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Strain identifier

BacDive ID: 159747

Type strain: Yes

Species: Actinomycetospora endophytica

Strain Designation: A-T 8314

Culture col. no.: TBRC 5722, NBRC 113235

NCBI tax ID(s): 2291215 (species)

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Actinomycetospora endophytica A-T 8314 is an aerobe, spore-forming, Gram-positive bacterium that was isolated from roots of orchid .

  1. Gram-positive
  2. spore-forming
  3. aerobe
  4. 16S sequence
  5. Bacteria
  • Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental informationarrow_down
  • [Ref.: #66667] Sample type/isolated from roots of orchid (Podochilus microphyllus Lindl.)
    [Ref.: #66667] Host species Podochilus microphyllus
    [Ref.: #66667] Geographic location (country and/or sea, region) Trat Province
    [Ref.: #66667] Country Thailand
    [Ref.: #66667] Country ISO 3 Code THA
    [Ref.: #66667] Continent Asia
    [Ref.: #66667] Geographic location 12.6183°/102.5770°
    [Ref.: #66667] Enrichment culture humic acid-vitamin agar
    [Ref.: #66667] Enrichment culture composition supplemented with 50 mg l-1 of nalidixic acid, 100 mg l-1 of cycloheximide and 2 mg l-1 of terbinafine
    [Ref.: #66667] Enrichment culture duration 21 days
    [Ref.: #66667] Enrichment culture temperature 28  ̊C
    [Ref.: #66667] Isolation/enrichment procedure The roots were surface sterilized as follows: sequential immersion in 70 % (v/v) ethanol for 5 min and sodium hypochlorite solution (4 % available chloride, freshly prepared) for 5 min. Then the sample was washed three times with sterile distilled water to remove surface sterilization agents and soaked in 10 % NaHCO3 solution for 10 min to eliminate growth of endophytic fungi. One gram of the sample was cut into small pieces with sterilized scissors and then crushed with mortar and pestle in 9 ml sterile sodium dodecyl sulfate (SDS) solution (0.1 g SDS, 1.75 g KH2PO4, 3.5 g K2HPO4, 1 l distilled water), 10-fold serially diluted in SDS solution and plated on humic acid-vitamin agar and incubated at 28°C for 21 days.
    * marker position based on {}
    Isolation sources categories
    #Host #Plants #Herbaceous plants (Grass,Crops)
    #Host Body-Site #Plant #Root (Rhizome)

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