Name: Caballeronia insecticola RPE64
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 159714

Type strain:

Species: Caballeronia insecticola

Strain Designation: RPE64

Culture col. no.: NCIMB 15023, JCM 31142

Strain history: Y. Kikuchi; Bioprod. Res. Inst., AIST, Japan; RPE64.

NCBI tax ID(s): 758793 (species)

Section

Caballeronia insecticola RPE64 is an aerobe, mesophilic, Gram-negative bacterium that forms circular colonies and was isolated from midgut crypts of the bean bug, Riptortus pedestris, from a pea field.

colony-forming
Gram-negative
motile
rod-shaped
aerobe
mesophilic
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Pseudomonadota*
	Class	*Betaproteobacteria*
	Order	*Burkholderiales*
	Family	*Burkholderiaceae*
	Genus	*Caballeronia*
	Species	**Caballeronia insecticola**
	Full Scientific Name (LPSN)	Caballeronia insecticola (Takeshita et al. 2018) Dobritsa and Samadpour 2019

Synonym

Burkholderia insecticola

Information on morphological and physiological properties Morphology

[Ref.: #66600]	Gram stain	negative
[Ref.: #69480]	Gram stain	negative Confidence in %99.988

[Ref.: #66600]	Cell length	2.4-3.2 µm

[Ref.: #66600]	Cell width	0.9 µm

[Ref.: #66600]	Cell shape	rod-shaped

[Ref.: #66600]	Motility	yes
[Ref.: #69480]	Motility	yes Confidence in %92.821

[Ref.: #66600]	Flagellum arrangement	polar

[Ref.: #66600]	Colony color	white-creamy
[Ref.: #66600]	Colony shape	circular
[Ref.: #66600]	Cultivation medium used	yeast glucose agar

Information on culture and growth conditions Culture and growth conditions

[Ref.: #66600]

Culture medium

MacConkey agar

[Ref.: #66600]

Culture medium growth

[Ref.: #66600]

Culture medium

yeast glucose agar

[Ref.: #66600]

Culture medium growth

		Temperature
[Ref.: #66600]	optimum	30 °C
[Ref.: #66600]	growth	15-35 °C
[Ref.: #67770]	growth	30 °C

[Ref.: #66600]	Temperature range	mesophilic
[Ref.: #67770]	Temperature range	mesophilic

	pH	Kind of pH		pH
[Ref.: #66600]			growth	6-8

Information on physiology and metabolism Physiology and metabolism

[Ref.: #66600]

Oxygen tolerance

aerobe

[Ref.: #66600]	Ability of spore formation	no
[Ref.: #69480]	Ability of spore formation	no Confidence in %99.983
[Ref.: #69481]	Ability of spore formation	no Confidence in %100

	Halophily	Salt	Tested relation		Salt conc.
[Ref.: #66600]		NaCl		growth	0-2.5	%(w/v)
[Ref.: #66600]		NaCl		optimum	1.5	%(w/v)

[Ref.: #66600]

Observation

motile by two or three polar flagella

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #66600]	adipate ChEBI	-	assimilation
[Ref.: #66600]	arabinose ChEBI	+	assimilation
[Ref.: #66600]	casein	-	hydrolysis
[Ref.: #66600]	citric acid ChEBI	-	assimilation
[Ref.: #66600]	decanoate ChEBI	+	assimilation
[Ref.: #66600]	esculin ChEBI	-	hydrolysis
[Ref.: #66600]	gelatin ChEBI	-	hydrolysis
[Ref.: #66600]	gluconate ChEBI	+	assimilation
[Ref.: #66600]	glucose ChEBI	+	assimilation
[Ref.: #66600]	glucose ChEBI	-	fermentation
[Ref.: #66600]	malate ChEBI	+	assimilation
[Ref.: #66600]	maltose ChEBI	-	assimilation
[Ref.: #66600]	mannitol ChEBI	+	assimilation
[Ref.: #66600]	mannose ChEBI	+	assimilation
[Ref.: #66600]	N-acetylglucosamine ChEBI	+	assimilation
[Ref.: #66600]	nitrate ChEBI	-	reduction
[Ref.: #66600]	phenylacetate ChEBI	+	assimilation
[Ref.: #66600]	starch ChEBI	-	hydrolysis
[Ref.: #66600]	tween 20 ChEBI	-	growth
[Ref.: #66600]	tween 20 ChEBI	-	hydrolysis
[Ref.: #66600]	tween 40 ChEBI	+	growth
[Ref.: #66600]	tween 40 ChEBI	-	hydrolysis
[Ref.: #66600]	tween 60 ChEBI	+	growth
[Ref.: #66600]	tween 60 ChEBI	+	hydrolysis
[Ref.: #66600]	tween 80 ChEBI	-	growth
[Ref.: #66600]	tween 80 ChEBI	-	hydrolysis
	Only first 10 entries are displayed. Click here to see all.

	Enzyme	Enzyme activity	EC number
[Ref.: #66600]	acid phosphatase	+	3.1.3.2
[Ref.: #66600]	alkaline phosphatase	-	3.1.3.1
[Ref.: #66600]	alpha-fucosidase	-	3.2.1.51
[Ref.: #66600]	alpha-galactosidase	-	3.2.1.22
[Ref.: #66600]	alpha-glucosidase	-	3.2.1.20
[Ref.: #66600]	alpha-mannosidase	-	3.2.1.24
[Ref.: #66600]	arginine dihydrolase	-	3.5.3.6
[Ref.: #66600]	beta-galactosidase	-	3.2.1.23
[Ref.: #66600]	beta-glucosidase	-	3.2.1.21
[Ref.: #66600]	beta-glucuronidase	-	3.2.1.31
[Ref.: #66600]	catalase	+	1.11.1.6
[Ref.: #66600]	chymotrypsin	-	3.4.4.5
[Ref.: #66600]	cystine arylamidase	-	3.4.11.3
[Ref.: #66600]	cytochrome oxidase	-	1.9.3.1
[Ref.: #66600]	DNase	-
[Ref.: #66600]	esterase (C 4)	+
[Ref.: #66600]	esterase Lipase (C 8)	-
[Ref.: #66600]	leucine arylamidase	-	3.4.11.1
[Ref.: #66600]	lipase (C 14)	+
[Ref.: #66600]	N-acetyl-beta-glucosaminidase	-	3.2.1.52
[Ref.: #66600]	phosphoamidase	-	3.9.1.1
[Ref.: #66600]	trypsin	-	3.4.21.4
[Ref.: #66600]	tryptophanase	-	4.1.99.1
[Ref.: #66600]	urease	-	3.5.1.5
[Ref.: #66600]	valine arylamidase	-
	Only first 10 entries are displayed. Click here to see all.

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #66600]	Sample type/isolated from	midgut crypts of the bean bug, Riptortus pedestris, from a pea field
[Ref.: #66600]	Host species	Riptortus pedestris
[Ref.: #66600]	Sampling date	2007
[Ref.: #66600]	Geographic location (country and/or sea, region)	Tsukuba
[Ref.: #66600]	Country	Japan
[Ref.: #66600]	Country ISO 3 Code	JPN
[Ref.: #66600]	Continent	Asia
[Ref.: #66600]	Enrichment culture	yeast glucose agar
[Ref.: #66600]	Enrichment culture composition	(0.5 % yeast extract, 0.4 % glucose, 0.1 % NaCl and 1.5 % agar, pH 7.0)
[Ref.: #66600]	Enrichment culture duration	3 days
[Ref.: #66600]	Enrichment culture temperature	26 ̊C

[Ref.: #67770]	Sample type/isolated from	Midgut crypts of the bean bug, Riptortus pedestris, Japan in 2007
[Ref.: #67770]	Host species	Riptortus pedestris
[Ref.: #67770]	Geographic location (country and/or sea, region)	Tsukuba
[Ref.: #67770]	Country	Japan
[Ref.: #67770]	Country ISO 3 Code	JPN
[Ref.: #67770]	Continent	Asia
* marker position based on {}

Isolation sources categories

#Engineered	#Agriculture	#Field
#Host	#Arthropoda	#Insecta
#Host Body-Site	#Gastrointestinal tract	-

What are isolation sources categories?

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #66600]	Burkholderia insecticola gene for 16S rRNA, partial sequence	AB558208	1456	ENA	758793 tax ID

Genome sequence information:

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #66792]	Caballeronia insecticola RPE64	GCA_000402035	complete	GenBank	758793 tax ID	*
[Ref.: #66792]	Caballeronia insecticola RPE64	2773857905	complete	JGI IMG/M	758793 tax ID	*
[Ref.: #66792]	Caballeronia insecticola RPE64	2597489944	complete	JGI IMG/M	758793 tax ID	*

[Ref.: #66600]

GC-content

63.2 mol%

genome sequence analysis

Data predicted using genome information as a basis Genome-based predictions

	Trait	Prediction	Confidence in %	In training data
	Predictions from GenomeNet Sporulation v. 1 based on: Caballeronia insecticola RPE64 NCBI: GCA_000402035.1
[Ref.: #69481]	spore-forming	no	100	no

	Trait	Prediction	Confidence in %	In training data
	Predictions from Diaspora project based on: Caballeronia insecticola RPE64 NCBI: GCA_000402035.1
[Ref.: #69480]	motile	yes	90.729	yes
[Ref.: #69480]	flagellated	no	81.142	no
[Ref.: #69480]	gram-positive	no	97.931	yes
[Ref.: #69480]	anaerobic	no	98.69	yes
[Ref.: #69480]	aerobic	yes	90.986	yes
[Ref.: #69480]	halophile	no	93.483	yes
[Ref.: #69480]	spore-forming	no	94.286	yes
[Ref.: #69480]	thermophile	no	99.476	no
[Ref.: #69480]	glucose-util	yes	89.05	yes
[Ref.: #69480]	glucose-ferment	no	90.224	yes

Availability in culture collections External links

[Ref.: #66600]

Culture collection no.

NCIMB 15023, JCM 31142

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Burkholderia insecticola sp. nov., a gut symbiotic bacterium of the bean bug Riptortus pedestris.	Takeshita K, Tamaki H, Ohbayashi T, Meng XY, Sone T, Mitani Y, Peeters C, Kikuchi Y, Vandamme P	Int J Syst Evol Microbiol	10.1099/ijsem.0.002848	2018	*
Phylogeny	Reclassification of Burkholderia insecticola as Caballeronia insecticola comb. nov. and reliability of conserved signature indels as molecular synapomorphies.	Dobritsa AP, Samadpour M	Int J Syst Evol Microbiol	10.1099/ijsem.0.003431	2019	*

References

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#66600

Kazutaka Takeshita, Hideyuki Tamaki, Tsubasa Ohbayashi, Xian-Ying Meng, Teruo Sone, Yasuo Mitani, Charlotte Peeters, Yoshitomo Kikuchi, Peter Vandamme: Burkholderia insecticola sp. nov., a gut symbiotic bacterium of the bean bug Riptortus pedestris. IJSEM 68: 2370 - 2374 2018 ( DOI 10.1099/ijsem.0.002848 , PubMed 29863457 )

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#67770 Japan Collection of Microorganism (JCM) ; Curators of the JCM;

#69480

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#69481

Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch: Predictions based on the model GenomeNet Sporulation v. 1 . ( DOI 10.21203/rs.3.rs-2527258/v1 )

* These data were automatically processed and therefore are not curated

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