Name: Solimicrobium silvestre S20-91
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 159715

Type strain:

Species: Solimicrobium silvestre

Strain Designation: S20-91

Culture col. no.: DSM 104733, LMG 30010

Strain history: <- R. Margesin, Inst. Microbiology, Univ. Innsbruck, Austria; S20-91

NCBI tax ID(s): 2099400 (species)

SI-ID 407522

DSM 104733

Section

Solimicrobium silvestre S20-91 is an aerobe, mesophilic, Gram-negative bacterium that forms circular colonies and was isolated from alpine forest soil .

colony-forming
Gram-negative
motile
rod-shaped
aerobe
mesophilic
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Pseudomonadota*
	Class	*Betaproteobacteria*
	Order	*Burkholderiales*
	Family	*Oxalobacteraceae*
	Genus	*Solimicrobium*
	Species	**Solimicrobium silvestre**
	Full Scientific Name (LPSN)	Solimicrobium silvestre Margesin et al. 2018

Information on morphological and physiological properties Morphology

[Ref.: #66606]	Gram stain	negative

[Ref.: #66606]	Cell length	1.7-2 µm

[Ref.: #66606]	Cell width	0.8 µm

[Ref.: #66606]	Cell shape	rod-shaped

[Ref.: #66606]	Motility	yes

[Ref.: #66606]	Colony color	light yellow
[Ref.: #66606]	Colony shape	circular
[Ref.: #66606]	Cultivation medium used	Reasoner's 2A agar (R2A)

[Ref.: #66606]	Colony color	creamy
[Ref.: #66606]	Cultivation medium used	modified M162 agar

Multimedia content

[Ref.: #69031]

Caption:

DSM 104733 on M. 830 at 25 °C

[Ref.: #69031]

Intellectual property rights:

Information on culture and growth conditions Culture and growth conditions

[Ref.: #69031]

Culture medium

R2A MEDIUM (DSMZ Medium 830)

[Ref.: #69031]

Culture medium growth

[Ref.: #69031]

Culture medium link

Medium recipe provided by DSMZ

[Ref.: #66606]

Culture medium

modified M162 agar

[Ref.: #66606]

Culture medium growth

[Ref.: #66606]

Culture medium composition

expand / minimize

[Ref.: #66606]

Culture medium

Nutrient agar (NA)

[Ref.: #66606]

Culture medium growth

[Ref.: #66606]

Culture medium composition

expand / minimize

[Ref.: #66606]

Culture medium

Reasoner's 2A agar (R2A)

[Ref.: #66606]

Culture medium growth

[Ref.: #66606]

Culture medium

R2A broth

[Ref.: #66606]

Culture medium growth

[Ref.: #66606]

Culture medium

tryptic soy agar (TSA)

[Ref.: #66606]

Culture medium growth

	Kind of temperature		Temperature
[Ref.: #66606]	no	growth	30 °C
[Ref.: #66606]		growth	0-25 °C
[Ref.: #66606]		optimum	15-25 °C
[Ref.: #69031]		growth	25 °C
[Ref.: #69031]		growth	28 °C

[Ref.: #66606]	Temperature range	mesophilic
[Ref.: #69031]	Temperature range	mesophilic

	pH	Kind of pH		pH
[Ref.: #66606]			optimum	7
[Ref.: #66606]			growth	6-9

Information on physiology and metabolism Physiology and metabolism

[Ref.: #66606]	Oxygen tolerance	aerobe
[Ref.: #66606]	Oxygen tolerance	microaerophile

[Ref.: #66606]

Ability of spore formation

	Halophily	Salt	Tested relation		Salt conc.
[Ref.: #66606]		NaCl		growth	0-1	%(w/v)
[Ref.: #66606]		NaCl		optimum	0	%(w/v)

[Ref.: #66606]	Observation	Cell density is highest at 0-10°C.
[Ref.: #66606]	Observation	Utilization of N-acetyl-b-D-glucosamine, glucosamine, D-glucose, maltose, L-glutamine, L-asparagine, L-proline and casamino acids in the presence of yeast extract (100 mg l-1) in mineral medium and for low resistance to 1 mM As2O5 .

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #66606]	acetic acid ChEBI	-	carbon source
[Ref.: #66606]	benzoate ChEBI	-	carbon source
[Ref.: #66606]	cellobiose ChEBI	-	carbon source
[Ref.: #66606]	D-fructose ChEBI	-	carbon source
[Ref.: #66606]	D-glucose ChEBI	-	carbon source
[Ref.: #66606]	D-mannose ChEBI	-	carbon source
[Ref.: #66606]	D-xylose ChEBI	-	carbon source
[Ref.: #66606]	fumarate ChEBI	-	carbon source
[Ref.: #66606]	galactose ChEBI	-	carbon source
[Ref.: #66606]	glucosamine ChEBI	-	carbon source
[Ref.: #66606]	glycerol ChEBI	-	carbon source
[Ref.: #66606]	glycine ChEBI	-	carbon source
[Ref.: #66606]	L-arabinose ChEBI	-	carbon source
[Ref.: #66606]	lactate ChEBI	-	carbon source
[Ref.: #66606]	lactose ChEBI	-	carbon source
[Ref.: #66606]	maltose ChEBI	-	carbon source
[Ref.: #66606]	N-acetylglucosamine ChEBI	-	carbon source
[Ref.: #66606]	nitrate ChEBI	-	reduction
[Ref.: #66606]	phenylalanine ChEBI	-	carbon source
[Ref.: #66606]	pyruvate ChEBI	-	carbon source
[Ref.: #66606]	succinamate ChEBI	-	carbon source
[Ref.: #66606]	sucrose ChEBI	-	carbon source
[Ref.: #66606]	trehalose ChEBI	-	carbon source
[Ref.: #66606]	yeast extract	+	carbon source
	Only first 10 entries are displayed. Click here to see all.

	Metabolite production	Metabolite	Production
[Ref.: #66606]		indole ChEBI	no

	Enzyme	Enzyme activity	EC number
[Ref.: #66606]	acid phosphatase	+	3.1.3.2
[Ref.: #66606]	alkaline phosphatase	+	3.1.3.1
[Ref.: #66606]	alpha-chymotrypsin	-	3.4.21.1
[Ref.: #66606]	alpha-fucosidase	-	3.2.1.51
[Ref.: #66606]	alpha-galactosidase	-	3.2.1.22
[Ref.: #66606]	alpha-glucosidase	+	3.2.1.20
[Ref.: #66606]	alpha-mannosidase	-	3.2.1.24
[Ref.: #66606]	arginine dihydrolase	-	3.5.3.6
[Ref.: #66606]	beta-galactosidase	-	3.2.1.23
[Ref.: #66606]	beta-glucosidase	-	3.2.1.21
[Ref.: #66606]	beta-glucuronidase	-	3.2.1.31
[Ref.: #66606]	catalase	+	1.11.1.6
[Ref.: #66606]	cystine arylamidase	-	3.4.11.3
[Ref.: #66606]	cytochrome oxidase	+	1.9.3.1
[Ref.: #66606]	esterase (C 4)	+
[Ref.: #66606]	esterase Lipase (C 8)	-
[Ref.: #66606]	gelatinase	-
[Ref.: #66606]	leucine arylamidase	+	3.4.11.1
[Ref.: #66606]	lipase (C 14)	-
[Ref.: #66606]	N-acetyl-beta-glucosaminidase	-	3.2.1.52
[Ref.: #66606]	naphthol-AS-BI-phosphohydrolase	+
[Ref.: #66606]	trypsin	-	3.4.21.4
[Ref.: #66606]	urease	-	3.5.1.5
[Ref.: #66606]	valine arylamidase	-
	Only first 10 entries are displayed. Click here to see all.

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #66606]	Sample type/isolated from	alpine forest soil (2000 m above sea level)
[Ref.: #66606]	Sampling date	2014
[Ref.: #66606]	Geographic location (country and/or sea, region)	Schwarzseespitze/Ritten in South Tyrol
[Ref.: #66606]	Country	Italy
[Ref.: #66606]	Country ISO 3 Code	ITA
[Ref.: #66606]	Continent	Europe
[Ref.: #66606]	Enrichment culture	R2A agar
[Ref.: #66606]	Enrichment culture composition	amended with cycloheximide to exclude fungal growth
[Ref.: #66606]	Enrichment culture temperature	20 ̊C
[Ref.: #66606]	Isolation/enrichment procedure	Ten grams of soil sample were shaken with 90 ml sterile 0.1 % sodium pyrophosphate for 15 min at 150 r.p.m. Appropriate dilutions, prepared with sterile saline solution (0.9 % NaCl w/v), were plated on R2A agar amended with cycloheximide to exclude fungal growth and were incubated at 20°C.
* marker position based on {}

Isolation sources categories

#Climate	#Cold	#Alpine
#Environmental	#Terrestrial	#Forest
#Environmental	#Terrestrial	#Soil

What are isolation sources categories?

[Ref.: #69479]

Global distribution of 16S sequence KP899250 (>99% sequence identity) for Solimicrobium silvestre subclade from Microbeatlas

Aquatic Samples	20
Soil Samples	26
Animal Samples	8
Plant Samples	1
Total Samples	55

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #66606]	Herminiimonas sp. S20-91 16S ribosomal RNA gene, partial sequence	KP899250	1342	ENA	2099400 tax ID

Genome sequence information:

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #66792]	Solimicrobium silvestre S20-91	GCA_002976435	contig	GenBank	2099400 tax ID	*
[Ref.: #66792]	Solimicrobium silvestre S20-91	2832032608	draft	JGI IMG/M	2099400 tax ID	*

[Ref.: #66606]

GC-content

46.7 mol%

genome sequence analysis

Availability in culture collections External links

[Ref.: #66606]

Culture collection no.

DSM 104733, LMG 30010

[Ref.: #112168]

SI-ID 407522

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Sapientia aquatica gen. nov., sp. nov., isolated from a crater lake.	Felfoldi T, Szabo A, Toth E, Schumann P, Keki Z, Marialigeti K, Mathe I	Int J Syst Evol Microbiol	10.1099/ijsem.0.003761	2020	*
Phylogeny	Solimicrobium silvestre gen. nov., sp. nov., isolated from alpine forest soil.	Margesin R, Albuquerque L, Zhang DC, Froufe HJC, Severino R, Roxo I, Egas C, da Costa MS	Int J Syst Evol Microbiol	10.1099/ijsem.0.002861	2018	*

References

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#66606

Rosa Margesin, Luciana Albuquerque, De-Chao Zhang, Hugo J. C. Froufe, Rita Severino, Inês Roxo, Conceicao Egas, Milton S. da Costa: Solimicrobium silvestre gen. nov., sp. nov., isolated from alpine forest soil. IJSEM 68: 2491 - 2498 2018 ( DOI 10.1099/ijsem.0.002861 , PubMed 29939122 )

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#69031

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 104733

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

#112168

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID407522.1 )

* These data were automatically processed and therefore are not curated

Change proposal

You found an error in BacDive? Please tell us about it!

Note that changes will be reviewed and judged. If your changes are legitimate, changes will occur within the next BacDive update. Only proposed changes supported by the according reference will be reviewed. The BacDive team reserves the right to reject proposed changes.

Search for species Solimicrobium silvestre in external resources:
SILVA
BRENDA
PANGAEA
StrainInfo
GBIF