Name: Flavobacterium luteum KCTC 52572
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 158743

Type strain:

Species: Flavobacterium luteum

Culture col. no.: KCTC 52572, NBRC 112527, IMCC 26026

Strain history: <- Jang-Cheon Cho, Inha Univ.

NCBI tax ID(s): 2026654 (species)

Section

Flavobacterium luteum KCTC 52572 is an aerobe, Gram-negative, rod-shaped bacterium that forms circular colonies and was isolated from lake freshwater sample.

colony-forming
Gram-negative
rod-shaped
aerobe
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Bacteroidota*
	Class	*Flavobacteriia*
	Order	*Flavobacteriales*
	Family	*Flavobacteriaceae*
	Genus	*Flavobacterium*
	Species	**Flavobacterium luteum**
	Full Scientific Name (LPSN)	Flavobacterium luteum Park et al. 2017

Information on morphological and physiological properties Morphology

[Ref.: #65493]	Gram stain	negative
[Ref.: #67771]	Gram stain	negative
[Ref.: #69480]	Gram stain	negative Confidence in %99.996

[Ref.: #65493]	Cell length	1.2-1.5 µm

[Ref.: #65493]	Cell width	0.3-0.5 µm

[Ref.: #65493]	Cell shape	rod-shaped
[Ref.: #67771]	Cell shape	rod-shaped

[Ref.: #65493]	Motility	no
[Ref.: #67771]	Motility	no

[Ref.: #65493]	Colony color	orange
[Ref.: #65493]	Colony shape	circular
[Ref.: #65493]	Incubation period	3 days
[Ref.: #65493]	Cultivation medium used	R2A

[Ref.: #65493]	Pigment production	no
[Ref.: #65493]	Pigment name	felxirubin

Information on culture and growth conditions Culture and growth conditions

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Culture medium

Reasoner's 2A agar (R2A)

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Culture medium growth

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Culture medium

Marine agar (MA)

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Culture medium growth

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Culture medium

Nutrient agar (NA)

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Culture medium growth

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Culture medium

PCA

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Culture medium growth

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Culture medium

Trypticase Soy Agar (TSA)

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Culture medium growth

		Temperature
[Ref.: #65493]	growth	4-37 °C
[Ref.: #65493]	optimum	20 °C
[Ref.: #67771]	growth	25 °C

[Ref.: #65493]	Temperature range	psychrophilic
[Ref.: #67771]	Temperature range	mesophilic

	pH	Kind of pH		pH
[Ref.: #65493]			optimum	7
[Ref.: #65493]			growth	6-8

Information on physiology and metabolism Physiology and metabolism

[Ref.: #65493]	Oxygen tolerance	aerobe
[Ref.: #67771]	Oxygen tolerance	aerobe

[Ref.: #69480]	Ability of spore formation	no Confidence in %100
[Ref.: #69481]	Ability of spore formation	no Confidence in %100

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Halophily / tolerance level

non-halophilic

	Halophily	Salt	Tested relation		Salt conc.
[Ref.: #65493]		NaCl		growth	0-1	%
[Ref.: #65493]		NaCl		optimum	0	%

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Observation

quinones: MK-6

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #65493]	alpha-cyclodextrin ChEBI	+	oxidation
[Ref.: #65493]	alpha-D-glucose ChEBI	+	oxidation
[Ref.: #65493]	carboxymethylcellulose ChEBI	-	hydrolysis
[Ref.: #65493]	casein	-	hydrolysis
[Ref.: #65493]	chitin ChEBI	-	hydrolysis
[Ref.: #65493]	D-galactose ChEBI	+	oxidation
[Ref.: #65493]	D-mannose ChEBI	+	oxidation
[Ref.: #65493]	dextrin ChEBI	+	oxidation
[Ref.: #65493]	dna ChEBI	-	hydrolysis
[Ref.: #65493]	esculin ChEBI	+	hydrolysis
[Ref.: #65493]	gelatin ChEBI	-	hydrolysis
[Ref.: #65493]	glucose ChEBI	-	fermentation
[Ref.: #65493]	glycogen ChEBI	+	oxidation
[Ref.: #65493]	inosine ChEBI	+	oxidation
[Ref.: #65493]	L-ornithine ChEBI	+	oxidation
[Ref.: #65493]	L-proline ChEBI	+	oxidation
[Ref.: #65493]	maltose ChEBI	+	oxidation
[Ref.: #65493]	monomethyl succinate ChEBI	+	oxidation
[Ref.: #65493]	nitrate ChEBI	-	reduction
[Ref.: #65493]	starch ChEBI	+	hydrolysis
[Ref.: #65493]	sucrose ChEBI	+	oxidation
[Ref.: #65493]	thymidine ChEBI	+	oxidation
[Ref.: #65493]	tween 20 ChEBI	-	hydrolysis
[Ref.: #65493]	tween 80 ChEBI	-	hydrolysis
[Ref.: #65493]	urea ChEBI	+	hydrolysis
[Ref.: #65493]	uridine ChEBI	+	oxidation
	Only first 10 entries are displayed. Click here to see all.

	Metabolite production	Metabolite	Production
[Ref.: #65493]		indole ChEBI	no

	Enzyme	Enzyme activity	EC number
[Ref.: #65493]	acid phosphatase	+	3.1.3.2
[Ref.: #65493]	alkaline phosphatase	+	3.1.3.1
[Ref.: #65493]	alpha-chymotrypsin	-	3.4.21.1
[Ref.: #65493]	alpha-fucosidase	-	3.2.1.51
[Ref.: #65493]	alpha-galactosidase	-	3.2.1.22
[Ref.: #65493]	alpha-glucosidase	-	3.2.1.20
[Ref.: #65493]	alpha-mannosidase	-	3.2.1.24
[Ref.: #65493]	arginine dihydrolase	-	3.5.3.6
[Ref.: #65493]	beta-galactosidase	-	3.2.1.23
[Ref.: #65493]	beta-glucosidase	-	3.2.1.21
[Ref.: #65493]	beta-glucuronidase	-	3.2.1.31
[Ref.: #65493]	catalase	+	1.11.1.6
[Ref.: #65493]	cystine arylamidase	-	3.4.11.3
[Ref.: #65493]	cytochrome oxidase	+	1.9.3.1
[Ref.: #65493]	esterase (C 4)	+
[Ref.: #65493]	esterase Lipase (C 8)	+
[Ref.: #65493]	leucine arylamidase	+	3.4.11.1
[Ref.: #65493]	lipase (C 14)	-
[Ref.: #65493]	N-acetyl-beta-glucosaminidase	-	3.2.1.52
[Ref.: #65493]	naphthol-AS-BI-phosphohydrolase	+
[Ref.: #65493]	trypsin	+	3.4.21.4
[Ref.: #65493]	valine arylamidase	+
	Only first 10 entries are displayed. Click here to see all.

Fatty acid profile

Metadata FA analysis

Reference

[Ref.: #65493]

Fatty acid	Percentage
C16:1ω7c / C16:1ω6c	24.6
C15:0 iso	16.8
C15:0 anteiso	15.3
C15:1 iso G	8.2
C15:1ω6c	5.9
C15:0 iso 3OH	4.4
C16:0	3.6
C16:0 iso	3
C17:0 iso 3OH	2.9
C16:0 10-methyl / iso-C17:ω9c	2.7
C17:1ω6c	2.1
C16:1 iso H	2
C16:0 iso 3OH	1.5
C17:1 anteiso ω9c	1.2
C14:0	1.1

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #65493]	Sample type/isolated from	lake freshwater sample
[Ref.: #65493]	Geographic location (country and/or sea, region)	Lake Soyang, Chuncheon
[Ref.: #65493]	Country	Republic of Korea
[Ref.: #65493]	Country ISO 3 Code	KOR
[Ref.: #65493]	Continent	Asia
[Ref.: #65493]	Geographic location	37.9475°/127.8190°
[Ref.: #65493]	Enrichment culture	Supplemented autoclaved lake water
[Ref.: #65493]	Enrichment culture composition	Autoclaved lake water supplemented with NH4Cl (10 µM), KH2PO4 (10 µM), pyruvic acid (50 µM), each 5 µM of D-glucose, D-ribose, N-acetyl-D-glucosamine and methyl alcohol, and a 1:10000 dilution of a vitamin mixture.
[Ref.: #65493]	Enrichment culture duration	4 weeks
[Ref.: #65493]	Enrichment culture temperature	15 ̊C
[Ref.: #65493]	Isolation/enrichment procedure	High-throughput culturing method based on dilution-to-extinction. The freshwater sample was diuluted to 5 cell/ml incubated in microtiterplates. Subsequently, growth positive cultures were spread onto R2A agar and incubated at 15 °C for 7 days.

[Ref.: #67771]	Sample type/isolated from	From fresh water
[Ref.: #67771]	Geographic location (country and/or sea, region)	Soyang Lake, Chuncheon
[Ref.: #67771]	Country	Republic of Korea
[Ref.: #67771]	Country ISO 3 Code	KOR
[Ref.: #67771]	Continent	Asia
[Ref.: #67771]	Geographic location	37.9475°/127.8190°
* marker position based on {}

Isolation sources categories

#Environmental	#Aquatic	#Freshwater
#Environmental	#Aquatic	#Lake (large)

What are isolation sources categories?

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #65493]	16S rRNA gene sequence	KX505860		GenBank

Genome sequence information:

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #66792]	Flavobacterium luteum NBRC 112527	GCA_008806775	contig	GenBank	2026654 tax ID	*
[Ref.: #66792]	Flavobacterium luteum strain NBRC 112527	2026654.3	wgs	PATRIC	2026654 tax ID	*
[Ref.: #66792]	Flavobacterium luteum NBRC 112527	2906829834	draft	JGI IMG/M	2026654 tax ID	*

[Ref.: #65493]	GC-content	33.7 mol%	thermal denaturation, midpoint method (Tm)
[Ref.: #67771]	GC-content	33.7 mol%

Data predicted using genome information as a basis Genome-based predictions

	Trait	Prediction	Confidence in %	In training data
	Predictions from GenomeNet Sporulation v. 1 based on: Flavobacterium luteum strain NBRC 112527 PATRIC: 2026654.3
[Ref.: #69481]	spore-forming	no	100	no

	Trait	Prediction	Confidence in %	In training data
	Predictions from Diaspora project based on: Flavobacterium luteum strain NBRC 112527 PATRIC: 2026654.3
[Ref.: #69480]	motile	no	93.839	no
[Ref.: #69480]	gram-positive	no	98.582	yes
[Ref.: #69480]	anaerobic	no	98.78	yes
[Ref.: #69480]	aerobic	yes	82.84	no
[Ref.: #69480]	halophile	no	95.301	yes
[Ref.: #69480]	spore-forming	no	97.112	no
[Ref.: #69480]	glucose-util	yes	78.575	no
[Ref.: #69480]	flagellated	no	96.809	no
[Ref.: #69480]	thermophile	no	91.913	no
[Ref.: #69480]	glucose-ferment	no	91.042	yes

Availability in culture collections External links

[Ref.: #65493]

Culture collection no.

KCTC 52572, NBRC 112527, IMCC 26026

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Flavobacterium chuncheonense sp. nov. and Flavobacterium luteum sp. nov., isolated from a freshwater lake.	Park M, Nam GG, Kim S, Jeon HT, Joung Y, Cho JC	Int J Syst Evol Microbiol	10.1099/ijsem.0.002304	2017	*
Phylogeny	Flavobacterium psychrotolerans sp. nov., a psychrotolerant bacterium isolated from Renlongba glacier on the Tibetan Plateau.	Yang LL, Liu Q, Liu HC, Zhou YG, Xin YH	Int J Syst Evol Microbiol	10.1099/ijsem.0.003868	2020	*

References

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#65493

Miri Park, Gi Gyun Nam, Suhyun Kim, Hyoung Tae Jeon, Yochan Joung and Jang-Cheon Cho: Flavobacterium chuncheonense sp. nov. and Flavobacterium luteum sp. nov., isolated from a freshwater lake. IJSEM 67: 4409 - 4415 2017 ( DOI 10.1099/ijsem.0.002304 , PubMed 28920853 )

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#67771 Korean Collection for Type Cultures (KCTC) ; Curators of the KCTC;

#69480

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#69481

Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch: Predictions based on the model GenomeNet Sporulation v. 1 . ( DOI 10.21203/rs.3.rs-2527258/v1 )

* These data were automatically processed and therefore are not curated

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Flavobacterium luteum strain NBRC 112527

Flavobacterium luteum strain NBRC 112527

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