A tetrabro-mobisphenol A (TBBPA)-contaminated soil sample (5.0 g) was added to a 250 ml flask containing 100 ml minimal medium (1.0 g NH4NO3, 1.6 g K2HPO4, 0.5 g KH2PO4, 0.2 g MgSO4 and 1.0 g NaCl per litre water at pH 7.0) supplemented with 0.1 mM TBBPA (TMM). The culture was incubated at 30°C at 150 r.p.m. for 5 days. A 5 ml aliquot of the enriched culture was subsequently transferred into 100 ml of fresh TMM. After three rounds of enrichment, the mixture was diluted in a tenfold series, and a 100 µl sample of each dilution was spread onto trypticase soy agar plates. The plates were incubated at 30°C for 3 days, and then all colonies were isolated.
Korean Collection for Type Cultures (KCTC) ; Curators of the KCTC;
#69479
João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.:
MicrobeAtlas 1.0 beta
.
* These data were automatically processed and therefore are not curated
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