Name: Nocardioides currus IB-3
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 159744

Type strain:

Species: Nocardioides currus

Strain Designation: IB-3

Culture col. no.: KACC 19522, JCM 32672

Strain history: C. O. Jeon; Chung-Ang Univ., South Korea; IB-3.

NCBI tax ID(s): 2133958 (species)

Section

Nocardioides currus IB-3 is an aerobe, mesophilic, Gram-positive bacterium that forms circular colonies and was isolated from automobile air-conditioning system.

colony-forming
Gram-positive
rod-shaped
aerobe
mesophilic
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Actinomycetota*
	Class	*Actinomycetes*
	Order	*Propionibacteriales*
	Family	*Nocardioidaceae*
	Genus	*Nocardioides*
	Species	**Nocardioides currus**
	Full Scientific Name (LPSN)	Nocardioides currus Park et al. 2018

Information on morphological and physiological properties Morphology

[Ref.: #66662]	Gram stain	positive

[Ref.: #66662]	Cell length	1.1-1.3 µm

[Ref.: #66662]	Cell width	0.5-0.7 µm

[Ref.: #66662]	Cell shape	rod-shaped

[Ref.: #66662]	Motility	no

[Ref.: #66662]	Colony color	ivory
[Ref.: #66662]	Colony shape	circular
[Ref.: #66662]	Cultivation medium used	R2A medium

Information on culture and growth conditions Culture and growth conditions

[Ref.: #66662]

Culture medium

LB (Luria-Bertani) agar

[Ref.: #66662]

Culture medium growth

[Ref.: #66662]

Culture medium

Nutrient agar (NA)

[Ref.: #66662]

Culture medium growth

[Ref.: #66662]

Culture medium

Reasoner's 2A agar (R2A)

[Ref.: #66662]

Culture medium growth

[Ref.: #66662]

Culture medium

tryptic soy agar (TSA)

[Ref.: #66662]

Culture medium growth

		Temperature
[Ref.: #66662]	optimum	25 °C
[Ref.: #66662]	growth	20-40 °C
[Ref.: #67770]	growth	28 °C

[Ref.: #66662]	Temperature range	mesophilic
[Ref.: #67770]	Temperature range	mesophilic

	pH	Kind of pH		pH
[Ref.: #66662]			optimum	7.5
[Ref.: #66662]			growth	6.5-9

Information on physiology and metabolism Physiology and metabolism

[Ref.: #66662]

Oxygen tolerance

aerobe

	Halophily	Salt	Tested relation		Salt conc.
[Ref.: #66662]		NaCl		growth	0-1	%(w/v)
[Ref.: #66662]		NaCl		optimum	0	%(w/v)

[Ref.: #67770]

Observation

quinones: MK-8 (H₄)

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #66662]	adipate ChEBI	-	assimilation
[Ref.: #66662]	casein	+	hydrolysis
[Ref.: #66662]	D-glucose ChEBI	+	assimilation
[Ref.: #66662]	D-mannitol ChEBI	-	assimilation
[Ref.: #66662]	D-mannose ChEBI	+	assimilation
[Ref.: #66662]	decanoate ChEBI	+	assimilation
[Ref.: #66662]	esculin ChEBI	-	hydrolysis
[Ref.: #66662]	L-arabinose ChEBI	+	assimilation
[Ref.: #66662]	malate ChEBI	-	assimilation
[Ref.: #66662]	maltose ChEBI	+	assimilation
[Ref.: #66662]	N-acetylglucosamine ChEBI	+	assimilation
[Ref.: #66662]	nitrate ChEBI	+	reduction
[Ref.: #66662]	phenylacetate ChEBI	-	assimilation
[Ref.: #66662]	potassium gluconate ChEBI	-	assimilation
[Ref.: #66662]	sodium citrate ChEBI	-	assimilation
[Ref.: #66662]	starch ChEBI	-	hydrolysis
[Ref.: #66662]	tween 20 ChEBI	-	hydrolysis
[Ref.: #66662]	tween 80 ChEBI	-	hydrolysis
[Ref.: #66662]	tyrosine ChEBI	-	hydrolysis
	Only first 10 entries are displayed. Click here to see all.

	Enzyme	Enzyme activity	EC number
[Ref.: #66662]	acid phosphatase	+	3.1.3.2
[Ref.: #66662]	alkaline phosphatase	+	3.1.3.1
[Ref.: #66662]	alpha-chymotrypsin	+	3.4.21.1
[Ref.: #66662]	alpha-fucosidase	-	3.2.1.51
[Ref.: #66662]	alpha-galactosidase	+	3.2.1.22
[Ref.: #66662]	alpha-glucosidase	+	3.2.1.20
[Ref.: #66662]	alpha-mannosidase	-	3.2.1.24
[Ref.: #66662]	beta-galactosidase	+	3.2.1.23
[Ref.: #66662]	beta-glucosidase	-	3.2.1.21
[Ref.: #66662]	beta-glucuronidase	-	3.2.1.31
[Ref.: #66662]	catalase	+	1.11.1.6
[Ref.: #66662]	cystine arylamidase	+	3.4.11.3
[Ref.: #66662]	cytochrome oxidase	+	1.9.3.1
[Ref.: #66662]	esterase (C 4)	-
[Ref.: #66662]	esterase Lipase (C 8)	-
[Ref.: #66662]	leucine arylamidase	+	3.4.11.1
[Ref.: #66662]	lipase (C 14)	+
[Ref.: #66662]	N-acetyl-beta-glucosaminidase	-	3.2.1.52
[Ref.: #66662]	naphthol-AS-BI-phosphohydrolase	-
[Ref.: #66662]	trypsin	+	3.4.21.4
[Ref.: #66662]	valine arylamidase	+
	Only first 10 entries are displayed. Click here to see all.

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #66662]	Sample type/isolated from	automobile air-conditioning system
[Ref.: #66662]	Country	Republic of Korea
[Ref.: #66662]	Country ISO 3 Code	KOR
[Ref.: #66662]	Continent	Asia
[Ref.: #66662]	Enrichment culture	R2A agar
[Ref.: #66662]	Enrichment culture duration	7 days
[Ref.: #66662]	Enrichment culture temperature	25 ̊C
[Ref.: #66662]	Isolation/enrichment procedure	heat exchanger aluminium fins were collected from an air-conditioning system of a motor car that was inoperation in South Korea. The aluminium fins were strongly mixed with phosphate-buffered saline (PBS; 137 mM NaCl, 2.7 mM KCl, 10 mM Na2HPO4 , 2 mM KH2PO4, pH 7.2) for 10 min and then the resuspended solution was serially diluted with PBS. The aliquots of each serial dilution were spread on R2A agar (BD) and incubated aerobically at 25°C for 7 days.

[Ref.: #67770]	Sample type/isolated from	Automobile air-conditioning system
[Ref.: #67770]	Country	Republic of Korea
[Ref.: #67770]	Country ISO 3 Code	KOR
[Ref.: #67770]	Continent	Asia
* marker position based on {}

Isolation sources categories

#Engineered

#Built environment

#Air conditioner

What are isolation sources categories?

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #66662]	Nocardioides sp. strain IB-3 16S ribosomal RNA gene, partial sequence	MG745377	1438	ENA	2133958 tax ID

Genome sequence information:

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #67770]	Nocardioides currus IB-3	GCA_003057875	contig	GenBank	2133958 tax ID
[Ref.: #66792]	Nocardioides currus IB-3	2834618132	draft	JGI IMG/M	2133958 tax ID	*

[Ref.: #67770]

GC-content

71.5 mol%

genome sequence analysis

Availability in culture collections External links

[Ref.: #66662]

Culture collection no.

KACC 19522, JCM 32672

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Nocardioides euryhalodurans sp. nov., Nocardioides seonyuensis sp. nov. and Nocardioides eburneiflavus sp. nov., isolated from soil.	Roh SG, Lee C, Kim MK, Kang HJ, Kim YS, Kim MJ, Malik A, Kim SB	Int J Syst Evol Microbiol	10.1099/ijsem.0.004095	2020	*
Phylogeny	Nocardioides currus sp. nov., isolated from a mobile car air-conditioning system.	Park YJ, Jeong SE, Jung HS, Park SY, Jeon CO	Int J Syst Evol Microbiol	10.1099/ijsem.0.002933	2018	*
Phylogeny	Nocardioides nematodiphilus sp. nov., isolated from rhizosphere of Arabidopsis thaliana.	Zhang XM, Mo WT, Wei YQ, Li X, Ma GM, Yang YS, Wang XJ, Liu JJ, Liu ZY, Zhou XK, Zhang TK	Int J Syst Evol Microbiol	10.1099/ijsem.0.005271	2022	*

References

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#66662

Yu Jin Park, Sang Eun Jeong, Hye Su Jung, So Yoon Park, Che Ok Jeon: Nocardioides currus sp. nov., isolated from a mobile car air-conditioning system. IJSEM 68: 2977 - 2982 2018 ( DOI 10.1099/ijsem.0.002933 , PubMed 30028286 )

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#67770 Japan Collection of Microorganism (JCM) ; Curators of the JCM;
* These data were automatically processed and therefore are not curated

Change proposal

You found an error in BacDive? Please tell us about it!

Note that changes will be reviewed and judged. If your changes are legitimate, changes will occur within the next BacDive update. Only proposed changes supported by the according reference will be reviewed. The BacDive team reserves the right to reject proposed changes.

Search for species Nocardioides currus in external resources:
SILVA
BRENDA
PANGAEA
StrainInfo
GBIF