Clostridioides difficile (DSM 101085, 3022, CD3022)
BacDive ID
158911
Type strain
Strain Designation
3022, CD3022
NCBI tax ID(s)
1496
History
DSMZ U. Groß, Institut für Medizinische Mikrobiologie. Universitätsmedizin Göttingen.; 3022; CD3022
Links
Clostridioides difficile 3022 is a human pathogen that was isolated from Faeces from a symptomatic patient.
human pathogen genome sequence BacteriaName and taxonomic classification
| @ref 20215 |
|
Last LPSN update
2026-05-29 08:44:04 |
| Domain Bacteria |
| Phylum Bacillota |
| Class Clostridia |
| Order Peptostreptococcales |
| Family Peptostreptococcaceae |
| Genus Clostridioides |
| Species Clostridioides difficile |
| Full scientific name Clostridioides difficile (Hall and O'Toole 1935) Lawson et al. 2016 |
| Synonyms (3) |
Culture and growth conditions
Culture medium
| @ref | Name | Growth | Medium link | Composition | |
|---|---|---|---|---|---|
| 65733 | WILKINS-CHALGREN ANAEROBE BROTH (DSMZ Medium 339) | Medium recipe at MediaDive  | Name: WILKINS-CHALGREN ANAEROBE BROTH (DSMZ Medium 339) Composition: dehydrated Wilkins-Chalgren medium 33.0 g/l L-Cysteine HCl 0.3 g/l Sodium resazurin 0.0005 g/l Distilled water | ||
| 65733 | COLUMBIA BLOOD MEDIUM (DSMZ Medium 693) | Medium recipe at MediaDive | Name: COLUMBIA BLOOD MEDIUM (DSMZ Medium 693) Composition: Defibrinated sheep blood 50.0 g/l Columbia agar base | ||
| 65733 | FASTIDIOUS ANAEROBE AGAR (DSMZ Medium 1203) | Medium recipe at MediaDive | Name: FASTIDIOUS ANAEROBE AGAR (DSMZ Medium 1203) Composition: Horse blood 100.0 g/l Fastidious Anaerobe Agar 45.7 g/l Distilled water |
Culture temp
| @ref | Growth | Type | Temperature (°C) | |
|---|---|---|---|---|
| 65733 | positive | growth | 37 |
Physiology and metabolism
Oxygen tolerance
| @ref | Oxygen tolerance | Confidence | |
|---|---|---|---|
| 125439 | facultative anaerobe | 98.198 |
Metabolite utilization
| @ref | Chebi-ID | Metabolite | Utilization activity | Kind of utilization tested | |
|---|---|---|---|---|---|
| 68380 | 29016 ChEBI | arginine | - | hydrolysis | from API rID32A |
| 68367 | 17057 ChEBI | cellobiose | - | builds acid from | from API 20A |
| 68380 | 16024 ChEBI | D-mannose | - | fermentation | from API rID32A |
| 68367 | 65327 ChEBI | D-xylose | - | builds acid from | from API 20A |
| 68367 | 17754 ChEBI | glycerol | - | builds acid from | from API 20A |
| 68367 | 30849 ChEBI | L-arabinose | - | builds acid from | from API 20A |
| 68380 | 29985 ChEBI | L-glutamate | - | degradation | from API rID32A |
| 68367 | 62345 ChEBI | L-rhamnose | - | builds acid from | from API 20A |
| 68367 | 17716 ChEBI | lactose | - | builds acid from | from API 20A |
| 68367 | 17306 ChEBI | maltose | - | builds acid from | from API 20A |
| 68367 | 6731 ChEBI | melezitose | - | builds acid from | from API 20A |
| 68380 | 17632 ChEBI | nitrate | - | reduction | from API rID32A |
| 68380 | 16634 ChEBI | raffinose | - | fermentation | from API rID32A |
| 68367 | 16634 ChEBI | raffinose | - | builds acid from | from API 20A |
| 68367 | 17814 ChEBI | salicin | - | builds acid from | from API 20A |
| 68367 | 30911 ChEBI | sorbitol | - | builds acid from | from API 20A |
| 68367 | 17992 ChEBI | sucrose | - | builds acid from | from API 20A |
| 68367 | 27082 ChEBI | trehalose | - | builds acid from | from API 20A |
| 68380 | 27897 ChEBI | tryptophan | - | energy source | from API rID32A |
| 68367 | 27897 ChEBI | tryptophan | - | energy source | from API 20A |
| 68380 | 16199 ChEBI | urea | - | hydrolysis | from API rID32A |
| 68367 | 16199 ChEBI | urea | - | hydrolysis | from API 20A |
Metabolite production
Metabolite tests
Enzymes
| @ref | Value | Activity | Ec | |
|---|---|---|---|---|
| 68380 | alanine arylamidase | - | 3.4.11.2 | from API rID32A |
| 68380 | alkaline phosphatase | - | 3.1.3.1 | from API rID32A |
| 68380 | alpha-arabinosidase | - | 3.2.1.55 | from API rID32A |
| 68380 | alpha-fucosidase | - | 3.2.1.51 | from API rID32A |
| 68380 | alpha-galactosidase | - | 3.2.1.22 | from API rID32A |
| 68380 | alpha-glucosidase | - | 3.2.1.20 | from API rID32A |
| 68380 | arginine dihydrolase | - | 3.5.3.6 | from API rID32A |
| 68380 | beta-galactosidase | - | 3.2.1.23 | from API rID32A |
| 68380 | beta-Galactosidase 6-phosphate | - | from API rID32A | |
| 68380 | beta-glucosidase | - | 3.2.1.21 | from API rID32A |
| 68380 | beta-glucuronidase | - | 3.2.1.31 | from API rID32A |
| 68380 | glutamate decarboxylase | - | 4.1.1.15 | from API rID32A |
| 68380 | glutamyl-glutamate arylamidase | - | from API rID32A | |
| 68380 | glycin arylamidase | - | from API rID32A | |
| 68380 | histidine arylamidase | - | from API rID32A | |
| 68380 | L-arginine arylamidase | - | from API rID32A | |
| 68380 | leucine arylamidase | - | 3.4.11.1 | from API rID32A |
| 68380 | leucyl glycin arylamidase | - | 3.4.11.1 | from API rID32A |
| 68380 | N-acetyl-beta-glucosaminidase | - | 3.2.1.52 | from API rID32A |
| 68380 | phenylalanine arylamidase | - | from API rID32A | |
| 68380 | proline-arylamidase | + | 3.4.11.5 | from API rID32A |
| 68380 | pyrrolidonyl arylamidase | - | 3.4.19.3 | from API rID32A |
| 68380 | serine arylamidase | - | from API rID32A | |
| 68380 | tryptophan deaminase | - | 4.1.99.1 | from API rID32A |
| 68380 | tyrosine arylamidase | - | from API rID32A | |
| 68367 | urease | - | 3.5.1.5 | from API 20A |
| 68380 | urease | - | 3.5.1.5 | from API rID32A |
API 20A
| @ref | IND | URE | GLU | MAN | LAC | SAC | MAL | SAL | XYL | ARA | GEL | ESC | GLY | CEL | MNE | MLZ | RAF | SOR | RHA | TRE | CAT | Spores presentSPOR | GramGRAM | Morphology coccus="+" rod="-"COCC | |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| 65733 | - | - | - | - | - | - | - | - | - | - | - | +/- | - | - | - | - | - | - | - | - | not determinedn.d. | not determinedn.d. | not determinedn.d. | not determinedn.d. | |
| 65733 | - | - | + | + | - | - | - | - | - | - | +/- | + | - | - | +/- | - | - | - | - | - | not determinedn.d. | not determinedn.d. | not determinedn.d. | not determinedn.d. |
API rID32A
| @ref | URE | ADH (Arg) | alpha GAL | beta GAL | beta-Galactosidase 6-phosphatebeta GP | alpha GLU | beta GLU | alpha ARA | beta GUR | beta-N-Acetyl-beta-glucosaminidasebeta NAG | MNE | RAF | GDC | alpha FUC | Reduction of nitrateNIT | IND | PAL | L-arginine arylamidaseArgA | ProA | LGA | Phenylalanine arylamidasePheA | Leucine arylamidaseLeuA | PyrA | Tyrosine arylamidaseTyrA | Alanine arylamidaseAlaA | Glycin arylamidaseGlyA | Histidine arylamidaseHisA | Glutamyl-glutamate arylamidaseGGA | Serine arylamidaseSerA | |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| 65733 | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | + | - | - | - | - | - | - | - | - | - | - | |
| 65733 | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | + | - | - | - | - | - | - | - | - | - | - |
Sequence information
Genome sequences
| @ref | Description | Assembly level | INSDC accession | BV-BRC accession | NCBI tax ID | Score | |
|---|---|---|---|---|---|---|---|
| 66792 | ASM1573253v1 assembly for Clostridioides difficile DSM 101085 | complete | GCA_015732535   | 1496.5622  | 1496 | 97.58 | |
Genome browser: GCA_015732535
Literature
Literature 
| Topic | Title | Authors | Journal | DOI | Year | |
|---|---|---|---|---|---|---|
| Genomic analysis of a novel ST11(PR34365) Clostridioides difficile strain isolated from the human fecal of a CDI patient in Guizhou, China. | Yang Y, Shu L, Ling P, Yang J, Shao R, Cheng Y, Luo S, Wei X, Guan Z, Chen Z, Liao J, Qi X, Cui G, Hong W. | Open Life Sci | 10.1515/biol-2025-1067 | 2025 | | |
| Draft Genome Sequences and Genome Characterization of Three Toxigenic and Two Nontoxigenic Clostridioides difficile Clinical Isolates from Florida, USA. | Wickramage I, Heuler J, Peng Z, Alrabaa S, Sun X. | Microbiol Resour Announc | 10.1128/mra.00151-23 | 2023 | | |
| Phenotype | Characterization of Clostridioides difficile DSM 101085 with A-B-CDT+ Phenotype from a Late Recurrent Colonization. | Riedel T, Neumann-Schaal M, Wittmann J, Schober I, Hofmann JD, Lu CW, Dannheim A, Zimmermann O, Lochner M, Gross U, Overmann J. | Genome Biol Evol | 10.1093/gbe/evaa072 | 2020 | |
| Assessment of the Transmission Dynamics of Clostridioides difficile in a Farm Environment Reveals the Presence of a New Toxigenic Strain Connected to Swine Production. | Alves F, Nunes A, Castro R, Sequeira A, Moreira O, Matias R, Rodrigues JC, Silveira L, Gomes JP, Oleastro M. | Front Microbiol | 10.3389/fmicb.2022.858310 | 2022 | |
How to cite
If you want to cite this particular strain cite the following doi:
https://doi.org/10.13145/bacdive158911.20260601.11
When using BacDive for research please cite the following paper
BacDive in 2025: the core database for prokaryotic strain dataIsabel Schober, Julia Koblitz, Joaquim Sardà Carbasse, Christian Ebeling, Marvin Leon Schmidt, Adam Podstawka, Rohit Gupta, Vinodh Ilangovan, Javad Chamanara, Jörg Overmann, Lorenz Christian Reimer
Nucleic Acids Research; database issue 2025.
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