Name: Actinomadura logoneensis NEAU-G17
Creator: BacDive
License: https://creativecommons.org/licenses/by/4.0/

Strain identifier

BacDive ID: 159574

Type strain:

Species: Actinomadura logoneensis

Strain Designation: NEAU-G17

Culture col. no.: DSM 105122, CGMCC 4.7411

Strain history: <- J. Zhao; Northeast Agricultural University, Harbin, P.R. CHINA; NEAU-G17<-J. Zhao; School of Life Science, Northeast Agricultural University

NCBI tax ID(s): 2293572 (species)

SI-ID 402472

DSM 105122

Special Collections

Section

When using BacDive for research please cite our paper

Actinomadura logoneensis NEAU-G17 is an aerobe, spore-forming, mesophilic bacterium that was isolated from soil.

spore-forming
aerobe
mesophilic
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	09-12-2024 (DD-MM-YYYY)
	Domain	*Bacteria*
	Phylum	*Actinomycetota*
	Class	*Actinomycetes*
	Order	*Streptosporangiales*
	Family	*Thermomonosporaceae*
	Genus	*Actinomadura*
	Species	**Actinomadura logoneensis**
	Full Scientific Name (LPSN)	Actinomadura logoneensis Shi et al. 2019

Information on morphological and physiological properties Morphology

[Ref.: #125439]	Gram stain	positive Confidence in %99.9

[Ref.: #69284]	Colony color	Beige (1001), Light ivory (1015)
[Ref.: #69284]	Cultivation medium used	ISP 2

[Ref.: #69284]	Colony color	Beige (1001), Light ivory (1015)
[Ref.: #69284]	Cultivation medium used	suter with tyrosine

[Ref.: #69284]	Colony color	Beige (1001)
[Ref.: #69284]	Cultivation medium used	ISP 7

[Ref.: #68183]	Colony color	yellow

[Ref.: #69284]	Colony color	Light ivory (1015)
[Ref.: #69284]	Cultivation medium used	ISP 3

[Ref.: #69284]	Colony color	Light ivory (1015)
[Ref.: #69284]	Cultivation medium used	suter without tyrosine

[Ref.: #69284]	Colony color	Ochre yellow (1024)
[Ref.: #69284]	Cultivation medium used	ISP 6

[Ref.: #69284]	Colony color	Oyster white (1013)
[Ref.: #69284]	Cultivation medium used	ISP 4

[Ref.: #69284]	Colony color	Oyster white (1013)
[Ref.: #69284]	Cultivation medium used	ISP 5

[Ref.: #69284]	Pigment production	no
[Ref.: #69284]	Pigment name	Melanin
[Ref.: #69284]	Pigment production	yes
[Ref.: #69284]	Pigmentation color	Sand yellow (1002), ivory (1014)
[Ref.: #69284]	Pigment name	soluble pigment

[Ref.: #68183]	Multicellular complex forming ability	yes
[Ref.: #68183]	Multicellular complex color	depending on medium aerial mycelium: greenish yellow (ISP1, ISP2, ISP7, Czapek's agar), yellow (ISP3, ISP4, ISP6, Bennett's agar) and orange yellow (ISP5)
[Ref.: #68183]	Medium Name (multicellularity)	ISP1, ISP2, ISP3, ISP4, ISP5, ISP6, ISP7, Bennett's agar, Czapek's agar
[Ref.: #68183]	Multicellular complex further description	well-developed, branched substrate hyphae and aerial mycelium that differentiates into flexuous spore chains consisted of cylindrical spores

[Ref.: #69284]	Multicellular complex forming ability	no
[Ref.: #69284]	Multicellular complex name	Aerial mycelium
[Ref.: #69284]	Medium Name (multicellularity)	ISP 2

[Ref.: #69284]	Multicellular complex forming ability	no
[Ref.: #69284]	Multicellular complex name	Aerial mycelium
[Ref.: #69284]	Medium Name (multicellularity)	ISP 3

[Ref.: #69284]	Multicellular complex forming ability	no
[Ref.: #69284]	Multicellular complex name	Aerial mycelium
[Ref.: #69284]	Medium Name (multicellularity)	ISP 4

[Ref.: #69284]	Multicellular complex forming ability	no
[Ref.: #69284]	Multicellular complex name	Aerial mycelium
[Ref.: #69284]	Medium Name (multicellularity)	ISP 5

[Ref.: #69284]	Multicellular complex forming ability	no
[Ref.: #69284]	Multicellular complex name	Aerial mycelium
[Ref.: #69284]	Medium Name (multicellularity)	ISP 6

[Ref.: #69284]	Multicellular complex forming ability	no
[Ref.: #69284]	Multicellular complex name	Aerial mycelium
[Ref.: #69284]	Medium Name (multicellularity)	ISP 7

[Ref.: #69284]	Multicellular complex forming ability	no
[Ref.: #69284]	Multicellular complex name	Aerial mycelium
[Ref.: #69284]	Medium Name (multicellularity)	suter with tyrosine

[Ref.: #69284]	Multicellular complex forming ability	no
[Ref.: #69284]	Multicellular complex name	Aerial mycelium
[Ref.: #69284]	Medium Name (multicellularity)	suter without tyrosine

Multimedia content

[Ref.: #66400]

Caption:

Medium 215 28°C

[Ref.: #66400]

Intellectual property rights:

Information on culture and growth conditions Culture and growth conditions

[Ref.: #66400]

Culture medium

BHI MEDIUM (DSMZ Medium 215)

[Ref.: #66400]

Culture medium growth

[Ref.: #66400]

Culture medium link

Medium recipe at MediaDive

[Ref.: #66400]

Culture medium composition

expand / minimize

[Ref.: #66400]

Culture medium

GPHF-MEDIUM (DSMZ Medium 553)

[Ref.: #66400]

Culture medium growth

[Ref.: #66400]

Culture medium link

Medium recipe at MediaDive

[Ref.: #66400]

Culture medium composition

expand / minimize

[Ref.: #66400]

Culture medium

N-Z-AMINE-MEDIUM (DSMZ Medium 554)

[Ref.: #66400]

Culture medium growth

[Ref.: #66400]

Culture medium link

Medium recipe provided by DSMZ

		Temperature
[Ref.: #66400]	growth	28 °C
[Ref.: #68183]	growth	25-42 °C
[Ref.: #68183]	optimum	37 °C

	pH	Kind of pH		pH
[Ref.: #68183]			optimum	7
[Ref.: #68183]			growth	5.0-10.0

Information on physiology and metabolism Physiology and metabolism

[Ref.: #68183]	Oxygen tolerance	aerobe
[Ref.: #125439]	Oxygen tolerance	obligate aerobe Confidence in %99.4

[Ref.: #68183]	Spore description	flexuous spore chains consisted of cylindrical spores (0.5-0.7 µm x 0.8-1.0 µm)
[Ref.: #68183]	Type of spore	spore
[Ref.: #68183]	Ability of spore formation	yes
[Ref.: #125439]	Ability of spore formation	yes Confidence in %95.4

	Halophily	Salt	Tested relation		Salt conc.
[Ref.: #68183]		NaCl		growth	0-4.0	%(w/v)
[Ref.: #69284]		NaCl		growth	0-5	%

[Ref.: #68183]

Observation

forms well-developed, branched substrate hyphae and aerial mycelium that differentiates into flexuous spore chains consisted of cylindrical spores

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #69284]	arabinose ChEBI	+	growth
[Ref.: #68183]	cellulose ChEBI	-	assimilation
[Ref.: #69284]	cellulose ChEBI	+	growth
[Ref.: #68183]	creatine ChEBI	-	nitrogen source
[Ref.: #68183]	D-fructose ChEBI	+	carbon source
[Ref.: #68183]	D-galactose ChEBI	+	carbon source
[Ref.: #68183]	D-glucose ChEBI	+	carbon source
[Ref.: #68379]	D-glucose ChEBI	-	fermentation	* from API Coryne
[Ref.: #68183]	D-mannitol ChEBI	-	carbon source
[Ref.: #68379]	D-mannitol ChEBI	-	fermentation	* from API Coryne
[Ref.: #68183]	D-mannose ChEBI	+	carbon source
[Ref.: #68183]	D-ribose ChEBI	-	carbon source
[Ref.: #68379]	D-ribose ChEBI	-	fermentation	* from API Coryne
[Ref.: #68183]	D-sorbitol ChEBI	-	carbon source
[Ref.: #68183]	D-xylose ChEBI	-	carbon source
[Ref.: #68379]	D-xylose ChEBI	-	fermentation	* from API Coryne
[Ref.: #68183]	esculin ChEBI	-	assimilation
[Ref.: #68379]	esculin ChEBI	-	hydrolysis	* from API Coryne
[Ref.: #69284]	fructose ChEBI	+	growth
[Ref.: #68183]	galactitol ChEBI	-	carbon source
[Ref.: #68183]	gelatin ChEBI	-	hydrolysis
[Ref.: #68379]	gelatin ChEBI	-	hydrolysis	* from API Coryne
[Ref.: #69284]	glucose ChEBI	+	growth
[Ref.: #68183]	glycine ChEBI	+	nitrogen source
[Ref.: #68379]	glycogen ChEBI	-	fermentation	* from API Coryne
[Ref.: #69284]	inositol ChEBI	+	growth
[Ref.: #68183]	L-alanine ChEBI	+	nitrogen source
[Ref.: #68183]	L-arabinose ChEBI	+	carbon source
[Ref.: #68183]	L-arginine-2-naphthylamide ChEBI	+	nitrogen source
[Ref.: #68183]	L-asparagine ChEBI	+	nitrogen source
[Ref.: #68183]	L-aspartate ChEBI	+	nitrogen source
[Ref.: #68183]	L-glutamate ChEBI	-	nitrogen source
[Ref.: #68183]	L-glutamine ChEBI	+	nitrogen source
[Ref.: #68183]	L-proline ChEBI	+	nitrogen source
[Ref.: #68183]	L-rhamnose ChEBI	+	carbon source
[Ref.: #68183]	L-serine ChEBI	+	nitrogen source
[Ref.: #68183]	L-threonine ChEBI	-	nitrogen source
[Ref.: #68183]	L-tyrosine ChEBI	-	nitrogen source
[Ref.: #68183]	lactose ChEBI	+	carbon source
[Ref.: #68379]	lactose ChEBI	-	fermentation	* from API Coryne
[Ref.: #68183]	maltose ChEBI	+	carbon source
[Ref.: #68379]	maltose ChEBI	-	fermentation	* from API Coryne
[Ref.: #69284]	mannose ChEBI	+	growth
[Ref.: #68183]	milk	-	assimilation
[Ref.: #68183]	myo-inositol ChEBI	+	carbon source
[Ref.: #68183]	nitrate ChEBI	-	reduction
[Ref.: #68379]	nitrate ChEBI	+	reduction	* from API Coryne
[Ref.: #68183]	raffinose ChEBI	+	carbon source
[Ref.: #69284]	raffinose ChEBI	+	growth
[Ref.: #69284]	rhamnose ChEBI	+	growth
[Ref.: #68183]	starch ChEBI	-	hydrolysis
[Ref.: #68183]	sucrose ChEBI	+	carbon source
[Ref.: #69284]	sucrose ChEBI	+	growth
[Ref.: #68379]	sucrose ChEBI	-	fermentation	* from API Coryne
[Ref.: #68183]	tween 20 ChEBI	-	hydrolysis
[Ref.: #68183]	tween 40 ChEBI	-	hydrolysis
[Ref.: #68183]	tween 80 ChEBI	-	hydrolysis
[Ref.: #68379]	urea ChEBI	-	hydrolysis	* from API Coryne
[Ref.: #69284]	xylose ChEBI	+	growth
	Only first 10 entries are displayed. Click here to see all.

	Metabolite production	Metabolite	Production
[Ref.: #68183]		hydrogen sulfide ChEBI	no

	Enzyme	Enzyme activity	EC number
[Ref.: #68382]	acid phosphatase	+	3.1.3.2	* from API zym
[Ref.: #68379]	alkaline phosphatase	-	3.1.3.1	* from API Coryne
[Ref.: #68382]	alpha-chymotrypsin	-	3.4.21.1	* from API zym
[Ref.: #68382]	alpha-fucosidase	-	3.2.1.51	* from API zym
[Ref.: #68382]	alpha-galactosidase	-	3.2.1.22	* from API zym
[Ref.: #68382]	alpha-glucosidase	+	3.2.1.20	* from API zym
[Ref.: #68379]	alpha-glucosidase	-	3.2.1.20	* from API Coryne
[Ref.: #68382]	alpha-mannosidase	-	3.2.1.24	* from API zym
[Ref.: #68379]	beta-galactosidase	-	3.2.1.23	* from API Coryne
[Ref.: #68379]	beta-glucosidase	-	3.2.1.21	* from API Coryne, API zym
[Ref.: #68379]	beta-glucuronidase	-	3.2.1.31	* from API Coryne, API zym
[Ref.: #68183]	catalase	-	1.11.1.6
[Ref.: #68382]	esterase (C 4)	+		* from API zym
[Ref.: #68382]	esterase lipase (C 8)	+		* from API zym
[Ref.: #68379]	gelatinase	-		* from API Coryne
[Ref.: #68382]	leucine arylamidase	+	3.4.11.1	* from API zym
[Ref.: #68379]	N-acetyl-beta-glucosaminidase	+	3.2.1.52	* from API Coryne, API zym
[Ref.: #68382]	naphthol-AS-BI-phosphohydrolase	+		* from API zym
[Ref.: #68379]	pyrazinamidase	-	3.5.1.B15	* from API Coryne
[Ref.: #68379]	pyrrolidonyl arylamidase	-	3.4.19.3	* from API Coryne
[Ref.: #68382]	trypsin	-	3.4.21.4	* from API zym
[Ref.: #68183]	urease	-	3.5.1.5
[Ref.: #68379]	urease	-	3.5.1.5	* from API Coryne
[Ref.: #68382]	valine arylamidase	+		* from API zym
	Only first 10 entries are displayed. Click here to see all.

Fatty acid profile

Metadata FA analysis

Reference

[Ref.: #68183]

Fatty acid	Percentage
C16:0	33.2
C18:1 w9c	13.8
C16:0 iso	10.5
C17:0	10.1
C19:0 10-methly	9.7
C18:0	9.7
C17:1 w7c	5.5
C18:0 10-methly	3.3
C15:0 iso	2.8
C16:1 w7c	1.4

API® Coryne:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Reduction of nitrateNIT	PyrazinamidasePYZ	Pyrrolidonyl arylamidasePYRA	Alkaline phosphatasePAL	beta-Glucuronidasebeta GUR	beta-Galactosidasebeta GAL	alpha-Glucosidasealpha GLU	N-Acetyl-beta-Glucosidasebeta NAG	Esculin hydrolysis/beta-GlucosidaseESC	Urease/urea hydrolysisURE	Gelatinase/gelatin hydrolysisGEL	Control fermentationControl	Fermentation of D-glucoseGLU	Fermentation of D-riboseRIB	Fermentation of D-xyloseXYL	Fermentation of D-mannitolMAN	Fermentation of maltoseMAL	Fermentation of lactoseLAC	Fermentation of sucroseSAC	Fermentation of glycogenGLYG	CatalaseCAT
[Ref.: #69284]	3778	+	-	-	-	-	-	-	+	-	-	-	not determinedn.d.	-	-	-	-	-	-	-	-	not determinedn.d.

API® zym:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Control	2-naphthyl phosphateAlkaline phosphatase	2-naphthyl butyrateEsterase (C 4)	2-naphtyl caprylateEsterase Lipase (C 8)	2-naphthyl myristateLipase (C 14)	L-leucyl-2-naphthylamideLeucine arylamidase	L-valyl-2-naphthylamideValine arylamidase	L-cystyl-2-naphthylamideCystine arylamidase	N-benzoyl-DL-arginine-2-naphthylamideTrypsin	N-glutaryl-phenylalanine-2-naphthylamidealpha- Chymotrypsin	2-naphthyl phosphateAcid phosphatase	Naphthol-AS-BI-phosphateNaphthol-AS-BI-phosphohydrolase	6-Br-2-naphthyl-alphaD-galactpyranosidealpha- Galactosidase	2-naphthyl-betaD-galactpyranosidebeta- Galactosidase	Naphthol-AS-BI-betaD-glucuronidebeta- Glucuronidase	2-naphthyl-alphaD-glucopyranosidealpha- Glucosidase	6-Br-2-naphthyl-betaD-glucopyranosidebeta- Glucosidase	1-naphthyl-N-acetyl-betaD-glucosaminideN-acetyl-beta- glucosaminidase	6-Br-2-naphthyl-alphaD-mannopyranosidealpha- Mannosidase	2-naphthyl-alphaL-fucopyranosidealpha- Fucosidase
[Ref.: #69284]	5962	not determinedn.d.	+/-	+	+	+/-	+	+	+/-	-	-	+	+	-	+/-	-	+	-	+	-	-

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #66400]	Sample type/isolated from	soil
[Ref.: #66400]	Geographic location (country and/or sea, region)	Logone riverbank, N’Djamena (12° 06' N, 15° 03' E)
[Ref.: #66400]	Country	Chad
[Ref.: #66400]	Country ISO 3 Code	TCD
[Ref.: #66400]	Continent	Africa
[Ref.: #66400]	Geographic location	12.1000°/15.0500°

[Ref.: #68183]	Sample type/isolated from	muddy soil collected from Logone riverbank
[Ref.: #68183]	Geographic location (country and/or sea, region)	N'Djamena
[Ref.: #68183]	Country	Chad
[Ref.: #68183]	Country ISO 3 Code	TCD
[Ref.: #68183]	Continent	Africa
[Ref.: #68183]	Geographic location	12.1000°/15.0500°
[Ref.: #68183]	Enrichment culture	sodium succinate-asparagine agar
[Ref.: #68183]	Enrichment culture duration	21 days
[Ref.: #68183]	Enrichment culture temperature	28 ̊C
* marker position based on {}

Isolation sources categories

#Environmental

#Terrestrial

#Soil

What are isolation sources categories?

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #66400]	Actinomadura logoneensis strain NEAU-G17 16S ribosomal RNA gene, partial sequence	MG189967	1520	GenBank ENA	1989 tax ID

Genome sequence information:

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #68183]	Actinomadura logoneensis NEAU-G17	GCA_003428695	contig	GenBank ENA	2293572 tax ID
[Ref.: #66792]	Actinomadura logoneensis NEAU-G17	2841009013	draft	JGI IMG/M	2293572 tax ID	*

[Ref.: #66400]	GC-content	73.1 mol%	sequence analysis
[Ref.: #68183]	GC-content	73.1 mol%	genome sequence analysis

Data predicted using genome information as a basis Genome-based predictions

	Model	Trait	Prediction	Confidence in %
	Predictions from Deep Learning for Genome Sequence Data. R package version 0.3.1 based on: Actinomadura logoneensis NEAU-G17 NCBI: GCA_003428695
[Ref.: #125439]	BacteriaNet	spore_formationⓘ	yes	95.4
[Ref.: #125439]	BacteriaNet	motilityⓘ	no	89.1
[Ref.: #125439]	BacteriaNet	gram_stainⓘ	positive	99.9
[Ref.: #125439]	BacteriaNet	oxygen_toleranceⓘ	obligate aerobe	99.4

Availability in culture collections External links

[Ref.: #66400]

Culture collection no.

DSM 105122, CGMCC 4.7411

[Ref.: #112050]

SI-ID 402472

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Actinomadura harenae sp. nov., a novel actinomycete isolated from sea sand in Sanya.	Hu J, Han C, Yu B, Zhao J, Guo X, Shen Y, Wang X, Xiang W	Int J Syst Evol Microbiol	10.1099/ijsem.0.003819	2020	*
Phylogeny	Actinomadura logoneensis sp. nov., a novel actinomycete isolated from the soil.	Shi L, Han L, Guo X, Zhao J, Wang J, Wang H, Jiang S, Xiang W, Wang X	Int J Syst Evol Microbiol	10.1099/ijsem.0.003579	2019	*

References

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#66400

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 105122

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#68183

Linlin Shi, Liyuan Han, Xiaowei Guo, Junwei Zhao, Jiabin Wang, Han Wang, Shanwen Jiang, Wensheng Xiang and Xiangjing Wang: Actinomadura logoneensis sp. nov., a novel actinomycete isolated from the soil. IJSEM 69: 2914 - 2920 2019 ( DOI 10.1099/ijsem.0.003579 )

#68379

Automatically annotated from API Coryne .

#68382

Automatically annotated from API zym .

#69284

Wink, J.: Compendium of Actinobacteria. HZI-Helmholtz-Centre for Infection Research, Braunschweig .

#112050

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID402472.1 )

#125439

Philipp Münch, René Mreches, Martin Binder, Hüseyin Anil Gündüz, Xiao-Yin To, Alice McHardy: deepG: Deep Learning for Genome Sequence Data. R package version 0.3.1 .

* These data were automatically processed and therefore are not curated

Change proposal

You found an error in BacDive? Please tell us about it!

Note that changes will be reviewed and judged. If your changes are legitimate, changes will occur within the next BacDive update. Only proposed changes supported by the according reference will be reviewed. The BacDive team reserves the right to reject proposed changes.

Search for species Actinomadura logoneensis in external resources:
SILVA
BRENDA
PANGAEA
GBIF