Name: Conexivisphaera calida NAS-02
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 158879

Type strain:

Species: Conexivisphaera calida

Strain Designation: NAS-02

Culture col. no.: DSM 105898, JCM 31663

Strain history: T. Itoh: RIKEN BRC-JCM, Japan; NAS-02.

NCBI tax ID(s): 1874277 (species)

SI-ID 404740

DSM 105898

Section

Conexivisphaera calida NAS-02 is an anaerobe, thermophilic, Gram-negative archaeon that was isolated from Hot spring.

Gram-negative
anaerobe
thermophilic
16S sequence
Archaea
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Archaea"
	Phylum	*Nitrososphaerota*
	Class	*Conexivisphaeria*
	Order	*Conexivisphaerales*
	Family	*Conexivisphaeraceae*
	Genus	*Conexivisphaera*
	Species	**Conexivisphaera calida**
	Full Scientific Name (LPSN)	Conexivisphaera calida Kato et al. 2021

Synonym

"Conexivisphaera calidus"

Information on morphological and physiological properties Morphology

[Ref.: #69480]	Gram stain	negative Confidence in %99.837

Information on culture and growth conditions Culture and growth conditions

[Ref.: #65701]

Culture medium

CONEXIVISPHAERA MEDIUM (DSMZ Medium 1714)

[Ref.: #65701]

Culture medium growth

[Ref.: #65701]

Culture medium link

Medium recipe at MediaDive

[Ref.: #65701]

Culture medium composition

expand / minimize

	Temperatures	Kind of temperature		Temperature
[Ref.: #65701]			growth	70 °C
[Ref.: #67770]			growth	65 °C

[Ref.: #65701]	Temperature range	thermophilic
[Ref.: #67770]	Temperature range	thermophilic
[Ref.: #69480]	Temperature range	thermophilic Confidence in %99.73

Information on physiology and metabolism Physiology and metabolism

[Ref.: #69480]

Oxygen tolerance

anaerobe Confidence in %98.544

[Ref.: #69480]	Ability of spore formation	no Confidence in %99.971
[Ref.: #69481]	Ability of spore formation	yes Confidence in %100

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #65701]	Sample type/isolated from	Hot spring
[Ref.: #65701]	Geographic location (country and/or sea, region)	Oku-Shiobara, Tochigi
[Ref.: #65701]	Country	Japan
[Ref.: #65701]	Country ISO 3 Code	JPN
[Ref.: #65701]	Continent	Asia

[Ref.: #67770]	Sample type/isolated from	Hot spring water from Oku-Shiobara
[Ref.: #67770]	Geographic location (country and/or sea, region)	Tochigi
[Ref.: #67770]	Country	Japan
[Ref.: #67770]	Country ISO 3 Code	JPN
[Ref.: #67770]	Continent	Asia
* marker position based on {}

Isolation sources categories

#Environmental	#Aquatic	#Thermal spring
#Condition	#Thermophilic (>45°C)	-

What are isolation sources categories?

[Ref.: #69479]

Global distribution of 16S sequence LC552068 (>99% sequence identity) for Conexivisphaera calida subclade from Microbeatlas

Aquatic Samples	24
Soil Samples
Animal Samples
Plant Samples
Total Samples	24

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #67770]	Conexivisphaera calidus NAS-02 gene for 16S rRNA, partial sequence	LC552068	1508	ENA	1874277 tax ID

Genome sequence information:

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #66792]	Conexivisphaera calida NAS-02	GCA_013340765	complete	GenBank	1874277 tax ID	*

[Ref.: #65701]	GC-content	62.1 mol%	sequence analysis
[Ref.: #67770]	GC-content	62.1 mol%	genome sequence analysis

Data predicted using genome information as a basis Genome-based predictions

	Trait	Prediction	Confidence in %	In training data
	Predictions from GenomeNet Sporulation v. 1 based on: Conexivisphaera calida NAS-02 NCBI: GCA_013340765.1
[Ref.: #69481]	spore-forming	yes	100	no

	Trait	Prediction	Confidence in %	In training data
	Predictions from Diaspora project based on: Conexivisphaera calida NAS-02 NCBI: GCA_013340765.1
[Ref.: #69480]	motile	no	85.045	no
[Ref.: #69480]	flagellated	no	97.571	no
[Ref.: #69480]	gram-positive	no	96.833	no
[Ref.: #69480]	anaerobic	yes	98.264	no
[Ref.: #69480]	aerobic	no	93.7	no
[Ref.: #69480]	halophile	no	80.777	no
[Ref.: #69480]	spore-forming	no	97.791	no
[Ref.: #69480]	glucose-util	yes	78.718	no
[Ref.: #69480]	thermophile	yes	99.999	yes
[Ref.: #69480]	glucose-ferment	no	62.771	no

Availability in culture collections External links

[Ref.: #65701]

Culture collection no.

DSM 105898, JCM 31663

[Ref.: #111398]

SI-ID 404740

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Conexivisphaera calida gen. nov., sp. nov., a thermophilic sulfur- and iron-reducing archaeon, and proposal of Conexivisphaeraceae fam. nov., Conexivisphaerales ord. nov., and Conexivisphaeria class. nov. in the phylum Thaumarchaeota.	Kato S, Ohnishi M, Nagamori M, Yuki M, Takashina T, Ohkuma M, Itoh T	Int J Syst Evol Microbiol	10.1099/ijsem.0.004595	2020	*
Phylogeny	Isolation and characterization of a thermophilic sulfur- and iron-reducing thaumarchaeote from a terrestrial acidic hot spring.	Kato S, Itoh T, Yuki M, Nagamori M, Ohnishi M, Uematsu K, Suzuki K, Takashina T, Ohkuma M	ISME J	10.1038/s41396-019-0447-3	2019	*

References

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#65701

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 105898

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#67770 Japan Collection of Microorganism (JCM) ; Curators of the JCM;

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

#69480

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#69481

Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch: Predictions based on the model GenomeNet Sporulation v. 1 . ( DOI 10.21203/rs.3.rs-2527258/v1 )

#111398

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID404740.1 )

* These data were automatically processed and therefore are not curated

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