Name: Acholeplasma equirhinis N93
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 159212

Type strain:

Species: Acholeplasma equirhinis

Strain Designation: N93

Culture col. no.: DSM 106692, NCTC 14351, ATCC TSD-139

Strain history: <- D. V. Volokhov, Dept. LMD, Center for Biologics Evaluation and Research; N93 <- R. M. Lemcke and D. V. Volokhov

NCBI tax ID(s): 555393 (species)

Section

Acholeplasma equirhinis N93 is a facultative anaerobe, mesophilic bacterium that forms fried-egg-shaped colonies and was isolated from nasopharyngeal swab of a horse.

colony-forming
facultative anaerobe
mesophilic
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	07-12-2022 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Mycoplasmatota*
	Class	*Mollicutes*
	Order	*Acholeplasmatales*
	Family	*Acholeplasmataceae*
	Genus	*Acholeplasma*
	Species	**Acholeplasma equirhinis**
	Full Scientific Name (PNU)	Acholeplasma equirhinis Volokhov et al. 2020

Information on morphological and physiological properties Morphology

[Ref.: #68267]	Colony size	1 mm
[Ref.: #68267]	Colony shape	fried-egg-shaped
[Ref.: #68267]	Incubation period	5-7 days
[Ref.: #68267]	Cultivation medium used	PPLO agar

Information on culture and growth conditions Culture and growth conditions

[Ref.: #66035]

Culture medium

SP4-Z MEDIUM (DSMZ Medium 1076b)

[Ref.: #66035]

Culture medium growth

[Ref.: #66035]

Culture medium link

Medium recipe provided by DSMZ

[Ref.: #66035]

Culture medium composition

expand / minimize

		Temperature
[Ref.: #66035]	growth	35 °C
[Ref.: #68267]	growth	22-41 °C
[Ref.: #68267]	optimum	35-37 °C

[Ref.: #66035]	Temperature range	mesophilic
[Ref.: #68267]	Temperature range	mesophilic

Information on physiology and metabolism Physiology and metabolism

[Ref.: #68267]

Oxygen tolerance

facultative anaerobe

[Ref.: #68267]	Observation	Bacterial cells lack a cell wall
[Ref.: #68267]	Observation	Serum or sterol is required for sustained growth

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #68267]	arginine ChEBI	-	hydrolysis
[Ref.: #68267]	glucose ChEBI	+	builds acid from
[Ref.: #68267]	urea ChEBI	-	hydrolysis

	Antibiotica	Metabolite	Antibiotic resistance	Concentration
[Ref.: #68267]		penicillin g ChEBI	yes	1.5-2.0	g/L

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #66035]	Sample type/isolated from	nasopharyngeal swab of a horse
[Ref.: #66035]	Country	United Kingdom
[Ref.: #66035]	Country ISO 3 Code	GBR
[Ref.: #66035]	Continent	Europe
[Ref.: #66035]	Geographic location	55.3618°/-3.4433°

[Ref.: #68267]	Sample type/isolated from	nasopharynx of a thoroughbred horse (Equus caballus)
[Ref.: #68267]	Country	United Kingdom
[Ref.: #68267]	Country ISO 3 Code	GBR
[Ref.: #68267]	Continent	Europe
[Ref.: #68267]	Enrichment culture	PPLO broth
[Ref.: #68267]	Enrichment culture composition	containing glucose and penicillin (1.5-2 mg/mL)
[Ref.: #68267]	Enrichment culture duration	1-2 weeks
[Ref.: #68267]	Enrichment culture temperature	36 ̊C
* marker position based on {}

Isolation sources categories

#Host	#Mammals	#Equidae (Horse)
#Host Body-Site	#Organ	#Nose
#Infection	#Patient	#Swab

What are isolation sources categories?

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #66035]	Acholeplasma equirhinis strain N93 16S ribosomal RNA gene and 16S-23S ribosomal RNA intergenic spacer, partial sequence	EU925159	1546	ENA	555393 tax ID
[Ref.: #66035]	Acholeplasma equirhinis strain N93 23S ribosomal RNA gene, partial sequence; 23S-5S ribosomal RNA intergenic spacer, complete sequence; and 5S ribosomal RNA gene, partial sequence	GU905035	2890	ENA	555393 tax ID
[Ref.: #66035]	Acholeplasma equirhinis strain N93 RNA polymerase beta subunit gene, partial cds	FJ226562	2098	ENA	555393 tax ID

Genome sequence information:

	Sequence accession description	Seq. accession number	Assembly level (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #66792]	Acholeplasma equirhinis N93	GCA_017052655	contig	GenBank	555393 tax ID	*

[Ref.: #68267]

GC-content

36.83 mol%

high performance liquid chromatography (HPLC)

Availability in culture collections External links

[Ref.: #66035]

Culture collection no.

DSM 106692, NCTC 14351, ATCC TSD-139

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Acholeplasma equirhinis sp. nov. isolated from respiratory tract of horse (Equus caballus) and Mycoplasma procyoni sp. nov. isolated from oral cavity of raccoon (Procyon lotor).	Volokhov DV, Gao Y, Davidson MK, Chizhikov VE	Arch Microbiol	10.1007/s00203-019-01786-x	2019	*

References

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#66035

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 106692

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#68267

Dmitriy V. Volokhov, Yamei Gao, Maureen K. Davidson & Vladimir E. Chizhikov: Acholeplasma equirhinis sp. nov. isolated from respiratory tract of horse (Equus caballus) and Mycoplasma procyoni sp. nov. isolated from oral cavity of raccoon (Procyon lotor). Arch Microbiol 202: 411 - 420 2020 ( DOI 10.1007/s00203-019-01786-x )

#69480

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

* These data were automatically processed and therefore are not curated

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