Name: Halomonas malpeensis YU-PRIM-29
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 158781

Type strain:

Species: Halomonas malpeensis

Strain Designation: YU-PRIM-29

Culture col. no.: LMG 28855, CCM 8737, CIP 111286

Strain history: CIP <- 2016, P. Kämpfer, Giessen Univ., Giessen, Germany: strain YU-PRIM-29

NCBI tax ID(s): 1172368 (species)

Section

Halomonas malpeensis YU-PRIM-29 is an obligate aerobe, mesophilic, Gram-negative bacterium that forms circular colonies and was isolated from rhizosphere of sand dune coastal plant.

colony-forming
Gram-negative
motile
rod-shaped
mesophilic
obligate aerobe
16S sequence
Bacteria

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Pseudomonadota*
	Class	*Gammaproteobacteria*
	Order	*Oceanospirillales*
	Family	*Halomonadaceae*
	Genus	*Halomonas*
	Species	**Halomonas malpeensis**
	Full Scientific Name (LPSN)	Halomonas malpeensis Kämpfer et al. 2018

Synonym

"Vreelandella malpeensis"

Information on morphological and physiological properties Morphology

[Ref.: #65535]	Gram stain	negative
[Ref.: #120764]	Gram stain	negative

[Ref.: #65535]	Cell length	2.0-3.0 µm

[Ref.: #65535]	Cell width	0.8-1.0 µm

[Ref.: #65535]	Cell shape	rod-shaped
[Ref.: #120764]	Cell shape	rod-shaped

[Ref.: #65535]	Motility	yes
[Ref.: #120764]	Motility	yes

[Ref.: #65535]	Flagellum arrangement	polar

[Ref.: #65535]	Colony color	yellow
[Ref.: #65535]	Colony shape	circular
[Ref.: #65535]	Cultivation medium used	MA

Information on culture and growth conditions Culture and growth conditions

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Culture medium

TSB

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Culture medium growth

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Culture medium

Marine agar (MA)

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Culture medium growth

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Culture medium

CIP Medium 72

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Culture medium growth

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Culture medium link

Medium recipe provided by DSMZ

	Kind of temperature		Temperature
[Ref.: #65535]		optimum	25-30 °C
[Ref.: #65535]		growth	10-36 °C
[Ref.: #65535]	no	growth	4 °C
[Ref.: #65535]	no	growth	45 °C

[Ref.: #65535]	Temperature range	mesophilic
[Ref.: #65535]	Temperature range	psychrophilic
[Ref.: #65535]	Temperature range	thermophilic

	pH	Kind of pH		pH
[Ref.: #65535]			optimum	7-9
[Ref.: #65535]			growth	6.0-12.0

Information on physiology and metabolism Physiology and metabolism

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Oxygen tolerance

obligate aerobe

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Ability of spore formation

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Halophily / tolerance level

halophilic

	Halophily	Salt	Tested relation		Salt conc.
[Ref.: #65535]		NaCl		optimum	0.5-5	%(w/v)
[Ref.: #65535]		NaCl		growth	0.5-20	%(w/v)

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #65535]	2-oxoglutarate ChEBI	-	carbon source
[Ref.: #65535]	4-hydroxybenzoate ChEBI	-	carbon source
[Ref.: #65535]	acetate ChEBI	-	carbon source
[Ref.: #65535]	adipate ChEBI	-	carbon source
[Ref.: #65535]	adipate ChEBI	+	carbon source
[Ref.: #65535]	alanine ChEBI	-	carbon source
[Ref.: #65535]	amygdalin ChEBI	-	carbon source
[Ref.: #65535]	arbutin ChEBI	-	carbon source
[Ref.: #65535]	azelaate ChEBI	-	carbon source
[Ref.: #65535]	cellobiose ChEBI	-	builds acid from
[Ref.: #65535]	cellobiose ChEBI	-	carbon source
[Ref.: #65535]	cis-aconitate ChEBI	-	carbon source
[Ref.: #65535]	citrate ChEBI	-	assimilation
[Ref.: #65535]	citrate ChEBI	-	carbon source
[Ref.: #65535]	citrate ChEBI	+	carbon source
[Ref.: #65535]	D-arabitol ChEBI	-	builds acid from
[Ref.: #65535]	D-fructose ChEBI	-	carbon source
[Ref.: #65535]	D-fucose ChEBI	-	builds acid from
[Ref.: #65535]	D-fucose ChEBI	-	carbon source
[Ref.: #65535]	D-galactose ChEBI	-	builds acid from
[Ref.: #65535]	D-galactose ChEBI	-	carbon source
[Ref.: #65535]	D-glucose ChEBI	-	builds acid from
[Ref.: #65535]	D-glucose ChEBI	-	carbon source
[Ref.: #65535]	D-glucose ChEBI	+	carbon source
[Ref.: #65535]	D-mannitol ChEBI	-	carbon source
[Ref.: #65535]	D-mannose ChEBI	-	carbon source
[Ref.: #65535]	D-sorbitol ChEBI	-	builds acid from
[Ref.: #65535]	D-sorbitol ChEBI	-	carbon source
[Ref.: #65535]	D-xylose ChEBI	-	builds acid from
[Ref.: #65535]	D-xylose ChEBI	-	carbon source
[Ref.: #65535]	erythritol ChEBI	-	builds acid from
[Ref.: #65535]	galactitol ChEBI	-	builds acid from
[Ref.: #65535]	galactitol ChEBI	-	carbon source
[Ref.: #65535]	gamma-aminobutyric acid ChEBI	-	carbon source
[Ref.: #65535]	gluconate ChEBI	-	carbon source
[Ref.: #65535]	gluconate ChEBI	+	carbon source
[Ref.: #65535]	glycerol ChEBI	-	builds acid from
[Ref.: #65535]	glycogen ChEBI	-	carbon source
[Ref.: #65535]	histidine ChEBI	-	carbon source
[Ref.: #65535]	isoleucine ChEBI	-	carbon source
[Ref.: #65535]	itaconate ChEBI	-	carbon source
[Ref.: #65535]	L-arabinose ChEBI	-	builds acid from
[Ref.: #65535]	L-arabinose ChEBI	+	carbon source
[Ref.: #65535]	L-cysteine ChEBI	-	carbon source
[Ref.: #65535]	L-lysine ChEBI	-	carbon source
[Ref.: #65535]	L-methionine ChEBI	-	carbon source
[Ref.: #65535]	L-rhamnose ChEBI	-	builds acid from
[Ref.: #65535]	L-rhamnose ChEBI	-	carbon source
[Ref.: #65535]	L-valine ChEBI	-	carbon source
[Ref.: #65535]	lactose ChEBI	-	builds acid from
[Ref.: #65535]	lactose ChEBI	-	carbon source
[Ref.: #65535]	malate ChEBI	-	carbon source
[Ref.: #65535]	malate ChEBI	+	carbon source
[Ref.: #65535]	malonate ChEBI	-	carbon source
[Ref.: #65535]	maltitol ChEBI	-	carbon source
[Ref.: #65535]	maltose ChEBI	-	builds acid from
[Ref.: #65535]	maltose ChEBI	-	carbon source
[Ref.: #65535]	mannitol ChEBI	-	builds acid from
[Ref.: #65535]	melezitose ChEBI	-	carbon source
[Ref.: #65535]	melibiose ChEBI	-	builds acid from
[Ref.: #65535]	melibiose ChEBI	-	carbon source
[Ref.: #65535]	mesaconate ChEBI	-	carbon source
[Ref.: #65535]	methyl alpha-D-glucopyranoside ChEBI	-	builds acid from
[Ref.: #65535]	myo-inositol ChEBI	-	builds acid from
[Ref.: #65535]	myo-inositol ChEBI	-	carbon source
[Ref.: #65535]	N-acetylglucosamine ChEBI	-	carbon source
[Ref.: #65535]	nitrate ChEBI	-	reduction
[Ref.: #65535]	propionate ChEBI	-	carbon source
[Ref.: #65535]	putrescine ChEBI	-	carbon source
[Ref.: #65535]	pyruvate ChEBI	-	carbon source
[Ref.: #65535]	raffinose ChEBI	-	builds acid from
[Ref.: #65535]	ribitol ChEBI	-	builds acid from
[Ref.: #65535]	ribitol ChEBI	-	carbon source
[Ref.: #65535]	ribose ChEBI	-	carbon source
[Ref.: #65535]	salicin ChEBI	-	builds acid from
[Ref.: #65535]	salicin ChEBI	-	carbon source
[Ref.: #65535]	serine ChEBI	-	carbon source
[Ref.: #65535]	starch ChEBI	-	carbon source
[Ref.: #65535]	sucrose ChEBI	-	builds acid from
[Ref.: #65535]	sucrose ChEBI	-	carbon source
[Ref.: #65535]	trans-aconitate ChEBI	-	carbon source
[Ref.: #65535]	trehalose ChEBI	-	builds acid from
[Ref.: #65535]	trehalose ChEBI	-	carbon source
	Only first 10 entries are displayed. Click here to see all.

	Metabolite	Production
[Ref.: #65535]	acetoin ChEBI	no
[Ref.: #65535]	hydrogen sulfide ChEBI	no
[Ref.: #65535]	indole ChEBI	no

	Physiological tests	Metabolite	Voges-Proskauer-test
[Ref.: #65535]		acetoin ChEBI	-

	Enzyme	Enzyme activity	EC number
[Ref.: #65535]	arginine dihydrolase	-	3.5.3.6
[Ref.: #65535]	catalase	-	1.11.1.6
[Ref.: #65535]	cytochrome oxidase	+/-	1.9.3.1
[Ref.: #65535]	DNase	-
[Ref.: #65535]	gelatinase	-
[Ref.: #65535]	lysine decarboxylase	-	4.1.1.18
[Ref.: #65535]	ornithine decarboxylase	-	4.1.1.17
[Ref.: #65535]	tryptophan deaminase	-	4.1.99.1
[Ref.: #65535]	urease	-	3.5.1.5

Fatty acid profile

Metadata FA analysis

Reference

[Ref.: #65535]

Fatty acid	Percentage
C18:1ω7c	61.3
C16:0	19.9
C16:1ω7c / C15:0 iso 2OH	8.4
C12:0 3OH	6.8
C14:0	3.7

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #65535]	Sample type/isolated from	rhizosphere of sand dune coastal plant
[Ref.: #65535]	Geographic location (country and/or sea, region)	Coast of Malpe
[Ref.: #65535]	Country	India
[Ref.: #65535]	Country ISO 3 Code	IND
[Ref.: #65535]	Continent	Asia
[Ref.: #65535]	Geographic location	13.3795°/74.6730°
[Ref.: #65535]	Enrichment culture	modified MY media
[Ref.: #65535]	Enrichment culture composition	containing (per l): 51.3 g NaCl; 9 g MgCl2 . 6H2O; 13 g MgSO4 . 7H2O; 0.2 g CaCl2 . 2H2O; 1.3 g KCl; 0.05 g NaCO3H; 0.15 g NaBr; trace FeCl3 . 6H2O; 10 g glucose; 3 g yeast extract; 3 g malt extract; 5 g proteose-peptone (pH 7.4)
[Ref.: #65535]	Enrichment culture temperature	32 ̊C

[Ref.: #120764]	Sample type/isolated from	Environment, Sand, rhizosphere
[Ref.: #120764]	Geographic location (country and/or sea, region)	Malpe, Karnataka
[Ref.: #120764]	Country	India
[Ref.: #120764]	Country ISO 3 Code	IND
[Ref.: #120764]	Continent	Asia
* marker position based on {}

Isolation sources categories

#Environmental	#Terrestrial	#Coast
#Host Body-Site	#Plant	#Rhizosphere
#Environmental	#Terrestrial	#Sandy
#Host	#Plants	-

What are isolation sources categories?

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

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Biosafety level

Risk group (French classification)

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #65535]	16S rRNA gene seuqence	JQ730736		GenBank

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GC-content

64.8 mol%

thermal denaturation, midpoint method (Tm)

Availability in culture collections External links

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Culture collection no.

LMG 28855, CCM 8737, CIP 111286

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Halomonas montanilacus sp. nov., isolated from hypersaline Lake Pengyanco on the Tibetan Plateau.	Lu H, Xing P, Zhai L, Li H, Wu Q	Int J Syst Evol Microbiol	10.1099/ijsem.0.004109	2020	*
Phylogeny	Halomonas malpeensis sp. nov., isolated from rhizosphere sand of a coastal sand dune plant.	Kampfer P, Rekha PD, Busse HJ, Arun AB, Priyanka P, Glaeser SP	Int J Syst Evol Microbiol	10.1099/ijsem.0.002616	2018	*
Genetics	Genome analysis of a halophilic bacterium Halomonas malpeensis YU-PRIM-29(T) reveals its exopolysaccharide and pigment producing capabilities.	Athmika, Ghate SD, Arun AB, Rao SS, Kumar STA, Kandiyil MK, Saptami K, Rekha PD	Sci Rep	10.1038/s41598-021-81395-1	2021	*

References

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#65535

Peter Kämpfer, P. D. Rekha, Hans-Jürgen Busse, A. B. Arun, P. Priyanka and Stefanie P. Glaeser: Halomonas malpeensis sp. nov., isolated from rhizosphere sand of a coastal sand dune plant. IJSEM 68: 1037 - 1046 2018 ( DOI 10.1099/ijsem.0.002616 , PubMed 29458496 )

#120764 Collection of Institut Pasteur ; Curators of the CIP; CIP 111286
* These data were automatically processed and therefore are not curated

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