Pseudomonas floridensis (LMG 30013, ATCC TSD-90, GEV388)

Name: Pseudomonas floridensis (LMG 30013, ATCC TSD-90, GEV388)
Creator: BacDive
License: http://creativecommons.org/licenses/by/4.0/

BacDive ID 158761

Type strain

Strain Designation GEV388

Culture col. no. LMG 30013 ATCC TSD-90 GEV388 CIP 111601

NCBI tax ID(s) 1958950

Special Collections Literature strains CIP

History CIP <- 2017, S. Timilsina, Florida Univ., Gainesville, Florida, USA: strain GEV388

Links

Pseudomonas floridensis GEV388 is an obligate aerobe, Gram-negative, motile prokaryote that forms circular colonies and was isolated from symptomatic leaf spots in tomato field.

Gram-negative motile rod-shaped colony-forming obligate aerobe genome sequence 16S sequence

Name and taxonomic classification

SI-ID 412704

LMG 30013,ATCC TSD-90,CIP 111601,CFBP 8685

@ref 20215

Last LPSN update 2026-02-09 11:38:24

Domain Bacteria

Phylum Pseudomonadota

Class Gammaproteobacteria

Order Pseudomonadales

Family Pseudomonadaceae

Genus Pseudomonas

Species Pseudomonas floridensis

Full scientific name Pseudomonas floridensis Timilsina et al. 2018

Morphology

Cell morphology

@ref	Gram stain	Cell length	Cell width	Cell shape	Flagellum arrangement	Confidence
65513	negative	1.75-3.6 µm	0.75 µm	rod-shaped	polar
125439	negative					99.9
125438	negative					99.5
125438						90.382
119402	negative			rod-shaped

Colony morphology

@ref	Colony size	Colony shape	Incubation period	Medium used
65513	0.5-1.0 mm	circular	1 day	NA

Culture and growth conditions

Culture medium

@ref	Name	Medium link
65513	Nutrient agar (NA)
65513	King's medium B
119402	CIP Medium 72	Medium recipe at CIP

Culture temp

@ref	Growth	Type	Temperature (°C)	Range
65513	positive	growth	4-37
65513	positive	optimum	26-29	mesophilic

Culture pH

@ref	Ability	Type	PH
65513	positive	growth	5-8

Physiology and metabolism

Oxygen tolerance

@ref	Oxygen tolerance	Confidence
65513	obligate aerobe
125438	aerobe	93.081
125439	obligate aerobe	97.9

Spore formation

@ref	Spore formation	Confidence
65513
125439		99.5

Halophily

@ref	Salt	Growth	Tested relation	Concentration
65513	NaCl	positive	growth	4 %(w/v)

Observation

@ref	Observation
65513	fluorescent cells in King's B agar
65513	yellowish, mucoid biofilm on NA with 5 % sucrose
65513	induces hypersensitive reaction on tobacco

Metabolite utilization

@ref	Chebi-ID	Metabolite	Utilization activity	Kind of utilization tested
65513	16651 ChEBI	(S)-lactate	+	assimilation
65513	30916 ChEBI	2-oxoglutarate	+	assimilation
65513	30089 ChEBI	acetate	+	assimilation
65513	17925 ChEBI	alpha-D-glucose	+	assimilation
65513	16947 ChEBI	citrate	+	assimilation
65513	18333 ChEBI	D-arabitol	+	assimilation
65513	15824 ChEBI	D-fructose	+	assimilation
65513	12936 ChEBI	D-galactose	+	assimilation
65513	18024 ChEBI	D-galacturonic acid	+	assimilation
65513	8391 ChEBI	D-gluconate	+	assimilation
65513	15748 ChEBI	D-glucuronate	+	assimilation
65513	15588 ChEBI	D-malate	+	assimilation
65513	16899 ChEBI	D-mannitol	+	assimilation
65513	16024 ChEBI	D-mannose	+	assimilation
65513	33801 ChEBI	D-saccharate	+	assimilation
65513	17924 ChEBI	D-sorbitol	+	assimilation
65513	16537 ChEBI	galactarate	+	assimilation
65513	16865 ChEBI	gamma-aminobutyric acid	+	assimilation
65513	17754 ChEBI	glycerol	+	assimilation
65513	16977 ChEBI	L-alanine	+	assimilation
65513	29991 ChEBI	L-aspartate	+	assimilation
65513	17464 ChEBI	L-galactonic acid gamma-lactone	+	assimilation
65513	29985 ChEBI	L-glutamate	+	assimilation
65513	15589 ChEBI	L-malate	+	assimilation
65513	18183 ChEBI	L-pyroglutamic acid	+	assimilation
65513	17115 ChEBI	L-serine	+	assimilation
65513	17268 ChEBI	myo-inositol	+	assimilation
65513	17309 ChEBI	pectin	+	hydrolysis
65513	26490 ChEBI	quinate	+	assimilation

Antibiotic resistance

@ref	ChEBI	Metabolite
65513	6472	lincomycin
65513	75273	niaproof
65513	29673	rifamycin sv
65513	75198	tetrazolium blue
65513	75193	tetrazolium violet
65513	45735	troleandomycin
65513	28001	vancomycin

Enzymes

@ref	Value	Activity	Ec
65513	arginine dihydrolase	-	3.5.3.6
65513	catalase	+	1.11.1.6
65513	cytochrome oxidase	-	1.9.3.1

Isolation, sampling and environmental information

Isolation source categories

Cat1	Cat2	Cat3
#Engineered	#Agriculture	#Field
#Host	#Plants	#Herbaceous plants (Grass,Crops)
#Host Body-Site	#Plant	#Leaf (Phyllosphere)
#Infection	#Plant infections	#Spot (Leaf,Stem)

Isolation

@ref	Sample type	Geographic location	Country	Country ISO 3 Code	Continent	Isolation date
65513	symptomatic leaf spots in tomato field	Florida	USA	USA	North America
119402	Environment, Tomato	Florida	United States of America	USA	North America	2011

Interaction and safety

Risk assessment

@ref	Biosafety level	Biosafety level comment
119402	1	Risk group (French classification)

Sequence information

Genome sequences

@ref	Description	Assembly level	INSDC accession	BV-BRC accession	IMG accession	NCBI tax ID	Score
66792	ASM208723v1 assembly for Pseudomonas floridensis GEV388	scaffold	GCA_002087235	1940241.3	2791355582	1958950	20.16

16S sequences

@ref	Description	Accession
65513	Pseudomonas floridensis strain GEV358 16S ribosomal RNA gene, partial sequence	KY614190
65513	Pseudomonas floridensis strain GEV388 16S ribosomal RNA gene, partial sequence	KY614191
65513	Pseudomonas floridensis strain GEV392 16S ribosomal RNA gene, partial sequence	KY614192

GC content

@ref	GC-content (mol%)	Method
65513	59.17	genome sequence analysis

Genome-based predictions

Predictions

Predictions from Deep Learning for Genome Sequence Data. R package version 0.3.1 based on: Pseudomonas floridensis GEV388
NCBI: GCA_002087235

@ref	Trait	Model	Prediction	Confidence in %	In training data
125439	spore_formation	BacteriaNetⓘ	no	99.50	no
125439	motility	BacteriaNetⓘ	yes	88.20	no
125439	gram_stain	BacteriaNetⓘ	negative	99.90	no
125439	oxygen_tolerance	BacteriaNetⓘ	obligate aerobe	97.90	no

Predictions from bacterial phenotypic traits through improved machine learning using high-quality, curated datasets based on: Pseudomonas floridensis GEV388
NCBI: GCA_002087235.1

@ref	Trait	Model	Prediction	Confidence in %	In training data
125438	gram-positive	gram-positiveⓘ	no	99.50	no
125438	anaerobic	anaerobicⓘ	no	99.01	no
125438	aerobic	aerobicⓘ	yes	93.08	no
125438	spore-forming	spore-formingⓘ	no	81.70	no
125438	thermophilic	thermophileⓘ	no	98.50	no
125438	flagellated	motile2+ⓘ	yes	90.38	yes

Literature

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Pseudomonas floridensis sp. nov., a bacterial pathogen isolated from tomato.	Timilsina S, Minsavage GV, Preston J, Newberry EA, Paret ML, Goss EM, Jones JB, Vallad GE	Int J Syst Evol Microbiol	10.1099/ijsem.0.002445	2017
Phylogeny	Pseudomonas ovata sp. nov., Isolated from the Skin of the Tail of Farmed Murray cod (Maccullochella peelii peelii) with a Profound Ulceration.	Rao Q, Liu Y, Chen C, Lin Q, Ren L, Huang M, Tu J, Luo T	Curr Microbiol	10.1007/s00284-019-01729-1	2019

References

#20215	Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )
#65513	Sujan Timilsina, Gerald V. Minsavage, James Preston, Eric A. Newberry, Matthews L. Paret, Erica M. Goss, Jeffrey B. Jones and Gary E. Vallad: Pseudomonas floridensis sp. nov., a bacterial pathogen isolated from tomato. IJSEM 68: 64 - 70 2018 ( DOI 10.1099/ijsem.0.002445 , PubMed 29148362 )
#66792	Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .
#119402	Collection of Institut Pasteur ; Curators of the CIP; CIP 111601
#125438	Julia Koblitz, Lorenz Christian Reimer, Rüdiger Pukall, Jörg Overmann: Predicting bacterial phenotypic traits through improved machine learning using high-quality, curated datasets. 2024 ( DOI 10.1101/2024.08.12.607695 )
#125439	Philipp Münch, René Mreches, Martin Binder, Hüseyin Anil Gündüz, Xiao-Yin To, Alice McHardy: deepG: Deep Learning for Genome Sequence Data. R package version 0.3.1 .
#126262	A. Lissin, I. Schober, J. F. Witte, H. Lüken, A. Podstawka, J. Koblitz, B. Bunk, P. Dawyndt, P. Vandamme, P. de Vos, J. Overmann, L. C. Reimer: StrainInfo—the central database for linked microbial strain identifiers. ( DOI 10.1093/database/baaf059 )

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Pseudomonas floridensis (LMG 30013, ATCC TSD-90, GEV388)

Name and taxonomic classification

Morphology

Cell morphology

Colony morphology

Culture and growth conditions

Culture medium

Culture temp

Culture pH

Physiology and metabolism

Oxygen tolerance

Spore formation

Halophily

Observation

Metabolite utilization

Antibiotic resistance

Enzymes

Isolation, sampling and environmental information

Isolation source categories

Isolation

Interaction and safety

Risk assessment

Sequence information

Genome sequences

Genome browser: GCA_002087235

16S sequences

GC content

Genome-based predictions

Predictions

Literature

Literature

References

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