Name: Bradyrhizobium ripae LMG 30283
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 159767

Type strain:

Species: Bradyrhizobium ripae

Culture col. no.: LMG 30283, DSM 105795, NTCCM 0019, WR 4

NCBI tax ID(s): 2364026 (species)

Section

Bradyrhizobium ripae LMG 30283 is an aerobe, mesophilic, Gram-negative bacterium that forms circular colonies and was isolated from nodule of the wild legume Indigofera rautanenii.

colony-forming
Gram-negative
motile
rod-shaped
aerobe
mesophilic
16S sequence
Bacteria

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Pseudomonadota*
	Class	*Alphaproteobacteria*
	Order	*Hyphomicrobiales*
	Family	*Nitrobacteraceae*
	Genus	*Bradyrhizobium*
	Species	**Bradyrhizobium ripae**
	Full Scientific Name (LPSN)	Bradyrhizobium ripae Bünger et al. 2018

Information on morphological and physiological properties Morphology

[Ref.: #66681]	Gram stain	negative

[Ref.: #66681]	Cell length	2-4 µm

[Ref.: #66681]	Cell width	0.6 µm

[Ref.: #66681]	Cell shape	rod-shaped

[Ref.: #66681]	Motility	yes

[Ref.: #66681]	Colony size	2-3 mm
[Ref.: #66681]	Colony color	beige-whitish
[Ref.: #66681]	Colony shape	circular
[Ref.: #66681]	Incubation period	7 days
[Ref.: #66681]	Cultivation medium used	yeast-mannitol agar

Information on culture and growth conditions Culture and growth conditions

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Culture medium

yeast-mannitol agar

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Culture medium growth

	Temperatures	Kind of temperature		Temperature
[Ref.: #66681]			growth	36 °C

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Temperature range

mesophilic

	pH	Kind of pH		pH
[Ref.: #66681]			growth	5-9

Information on physiology and metabolism Physiology and metabolism

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Oxygen tolerance

aerobe

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Ability of spore formation

	Halophily	Salt	Tested relation		Salt conc.
[Ref.: #66681]		NaCl	no	growth	1	%(w/v)

[Ref.: #66681]	Observation	Colonies have a high mucusproduction.
[Ref.: #66681]	Observation	The mean generation time is approximately 6 h in YMB at 28°C.
[Ref.: #66681]	Observation	All strains produce an alkaline reaction on YMA.
[Ref.: #66681]	Observation	Effective nodules are induced on Vigna unguiculata and Indigofera rautanenii.

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #66681]	2-oxobutanoate ChEBI	+	oxidation
[Ref.: #66681]	3-hydroxybutyrate ChEBI	+	oxidation
[Ref.: #66681]	4-hydroxyphenylacetic acid ChEBI	+	oxidation
[Ref.: #66681]	acetic acid ChEBI	+	oxidation
[Ref.: #66681]	alpha-D-glucose ChEBI	+	oxidation
[Ref.: #66681]	cis-aconitate ChEBI	-	oxidation
[Ref.: #66681]	D-alanine ChEBI	+	oxidation
[Ref.: #66681]	D-galactonate ChEBI	+	oxidation
[Ref.: #66681]	D-galactose ChEBI	+	oxidation
[Ref.: #66681]	D-galacturonic acid ChEBI	-	oxidation
[Ref.: #66681]	D-glucarate ChEBI	+	oxidation
[Ref.: #66681]	D-glucosaminic acid ChEBI	+	oxidation
[Ref.: #66681]	D-mannitol ChEBI	+	oxidation
[Ref.: #66681]	D-sorbitol ChEBI	+	oxidation
[Ref.: #66681]	gamma-aminobutyric acid ChEBI	+	oxidation
[Ref.: #66681]	glucuronamide ChEBI	+	oxidation
[Ref.: #66681]	glycerol ChEBI	+	oxidation
[Ref.: #66681]	glycyl-L-glutamate ChEBI	-	oxidation
[Ref.: #66681]	itaconate ChEBI	-	oxidation
[Ref.: #66681]	L-alanine ChEBI	+	oxidation
[Ref.: #66681]	L-alanylglycine ChEBI	-	oxidation
[Ref.: #66681]	L-asparagine ChEBI	-	oxidation
[Ref.: #66681]	L-aspartate ChEBI	-	oxidation
[Ref.: #66681]	L-glutamate ChEBI	-	oxidation
[Ref.: #66681]	L-leucine ChEBI	+	oxidation
[Ref.: #66681]	L-phenylalanine ChEBI	-	oxidation
[Ref.: #66681]	L-proline ChEBI	-	oxidation
[Ref.: #66681]	L-pyroglutamic acid ChEBI	+	oxidation
[Ref.: #66681]	L-rhamnose ChEBI	+	oxidation
[Ref.: #66681]	lactone ChEBI	+	oxidation
[Ref.: #66681]	malonate ChEBI	+	oxidation
[Ref.: #66681]	methyl pyruvate ChEBI	+	oxidation
[Ref.: #66681]	monomethyl succinate ChEBI	+	oxidation
[Ref.: #66681]	phenylethylamine ChEBI	-	oxidation
[Ref.: #66681]	propionate ChEBI	+	oxidation
[Ref.: #66681]	quinate ChEBI	-	oxidation
[Ref.: #66681]	ribitol ChEBI	-	oxidation
[Ref.: #66681]	sebacic acid ChEBI	+	oxidation
[Ref.: #66681]	urocanic acid ChEBI	-	oxidation
	Only first 10 entries are displayed. Click here to see all.

	Metabolite	Antibiotic resistance	Concentration
[Ref.: #66681]	ampicillin ChEBI	yes	50	µg/mL
[Ref.: #66681]	chloramphenicol ChEBI	yes	50	µg/mL
[Ref.: #66681]	erythromycin ChEBI	yes	50	µg/mL
[Ref.: #66681]	kanamycin ChEBI	yes	20	µg/mL
[Ref.: #66681]	streptomycin ChEBI	yes	5	µg/mL

	Metabolite production	Metabolite	Production
[Ref.: #66681]		indole-3-acetic acid ChEBI	no

	Enzymes	Enzyme	Enzyme activity	EC number
[Ref.: #66681]		catalase	+	1.11.1.6
[Ref.: #66681]		cytochrome oxidase	+	1.9.3.1

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #66681]	Sample type/isolated from	nodule of the wild legume Indigofera rautanenii
[Ref.: #66681]	Host species	Indigofera rautanenii
[Ref.: #66681]	Sampling date	2013
[Ref.: #66681]	Geographic location (country and/or sea, region)	Kavango region close to the Okavango river side at Nkwazi Lodge
[Ref.: #66681]	Country	Namibia
[Ref.: #66681]	Country ISO 3 Code	NAM
[Ref.: #66681]	Continent	Africa
[Ref.: #66681]	Geographic location	-17.8653°/19.9083°
[Ref.: #66681]	Enrichment culture	modified arabinose-gluconate medium (MAG) including also yeast extract
[Ref.: #66681]	Isolation/enrichment procedure	The nodules were disinfected, crushed, and rhizobia were isolated as described previously [6]. Pure cultures were grown on modified arabinose-gluconate medium including also yeast extract.
* marker position based on {}

Isolation sources categories

#Host	#Plants	#Herbaceous plants (Grass,Crops)
#Host Body-Site	#Plant	#Root nodule

What are isolation sources categories?

[Ref.: #69479]

Global distribution of 16S sequence MF593081 (>99% sequence identity) for Bradyrhizobium from Microbeatlas

Aquatic Samples	4833
Soil Samples	14338
Animal Samples	6732
Plant Samples	3278
Total Samples	29181

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #66681]	Bradyrhizobium sp. strain WR4 16S ribosomal RNA gene, partial sequence	MF593081	1484	ENA	2364026 tax ID

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GC-content

63.5 mol%

genome sequence analysis

Availability in culture collections External links

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Culture collection no.

LMG 30283, DSM 105795, NTCCM 0019, WR 4

Literature:

	Title	Authors	Journal	DOI	Year
Phylogeny	Bradyrhizobium ripae sp. nov., a nitrogen-fixing symbiont isolated from nodules of wild legumes in Namibia.	Bunger W, Gronemeyer JL, Sarkar A, Reinhold-Hurek B	Int J Syst Evol Microbiol	10.1099/ijsem.0.002955	2018	*
	Root Nodule Rhizobia From Undomesticated Shrubs of the Dry Woodlands of Southern Africa Can Nodulate Angolan Teak Pterocarpus angolensis, an Important Source of Timber.	Bunger W, Sarkar A, Gronemeyer JL, Zielinski J, Revermann R, Hurek T, Reinhold-Hurek B	Front Microbiol	10.3389/fmicb.2021.611704	2021	*

References

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#66681

Wiebke Bünger, Jann Lasse Grönemeyer, Abhijit Sarkar, Barbara Reinhold-Hurek: Bradyrhizobium ripae sp. nov., a nitrogen-fixing symbiont isolated from nodules of wild legumes in Namibia. IJSEM 68: 3688 - 3695 2018 ( DOI 10.1099/ijsem.0.002955 , PubMed 30247121 )

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

* These data were automatically processed and therefore are not curated

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