]	turanose ChEBI	+	oxidation
[Ref.: #66743]	tween 20 ChEBI	-	hydrolysis
[Ref.: #66743]	tween 40 ChEBI	+	hydrolysis
[Ref.: #66743]	tween 40 ChEBI	+	oxidation
[Ref.: #66743]	tween 80 ChEBI	-	hydrolysis
	Only first 10 entries are displayed. Click here to see all.

	Metabolite	Antibiotic sensitivity	Antibiotic resistance
[Ref.: #66743]	1 % sodium lactate		yes
[Ref.: #66743]	aztreonam ChEBI		yes
[Ref.: #66743]	fusidate ChEBI	yes
[Ref.: #66743]	guanidinium chloride ChEBI	yes
[Ref.: #66743]	lincomycin ChEBI	yes
[Ref.: #66743]	lithium chloride ChEBI		yes
[Ref.: #66743]	minocycline ChEBI	yes
[Ref.: #66743]	nalidixic acid ChEBI		yes
[Ref.: #66743]	niaproof ChEBI	yes
[Ref.: #66743]	potassium tellurite ChEBI		yes
[Ref.: #66743]	rifamycin sv ChEBI	yes
[Ref.: #66743]	sodium bromate ChEBI		yes
[Ref.: #66743]	sodium butyrate ChEBI		yes
[Ref.: #66743]	tetrazolium blue ChEBI	yes
[Ref.: #66743]	tetrazolium violet ChEBI		yes
[Ref.: #66743]	troleandomycin ChEBI	yes
[Ref.: #66743]	vancomycin ChEBI	yes
	Only first 10 entries are displayed. Click here to see all.

	Metabolite production	Metabolite	Production
[Ref.: #66743]		hydrogen sulfide ChEBI	yes

	Physiological tests	Metabolite	Methylred-test
[Ref.: #66743]		glucose ChEBI	+

	Enzyme	Enzyme activity	EC number
[Ref.: #66743]	acid phosphatase	+	3.1.3.2
[Ref.: #66743]	alkaline phosphatase	+	3.1.3.1
[Ref.: #66743]	alpha-chymotrypsin	-	3.4.21.1
[Ref.: #66743]	alpha-fucosidase	-	3.2.1.51
[Ref.: #66743]	alpha-galactosidase	-	3.2.1.22
[Ref.: #66743]	alpha-glucosidase	+	3.2.1.20
[Ref.: #66743]	beta-galactosidase	+	3.2.1.23
[Ref.: #66743]	beta-glucosidase	+	3.2.1.21
[Ref.: #66743]	beta-glucuronidase	+	3.2.1.31
[Ref.: #66743]	beta-mannosidase	+	3.2.1.25
[Ref.: #66743]	catalase	+	1.11.1.6
[Ref.: #66743]	cystine arylamidase	+	3.4.11.3
[Ref.: #66743]	cytochrome oxidase	-	1.9.3.1
[Ref.: #66743]	esterase (C 4)	+
[Ref.: #66743]	esterase Lipase (C 8)	+
[Ref.: #66743]	leucine arylamidase	+	3.4.11.1
[Ref.: #66743]	lipase (C 14)	-
[Ref.: #66743]	N-acetyl-beta-glucosaminidase	+	3.2.1.52
[Ref.: #66743]	naphthol-AS-BI-phosphohydrolase	+
[Ref.: #66743]	nitrate reductase	-	1.7.99.4
[Ref.: #66743]	trypsin	-	3.4.21.4
[Ref.: #66743]	valine arylamidase	+
	Only first 10 entries are displayed. Click here to see all.

Fatty acid profile

Metadata FA analysis

Reference

[Ref.: #66743]

Fatty acid	Percentage
C15:0 anteiso	69.4
C17:0 anteiso	13.9
C16:0 iso	9.4
C15:0 iso	3
C14:0 iso	2.7
C14:0	1
C16:0	0.6

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #66743]	Sample type/isolated from	from a surface-sterilized bark of Scutellaria baicalensis Georgi
[Ref.: #66743]	Host species	Scutellaria baicalensis
[Ref.: #66743]	Geographic location (country and/or sea, region)	Guizhou
[Ref.: #66743]	Country	China
[Ref.: #66743]	Country ISO 3 Code	CHN
[Ref.: #66743]	Continent	Asia
[Ref.: #66743]	Geographic location	27.3819°/107.7000°
[Ref.: #66743]	Enrichment culture	M1 media
[Ref.: #66743]	Enrichment culture composition	per litre of distilled water: 0.25 g yeast extract, 0.5 g K2HPO4, 20.0 g agar; pH 7.2
[Ref.: #66743]	Enrichment culture duration	2-4 weeks
[Ref.: #66743]	Enrichment culture temperature	28 ̊C
[Ref.: #66743]	Isolation/enrichment procedure	The plant samples were washed and surface-sterilized. After drying in the hood, the surface-sterilized samples were ground into powder by using a micromill and spread onto M1 medium. The isolate was transferred and streaked onto International Streptomyces Project (ISP) 2 agar until pure strains were obtained.

[Ref.: #67770]	Sample type/isolated from	Surface-sterilized bark of Scutellaria baicalensis Georgi from Guizhou
[Ref.: #67770]	Host species	Scutellaria baicalensis
[Ref.: #67770]	Country	China
[Ref.: #67770]	Country ISO 3 Code	CHN
[Ref.: #67770]	Continent	Asia

[Ref.: #67771]	Sample type/isolated from	From medical plant(Scutellaria baicallensis Georgi), forest(N27Â°22'55"E107Â°42'3")
[Ref.: #67771]	Country	China
[Ref.: #67771]	Country ISO 3 Code	CHN
[Ref.: #67771]	Continent	Asia
[Ref.: #67771]	Geographic location	27.3819°/107.7010°
* marker position based on {}

Isolation sources categories

#Host Body-Site	#Plant	#Bark
#Host	#Plants	#Herbaceous plants (Grass,Crops)
#Engineered	#Treatment	#Sterilized (Desinfected)

What are isolation sources categories?

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #66743]	Brachybacterium sp. strain M1HQ-2 16S ribosomal RNA gene, partial sequence	MH289757	1481	ENA	2182385 tax ID

Genome sequence information:

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #67770]	Brachybacterium endophyticum M1HQ-2	GCA_003130585	contig	GenBank	2182385 tax ID
[Ref.: #66792]	Brachybacterium endophyticum M1HQ-2	2837086405	draft	JGI IMG/M	2182385 tax ID	*

[Ref.: #67770]

GC-content

71 mol%

genome sequence analysis

Availability in culture collections External links

[Ref.: #66743]

Culture collection no.

KCTC 49087, CGMCC 1.16391, JCM 33403

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Brachybacterium subflavum sp. nov., a novel actinobacterium isolated from the foregut of grass carp.	Ming H, Cheng LJ, Yi BF, Xia TT, Niu MM, Zhao ZY, Liu BB, Nie GX, Cui CX	Int J Syst Evol Microbiol	10.1099/ijsem.0.004839	2021	*
Phylogeny	Brachybacterium endophyticum sp. nov., a novel endophytic actinobacterium isolated from bark of Scutellaria baicalensis Georgi.	Tuo L, Yan XR, Li FN, Bao YX, Shi HC, Li HY, Sun CH	Int J Syst Evol Microbiol	10.1099/ijsem.0.003032	2018	*

References

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#66743

Li Tuo, Xiao-Rui Yan, Fei-Na Li, Yu-Xin Bao, Hui-Chang Shi, Hong-Ying Li and Cheng-Hang Sun: Brachybacterium endophyticum sp. nov., a novel endophytic actinobacterium isolated from bark of Scutellaria baicalensis Georgi. IJSEM 68: 3563 - 3568 2018 ( DOI 10.1099/ijsem.0.003032 , PubMed 30230442 )

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#67770 Japan Collection of Microorganism (JCM) ; Curators of the JCM;
#67771 Korean Collection for Type Cultures (KCTC) ; Curators of the KCTC;
* These data were automatically processed and therefore are not curated

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