Name: Acinetobacter wuhouensis WCHA60
Creator: BacDive
License: https://creativecommons.org/licenses/by/4.0/

Strain identifier

BacDive ID: 159758

Type strain:

Species: Acinetobacter wuhouensis

Strain Designation: WCHA60

Culture col. no.: CCTCC AB 2016204, GDMCC 1.1100, KCTC 52505

Strain history: <- Hu Yiyi, West China Hospital of Sichuan Univ.

NCBI tax ID(s): 1879050 (species)

Special Collections

Section

When using BacDive for research please cite our paper

Acinetobacter wuhouensis WCHA60 is an aerobe, Gram-negative, rod-shaped bacterium that forms circular colonies and was isolated from hospital sewage.

colony-forming
Gram-negative
rod-shaped
aerobe
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	09-12-2024 (DD-MM-YYYY)
	Domain	*Bacteria*
	Phylum	*Pseudomonadota*
	Class	*Gammaproteobacteria*
	Order	*Pseudomonadales*
	Family	*Moraxellaceae*
	Genus	*Acinetobacter*
	Species	**Acinetobacter wuhouensis**
	Full Scientific Name (LPSN)	Acinetobacter wuhouensis Hu et al. 2018

Information on morphological and physiological properties Morphology

[Ref.: #66686]	Gram stain	negative
[Ref.: #125438]	Gram stain	negative Confidence in %96.5

[Ref.: #66686]	Cell shape	rod-shaped

[Ref.: #66686]	Type of hemolysis	gamma
[Ref.: #66686]	Colony size	1-1.5 mm
[Ref.: #66686]	Colony color	light yellow
[Ref.: #66686]	Colony shape	circular
[Ref.: #66686]	Incubation period	1 day
[Ref.: #66686]	Cultivation medium used	Tryptic Soy Agar

Information on culture and growth conditions Culture and growth conditions

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Culture medium

BHI agar

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Culture medium growth

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Culture medium

LB (Luria-Bertani) agar

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Culture medium growth

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Culture medium

Mueller-Hinton agar

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Culture medium growth

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Culture medium

tryptic soy agar (TSA)

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Culture medium growth

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Culture medium

tryptic soy broth

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Culture medium growth

	Kind of temperature		Temperature
[Ref.: #66686]	negative	growth	37 °C
[Ref.: #66686]	negative	growth	>37 °C
[Ref.: #66686]		growth	20-35 °C
[Ref.: #67771]		growth	30 °C

	pH	Kind of pH		pH
[Ref.: #66686]			growth	5-10

Information on physiology and metabolism Physiology and metabolism

[Ref.: #66686]	Oxygen tolerance	aerobe
[Ref.: #67771]	Oxygen tolerance	aerobe

	Halophily	Salt	Tested relation		Salt conc.
[Ref.: #66686]		NaCl		growth	0-3	%

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Observation

Cells are incapable of swimming motility.

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #66686]	(R)-malic acid ChEBI	+	growth
[Ref.: #66686]	2,3-butanediol ChEBI	+	carbon source
[Ref.: #66686]	4-hydroxybenzoate ChEBI	-	growth
[Ref.: #66686]	4-oxopentanoate ChEBI	-	growth
[Ref.: #66686]	acetic acid ChEBI	+	carbon source
[Ref.: #66686]	adipate ChEBI	-	growth
[Ref.: #66686]	azelaate ChEBI	-	growth
[Ref.: #66686]	benzoate ChEBI	+	growth
[Ref.: #66686]	beta-alanine ChEBI	-	growth
[Ref.: #66686]	citraconate ChEBI	-	growth
[Ref.: #66686]	citric acid ChEBI	+	carbon source
[Ref.: #66686]	D-gluconate ChEBI	-	growth
[Ref.: #66686]	D-glucose ChEBI	-	builds acid from
[Ref.: #66686]	D-glucose ChEBI	-	growth
[Ref.: #66686]	D-ribose ChEBI	-	growth
[Ref.: #66686]	ethanol ChEBI	+	carbon source
[Ref.: #66686]	gamma-aminobutyric acid ChEBI	-	growth
[Ref.: #66686]	gelatin ChEBI	-	hydrolysis
[Ref.: #66686]	gentisate ChEBI	-	growth
[Ref.: #66686]	glutaric acid ChEBI	+	carbon source
[Ref.: #66686]	histamine ChEBI	-	growth
[Ref.: #66686]	L-arabinose ChEBI	-	growth
[Ref.: #66686]	L-arginine ChEBI	-	growth
[Ref.: #66686]	L-aspartate ChEBI	-	growth
[Ref.: #66686]	L-glutamate ChEBI	+	carbon source
[Ref.: #66686]	L-histidine ChEBI	-	growth
[Ref.: #66686]	L-leucine ChEBI	-	growth
[Ref.: #66686]	L-ornithine ChEBI	-	growth
[Ref.: #66686]	L-phenylalanine ChEBI	-	growth
[Ref.: #66686]	L-tartrate ChEBI	-	growth
[Ref.: #66686]	lactate ChEBI	+	carbon source
[Ref.: #66686]	malonate ChEBI	+	growth
[Ref.: #66686]	phenylacetate ChEBI	+	carbon source
[Ref.: #66686]	putrescine ChEBI	-	growth
[Ref.: #66686]	trans-aconitate ChEBI	-	growth
[Ref.: #66686]	tricarballylate ChEBI	-	growth
[Ref.: #66686]	trigonelline ChEBI	-	growth
[Ref.: #66686]	tryptamine ChEBI	-	growth
	Only first 10 entries are displayed. Click here to see all.

	Enzymes	Enzyme	Enzyme activity	EC number
[Ref.: #66686]		catalase	+	1.11.1.6
[Ref.: #66686]		cytochrome oxidase	-	1.9.3.1

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #66686]	Sample type/isolated from	hospital sewage
[Ref.: #66686]	Sampling date	2015-11
[Ref.: #66686]	Geographic location (country and/or sea, region)	West China Hospital of Sichuan University in Wuhou District, Chengdu
[Ref.: #66686]	Country	China
[Ref.: #66686]	Country ISO 3 Code	CHN
[Ref.: #66686]	Continent	Asia
[Ref.: #66686]	Enrichment culture	nutrient broth
[Ref.: #66686]	Enrichment culture duration	overnight
[Ref.: #66686]	Enrichment culture temperature	30 ̊C
[Ref.: #66686]	Isolation/enrichment procedure	The sewage sample was added to 100 ml nutrient broth (Oxoid) and was incubated over night at 30°C with shaking. The obtained culture suspension was diluted to 1:100 after being adjusted to 0.5 McFarland standard and a 100 µl aliquot was plated onto an Acinetobacter chromogenic agar plate containing 32 µg ml-1 meropenem and 32 µg ml-1 linezolid. Meropenem was added to detect carbapenem-resistant strains and linezolid was added to inhibit the growth of Gram-positive bacteria. The plate were then incubated at 30°C overnight.

[Ref.: #67771]	Sample type/isolated from	From hospital sewage
[Ref.: #67771]	Country	China
[Ref.: #67771]	Country ISO 3 Code	CHN
[Ref.: #67771]	Continent	Asia
* marker position based on {}

Isolation sources categories

#Engineered	#Waste	#Sewage sludge
#Environmental	#Aquatic	-
#Infection	#Medical environment	#Clinic

What are isolation sources categories?

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #66686]	Acinetobacter wuhouensis strain WCHA60 16S ribosomal RNA gene, complete sequence	KY853661	1535	GenBank ENA	1879050 tax ID

Genome sequence information:

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #66792]	Acinetobacter wuhouensis WCHA60	GCA_001696605	complete	GenBank ENA	1879050 tax ID	*
[Ref.: #66792]	Acinetobacter wuhouensis WCHA60	2767802240	draft	JGI IMG/M	1879050 tax ID	*

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GC-content

38.05 mol%

genome sequence analysis

Data predicted using genome information as a basis Genome-based predictions

	Model	Trait	Prediction	Confidence in %	In training data
	Predictions from bacterial phenotypic traits through improved machine learning using high-quality, curated datasets based on: Acinetobacter wuhouensis WCHA60 NCBI: GCA_001696605.3
[Ref.: #125438]	gram-positive	gram-positiveⓘ	no	96.5	no
[Ref.: #125438]	anaerobic	anaerobicⓘ	no	91.065	no
[Ref.: #125438]	aerobic	aerobicⓘ	yes	74.924	yes
[Ref.: #125438]	spore-forming	spore-formingⓘ	no	86.253	no
[Ref.: #125438]	thermophile	thermophilicⓘ	no	96.659	no
[Ref.: #125438]	motile2+	flagellatedⓘ	no	66.604	no

Availability in culture collections External links

[Ref.: #66686]

Culture collection no.

CCTCC AB 2016204, GDMCC 1.1100, KCTC 52505

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Acinetobacter wuhouensis sp. nov., isolated from hospital sewage.	Hu Y, Feng Y, Qin J, Radolfova-Krizova L, Maixnerova M, Zhang X, Nemec A, Zong Z	Int J Syst Evol Microbiol	10.1099/ijsem.0.002963	2018	*

References

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#66686

Yiyi Hu, Yu Feng, Jiayuan Qin, Lenka Radolfova-Krizova, Martina Maixnerova, Xiaoxia Zhang, Alexandr Nemec, Zhiyong Zong: Acinetobacter wuhouensis sp. nov., isolated from hospital sewage. IJSEM 68: 3212 - 3216 2018 ( DOI 10.1099/ijsem.0.002963 , PubMed 30117799 )

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#67771 Korean Collection for Type Cultures (KCTC) ; Curators of the KCTC;

#125438

Julia Koblitz, Lorenz Christian Reimer, Rüdiger Pukall, Jörg Overmann: Predicting bacterial phenotypic traits through improved machine learning using high-quality, curated datasets. 2024 ( DOI 10.1101/2024.08.12.607695 )

* These data were automatically processed and therefore are not curated

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