Name: Neptunicoccus sediminis CCTCC AB 2015430
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 158806

Type strain:

Species: Neptunicoccus sediminis

Culture col. no.: CCTCC AB 2015430, KCTC 42985, NBRC 111872, MCCC 1K03518, CY 02

Strain history: <- Yan-Jiao Zhang, Qingdao Agricultural Univ.

NCBI tax ID(s): 1892596 (species)

Section

Neptunicoccus sediminis CCTCC AB 2015430 is an aerobe, halophilic, Gram-negative bacterium that forms circular colonies and was isolated from surface marine sediment.

coccus-shaped
colony-forming
Gram-negative
aerobe
halophilic
16S sequence
Bacteria

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Pseudomonadota*
	Class	*Alphaproteobacteria*
	Order	*Rhodobacterales*
	Family	*Roseobacteraceae*
	Genus	*Neptunicoccus*
	Species	**Neptunicoccus sediminis**
	Full Scientific Name (LPSN)	Neptunicoccus sediminis Zhang et al. 2018

Information on morphological and physiological properties Morphology

[Ref.: #65568]	Gram stain	negative

[Ref.: #65568]	Cell length	0.8-1.8 µm

[Ref.: #65568]	Cell width	0.5-1.6 µm

[Ref.: #65568]	Cell shape	coccus-shaped

[Ref.: #65568]	Colony size	0.8-1.5 mm
[Ref.: #65568]	Colony shape	circular
[Ref.: #65568]	Incubation period	3 days
[Ref.: #65568]	Cultivation medium used	TYS

Information on culture and growth conditions Culture and growth conditions

[Ref.: #65568]

Culture medium

TYS

[Ref.: #65568]

Culture medium growth

		Temperature
[Ref.: #65568]	growth	5-42 °C
[Ref.: #65568]	optimum	35 °C
[Ref.: #67771]	growth	30 °C

[Ref.: #65568]	Temperature range	mesophilic
[Ref.: #67771]	Temperature range	mesophilic

	pH	Kind of pH		pH
[Ref.: #65568]			optimum	8.0
[Ref.: #65568]			growth	6-10

Information on physiology and metabolism Physiology and metabolism

[Ref.: #65568]	Oxygen tolerance	aerobe
[Ref.: #67771]	Oxygen tolerance	aerobe

[Ref.: #65568]

Halophily / tolerance level

halophilic

	Salt	Tested relation		Salt conc.
[Ref.: #65568]	NaCl		growth	0.5-9.0	%(w/v)
[Ref.: #65568]	NaCl		optimum	1.5-3.0	%(w/v)
[Ref.: #65568]	NaCl	no	growth	0	%(w/v)

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #65568]	amygdalin ChEBI	-	builds acid from
[Ref.: #65568]	arabinose ChEBI	-	assimilation
[Ref.: #65568]	arbutin ChEBI	-	builds acid from
[Ref.: #65568]	casein	-	hydrolysis
[Ref.: #65568]	cellobiose ChEBI	-	builds acid from
[Ref.: #65568]	citrate ChEBI	-	assimilation
[Ref.: #65568]	D-arabinose ChEBI	-	builds acid from
[Ref.: #65568]	D-arabitol ChEBI	-	builds acid from
[Ref.: #65568]	D-fructose ChEBI	-	builds acid from
[Ref.: #65568]	D-fucose ChEBI	-	builds acid from
[Ref.: #65568]	D-galactose ChEBI	-	builds acid from
[Ref.: #65568]	D-glucose ChEBI	-	builds acid from
[Ref.: #65568]	D-lyxose ChEBI	-	builds acid from
[Ref.: #65568]	D-mannitol ChEBI	-	builds acid from
[Ref.: #65568]	D-mannose ChEBI	-	builds acid from
[Ref.: #65568]	D-ribose ChEBI	-	builds acid from
[Ref.: #65568]	D-sorbitol ChEBI	-	builds acid from
[Ref.: #65568]	D-tagatose ChEBI	-	builds acid from
[Ref.: #65568]	D-xylose ChEBI	-	builds acid from
[Ref.: #65568]	decanoate ChEBI	-	assimilation
[Ref.: #65568]	dipic acid	-	assimilation
[Ref.: #65568]	erythritol ChEBI	-	builds acid from
[Ref.: #65568]	esculin ferric citrate	-	builds acid from
[Ref.: #65568]	galactitol ChEBI	-	builds acid from
[Ref.: #65568]	gentiobiose ChEBI	-	builds acid from
[Ref.: #65568]	gluconate ChEBI	-	assimilation
[Ref.: #65568]	glucose ChEBI	-	assimilation
[Ref.: #65568]	glucose ChEBI	-	builds acid from
[Ref.: #65568]	glycerol ChEBI	-	builds acid from
[Ref.: #65568]	glycogen ChEBI	-	builds acid from
[Ref.: #65568]	hypoxanthine ChEBI	-	hydrolysis
[Ref.: #65568]	inulin ChEBI	-	builds acid from
[Ref.: #65568]	L-arabinose ChEBI	-	builds acid from
[Ref.: #65568]	L-arabitol ChEBI	-	builds acid from
[Ref.: #65568]	L-fucose ChEBI	-	builds acid from
[Ref.: #65568]	L-rhamnose ChEBI	-	builds acid from
[Ref.: #65568]	L-sorbose ChEBI	-	builds acid from
[Ref.: #65568]	L-xylose ChEBI	-	builds acid from
[Ref.: #65568]	lactose ChEBI	-	builds acid from
[Ref.: #65568]	lecithin ChEBI	-	hydrolysis
[Ref.: #65568]	malate ChEBI	-	assimilation
[Ref.: #65568]	maltose ChEBI	-	assimilation
[Ref.: #65568]	maltose ChEBI	-	builds acid from
[Ref.: #65568]	mannitol ChEBI	-	assimilation
[Ref.: #65568]	mannose ChEBI	-	assimilation
[Ref.: #65568]	melezitose ChEBI	-	builds acid from
[Ref.: #65568]	melibiose ChEBI	-	builds acid from
[Ref.: #65568]	methyl alpha-D-glucopyranoside ChEBI	-	builds acid from
[Ref.: #65568]	methyl alpha-D-mannoside ChEBI	-	builds acid from
[Ref.: #65568]	methyl beta-D-xylopyranoside ChEBI	-	builds acid from
[Ref.: #65568]	myo-inositol ChEBI	-	builds acid from
[Ref.: #65568]	N-acetylglucosamine ChEBI	-	assimilation
[Ref.: #65568]	N-acetylglucosamine ChEBI	-	builds acid from
[Ref.: #65568]	nitrate ChEBI	-	reduction
[Ref.: #65568]	phenylacetate ChEBI	-	assimilation
[Ref.: #65568]	potassium 2-dehydro-D-gluconate	-	builds acid from
[Ref.: #65568]	potassium 5-dehydro-D-gluconate	-	builds acid from
[Ref.: #65568]	potassium gluconate ChEBI	-	builds acid from
[Ref.: #65568]	raffinose ChEBI	-	builds acid from
[Ref.: #65568]	ribitol ChEBI	-	builds acid from
[Ref.: #65568]	salicin ChEBI	-	builds acid from
[Ref.: #65568]	starch ChEBI	-	builds acid from
[Ref.: #65568]	starch ChEBI	-	hydrolysis
[Ref.: #65568]	sucrose ChEBI	-	builds acid from
[Ref.: #65568]	trehalose ChEBI	-	builds acid from
[Ref.: #65568]	turanose ChEBI	-	builds acid from
[Ref.: #65568]	tween 40 ChEBI	-	hydrolysis
[Ref.: #65568]	tween 60 ChEBI	-	hydrolysis
[Ref.: #65568]	tween 80 ChEBI	-	hydrolysis
[Ref.: #65568]	xanthine ChEBI	-	hydrolysis
[Ref.: #65568]	xylitol ChEBI	-	builds acid from
	Only first 10 entries are displayed. Click here to see all.

	Metabolite	Antibiotic sensitivity	Concentration
[Ref.: #65568]	amoxicillin ChEBI	yes	10	µg (disc)
[Ref.: #65568]	ampicillin ChEBI	yes	10	µg (disc)
[Ref.: #65568]	bacitracin ChEBI	yes	10	Unit (disc)
[Ref.: #65568]	carbenicillin ChEBI	yes	100	µg (disc)
[Ref.: #65568]	cefotaxime ChEBI	yes	30	µg (disc)
[Ref.: #65568]	cefoxitin ChEBI	yes	30	µg (disc)
[Ref.: #65568]	chloramphenicol ChEBI	yes	30	µg (disc)
[Ref.: #65568]	neomycin ChEBI	yes	30	µg (disc)
[Ref.: #65568]	nitrofurantoin ChEBI	yes	300	µg (disc)
[Ref.: #65568]	penicillin g ChEBI	yes	10	Unit (disc)
[Ref.: #65568]	polymyxin b ChEBI	yes	300	Unit (disc)
[Ref.: #65568]	rifampicin ChEBI	yes	5	µg (disc)
	Only first 10 entries are displayed. Click here to see all.

	Metabolite production	Metabolite	Production
[Ref.: #65568]		indole ChEBI	no

	Enzyme	Enzyme activity	EC number
[Ref.: #65568]	acid phosphatase	+	3.1.3.2
[Ref.: #65568]	alkaline phosphatase	+	3.1.3.1
[Ref.: #65568]	alpha-chymotrypsin	-	3.4.21.1
[Ref.: #65568]	alpha-fucosidase	-	3.2.1.51
[Ref.: #65568]	alpha-galactosidase	-	3.2.1.22
[Ref.: #65568]	alpha-glucosidase	-	3.2.1.20
[Ref.: #65568]	alpha-mannosidase	-	3.2.1.24
[Ref.: #65568]	arginine dihydrolase	-	3.5.3.6
[Ref.: #65568]	beta-galactosidase	-	3.2.1.23
[Ref.: #65568]	beta-glucosidase	-	3.2.1.21
[Ref.: #65568]	beta-glucuronidase	-	3.2.1.31
[Ref.: #65568]	catalase	+	1.11.1.6
[Ref.: #65568]	cystine arylamidase	+	3.4.11.3
[Ref.: #65568]	cytochrome oxidase	+	1.9.3.1
[Ref.: #65568]	esterase (C 4)	+
[Ref.: #65568]	esterase Lipase (C 8)	+
[Ref.: #65568]	gelatinase	-
[Ref.: #65568]	leucine arylamidase	+	3.4.11.1
[Ref.: #65568]	lipase (C 14)	-
[Ref.: #65568]	N-acetyl-beta-glucosaminidase	-	3.2.1.52
[Ref.: #65568]	naphthol-AS-BI-phosphohydrolase	+
[Ref.: #65568]	trypsin	-	3.4.21.4
[Ref.: #65568]	urease	-	3.5.1.5
[Ref.: #65568]	valine arylamidase	+
	Only first 10 entries are displayed. Click here to see all.

Fatty acid profile

Metadata FA analysis

Reference

[Ref.: #65568]

Fatty acid	Percentage
C18:1ω7c	57.6
11-methyl C18:1ω7c	22.8
C16:0	10.6
unknown 11.799	5

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #65568]	Sample type/isolated from	surface marine sediment
[Ref.: #65568]	Sampling date	2014-11-19
[Ref.: #65568]	Geographic location (country and/or sea, region)	Site B26, Yellow Sea
[Ref.: #65568]	Geographic location	37.6900°/121.9800°
[Ref.: #65568]	Enrichment culture	TYM
[Ref.: #65568]	Enrichment culture composition	containing 0.5 % tryptone, 0.1 % yeast extract, 0.5 % skim milk, 1.5 % agar and artificial seawater, pH 7.5
[Ref.: #65568]	Enrichment culture duration	15 days
[Ref.: #65568]	Enrichment culture temperature	15 ̊C
[Ref.: #65568]	Isolation/enrichment procedure	approximately 1 g (wet weight) sediment was mixed with 99 ml sterilized artificial seawater (ASW) and 16 g glass beads (diameter 6-8 mm). The mixture was shaken at 15 °C and 150 rpm for 0.5 h and serially diluted (to 10-6) in artifical seawater. 70 µl aliquots were spread onto TYM

[Ref.: #67771]	Sample type/isolated from	From the sediments of the Yellow Sea.
[Ref.: #67771]	Country	China
[Ref.: #67771]	Country ISO 3 Code	CHN
[Ref.: #67771]	Continent	Asia
* marker position based on {}

Isolation sources categories

#Environmental	#Aquatic	#Marine
#Environmental	#Aquatic	#Sediment

What are isolation sources categories?

[Ref.: #69479]

Global distribution of 16S sequence KY777997 (>99% sequence identity) for Neptunicoccus sediminis subclade from Microbeatlas

Aquatic Samples	743
Soil Samples	48
Animal Samples	105
Plant Samples	9
Total Samples	905

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #65568]	16S rRNA gene sequence	KY777997		GenBank

[Ref.: #65568]

GC-content

57.5 mol%

genome sequence analysis

Availability in culture collections External links

[Ref.: #65568]

Culture collection no.

CCTCC AB 2015430, KCTC 42985, NBRC 111872, MCCC 1K03518, CY 02

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Neptunicoccus sediminis gen. nov., sp. nov., a member of the family Rhodobacteraceae isolated from the Yellow Sea.	Zhang YJ, Liu XF, Kuang BZ, Zhang XY, Zhou MY, Chen S	Int J Syst Evol Microbiol	10.1099/ijsem.0.002728	2018	*

References

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#65568

Yan-Jiao Zhang, Xu-Feng Liu, Bao-Zhi Kuang, Xi-Ying Zhang, Ming-Yang Zhou and Shiyong Chen: Neptunicoccus sediminis gen. nov., sp. nov., a member of the family Rhodobacteraceae isolated from the Yellow Sea. IJSEM 68: 1702 - 1706 2018 ( DOI 10.1099/ijsem.0.002728 , PubMed 29580372 )

#67771 Korean Collection for Type Cultures (KCTC) ; Curators of the KCTC;

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

* These data were automatically processed and therefore are not curated

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