Name: Roseovarius ramblicola D15
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 158737

Type strain:

Species: Roseovarius ramblicola

Strain Designation: D15

Culture col. no.: CECT 9424, LMG 30322

NCBI tax ID(s): 2022336 (species)

Section

Roseovarius ramblicola D15 is a rod-shaped bacterium that forms circular colonies and was isolated from a sample of soil taken from Rambla Salada , a hypersaline rambla .

colony-forming
rod-shaped
16S sequence
Bacteria

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	07-12-2022 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Pseudomonadota*
	Class	*Alphaproteobacteria*
	Order	*Rhodobacterales*
	Family	Rhodobacteraceae
	Genus	*Roseovarius*
	Species	**Roseovarius ramblicola**
	Full Scientific Name (PNU)	Roseovarius ramblicola Castro et al. 2018

Information on morphological and physiological properties Morphology

[Ref.: #65488]	Gram stain	negative
[Ref.: #65488]	Cell length	2.1-3.2 µm
[Ref.: #65488]	Cell width	0.7-1.2 µm
[Ref.: #65488]	Cell shape	ovoid-shaped
[Ref.: #65488]	Motility	no

[Ref.: #65488]	Cell shape	rod-shaped

[Ref.: #65488]	Colony shape	circular
[Ref.: #65488]	Cultivation medium used	marine agar, Reasoner's 2A agar

Information on culture and growth conditions Culture and growth conditions

[Ref.: #65488]

Culture medium

marine agar, Reasoner's 2A agar

[Ref.: #65488]

Culture medium growth

	Temperatures	Kind of temperature		Temperature
[Ref.: #65488]			growth	5-30 °C
[Ref.: #65488]			optimum	28 °C

[Ref.: #65488]

Temperature range

mesophilic

	pH	Kind of pH		pH	pH range
[Ref.: #65488]			optimum	7
[Ref.: #65488]			growth	6-9	alkaliphile

Information on physiology and metabolism Physiology and metabolism

	Halophily	Salt	Tested relation		Salt conc.
[Ref.: #65488]		NaCl		growth	0.5-10	%(w/v)
[Ref.: #65488]		NaCl		optimum	3	%(w/v)

[Ref.: #65488]	Observation	reproduce by budding or asymmetrical division
[Ref.: #65488]	Observation	growth pattern is uniform in a liquid medium
[Ref.: #65488]	Observation	only detected lipoquinone is ubiquinone with ten isoprene units (Q10)
[Ref.: #65488]	Observation	polar lipids contain phosphatidylcholine, phosphatidylethanolamine, phosphatidylglycerol, aminolipid and three unidentified polar lipids

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #65488]	adipate ChEBI	-	assimilation
[Ref.: #65488]	amygdalin ChEBI	-	builds acid from
[Ref.: #65488]	arbutin ChEBI	-	builds acid from
[Ref.: #65488]	arginine ChEBI	-	hydrolysis
[Ref.: #65488]	cellobiose ChEBI	-	builds acid from
[Ref.: #65488]	D-arabinose ChEBI	-	builds acid from
[Ref.: #65488]	D-arabitol ChEBI	-	builds acid from
[Ref.: #65488]	D-fructose ChEBI	-	builds acid from
[Ref.: #65488]	D-fucose ChEBI	-	builds acid from
[Ref.: #65488]	D-galactose ChEBI	-	builds acid from
[Ref.: #65488]	D-glucose ChEBI	-	assimilation
[Ref.: #65488]	D-glucose ChEBI	-	builds acid from
[Ref.: #65488]	D-lyxose ChEBI	-	builds acid from
[Ref.: #65488]	D-mannitol ChEBI	-	builds acid from
[Ref.: #65488]	D-mannose ChEBI	-	builds acid from
[Ref.: #65488]	D-ribose ChEBI	-	builds acid from
[Ref.: #65488]	D-sorbitol ChEBI	-	builds acid from
[Ref.: #65488]	D-tagatose ChEBI	-	builds acid from
[Ref.: #65488]	D-xylose ChEBI	-	builds acid from
[Ref.: #65488]	decanoate ChEBI	-	assimilation
[Ref.: #65488]	erythritol ChEBI	-	builds acid from
[Ref.: #65488]	esculin ChEBI	-	hydrolysis
[Ref.: #65488]	esculin ferric citrate	-	builds acid from
[Ref.: #65488]	galactitol ChEBI	-	builds acid from
[Ref.: #65488]	gelatin ChEBI	-	hydrolysis
[Ref.: #65488]	gentiobiose ChEBI	-	builds acid from
[Ref.: #65488]	gluconate ChEBI	-	assimilation
[Ref.: #65488]	glycerol ChEBI	-	builds acid from
[Ref.: #65488]	glycogen ChEBI	-	builds acid from
[Ref.: #65488]	inulin ChEBI	-	builds acid from
[Ref.: #65488]	L-arabinose ChEBI	-	assimilation
[Ref.: #65488]	L-arabinose ChEBI	-	builds acid from
[Ref.: #65488]	L-arabitol ChEBI	-	builds acid from
[Ref.: #65488]	L-fucose ChEBI	-	builds acid from
[Ref.: #65488]	L-rhamnose ChEBI	-	builds acid from
[Ref.: #65488]	L-sorbose ChEBI	-	builds acid from
[Ref.: #65488]	L-xylose ChEBI	-	builds acid from
[Ref.: #65488]	lactose ChEBI	-	builds acid from
[Ref.: #65488]	malate ChEBI	-	assimilation
[Ref.: #65488]	maltose ChEBI	-	assimilation
[Ref.: #65488]	maltose ChEBI	-	builds acid from
[Ref.: #65488]	mannose ChEBI	-	assimilation
[Ref.: #65488]	melezitose ChEBI	-	builds acid from
[Ref.: #65488]	melibiose ChEBI	-	builds acid from
[Ref.: #65488]	methyl alpha-D-glucopyranoside ChEBI	-	builds acid from
[Ref.: #65488]	methyl alpha-D-mannoside ChEBI	-	builds acid from
[Ref.: #65488]	methyl beta-D-xylopyranoside ChEBI	-	builds acid from
[Ref.: #65488]	myo-inositol ChEBI	-	builds acid from
[Ref.: #65488]	N-acetylglucosamine ChEBI	-	assimilation
[Ref.: #65488]	N-acetylglucosamine ChEBI	-	builds acid from
[Ref.: #65488]	nitrate ChEBI	-	reduction
[Ref.: #65488]	nitrite ChEBI	-	reduction
[Ref.: #65488]	phenylacetate ChEBI	-	assimilation
[Ref.: #65488]	potassium 2-dehydro-D-gluconate	-	builds acid from
[Ref.: #65488]	potassium 5-dehydro-D-gluconate	-	builds acid from
[Ref.: #65488]	potassium gluconate ChEBI	-	builds acid from
[Ref.: #65488]	raffinose ChEBI	-	builds acid from
[Ref.: #65488]	ribitol ChEBI	-	builds acid from
[Ref.: #65488]	salicin ChEBI	-	builds acid from
[Ref.: #65488]	sodium citrate ChEBI	-	assimilation
[Ref.: #65488]	starch ChEBI	-	builds acid from
[Ref.: #65488]	sucrose ChEBI	-	builds acid from
[Ref.: #65488]	trehalose ChEBI	-	builds acid from
[Ref.: #65488]	turanose ChEBI	-	builds acid from
[Ref.: #65488]	xylitol ChEBI	-	builds acid from
	Only first 10 entries are displayed. Click here to see all.

	Metabolite	Antibiotic sensitivity	Antibiotic resistance
[Ref.: #65488]	amikacin ChEBI		yes
[Ref.: #65488]	amoxicillin ChEBI	yes
[Ref.: #65488]	ampicillin ChEBI	yes
[Ref.: #65488]	azithromycin ChEBI	yes
[Ref.: #65488]	aztreonam ChEBI	yes
[Ref.: #65488]	bacitracin ChEBI	yes
[Ref.: #65488]	carbenicillin ChEBI	yes
[Ref.: #65488]	cefalotin ChEBI	yes
[Ref.: #65488]	cefotaxime ChEBI	yes
[Ref.: #65488]	cefuroxime ChEBI	yes
[Ref.: #65488]	chloramphenicol ChEBI	yes
[Ref.: #65488]	ciprofloxacin ChEBI		yes
[Ref.: #65488]	erythromycin ChEBI	yes
[Ref.: #65488]	gentamicin ChEBI		yes
[Ref.: #65488]	nalidixic acid ChEBI		yes
[Ref.: #65488]	neomycin ChEBI		yes
[Ref.: #65488]	norfloxacin ChEBI	yes
[Ref.: #65488]	penicillin ChEBI	yes
[Ref.: #65488]	polymyxin b ChEBI		yes
[Ref.: #65488]	rifampicin ChEBI	yes
[Ref.: #65488]	streptomycin ChEBI	yes
[Ref.: #65488]	sulfonamide ChEBI	yes
[Ref.: #65488]	tetracycline ChEBI		yes
[Ref.: #65488]	tobramycin ChEBI	yes
	Only first 10 entries are displayed. Click here to see all.

	Group ID of combined antibiotics	Metabolite	Antibiotic sensitivity
[Ref.: #65488]	11	amoxicillin ChEBI	yes
[Ref.: #65488]	11	clavulanic acid ChEBI	yes
[Ref.: #65488]	26	sulfamethoxazole ChEBI	yes
[Ref.: #65488]	26	trimethoprim ChEBI	yes

	Metabolite production	Metabolite
[Ref.: #65488]		indole ChEBI

	Enzyme	Enzyme activity	EC number
[Ref.: #65488]	alkaline phosphatase	-	3.1.3.1
[Ref.: #65488]	alpha-chymotrypsin	-	3.4.21.1
[Ref.: #65488]	alpha-fucosidase	-	3.2.1.51
[Ref.: #65488]	alpha-galactosidase	-	3.2.1.22
[Ref.: #65488]	alpha-glucosidase	-	3.2.1.20
[Ref.: #65488]	alpha-mannosidase	-	3.2.1.24
[Ref.: #65488]	beta-galactosidase	-	3.2.1.23
[Ref.: #65488]	beta-glucosidase	-	3.2.1.21
[Ref.: #65488]	beta-glucuronidase	-	3.2.1.31
[Ref.: #65488]	catalase	+	1.11.1.6
[Ref.: #65488]	cystine arylamidase	-	3.4.11.3
[Ref.: #65488]	cytochrome oxidase	+	1.9.3.1
[Ref.: #65488]	esterase (C 4)	-
[Ref.: #65488]	esterase Lipase (C 8)	-
[Ref.: #65488]	leucine arylamidase	+	3.4.11.1
[Ref.: #65488]	lipase (C 14)	-
[Ref.: #65488]	N-acetyl-beta-glucosaminidase	-	3.2.1.52
[Ref.: #65488]	naphthol-AS-BI-phosphohydrolase	-
[Ref.: #65488]	trypsin	-	3.4.21.4
[Ref.: #65488]	urease	-	3.5.1.5
[Ref.: #65488]	valine arylamidase	-
	Only first 10 entries are displayed. Click here to see all.

Fatty acid profile

Metadata FA analysis

Reference

[Ref.: #65488]

Fatty acid	Percentage
C18:1ω7c	61.5
C16:0	11.9
C12:0	7.1
C12:1 3OH	3.6
C16:0 2OH	2.5
C18:1ω7c 11-methyl	2.3
C18:0	1.8
Cyclo-C19:0ω8c	0.9
C12:0 2OH	0.9
C16:1ω7c/C16:1ω6c	0.8
C18:1ω9c	0.7

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #65488]	Sample type/isolated from	a sample of soil taken from Rambla Salada (Murcia), a hypersaline rambla (a steep-sided river bed, normally dry but subject to flash flooding)
[Ref.: #65488]	Country	Spain
[Ref.: #65488]	Country ISO 3 Code	ESP
[Ref.: #65488]	Continent	Europe
[Ref.: #65488]	Geographic location	38.1241°/-1.1170°
[Ref.: #65488]	Enrichment culture composition	S3, a lownutrient medium supplemented with 3 % (w/v) sea-salt solution
[Ref.: #65488]	Enrichment culture duration	30 days
[Ref.: #65488]	Enrichment culture temperature	25 ̊C
[Ref.: #65488]	Isolation/enrichment procedure	serial dilutions from 1g soil were prepared,previously sonicated for 30s, in 10ml supplemented S3 medium. The number of micro-organisms in each dilution was determined using a Petroff Hausser counting chamber using methylene blue as contrast. A 48-well microtiter plate, containing 490µl supplemented S3 medium, was inoculated with 10µl dilution containing 100 bacteria per millilitre (approximately 1 bacterium per well).
* marker position based on {}

Isolation sources categories

#Environmental	#Aquatic	#River (Creek)
#Environmental	#Terrestrial	#Soil
#Condition	#Saline	-

What are isolation sources categories?

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #65488]	16S rRNA gene sequence	MF527111		GenBank

[Ref.: #65488]

GC-content

63 mol%

thermal denaturation, midpoint method (Tm)

Availability in culture collections External links

[Ref.: #65488]

Culture collection no.

CECT 9424, LMG 30322

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Roseovarius ramblicola sp. nov., a moderately halophilic bacterium isolated from saline soil in Spain.	Castro DJ, Cerezo I, Sampedro I, Martinez-Checa F	Int J Syst Evol Microbiol	10.1099/ijsem.0.002744	2018	*

References

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#65488

David J. Castro, Isabel Cerezo, Inmaculada Sampedro, Fernando Martínez-Checa: Roseovarius ramblicola sp. nov., a moderately halophilic bacterium isolated from saline soil in Spain. IJSEM 68: 1839 - 1844 2018 ( DOI 10.1099/ijsem.0.002744 , PubMed 29664362 )

* These data were automatically processed and therefore are not curated

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