Name: Actinomadura logoneensis NEAU-G17
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 159574

Type strain:

Species: Actinomadura logoneensis

Strain Designation: NEAU-G17

Culture col. no.: DSM 105122, CGMCC 4.7411

Strain history: <- J. Zhao; Northeast Agricultural University, Harbin, P.R. CHINA; NEAU-G17<-J. Zhao; School of Life Science, Northeast Agricultural University

NCBI tax ID(s): 2293572 (species)

SI-ID 402472

DSM 105122

Section

Actinomadura logoneensis NEAU-G17 is an aerobe, spore-forming, mesophilic bacterium that was isolated from soil.

spore-forming
aerobe
mesophilic
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Actinomycetota*
	Class	*Actinomycetes*
	Order	*Streptosporangiales*
	Family	*Thermomonosporaceae*
	Genus	*Actinomadura*
	Species	**Actinomadura logoneensis**
	Full Scientific Name (LPSN)	Actinomadura logoneensis Shi et al. 2019

Information on morphological and physiological properties Morphology

[Ref.: #69284]	Colony color	Beige (1001), Light ivory (1015)
[Ref.: #69284]	Cultivation medium used	ISP 2

[Ref.: #69284]	Colony color	Beige (1001), Light ivory (1015)
[Ref.: #69284]	Cultivation medium used	suter with tyrosine

[Ref.: #69284]	Colony color	Beige (1001)
[Ref.: #69284]	Cultivation medium used	ISP 7

[Ref.: #68183]	Colony color	yellow

[Ref.: #69284]	Colony color	Light ivory (1015)
[Ref.: #69284]	Cultivation medium used	ISP 3

[Ref.: #69284]	Colony color	Light ivory (1015)
[Ref.: #69284]	Cultivation medium used	suter without tyrosine

[Ref.: #69284]	Colony color	Ochre yellow (1024)
[Ref.: #69284]	Cultivation medium used	ISP 6

[Ref.: #69284]	Colony color	Oyster white (1013)
[Ref.: #69284]	Cultivation medium used	ISP 4

[Ref.: #69284]	Colony color	Oyster white (1013)
[Ref.: #69284]	Cultivation medium used	ISP 5

[Ref.: #69284]	Pigment production	no
[Ref.: #69284]	Pigment name	Melanin
[Ref.: #69284]	Pigment production	yes
[Ref.: #69284]	Pigmentation color	Sand yellow (1002), ivory (1014)
[Ref.: #69284]	Pigment name	soluble pigment

[Ref.: #68183]	Multicellular complex forming ability	yes
[Ref.: #68183]	Multicellular complex color	depending on medium aerial mycelium: greenish yellow (ISP1, ISP2, ISP7, Czapek's agar), yellow (ISP3, ISP4, ISP6, Bennett's agar) and orange yellow (ISP5)
[Ref.: #68183]	Medium Name (multicellularity)	ISP1, ISP2, ISP3, ISP4, ISP5, ISP6, ISP7, Bennett's agar, Czapek's agar
[Ref.: #68183]	Multicellular complex further description	well-developed, branched substrate hyphae and aerial mycelium that differentiates into flexuous spore chains consisted of cylindrical spores

[Ref.: #69284]	Multicellular complex forming ability	no
[Ref.: #69284]	Multicellular complex name	Aerial mycelium
[Ref.: #69284]	Medium Name (multicellularity)	ISP 2

[Ref.: #69284]	Multicellular complex forming ability	no
[Ref.: #69284]	Multicellular complex name	Aerial mycelium
[Ref.: #69284]	Medium Name (multicellularity)	ISP 3

[Ref.: #69284]	Multicellular complex forming ability	no
[Ref.: #69284]	Multicellular complex name	Aerial mycelium
[Ref.: #69284]	Medium Name (multicellularity)	ISP 4

[Ref.: #69284]	Multicellular complex forming ability	no
[Ref.: #69284]	Multicellular complex name	Aerial mycelium
[Ref.: #69284]	Medium Name (multicellularity)	ISP 5

[Ref.: #69284]	Multicellular complex forming ability	no
[Ref.: #69284]	Multicellular complex name	Aerial mycelium
[Ref.: #69284]	Medium Name (multicellularity)	ISP 6

[Ref.: #69284]	Multicellular complex forming ability	no
[Ref.: #69284]	Multicellular complex name	Aerial mycelium
[Ref.: #69284]	Medium Name (multicellularity)	ISP 7

[Ref.: #69284]	Multicellular complex forming ability	no
[Ref.: #69284]	Multicellular complex name	Aerial mycelium
[Ref.: #69284]	Medium Name (multicellularity)	suter with tyrosine

[Ref.: #69284]	Multicellular complex forming ability	no
[Ref.: #69284]	Multicellular complex name	Aerial mycelium
[Ref.: #69284]	Medium Name (multicellularity)	suter without tyrosine

Multimedia content

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Caption:

Medium 215 28°C

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Intellectual property rights:

Information on culture and growth conditions Culture and growth conditions

[Ref.: #66400]

Culture medium

BHI MEDIUM (DSMZ Medium 215)

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Culture medium growth

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Culture medium link

Medium recipe at MediaDive

[Ref.: #66400]

Culture medium composition

expand / minimize

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Culture medium

GPHF-MEDIUM (DSMZ Medium 553)

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Culture medium growth

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Culture medium link

Medium recipe at MediaDive

[Ref.: #66400]

Culture medium composition

expand / minimize

		Temperature
[Ref.: #66400]	growth	28 °C
[Ref.: #68183]	growth	25-42 °C
[Ref.: #68183]	optimum	37 °C

[Ref.: #66400]	Temperature range	mesophilic
[Ref.: #68183]	Temperature range	mesophilic

	pH	Kind of pH		pH
[Ref.: #68183]			optimum	7
[Ref.: #68183]			growth	5.0-10.0

Information on physiology and metabolism Physiology and metabolism

[Ref.: #68183]

Oxygen tolerance

aerobe

[Ref.: #68183]	Spore description	flexuous spore chains consisted of cylindrical spores (0.5-0.7 µm x 0.8-1.0 µm)
[Ref.: #68183]	Type of spore	spore
[Ref.: #68183]	Ability of spore formation	yes

	Halophily	Salt	Tested relation		Salt conc.
[Ref.: #68183]		NaCl		growth	0-4.0	%(w/v)
[Ref.: #69284]		NaCl		growth	0-5	%

[Ref.: #68183]

Observation

forms well-developed, branched substrate hyphae and aerial mycelium that differentiates into flexuous spore chains consisted of cylindrical spores

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #69284]	arabinose ChEBI	+	growth
[Ref.: #68183]	cellulose ChEBI	-	assimilation
[Ref.: #69284]	cellulose ChEBI	+	growth
[Ref.: #68183]	creatine ChEBI	-	nitrogen source
[Ref.: #68183]	D-fructose ChEBI	+	carbon source
[Ref.: #68183]	D-galactose ChEBI	+	carbon source
[Ref.: #68183]	D-glucose ChEBI	+	carbon source
[Ref.: #68379]	D-glucose ChEBI	-	fermentation	* from API Coryne
[Ref.: #68183]	D-mannitol ChEBI	-	carbon source
[Ref.: #68379]	D-mannitol ChEBI	-	fermentation	* from API Coryne
[Ref.: #68183]	D-mannose ChEBI	+	carbon source
[Ref.: #68183]	D-ribose ChEBI	-	carbon source
[Ref.: #68379]	D-ribose ChEBI	-	fermentation	* from API Coryne
[Ref.: #68183]	D-sorbitol ChEBI	-	carbon source
[Ref.: #68183]	D-xylose ChEBI	-	carbon source
[Ref.: #68379]	D-xylose ChEBI	-	fermentation	* from API Coryne
[Ref.: #68183]	esculin ChEBI	-	assimilation
[Ref.: #68379]	esculin ChEBI	-	hydrolysis	* from API Coryne
[Ref.: #69284]	fructose ChEBI	+	growth
[Ref.: #68183]	galactitol ChEBI	-	carbon source
[Ref.: #68183]	gelatin ChEBI	-	hydrolysis
[Ref.: #68379]	gelatin ChEBI	-	hydrolysis	* from API Coryne
[Ref.: #69284]	glucose ChEBI	+	growth
[Ref.: #68183]	glycine ChEBI	+	nitrogen source
[Ref.: #68379]	glycogen ChEBI	-	fermentation	* from API Coryne
[Ref.: #69284]	inositol ChEBI	+	growth
[Ref.: #68183]	L-alanine ChEBI	+	nitrogen source
[Ref.: #68183]	L-arabinose ChEBI	+	carbon source
[Ref.: #68183]	L-arginine-2-naphthylamide ChEBI	+	nitrogen source
[Ref.: #68183]	L-asparagine ChEBI	+	nitrogen source
[Ref.: #68183]	L-aspartate ChEBI	+	nitrogen source
[Ref.: #68183]	L-glutamate ChEBI	-	nitrogen source
[Ref.: #68183]	L-glutamine ChEBI	+	nitrogen source
[Ref.: #68183]	L-proline ChEBI	+	nitrogen source
[Ref.: #68183]	L-rhamnose ChEBI	+	carbon source
[Ref.: #68183]	L-serine ChEBI	+	nitrogen source
[Ref.: #68183]	L-threonine ChEBI	-	nitrogen source
[Ref.: #68183]	L-tyrosine ChEBI	-	nitrogen source
[Ref.: #68183]	lactose ChEBI	+	carbon source
[Ref.: #68379]	lactose ChEBI	-	fermentation	* from API Coryne
[Ref.: #68183]	maltose ChEBI	+	carbon source
[Ref.: #68379]	maltose ChEBI	-	fermentation	* from API Coryne
[Ref.: #69284]	mannose ChEBI	+	growth
[Ref.: #68183]	milk	-	assimilation
[Ref.: #68183]	myo-inositol ChEBI	+	carbon source
[Ref.: #68183]	nitrate ChEBI	-	reduction
[Ref.: #68379]	nitrate ChEBI	+	reduction	* from API Coryne
[Ref.: #68183]	raffinose ChEBI	+	carbon source
[Ref.: #69284]	raffinose ChEBI	+	growth
[Ref.: #69284]	rhamnose ChEBI	+	growth
[Ref.: #68183]	starch ChEBI	-	hydrolysis
[Ref.: #68183]	sucrose ChEBI	+	carbon source
[Ref.: #69284]	sucrose ChEBI	+	growth
[Ref.: #68379]	sucrose ChEBI	-	fermentation	* from API Coryne
[Ref.: #68183]	tween 20 ChEBI	-	hydrolysis
[Ref.: #68183]	tween 40 ChEBI	-	hydrolysis
[Ref.: #68183]	tween 80 ChEBI	-	hydrolysis
[Ref.: #68379]	urea ChEBI	-	hydrolysis	* from API Coryne
[Ref.: #69284]	xylose ChEBI	+	growth
	Only first 10 entries are displayed. Click here to see all.

	Metabolite production	Metabolite	Production
[Ref.: #68183]		hydrogen sulfide ChEBI	no

	Enzyme	Enzyme activity	EC number
[Ref.: #68382]	acid phosphatase	+	3.1.3.2	* from API zym
[Ref.: #68379]	alkaline phosphatase	-	3.1.3.1	* from API Coryne
[Ref.: #68382]	alpha-chymotrypsin	-	3.4.21.1	* from API zym
[Ref.: #68382]	alpha-fucosidase	-	3.2.1.51	* from API zym
[Ref.: #68382]	alpha-galactosidase	-	3.2.1.22	* from API zym
[Ref.: #68382]	alpha-glucosidase	+	3.2.1.20	* from API zym
[Ref.: #68379]	alpha-glucosidase	-	3.2.1.20	* from API Coryne
[Ref.: #68382]	alpha-mannosidase	-	3.2.1.24	* from API zym
[Ref.: #68379]	beta-galactosidase	-	3.2.1.23	* from API Coryne
[Ref.: #68379]	beta-glucosidase	-	3.2.1.21	* from API Coryne, API zym
[Ref.: #68379]	beta-glucuronidase	-	3.2.1.31	* from API Coryne, API zym
[Ref.: #68183]	catalase	-	1.11.1.6
[Ref.: #68382]	esterase (C 4)	+		* from API zym
[Ref.: #68382]	esterase lipase (C 8)	+		* from API zym
[Ref.: #68379]	gelatinase	-		* from API Coryne
[Ref.: #68382]	leucine arylamidase	+	3.4.11.1	* from API zym
[Ref.: #68379]	N-acetyl-beta-glucosaminidase	+	3.2.1.52	* from API Coryne, API zym
[Ref.: #68382]	naphthol-AS-BI-phosphohydrolase	+		* from API zym
[Ref.: #68379]	pyrazinamidase	-	3.5.1.B15	* from API Coryne
[Ref.: #68379]	pyrrolidonyl arylamidase	-	3.4.19.3	* from API Coryne
[Ref.: #68382]	trypsin	-	3.4.21.4	* from API zym
[Ref.: #68183]	urease	-	3.5.1.5
[Ref.: #68379]	urease	-	3.5.1.5	* from API Coryne
[Ref.: #68382]	valine arylamidase	+		* from API zym
	Only first 10 entries are displayed. Click here to see all.

Fatty acid profile

Metadata FA analysis

Reference

[Ref.: #68183]

Fatty acid	Percentage
C16:0	33.2
C18:1 w9c	13.8
C16:0 iso	10.5
C17:0	10.1
C19:0 10-methly	9.7
C18:0	9.7
C17:1 w7c	5.5
C18:0 10-methly	3.3
C15:0 iso	2.8
C16:1 w7c	1.4

API® Coryne:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Reduction of nitrateNIT	PyrazinamidasePYZ	Pyrrolidonyl arylamidasePYRA	Alkaline phosphatasePAL	beta-Glucuronidasebeta GUR	beta-Galactosidasebeta GAL	alpha-Glucosidasealpha GLU	N-Acetyl-beta-Glucosidasebeta NAG	Esculin hydrolysis/beta-GlucosidaseESC	Urease/urea hydrolysisURE	Gelatinase/gelatin hydrolysisGEL	Control fermentationControl	Fermentation of D-glucoseGLU	Fermentation of D-riboseRIB	Fermentation of D-xyloseXYL	Fermentation of D-mannitolMAN	Fermentation of maltoseMAL	Fermentation of lactoseLAC	Fermentation of sucroseSAC	Fermentation of glycogenGLYG	CatalaseCAT
[Ref.: #69284]	3778	+	-	-	-	-	-	-	+	-	-	-	not determinedn.d.	-	-	-	-	-	-	-	-	not determinedn.d.

API® zym:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Control	2-naphthyl phosphateAlkaline phosphatase	2-naphthyl butyrateEsterase (C 4)	2-naphtyl caprylateEsterase Lipase (C 8)	2-naphthyl myristateLipase (C 14)	L-leucyl-2-naphthylamideLeucine arylamidase	L-valyl-2-naphthylamideValine arylamidase	L-cystyl-2-naphthylamideCystine arylamidase	N-benzoyl-DL-arginine-2-naphthylamideTrypsin	N-glutaryl-phenylalanine-2-naphthylamidealpha- Chymotrypsin	2-naphthyl phosphateAcid phosphatase	Naphthol-AS-BI-phosphateNaphthol-AS-BI-phosphohydrolase	6-Br-2-naphthyl-alphaD-galactpyranosidealpha- Galactosidase	2-naphthyl-betaD-galactpyranosidebeta- Galactosidase	Naphthol-AS-BI-betaD-glucuronidebeta- Glucuronidase	2-naphthyl-alphaD-glucopyranosidealpha- Glucosidase	6-Br-2-naphthyl-betaD-glucopyranosidebeta- Glucosidase	1-naphthyl-N-acetyl-betaD-glucosaminideN-acetyl-beta- glucosaminidase	6-Br-2-naphthyl-alphaD-mannopyranosidealpha- Mannosidase	2-naphthyl-alphaL-fucopyranosidealpha- Fucosidase
[Ref.: #69284]	5962	not determinedn.d.	+/-	+	+	+/-	+	+	+/-	-	-	+	+	-	+/-	-	+	-	+	-	-

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #66400]	Sample type/isolated from	soil
[Ref.: #66400]	Geographic location (country and/or sea, region)	Logone riverbank, N’Djamena (12° 06' N, 15° 03' E)
[Ref.: #66400]	Country	Chad
[Ref.: #66400]	Country ISO 3 Code	TCD
[Ref.: #66400]	Continent	Africa
[Ref.: #66400]	Geographic location	12.1000°/15.0500°

[Ref.: #68183]	Sample type/isolated from	muddy soil collected from Logone riverbank
[Ref.: #68183]	Geographic location (country and/or sea, region)	N'Djamena
[Ref.: #68183]	Country	Chad
[Ref.: #68183]	Country ISO 3 Code	TCD
[Ref.: #68183]	Continent	Africa
[Ref.: #68183]	Geographic location	12.1000°/15.0500°
[Ref.: #68183]	Enrichment culture	sodium succinate-asparagine agar
[Ref.: #68183]	Enrichment culture duration	21 days
[Ref.: #68183]	Enrichment culture temperature	28 ̊C
* marker position based on {}

Isolation sources categories

#Environmental

#Terrestrial

#Soil

What are isolation sources categories?

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #66400]	Actinomadura sp. strain NEAU-G17 16S ribosomal RNA gene, partial sequence	MG189967	1520	ENA	1989 tax ID

Genome sequence information:

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #68183]	Actinomadura logoneensis NEAU-G17	GCA_003428695	contig	GenBank	2293572 tax ID
[Ref.: #66792]	Actinomadura logoneensis NEAU-G17	2841009013	draft	JGI IMG/M	2293572 tax ID	*

[Ref.: #66400]	GC-content	73.1 mol%	sequence analysis
[Ref.: #68183]	GC-content	73.1 mol%	genome sequence analysis

Availability in culture collections External links

[Ref.: #66400]

Culture collection no.

DSM 105122, CGMCC 4.7411

[Ref.: #112050]

SI-ID 402472

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Actinomadura harenae sp. nov., a novel actinomycete isolated from sea sand in Sanya.	Hu J, Han C, Yu B, Zhao J, Guo X, Shen Y, Wang X, Xiang W	Int J Syst Evol Microbiol	10.1099/ijsem.0.003819	2020	*
Phylogeny	Actinomadura logoneensis sp. nov., a novel actinomycete isolated from the soil.	Shi L, Han L, Guo X, Zhao J, Wang J, Wang H, Jiang S, Xiang W, Wang X	Int J Syst Evol Microbiol	10.1099/ijsem.0.003579	2019	*

References

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#66400

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 105122

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#68183

Linlin Shi, Liyuan Han, Xiaowei Guo, Junwei Zhao, Jiabin Wang, Han Wang, Shanwen Jiang, Wensheng Xiang and Xiangjing Wang: Actinomadura logoneensis sp. nov., a novel actinomycete isolated from the soil. IJSEM 69: 2914 - 2920 2019 ( DOI 10.1099/ijsem.0.003579 )

#68379

Automatically annotated from API Coryne .

#68382

Automatically annotated from API zym .

#69284

Wink, J.: Compendium of Actinobacteria. HZI-Helmholtz-Centre for Infection Research, Braunschweig .

#112050

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID402472.1 )

* These data were automatically processed and therefore are not curated

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