Name: Oleiharenicola alkalitolerans NVT
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 159703

Type strain:

Species: Oleiharenicola alkalitolerans

Strain Designation: NVT

Culture col. no.: ATCC BAA-2697, DSM 29249

Strain history: <- P. Dunfield, Univ. Calgary, Canada; NVT

NCBI tax ID(s): 2100748 (species)

SI-ID 402978

DSM 29249

Section

Oleiharenicola alkalitolerans NVT is an aerobe, chemoorganoheterotroph, Gram-negative bacterium that forms circular colonies and was isolated from oilsands process-affected tailings water sample from 0 to 10 cm below the surface and about 15 m from the shore.

coccus-shaped
colony-forming
Gram-negative
aerobe
chemoorganoheterotroph
Bacteria

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Verrucomicrobiota*
	Class	*Opitutia*
	Order	*Opitutales*
	Family	*Opitutaceae*
	Genus	*Oleiharenicola*
	Species	**Oleiharenicola alkalitolerans**
	Full Scientific Name (LPSN)	Oleiharenicola alkalitolerans Rochman et al. 2018

Information on morphological and physiological properties Morphology

[Ref.: #66550]	Gram stain	negative

[Ref.: #66550]	Cell length	0.5-1 µm

[Ref.: #66550]	Cell shape	coccus-shaped
[Ref.: #66550]	Cell shape	diplococcus-shaped

[Ref.: #66550]	Motility	no

[Ref.: #66550]	Colony size	0.5-4 mm
[Ref.: #66550]	Colony color	pale white
[Ref.: #66550]	Colony shape	circular
[Ref.: #66550]	Cultivation medium used	20% R2A

Information on culture and growth conditions Culture and growth conditions

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Culture medium

20% R2A

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Culture medium growth

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Culture medium

R2A MEDIUM (DSMZ Medium 830)

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Culture medium growth

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Culture medium link

Medium recipe at MediaDive

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Culture medium composition

expand / minimize

		Temperature
[Ref.: #66550]	growth	15-40 °C
[Ref.: #66550]	optimum	25-37 °C
[Ref.: #67699]	growth	28 °C

[Ref.: #66550]	Temperature range	mesophilic
[Ref.: #67699]	Temperature range	mesophilic

	pH	Kind of pH		pH
[Ref.: #66550]			optimum	6-8
[Ref.: #66550]			growth	5.5-11

Information on physiology and metabolism Physiology and metabolism

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Oxygen tolerance

aerobe

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Nutrition type

chemoorganoheterotroph

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Ability of spore formation

	Halophily	Salt	Tested relation		Salt conc.
[Ref.: #66550]		NaCl		growth	0-3	%(w/v)

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Observation

Doubling time under optimum conditions is 0.6 h.

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #66550]	adipate ChEBI	-	assimilation
[Ref.: #66550]	amygdalin ChEBI	-	builds acid from
[Ref.: #66550]	arabinose ChEBI	+	assimilation
[Ref.: #66550]	arbutin ChEBI	-	builds acid from
[Ref.: #66550]	catechol ChEBI	-	growth
[Ref.: #66550]	cellobiose ChEBI	-	builds acid from
[Ref.: #66550]	D-arabinose ChEBI	-	builds acid from
[Ref.: #66550]	D-arabitol ChEBI	+	builds acid from
[Ref.: #66550]	D-fructose ChEBI	+	builds acid from
[Ref.: #66550]	D-fucose ChEBI	+	builds acid from
[Ref.: #66550]	D-galactose ChEBI	+	builds acid from
[Ref.: #66550]	D-glucose ChEBI	+	builds acid from
[Ref.: #66550]	D-lyxose ChEBI	-	builds acid from
[Ref.: #66550]	D-mannitol ChEBI	+	builds acid from
[Ref.: #66550]	D-mannose ChEBI	-	builds acid from
[Ref.: #66550]	D-ribose ChEBI	-	builds acid from
[Ref.: #66550]	D-sorbitol ChEBI	+	builds acid from
[Ref.: #66550]	D-tagatose ChEBI	-	builds acid from
[Ref.: #66550]	D-xylose ChEBI	+	builds acid from
[Ref.: #66550]	decanoate ChEBI	-	assimilation
[Ref.: #66550]	dinitrogen ChEBI	-	nitrogen source
[Ref.: #66550]	erythritol ChEBI	-	builds acid from
[Ref.: #66550]	esculin ferric citrate	+	builds acid from
[Ref.: #66550]	galactitol ChEBI	-	builds acid from
[Ref.: #66550]	gelatin ChEBI	-	hydrolysis
[Ref.: #66550]	gentiobiose ChEBI	-	builds acid from
[Ref.: #66550]	glucose ChEBI	+	assimilation
[Ref.: #66550]	glucose ChEBI	-	fermentation
[Ref.: #66550]	glycerol ChEBI	-	builds acid from
[Ref.: #66550]	glycine ChEBI	+	nitrogen source
[Ref.: #66550]	glycogen ChEBI	-	builds acid from
[Ref.: #66550]	inulin ChEBI	-	builds acid from
[Ref.: #66550]	L-alanine ChEBI	+	nitrogen source
[Ref.: #66550]	L-arabinose ChEBI	-	builds acid from
[Ref.: #66550]	L-arabitol ChEBI	-	builds acid from
[Ref.: #66550]	L-fucose ChEBI	-	builds acid from
[Ref.: #66550]	L-proline ChEBI	+	nitrogen source
[Ref.: #66550]	L-rhamnose ChEBI	-	builds acid from
[Ref.: #66550]	L-serine ChEBI	+	nitrogen source
[Ref.: #66550]	L-sorbose ChEBI	-	builds acid from
[Ref.: #66550]	L-valine ChEBI	-	nitrogen source
[Ref.: #66550]	L-xylose ChEBI	-	builds acid from
[Ref.: #66550]	lactose ChEBI	-	builds acid from
[Ref.: #66550]	malate ChEBI	+	assimilation
[Ref.: #66550]	maltose ChEBI	-	assimilation
[Ref.: #66550]	maltose ChEBI	-	builds acid from
[Ref.: #66550]	mannitol ChEBI	+	assimilation
[Ref.: #66550]	mannose ChEBI	-	assimilation
[Ref.: #66550]	melezitose ChEBI	-	builds acid from
[Ref.: #66550]	melibiose ChEBI	-	builds acid from
[Ref.: #66550]	methyl alpha-D-glucopyranoside ChEBI	-	builds acid from
[Ref.: #66550]	methyl alpha-D-mannoside ChEBI	-	builds acid from
[Ref.: #66550]	methyl beta-D-xylopyranoside ChEBI	-	builds acid from
[Ref.: #66550]	myo-inositol ChEBI	-	builds acid from
[Ref.: #66550]	N-acetylglucosamine ChEBI	-	assimilation
[Ref.: #66550]	N-acetylglucosamine ChEBI	-	builds acid from
[Ref.: #66550]	nitrate ChEBI	-	reduction
[Ref.: #66550]	phenylacetate ChEBI	-	assimilation
[Ref.: #66550]	potassium 2-dehydro-D-gluconate	-	builds acid from
[Ref.: #66550]	potassium 5-dehydro-D-gluconate	-	builds acid from
[Ref.: #66550]	potassium gluconate ChEBI	-	assimilation
[Ref.: #66550]	potassium gluconate ChEBI	-	builds acid from
[Ref.: #66550]	raffinose ChEBI	-	builds acid from
[Ref.: #66550]	ribitol ChEBI	-	builds acid from
[Ref.: #66550]	salicin ChEBI	-	builds acid from
[Ref.: #66550]	sodium chloride ChEBI	-	required for growth
[Ref.: #66550]	sodium citrate ChEBI	-	assimilation
[Ref.: #66550]	sodium nitrate ChEBI	+	nitrogen source
[Ref.: #66550]	starch ChEBI	-	builds acid from
[Ref.: #66550]	sucrose ChEBI	-	builds acid from
[Ref.: #66550]	trehalose ChEBI	-	builds acid from
[Ref.: #66550]	turanose ChEBI	-	builds acid from
[Ref.: #66550]	urea ChEBI	+	nitrogen source
[Ref.: #66550]	xylitol ChEBI	+	builds acid from
	Only first 10 entries are displayed. Click here to see all.

	Metabolite production	Metabolite	Production
[Ref.: #66550]		indole ChEBI	no

	Enzyme	Enzyme activity	EC number
[Ref.: #66550]	acid phosphatase	+	3.1.3.2
[Ref.: #66550]	alkaline phosphatase	+	3.1.3.1
[Ref.: #66550]	alpha-chymotrypsin	+	3.4.21.1
[Ref.: #66550]	alpha-fucosidase	-	3.2.1.51
[Ref.: #66550]	alpha-galactosidase	-	3.2.1.22
[Ref.: #66550]	alpha-glucosidase	-	3.2.1.20
[Ref.: #66550]	alpha-mannosidase	-	3.2.1.24
[Ref.: #66550]	arginine dihydrolase	-	3.5.3.6
[Ref.: #66550]	beta-galactosidase	+/-	3.2.1.23
[Ref.: #66550]	beta-glucosidase	+/-	3.2.1.21
[Ref.: #66550]	beta-glucuronidase	-	3.2.1.31
[Ref.: #66550]	catalase	+	1.11.1.6
[Ref.: #66550]	cystine arylamidase	-	3.4.11.3
[Ref.: #66550]	cytochrome oxidase	-	1.9.3.1
[Ref.: #66550]	esterase (C 4)	-
[Ref.: #66550]	esterase Lipase (C 8)	+
[Ref.: #66550]	leucine arylamidase	-	3.4.11.1
[Ref.: #66550]	lipase (C 14)	-
[Ref.: #66550]	N-acetyl-beta-glucosaminidase	-	3.2.1.52
[Ref.: #66550]	naphthol-AS-BI-phosphohydrolase	+
[Ref.: #66550]	trypsin	+	3.4.21.4
[Ref.: #66550]	urease	+	3.5.1.5
[Ref.: #66550]	valine arylamidase	-
	Only first 10 entries are displayed. Click here to see all.

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #66550]	Sample type/isolated from	oilsands process-affected tailings water sample from 0 to 10 cm below the surface and about 15 m from the shore
[Ref.: #66550]	Sampling date	2012-06
[Ref.: #66550]	Geographic location (country and/or sea, region)	oilsands tailings pond Fort McMurray, Alberta
[Ref.: #66550]	Country	Canada
[Ref.: #66550]	Country ISO 3 Code	CAN
[Ref.: #66550]	Continent	North America
[Ref.: #66550]	Enrichment culture	mineral salts medium 10
[Ref.: #66550]	Enrichment culture composition	agar was substituted with 1.5 % phytagel and 1 % MgSO 4 (Sigma Aldrich), and deionized water was substituted with filtered sample water to mimic the native environment and provide potential energy sources
[Ref.: #66550]	Enrichment culture temperature	20-25 ̊C
[Ref.: #66550]	Isolation/enrichment procedure	To isolate the bacterium, sample water was diluted 1:10 with deionized water and 100 µl aliquots were streaked on plates of the mineral salts medium 10. Plates were inoculated with a drop of sample, streaked, and incubated at 20-25°C under air.
* marker position based on {}

Isolation sources categories

#Environmental	#Terrestrial	#Coast
#Engineered	#Contamination	#Oil (Fuel)

What are isolation sources categories?

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

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GC-content

66.1 mol%

genome sequence analysis

Availability in culture collections External links

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Culture collection no.

ATCC BAA-2697, DSM 29249

[Ref.: #112167]

SI-ID 402978

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Oleiharenicola alkalitolerans gen. nov., sp. nov., a new member of the phylum Verrucomicrobia isolated from an oilsands tailings pond.	Rochman FF, Kim JJ, Rijpstra WIC, Sinninghe Damste JS, Schumann P, Verbeke TJ, Dunfield PF	Int J Syst Evol Microbiol	10.1099/ijsem.0.002624	2018	*

References

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#66550

Fauziah F. Rochman, Joong-Jae Kim, W. Irene C. Rijpstra, Jaap S. Sinninghe Damsté, Peter Schumann, Tobin J. Verbeke, Peter F. Dunfield: Oleiharenicola alkalitolerans gen. nov., sp. nov., a new member of the phylum Verrucomicrobia isolated from an oilsands tailings pond. IJSEM 68: 1078 - 1084 2018 ( DOI 10.1099/ijsem.0.002624 , PubMed 29461179 )

#67699

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 29249

#112167

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID402978.1 )

* These data were automatically processed and therefore are not curated

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