Name: Sphingomonas tabacisoli X1-8
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 159719

Type strain:

Species: Sphingomonas tabacisoli

Strain Designation: X1-8

Culture col. no.: KCTC 62032, CGMCC 1.16275

Strain history: <- Wenjun Li, Sun Sat-Sen Univ.

NCBI tax ID(s): 2249466 (species)

Section

Sphingomonas tabacisoli X1-8 is an aerobe, psychrophilic, Gram-negative bacterium that forms circular colonies and was isolated from rhizosphere soil of Nicotiana tabacum L., collected from a tobacco produce base.

colony-forming
Gram-negative
motile
rod-shaped
aerobe
psychrophilic
16S sequence
Bacteria

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Pseudomonadota*
	Class	*Alphaproteobacteria*
	Order	*Sphingomonadales*
	Family	*Sphingomonadaceae*
	Genus	*Sphingomonas*
	Species	**Sphingomonas tabacisoli**
	Full Scientific Name (LPSN)	Sphingomonas tabacisoli Zhou et al. 2018

Information on morphological and physiological properties Morphology

[Ref.: #66619]	Gram stain	negative

[Ref.: #66619]	Cell length	0.7-1.6 µm

[Ref.: #66619]	Cell width	0.4-0.5 µm

[Ref.: #66619]	Cell shape	rod-shaped

[Ref.: #66619]	Motility	yes

[Ref.: #66619]	Flagellum arrangement	peritrichous

[Ref.: #66619]	Colony size	2-3 mm
[Ref.: #66619]	Colony color	yellow
[Ref.: #66619]	Colony shape	circular
[Ref.: #66619]	Incubation period	7 days
[Ref.: #66619]	Cultivation medium used	R2A medium

Information on culture and growth conditions Culture and growth conditions

[Ref.: #66619]

Culture medium

Reasoner's 2A agar (R2A)

[Ref.: #66619]

Culture medium growth

	Kind of temperature		Temperature
[Ref.: #66619]	no	growth	20 °C
[Ref.: #66619]	no	growth	45 °C
[Ref.: #66619]		growth	25-40 °C
[Ref.: #66619]		optimum	30-35 °C
[Ref.: #67771]		growth	30 °C

[Ref.: #66619]	Temperature range	psychrophilic
[Ref.: #66619]	Temperature range	thermophilic
[Ref.: #66619]	Temperature range	mesophilic
[Ref.: #67771]	Temperature range	mesophilic

	Kind of pH		pH
[Ref.: #66619]		optimum	7
[Ref.: #66619]	no	growth	5
[Ref.: #66619]	no	growth	9
[Ref.: #66619]		growth	6-8

Information on physiology and metabolism Physiology and metabolism

[Ref.: #66619]	Oxygen tolerance	aerobe
[Ref.: #67771]	Oxygen tolerance	aerobe

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #66619]	(R)-malic acid ChEBI	-	carbon source
[Ref.: #66619]	(S)-lactate ChEBI	-	carbon source
[Ref.: #66619]	2-hydroxybutyrate ChEBI	+	carbon source
[Ref.: #66619]	2-oxobutanoate ChEBI	-	carbon source
[Ref.: #66619]	2-oxoglutarate ChEBI	-	carbon source
[Ref.: #66619]	3-O-methyl-D-glucose ChEBI	-	carbon source
[Ref.: #66619]	4-hydroxyphenylacetic acid ChEBI	-	carbon source
[Ref.: #66619]	acetoacetate ChEBI	+	carbon source
[Ref.: #66619]	alpha-D-glucose ChEBI	+	carbon source
[Ref.: #66619]	amygdalin ChEBI	+	builds acid from
[Ref.: #66619]	amygdalin ChEBI	-	carbon source
[Ref.: #66619]	arbutin ChEBI	+	builds acid from
[Ref.: #66619]	bromosuccinate ChEBI	-	carbon source
[Ref.: #66619]	casein	-	hydrolysis
[Ref.: #66619]	cellobiose ChEBI	+	builds acid from
[Ref.: #66619]	cellobiose ChEBI	-	carbon source
[Ref.: #66619]	cellulose ChEBI	-	hydrolysis
[Ref.: #66619]	citric acid ChEBI	-	assimilation
[Ref.: #66619]	D-arabinose ChEBI	-	builds acid from
[Ref.: #66619]	D-arabitol ChEBI	-	builds acid from
[Ref.: #66619]	D-fructose ChEBI	-	builds acid from
[Ref.: #66619]	D-fructose ChEBI	+	carbon source
[Ref.: #66619]	D-fructose 6-phosphate ChEBI	-	carbon source
[Ref.: #66619]	D-fucose ChEBI	-	builds acid from
[Ref.: #66619]	D-galactose ChEBI	-	builds acid from
[Ref.: #66619]	D-galactose ChEBI	+	carbon source
[Ref.: #66619]	D-glucose ChEBI	-	builds acid from
[Ref.: #66619]	D-glucuronate ChEBI	+	carbon source
[Ref.: #66619]	D-lyxose ChEBI	-	builds acid from
[Ref.: #66619]	D-mannitol ChEBI	-	builds acid from
[Ref.: #66619]	D-mannose ChEBI	-	builds acid from
[Ref.: #66619]	D-mannose ChEBI	+	carbon source
[Ref.: #66619]	D-ribose ChEBI	-	builds acid from
[Ref.: #66619]	D-serine ChEBI	-	carbon source
[Ref.: #66619]	D-serine ChEBI	-	nitrogen source
[Ref.: #66619]	D-sorbitol ChEBI	-	builds acid from
[Ref.: #66619]	D-sorbitol ChEBI	-	carbon source
[Ref.: #66619]	D-tagatose ChEBI	-	builds acid from
[Ref.: #66619]	D-xylose ChEBI	-	builds acid from
[Ref.: #66619]	dextrin ChEBI	+	carbon source
[Ref.: #66619]	erythritol ChEBI	-	builds acid from
[Ref.: #66619]	esculin ferric citrate	+	builds acid from
[Ref.: #66619]	galactitol ChEBI	-	builds acid from
[Ref.: #66619]	gamma-aminobutyric acid ChEBI	-	carbon source
[Ref.: #66619]	gamma-aminobutyric acid ChEBI	-	nitrogen source
[Ref.: #66619]	gelatin ChEBI	-	hydrolysis
[Ref.: #66619]	gentiobiose ChEBI	-	builds acid from
[Ref.: #66619]	gentiobiose ChEBI	+	carbon source
[Ref.: #66619]	glycerol ChEBI	-	builds acid from
[Ref.: #66619]	glycogen ChEBI	-	builds acid from
[Ref.: #66619]	inosine ChEBI	-	carbon source
[Ref.: #66619]	inulin ChEBI	+	builds acid from
[Ref.: #66619]	L-arabinose ChEBI	-	builds acid from
[Ref.: #66619]	L-arabitol ChEBI	-	builds acid from
[Ref.: #66619]	L-aspartate ChEBI	+	carbon source
[Ref.: #66619]	L-aspartate ChEBI	+	nitrogen source
[Ref.: #66619]	L-fucose ChEBI	-	builds acid from
[Ref.: #66619]	L-glutamate ChEBI	-	nitrogen source
[Ref.: #66619]	L-histidine ChEBI	-	carbon source
[Ref.: #66619]	L-histidine ChEBI	-	nitrogen source
[Ref.: #66619]	L-rhamnose ChEBI	+	builds acid from
[Ref.: #66619]	L-rhamnose ChEBI	+	carbon source
[Ref.: #66619]	L-serine ChEBI	-	carbon source
[Ref.: #66619]	L-serine ChEBI	-	nitrogen source
[Ref.: #66619]	L-sorbose ChEBI	-	builds acid from
[Ref.: #66619]	L-xylose ChEBI	-	builds acid from
[Ref.: #66619]	lactose ChEBI	-	builds acid from
[Ref.: #66619]	lactose ChEBI	-	carbon source
[Ref.: #66619]	maltose ChEBI	-	builds acid from
[Ref.: #66619]	maltose ChEBI	-	carbon source
[Ref.: #66619]	melezitose ChEBI	-	builds acid from
[Ref.: #66619]	melibiose ChEBI	-	builds acid from
[Ref.: #66619]	methyl alpha-D-glucopyranoside ChEBI	-	builds acid from
[Ref.: #66619]	methyl alpha-D-mannoside ChEBI	-	builds acid from
[Ref.: #66619]	methyl beta-D-xylopyranoside ChEBI	-	builds acid from
[Ref.: #66619]	methyl pyruvate ChEBI	+	carbon source
[Ref.: #66619]	myo-inositol ChEBI	-	builds acid from
[Ref.: #66619]	N-acetylglucosamine ChEBI	-	builds acid from
[Ref.: #66619]	n-acetylneuraminate ChEBI	+	carbon source
[Ref.: #66619]	nitrate ChEBI	-	reduction
[Ref.: #66619]	pectin ChEBI	+	carbon source
[Ref.: #66619]	potassium 2-dehydro-D-gluconate	-	builds acid from
[Ref.: #66619]	potassium 5-dehydro-D-gluconate	-	builds acid from
[Ref.: #66619]	potassium gluconate ChEBI	-	builds acid from
[Ref.: #66619]	propionate ChEBI	-	carbon source
[Ref.: #66619]	raffinose ChEBI	-	builds acid from
[Ref.: #66619]	raffinose ChEBI	-	carbon source
[Ref.: #66619]	ribitol ChEBI	-	builds acid from
[Ref.: #66619]	salicin ChEBI	-	builds acid from
[Ref.: #66619]	salicin ChEBI	+	carbon source
[Ref.: #66619]	stachyose ChEBI	+	carbon source
[Ref.: #66619]	starch ChEBI	-	builds acid from
[Ref.: #66619]	starch ChEBI	-	hydrolysis
[Ref.: #66619]	sucrose ChEBI	+	builds acid from
[Ref.: #66619]	sucrose ChEBI	+	carbon source
[Ref.: #66619]	trehalose ChEBI	+	builds acid from
[Ref.: #66619]	tryptophan ChEBI	-	energy source
[Ref.: #66619]	turanose ChEBI	-	builds acid from
[Ref.: #66619]	turanose ChEBI	-	carbon source
[Ref.: #66619]	tween 20 ChEBI	+	hydrolysis
[Ref.: #66619]	tween 40 ChEBI	+	hydrolysis
[Ref.: #66619]	tween 60 ChEBI	+	hydrolysis
[Ref.: #66619]	tween 80 ChEBI	-	hydrolysis
[Ref.: #66619]	xylitol ChEBI	-	builds acid from
	Only first 10 entries are displayed. Click here to see all.

	Metabolite	Production
[Ref.: #66619]	acetoin ChEBI	yes
[Ref.: #66619]	hydrogen sulfide ChEBI	no
[Ref.: #66619]	indole ChEBI	no

	Physiological tests	Metabolite	Voges-Proskauer-test	Indole test
[Ref.: #66619]		acetoin ChEBI	+
[Ref.: #66619]		indole ChEBI		-

	Enzyme	Enzyme activity	EC number
[Ref.: #66619]	acid phosphatase	+	3.1.3.2
[Ref.: #66619]	alkaline phosphatase	+	3.1.3.1
[Ref.: #66619]	alpha-galactosidase	+	3.2.1.22
[Ref.: #66619]	alpha-glucosidase	+	3.2.1.20
[Ref.: #66619]	alpha-mannosidase	-	3.2.1.24
[Ref.: #66619]	arginine dihydrolase	-	3.5.3.6
[Ref.: #66619]	beta-D-fucosidase	-	3.2.1.38
[Ref.: #66619]	beta-galactosidase	+	3.2.1.23
[Ref.: #66619]	beta-glucosidase	+	3.2.1.21
[Ref.: #66619]	beta-glucuronidase	+	3.2.1.31
[Ref.: #66619]	catalase	+	1.11.1.6
[Ref.: #66619]	chymotrypsin	+	3.4.4.5
[Ref.: #66619]	cystine arylamidase	+	3.4.11.3
[Ref.: #66619]	cytochrome oxidase	-	1.9.3.1
[Ref.: #66619]	esterase (C 4)	+
[Ref.: #66619]	esterase Lipase (C 8)	+
[Ref.: #66619]	leucine arylamidase	+	3.4.11.1
[Ref.: #66619]	lipase (C 14)	-
[Ref.: #66619]	lysine decarboxylase	-	4.1.1.18
[Ref.: #66619]	N-acetyl-beta-glucosaminidase	+	3.2.1.52
[Ref.: #66619]	naphthol-AS-BI-phosphohydrolase	+
[Ref.: #66619]	ornithine decarboxylase	-	4.1.1.17
[Ref.: #66619]	trypsin	+	3.4.21.4
[Ref.: #66619]	tryptophan deaminase	-	4.1.99.1
[Ref.: #66619]	urease	-	3.5.1.5
[Ref.: #66619]	valine arylamidase	+
	Only first 10 entries are displayed. Click here to see all.

Fatty acid profile

Metadata FA analysis

Reference

[Ref.: #66619]

Fatty acid	Percentage
C18:1 w7c and/or C18:1 w6c	60.1
C16:0	18.5
C17:1 w6c	7.9
11 methyl- C18:1w7c	2.8
C14:0 2OH	2.7
C16:1 w7c and/or C16:1 w6c	2.5
C16:1 w5c	1.2
C18:1 w5c	1.1
C16:0 2OH	1
C18:0	0.5
C17:0	0.5

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #66619]	Sample type/isolated from	rhizosphere soil of Nicotiana tabacum L., collected from a tobacco produce base
[Ref.: #66619]	Host species	Nicotiana tabacum
[Ref.: #66619]	Geographic location (country and/or sea, region)	Kunming
[Ref.: #66619]	Country	China
[Ref.: #66619]	Country ISO 3 Code	CHN
[Ref.: #66619]	Continent	Asia
[Ref.: #66619]	Enrichment culture	R2A agar
[Ref.: #66619]	Enrichment culture duration	2 weeks
[Ref.: #66619]	Enrichment culture temperature	30 ̊C
[Ref.: #66619]	Isolation/enrichment procedure	The soil sample for this study was diluted to concentrations of 10-3 and 10-4 (w/v), then 0.1 ml of each dilution was spread on R2A agar plates. Colonies of X1-8T were picked after incubation for two weeks at 30°C.

[Ref.: #67771]	Sample type/isolated from	From soil
[Ref.: #67771]	Geographic location (country and/or sea, region)	Yunnan province
[Ref.: #67771]	Country	China
[Ref.: #67771]	Country ISO 3 Code	CHN
[Ref.: #67771]	Continent	Asia
* marker position based on {}

Isolation sources categories

#Host	#Plants	#Herbaceous plants (Grass,Crops)
#Engineered	#Agriculture	#Plantation
#Host Body-Site	#Plant	#Rhizosphere
#Environmental	#Terrestrial	#Soil

What are isolation sources categories?

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #66619]	Sphingomonas sp. strain X1-8 16S ribosomal RNA gene, partial sequence	MF370621	1446	ENA	2249466 tax ID

[Ref.: #66619]

GC-content

66.5 mol%

high performance liquid chromatography (HPLC)

Availability in culture collections External links

[Ref.: #66619]

Culture collection no.

KCTC 62032, CGMCC 1.16275

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Sphingomonas changnyeongensis sp. nov. isolated from the Hapcheon-Changnyeong barrage area in the Nakdong river.	Park C, Kim M, Lee BH, Lee KE, Park W	Int J Syst Evol Microbiol	10.1099/ijsem.0.004503	2020	*
Phylogeny	Sphingomonas horti sp. nov., a novel bacterial species isolated from soil of a tomato garden.	Akter S, Lee SY, Moon SK, Choi C, Balusamy SR, Siddiqi MZ, Ashrafudoulla M, Huq MA	Arch Microbiol	10.1007/s00203-020-02056-x	2020	*
Phylogeny	Sphingomonas tabacisoli sp. nov., a member of the genus Sphingomonas, isolated from rhizosphere soil of Nicotiana tabacum L.	Zhou XK, Mi QL, Yao JH, Wu H, Liu XM, Li YD, Duan YQ, Chen JH, Dang LZ, Mo MH, Li XM, Li WJ	Int J Syst Evol Microbiol	10.1099/ijsem.0.002879	2018	*

References

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#66619

Xing-Kui Zhou, Qi-Li Mi, Jian-Hua Yao, Heng Wu, Xiu-Ming Liu, Yuan-Dong Li, Yan-Qing Duan, Jian-Hua Chen, Li-Zhi Dang, Ming-He Mo, Xue-Mei Li, Wen-Jun Li: Sphingomonas tabacisoli sp. nov., a member of the genus Sphingomonas, isolated from rhizosphere soil of Nicotiana tabacum L.. IJSEM 68: 2574 - 2579 2018 ( DOI 10.1099/ijsem.0.002879 , PubMed 29944093 )

#67771 Korean Collection for Type Cultures (KCTC) ; Curators of the KCTC;
* These data were automatically processed and therefore are not curated

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