Caballeronia insecticola (NCIMB 15023, JCM 31142, RPE64)

Name: Caballeronia insecticola (NCIMB 15023, JCM 31142, RPE64)
Creator: BacDive
License: http://creativecommons.org/licenses/by/4.0/

BacDive ID 159714

Type strain

Strain Designation RPE64

Culture col. no. NCIMB 15023 JCM 31142 RPE64

NCBI tax ID(s) 758793

Special Collections JCM Literature strains

History Y. Kikuchi; Bioprod. Res. Inst., AIST, Japan; RPE64.

Links

Caballeronia insecticola RPE64 is an aerobe, Gram-negative, motile bacterium that forms circular colonies and was isolated from midgut crypts of the bean bug, Riptortus pedestris, from a pea field.

Gram-negative motile rod-shaped colony-forming aerobe genome sequence 16S sequence Bacteria

Name and taxonomic classification

SI-ID 272768

LMG 29948,JCM 31142,NCIMB 15023

@ref 20215

Last LPSN update 2026-02-09 11:38:43

Domain Bacteria

Phylum Pseudomonadota

Class Betaproteobacteria

Order Burkholderiales

Family Burkholderiaceae

Genus Caballeronia

Species Caballeronia insecticola

Full scientific name Caballeronia insecticola (Takeshita et al. 2018) Dobritsa and Samadpour 2019

Synonyms (1)

Burkholderia insecticola

Morphology

Cell morphology

@ref	Gram stain	Cell length	Cell width	Cell shape	Flagellum arrangement	Confidence
66600	negative	2.4-3.2 µm	0.9 µm	rod-shaped	polar
125439	negative					91.5
125438	negative					98.482

Colony morphology

@ref	Colony color	Colony shape	Medium used
66600	white-creamy	circular	yeast glucose agar

Culture and growth conditions

Culture medium

@ref	Name	Growth
66600	MacConkey agar
66600	yeast glucose agar

Culture temp

@ref	Growth	Type	Temperature (°C)
66600	positive	optimum	30
66600	positive	growth	15-35
67770	positive	growth	30

Culture pH

@ref	Ability	Type	PH
66600	positive	growth	6-8

Physiology and metabolism

Oxygen tolerance

@ref	Oxygen tolerance	Confidence
66600	aerobe
125438	aerobe	93.012
125439	obligate aerobe	99.7

Spore formation

@ref	Spore formation	Confidence
66600
125439		92.5

Halophily

@ref	Salt	Growth	Tested relation	Concentration
66600	NaCl	positive	growth	0-2.5 %(w/v)
66600	NaCl	positive	optimum	1.5 %(w/v)

Observation

66600

Observationmotile by two or three polar flagella

Metabolite utilization

@ref	Chebi-ID	Metabolite	Utilization activity	Kind of utilization tested
66600	17128 ChEBI	adipate	-	assimilation
66600	22599 ChEBI	arabinose	+	assimilation
66600		casein	-	hydrolysis
66600	53258 ChEBI	citric acid	-	assimilation
66600	27689 ChEBI	decanoate	+	assimilation
66600	4853 ChEBI	esculin	-	hydrolysis
66600	5291 ChEBI	gelatin	-	hydrolysis
66600	24265 ChEBI	gluconate	+	assimilation
66600	17234 ChEBI	glucose	+	assimilation
66600	17234 ChEBI	glucose	-	fermentation
66600	25115 ChEBI	malate	+	assimilation
66600	17306 ChEBI	maltose	-	assimilation
66600	29864 ChEBI	mannitol	+	assimilation
66600	37684 ChEBI	mannose	+	assimilation
66600	59640 ChEBI	N-acetylglucosamine	+	assimilation
66600	17632 ChEBI	nitrate	-	reduction
66600	18401 ChEBI	phenylacetate	+	assimilation
66600	28017 ChEBI	starch	-	hydrolysis
66600	53424 ChEBI	tween 20	-	growth
66600	53424 ChEBI	tween 20	-	hydrolysis
66600	53423 ChEBI	tween 40	+	growth
66600	53423 ChEBI	tween 40	-	hydrolysis
66600	53425 ChEBI	tween 60	+	growth
66600	53425 ChEBI	tween 60	+	hydrolysis
66600	53426 ChEBI	tween 80	-	growth
66600	53426 ChEBI	tween 80	-	hydrolysis

Enzymes

@ref	Value	Activity	Ec
66600	acid phosphatase	+	3.1.3.2
66600	alkaline phosphatase	-	3.1.3.1
66600	alpha-fucosidase	-	3.2.1.51
66600	alpha-galactosidase	-	3.2.1.22
66600	alpha-glucosidase	-	3.2.1.20
66600	alpha-mannosidase	-	3.2.1.24
66600	arginine dihydrolase	-	3.5.3.6
66600	beta-galactosidase	-	3.2.1.23
66600	beta-glucosidase	-	3.2.1.21
66600	beta-glucuronidase	-	3.2.1.31
66600	catalase	+	1.11.1.6
66600	chymotrypsin	-	3.4.4.5
66600	cystine arylamidase	-	3.4.11.3
66600	cytochrome oxidase	-	1.9.3.1
66600	DNase	-
66600	esterase (C 4)	+
66600	esterase Lipase (C 8)	-
66600	leucine arylamidase	-	3.4.11.1
66600	lipase (C 14)	+
66600	N-acetyl-beta-glucosaminidase	-	3.2.1.52
66600	phosphoamidase	-	3.9.1.1
66600	trypsin	-	3.4.21.4
66600	tryptophanase	-	4.1.99.1
66600	urease	-	3.5.1.5
66600	valine arylamidase	-

Isolation, sampling and environmental information

Isolation source categories

Cat1	Cat2	Cat3
#Engineered	#Agriculture	#Field
#Host	#Arthropoda	#Insecta
#Host Body-Site	#Gastrointestinal tract	-

Isolation

@ref	Sample type	Host species	Sampling date	Geographic location	Country	Country ISO 3 Code	Continent	Enrichment culture	Enrichment culture composition	Enrichment culture duration	Enrichment culture temperature
66600	midgut crypts of the bean bug, Riptortus pedestris, from a pea field	Riptortus pedestris	2007	Tsukuba	Japan	JPN	Asia	yeast glucose agar	(0.5 % yeast extract, 0.4 % glucose, 0.1 % NaCl and 1.5 % agar, pH 7.0)	3 days	26
67770	Midgut crypts of the bean bug, Riptortus pedestris, Japan in 2007	Riptortus pedestris		Tsukuba	Japan	JPN	Asia

Interaction and safety

Sequence information

Genome sequences

@ref	Description	Assembly level	INSDC accession	BV-BRC accession	IMG accession	NCBI tax ID	Score
66792	ASM40203v1 assembly for Caballeronia insecticola RPE64	complete	GCA_000402035	758793.3	2597489944	758793	96.3

16S sequences

@ref	Description	Accession	Length	Database	NCBI tax ID
66600	Burkholderia insecticola gene for 16S rRNA, partial sequence	AB558208	1456		758793

GC content

@ref	GC-content (mol%)	Method
66600	63.2	genome sequence analysis

Genome-based predictions

Predictions

Predictions from Deep Learning for Genome Sequence Data. R package version 0.3.1 based on: Caballeronia insecticola RPE64
IMG: 2773857905

@ref	Trait	Model	Prediction	Confidence in %	In training data
125439	spore_formation	BacteriaNetⓘ	no	92.50	no
125439	motility	BacteriaNetⓘ	yes	56.90	no
125439	gram_stain	BacteriaNetⓘ	negative	91.50	no
125439	oxygen_tolerance	BacteriaNetⓘ	obligate aerobe	99.70	no

Predictions from bacterial phenotypic traits through improved machine learning using high-quality, curated datasets based on: Caballeronia insecticola RPE64
NCBI: GCA_000402035.1

@ref	Trait	Model	Prediction	Confidence in %	In training data
125438	gram-positive	gram-positiveⓘ	no	98.48	yes
125438	anaerobic	anaerobicⓘ	no	97.91	yes
125438	aerobic	aerobicⓘ	yes	93.01	yes
125438	spore-forming	spore-formingⓘ	no	88.76	no
125438	thermophilic	thermophileⓘ	no	97.50	yes
125438	flagellated	motile2+ⓘ	yes	84.16	yes

Literature

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Reclassification of Burkholderia insecticola as Caballeronia insecticola comb. nov. and reliability of conserved signature indels as molecular synapomorphies.	Dobritsa AP, Samadpour M	Int J Syst Evol Microbiol	10.1099/ijsem.0.003431	2019
Phylogeny	Burkholderia insecticola sp. nov., a gut symbiotic bacterium of the bean bug Riptortus pedestris.	Takeshita K, Tamaki H, Ohbayashi T, Meng XY, Sone T, Mitani Y, Peeters C, Kikuchi Y, Vandamme P	Int J Syst Evol Microbiol	10.1099/ijsem.0.002848	2018

References

#20215	Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )
#66600	Kazutaka Takeshita, Hideyuki Tamaki, Tsubasa Ohbayashi, Xian-Ying Meng, Teruo Sone, Yasuo Mitani, Charlotte Peeters, Yoshitomo Kikuchi, Peter Vandamme: Burkholderia insecticola sp. nov., a gut symbiotic bacterium of the bean bug Riptortus pedestris. IJSEM 68: 2370 - 2374 2018 ( DOI 10.1099/ijsem.0.002848 , PubMed 29863457 )
#66792	Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .
#67770	Japan Collection of Microorganism (JCM) ; Curators of the JCM;
#125438	Julia Koblitz, Lorenz Christian Reimer, Rüdiger Pukall, Jörg Overmann: Predicting bacterial phenotypic traits through improved machine learning using high-quality, curated datasets. 2024 ( DOI 10.1101/2024.08.12.607695 )
#125439	Philipp Münch, René Mreches, Martin Binder, Hüseyin Anil Gündüz, Xiao-Yin To, Alice McHardy: deepG: Deep Learning for Genome Sequence Data. R package version 0.3.1 .
#126262	A. Lissin, I. Schober, J. F. Witte, H. Lüken, A. Podstawka, J. Koblitz, B. Bunk, P. Dawyndt, P. Vandamme, P. de Vos, J. Overmann, L. C. Reimer: StrainInfo—the central database for linked microbial strain identifiers. ( DOI 10.1093/database/baaf059 )

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Caballeronia insecticola (NCIMB 15023, JCM 31142, RPE64)

Name and taxonomic classification

Morphology

Cell morphology

Colony morphology

Culture and growth conditions

Culture medium

Culture temp

Culture pH

Physiology and metabolism

Oxygen tolerance

Spore formation

Halophily

Observation

Metabolite utilization

Enzymes

Isolation, sampling and environmental information

Isolation source categories

Isolation

Interaction and safety

Sequence information

Genome sequences

Genome browser: GCA_000402035

16S sequences

GC content

Genome-based predictions

Predictions

Literature

Literature

References

BacDive

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