Name: Sphingobium aquiterrae SKLS-A10
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 159646

Type strain:

Species: Sphingobium aquiterrae

Strain Designation: SKLS-A10

Culture col. no.: DSM 106441, NCAIM B. 02634

Strain history: <- A. Táncsics, Szent Istvàn University, Gödöllö, Hungary; SKLS-A10 <- F. Révész, Szent István University

NCBI tax ID(s): 2038656 (species)

SI-ID 398935

DSM 106441

Section

Sphingobium aquiterrae SKLS-A10 is an aerobe, Gram-negative, motile bacterium that forms circular colonies and was isolated from BTEX contaminated groundwater.

colony-forming
Gram-negative
motile
rod-shaped
aerobe
16S sequence
Bacteria

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Pseudomonadota*
	Class	*Alphaproteobacteria*
	Order	*Sphingomonadales*
	Family	*Sphingomonadaceae*
	Genus	*Sphingobium*
	Species	**Sphingobium aquiterrae**
	Full Scientific Name (LPSN)	Sphingobium aquiterrae Révész et al. 2018

Information on morphological and physiological properties Morphology

[Ref.: #66634]	Gram stain	negative

[Ref.: #66634]	Cell shape	rod-shaped

[Ref.: #66634]	Motility	yes

[Ref.: #66634]	Flagellum arrangement	monotrichous, polar

[Ref.: #66634]	Colony color	pale yellow
[Ref.: #66634]	Colony shape	circular
[Ref.: #66634]	Incubation period	3-4 days
[Ref.: #66634]	Cultivation medium used	R2A medium

Multimedia content

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Caption:

DSM 106441 on medium 830 at 28 °C

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Intellectual property rights:

Information on culture and growth conditions Culture and growth conditions

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Culture medium

Reasoner's 2A agar (R2A)

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Culture medium growth

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Culture medium

R2A MEDIUM (DSMZ Medium 830)

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Culture medium growth

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Culture medium link

Medium recipe at MediaDive

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Culture medium composition

expand / minimize

	Temperatures	Kind of temperature		Temperature
[Ref.: #66496]			growth	28 °C
[Ref.: #66634]			growth	4-40 °C

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Temperature range

mesophilic

	pH	Kind of pH		pH
[Ref.: #66634]			growth	5-11

Information on physiology and metabolism Physiology and metabolism

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Oxygen tolerance

aerobe

	Halophily	Salt	Tested relation		Salt conc.
[Ref.: #66634]		NaCl		growth	0	%(w/v)

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #66634]	adipate ChEBI	-	assimilation
[Ref.: #68369]	adipate ChEBI	-	assimilation	* from API 20NE
[Ref.: #66634]	amygdalin ChEBI	+	builds acid from
[Ref.: #66634]	arbutin ChEBI	-	builds acid from
[Ref.: #68369]	arginine ChEBI	-	hydrolysis	* from API 20NE
[Ref.: #66634]	benzene ChEBI	-	degradation
[Ref.: #66634]	cellobiose ChEBI	-	builds acid from
[Ref.: #66634]	D-arabinose ChEBI	-	builds acid from
[Ref.: #66634]	D-arabitol ChEBI	-	builds acid from
[Ref.: #66634]	D-fructose ChEBI	-	builds acid from
[Ref.: #66634]	D-fucose ChEBI	-	builds acid from
[Ref.: #66634]	D-galactose ChEBI	-	builds acid from
[Ref.: #66634]	D-glucose ChEBI	+	assimilation
[Ref.: #66634]	D-glucose ChEBI	+	builds acid from
[Ref.: #68369]	D-glucose ChEBI	+	assimilation	* from API 20NE
[Ref.: #68369]	D-glucose ChEBI	-	fermentation	* from API 20NE
[Ref.: #66634]	D-lyxose ChEBI	+	builds acid from
[Ref.: #66634]	D-mannitol ChEBI	-	assimilation
[Ref.: #66634]	D-mannitol ChEBI	-	builds acid from
[Ref.: #68369]	D-mannitol ChEBI	-	assimilation	* from API 20NE
[Ref.: #66634]	D-mannose ChEBI	-	assimilation
[Ref.: #66634]	D-mannose ChEBI	-	builds acid from
[Ref.: #68369]	D-mannose ChEBI	-	assimilation	* from API 20NE
[Ref.: #66634]	D-ribose ChEBI	-	builds acid from
[Ref.: #66634]	D-sorbitol ChEBI	-	builds acid from
[Ref.: #66634]	D-tagatose ChEBI	-	builds acid from
[Ref.: #66634]	D-xylose ChEBI	+	builds acid from
[Ref.: #66634]	decanoate ChEBI	-	assimilation
[Ref.: #68369]	decanoate ChEBI	-	assimilation	* from API 20NE
[Ref.: #66634]	erythritol ChEBI	-	builds acid from
[Ref.: #68369]	esculin ChEBI	+	hydrolysis	* from API 20NE
[Ref.: #66634]	esculin ferric citrate	+	builds acid from
[Ref.: #66634]	ethylbenzene ChEBI	-	degradation
[Ref.: #66634]	galactitol ChEBI	-	builds acid from
[Ref.: #68369]	gelatin ChEBI	-	hydrolysis	* from API 20NE
[Ref.: #66634]	gentiobiose ChEBI	+	builds acid from
[Ref.: #68369]	gluconate ChEBI	+	assimilation	* from API 20NE
[Ref.: #66634]	glycerol ChEBI	-	builds acid from
[Ref.: #66634]	glycogen ChEBI	-	builds acid from
[Ref.: #66634]	inulin ChEBI	-	builds acid from
[Ref.: #66634]	L-arabinose ChEBI	-	assimilation
[Ref.: #66634]	L-arabinose ChEBI	+	builds acid from
[Ref.: #68369]	L-arabinose ChEBI	-	assimilation	* from API 20NE
[Ref.: #66634]	L-arabitol ChEBI	-	builds acid from
[Ref.: #66634]	L-fucose ChEBI	-	builds acid from
[Ref.: #66634]	L-rhamnose ChEBI	+	builds acid from
[Ref.: #66634]	L-sorbose ChEBI	-	builds acid from
[Ref.: #66634]	L-xylose ChEBI	-	builds acid from
[Ref.: #66634]	lactose ChEBI	-	builds acid from
[Ref.: #66634]	m-xylene ChEBI	+	degradation
[Ref.: #66634]	malate ChEBI	-	assimilation
[Ref.: #68369]	malate ChEBI	-	assimilation	* from API 20NE
[Ref.: #66634]	maltose ChEBI	-	assimilation
[Ref.: #66634]	maltose ChEBI	-	builds acid from
[Ref.: #68369]	maltose ChEBI	-	assimilation	* from API 20NE
[Ref.: #66634]	melezitose ChEBI	-	builds acid from
[Ref.: #66634]	melibiose ChEBI	-	builds acid from
[Ref.: #66634]	methyl alpha-D-glucopyranoside ChEBI	-	builds acid from
[Ref.: #66634]	methyl alpha-D-mannoside ChEBI	-	builds acid from
[Ref.: #66634]	methyl beta-D-xylopyranoside ChEBI	-	builds acid from
[Ref.: #66634]	myo-inositol ChEBI	-	builds acid from
[Ref.: #66634]	N-acetylglucosamine ChEBI	-	assimilation
[Ref.: #66634]	N-acetylglucosamine ChEBI	-	builds acid from
[Ref.: #68369]	N-acetylglucosamine ChEBI	-	assimilation	* from API 20NE
[Ref.: #68369]	nitrate ChEBI	-	reduction	* from API 20NE
[Ref.: #66634]	p-xylene ChEBI	+	degradation
[Ref.: #66634]	phenylacetate ChEBI	-	assimilation
[Ref.: #66634]	potassium 2-dehydro-D-gluconate	-	builds acid from
[Ref.: #66634]	potassium 5-dehydro-D-gluconate	-	builds acid from
[Ref.: #66634]	potassium gluconate ChEBI	-	assimilation
[Ref.: #66634]	potassium gluconate ChEBI	-	builds acid from
[Ref.: #66634]	raffinose ChEBI	-	builds acid from
[Ref.: #66634]	ribitol ChEBI	-	builds acid from
[Ref.: #66634]	salicin ChEBI	-	builds acid from
[Ref.: #66634]	sodium citrate ChEBI	-	assimilation
[Ref.: #66634]	starch ChEBI	-	builds acid from
[Ref.: #66634]	sucrose ChEBI	-	builds acid from
[Ref.: #66634]	toluene ChEBI	+	degradation
[Ref.: #66634]	trehalose ChEBI	-	builds acid from
[Ref.: #68369]	tryptophan ChEBI	-	energy source	* from API 20NE
[Ref.: #66634]	turanose ChEBI	-	builds acid from
[Ref.: #68369]	urea ChEBI	-	hydrolysis	* from API 20NE
[Ref.: #66634]	xylitol ChEBI	-	builds acid from
	Only first 10 entries are displayed. Click here to see all.

	Metabolite production	Metabolite	Production
[Ref.: #68369]		indole ChEBI	no	* from API 20NE

	Physiological tests	Metabolite	Indole test
[Ref.: #68369]		indole ChEBI	-	* from API 20NE

	Enzyme	Enzyme activity	EC number
[Ref.: #66634]	acid phosphatase	+	3.1.3.2
[Ref.: #66634]	alkaline phosphatase	+	3.1.3.1
[Ref.: #66634]	alpha-chymotrypsin	+	3.4.21.1
[Ref.: #68369]	arginine dihydrolase	-	3.5.3.6	* from API 20NE
[Ref.: #66634]	beta-glucosidase	+	3.2.1.21
[Ref.: #68369]	beta-glucosidase	+	3.2.1.21	* from API 20NE
[Ref.: #66634]	cystine arylamidase	+	3.4.11.3
[Ref.: #68369]	cytochrome oxidase	+	1.9.3.1	* from API 20NE
[Ref.: #66634]	esterase (C 4)	+
[Ref.: #66634]	esterase Lipase (C 8)	+
[Ref.: #68369]	gelatinase	-		* from API 20NE
[Ref.: #66634]	leucine arylamidase	+	3.4.11.1
[Ref.: #66634]	naphthol-AS-BI-phosphohydrolase	+
[Ref.: #68369]	urease	-	3.5.1.5	* from API 20NE
[Ref.: #66634]	valine arylamidase	+
	Only first 10 entries are displayed. Click here to see all.

Fatty acid profile

Metadata FA analysis

Reference

[Ref.: #66634]

Fatty acid	Percentage
C18:1 w7c	55.7
C14:0 2OH	12.8
C16:1 w7c/iso C15:0 2OH	9
C17:1 w6c	6.6
C16:0	6.3
C18:1 w7c 11-methyl	1.9
C15:0 2OH	1.5
C16:1 w5c	1.5
C18:1 w5c	0.8

API® 20NE:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Reduction of nitratesNO3	Indole productionTRP	Fermentation of D-glucoseGLU_ Ferm	Arginine dihydrolaseADH (Arg)	Urease/urea hydrolysisURE	Esculin hydrolysis/beta-GlucosidaseESC	Gelatinase/gelatin hydrolysisGEL	beta-GalactosidasePNPG	Assimilation of D-glucoseGLU_ Assim	Assimilation of L-arabinoseARA	Assimilation of D-mannoseMNE	Assimilation of D-mannitolMAN	Assimilation of N-acetyl-glucosamineNAG	Assimilation of maltoseMAL	Assimilation of gluconateGNT	Assimilation of capric acidCAP	Assimilation adipic acidADI	Assimilation of malic acidMLT	Assimilation of citric acidCIT	Assimilation of phenyl-acetic acidPAC	Cytochrome oxidaseOX
[Ref.: #66496]	25084	-	-	-	-	-	+	-	-	+	-	-	-	-	-	+	-	-	-	-	-	+
[Ref.: #66496]	24658	-	-	-	-	-	+	-	-	+	-	-	-	-	-	+	-	-	-	-	-	+

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #66496]	Sample type/isolated from	BTEX contaminated groundwater
[Ref.: #66496]	Geographic location (country and/or sea, region)	Siklós (45° 51' 25.8'' N, 18° 17' 32.3'' E)
[Ref.: #66496]	Country	Hungary
[Ref.: #66496]	Country ISO 3 Code	HUN
[Ref.: #66496]	Continent	Europe

[Ref.: #66634]	Sample type/isolated from	groundwater sample (ca. 3m depth)
[Ref.: #66634]	Geographic location (country and/or sea, region)	Siklós BTEX contaminated site
[Ref.: #66634]	Country	Hungary
[Ref.: #66634]	Country ISO 3 Code	HUN
[Ref.: #66634]	Continent	Europe
[Ref.: #66634]	Geographic location	45.8571°/18.2923°
[Ref.: #66634]	Enrichment culture	R2A
[Ref.: #66634]	Enrichment culture duration	5 days
[Ref.: #66634]	Enrichment culture temperature	15 ̊C
[Ref.: #66634]	Isolation/enrichment procedure	a groundwater sample was serially diluted with 0.9 % (w/v) saline solution, spread onReasoner's 2A agar and incubated at 15°C for 5 days for isolation of bacterial strains
* marker position based on {}

Isolation sources categories

#Environmental	#Aquatic	#Groundwater
#Engineered	#Contamination	-

What are isolation sources categories?

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #66634]	Sphingobium sp. SKLS-A10 16S ribosomal RNA gene, partial sequence	MF980915	1409	ENA	2038656 tax ID

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GC-content

65.9 mol%

high performance liquid chromatography (HPLC)

Availability in culture collections External links

[Ref.: #66496]

Culture collection no.

DSM 106441, NCAIM B. 02634

[Ref.: #112120]

SI-ID 398935

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Sphingobium aquiterrae sp. nov., a toluene, meta- and para-xylene-degrading bacterium isolated from petroleum hydrocarbon-contaminated groundwater.	Revesz F, Toth EM, Kriszt B, Boka K, Benedek T, Sarkany O, Nagy Z, Tancsics A	Int J Syst Evol Microbiol	10.1099/ijsem.0.002898	2018	*

References

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#66496

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 106441

#66634

Fruzsina Revesz, Erika M. Toth, Balazs Kriszt, Karoly Boka, Tibor Benedek, Orsolya Sarkany, Zsuzsa Nagy, Andras Tancsics: Sphingobium aquiterrae sp. nov., a toluene, meta- and para-xylene-degrading bacterium isolated from petroleum hydrocarbon-contaminated groundwater. IJSEM 68: 2807 - 2812 2018 ( DOI 10.1099/ijsem.0.002898 , PubMed 29975186 )

#68369

Automatically annotated from API 20NE .

#112120

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID398935.1 )

* These data were automatically processed and therefore are not curated

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