Name: Roseovarius ramblicola D15
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 158737

Type strain:

Species: Roseovarius ramblicola

Strain Designation: D15

Culture col. no.: CECT 9424, LMG 30322

NCBI tax ID(s): 2022336 (species)

Section

Roseovarius ramblicola D15 is a rod-shaped bacterium that forms circular colonies and was isolated from a sample of soil taken from Rambla Salada , a hypersaline rambla .

colony-forming
rod-shaped
16S sequence
Bacteria

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Pseudomonadota*
	Class	*Alphaproteobacteria*
	Order	*Rhodobacterales*
	Family	*Roseobacteraceae*
	Genus	*Roseovarius*
	Species	**Roseovarius ramblicola**
	Full Scientific Name (LPSN)	Roseovarius ramblicola Castro et al. 2018

Information on morphological and physiological properties Morphology

[Ref.: #65488]	Gram stain	negative

[Ref.: #65488]	Cell length	2.1-3.2 µm

[Ref.: #65488]	Cell width	0.7-1.2 µm

[Ref.: #65488]	Cell shape	ovoid-shaped
[Ref.: #65488]	Cell shape	rod-shaped

[Ref.: #65488]	Motility	no

[Ref.: #65488]	Colony shape	circular
[Ref.: #65488]	Cultivation medium used	marine agar, Reasoner's 2A agar

Information on culture and growth conditions Culture and growth conditions

[Ref.: #65488]

Culture medium

marine agar, Reasoner's 2A agar

[Ref.: #65488]

Culture medium growth

	Temperatures	Kind of temperature		Temperature
[Ref.: #65488]			growth	5-30 °C
[Ref.: #65488]			optimum	28 °C

[Ref.: #65488]

Temperature range

mesophilic

	pH	Kind of pH		pH
[Ref.: #65488]			optimum	7
[Ref.: #65488]			growth	6-9

Information on physiology and metabolism Physiology and metabolism

	Halophily	Salt	Tested relation		Salt conc.
[Ref.: #65488]		NaCl		growth	0.5-10	%(w/v)
[Ref.: #65488]		NaCl		optimum	3	%(w/v)

[Ref.: #65488]	Observation	reproduce by budding or asymmetrical division
[Ref.: #65488]	Observation	growth pattern is uniform in a liquid medium
[Ref.: #65488]	Observation	only detected lipoquinone is ubiquinone with ten isoprene units (Q10)
[Ref.: #65488]	Observation	polar lipids contain phosphatidylcholine, phosphatidylethanolamine, phosphatidylglycerol, aminolipid and three unidentified polar lipids

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #65488]	adipate ChEBI	-	assimilation
[Ref.: #65488]	amygdalin ChEBI	-	builds acid from
[Ref.: #65488]	arbutin ChEBI	-	builds acid from
[Ref.: #65488]	arginine ChEBI	-	hydrolysis
[Ref.: #65488]	cellobiose ChEBI	-	builds acid from
[Ref.: #65488]	D-arabinose ChEBI	-	builds acid from
[Ref.: #65488]	D-arabitol ChEBI	-	builds acid from
[Ref.: #65488]	D-fructose ChEBI	-	builds acid from
[Ref.: #65488]	D-fucose ChEBI	-	builds acid from
[Ref.: #65488]	D-galactose ChEBI	-	builds acid from
[Ref.: #65488]	D-glucose ChEBI	-	assimilation
[Ref.: #65488]	D-glucose ChEBI	-	builds acid from
[Ref.: #65488]	D-lyxose ChEBI	-	builds acid from
[Ref.: #65488]	D-mannitol ChEBI	-	builds acid from
[Ref.: #65488]	D-mannose ChEBI	-	builds acid from
[Ref.: #65488]	D-ribose ChEBI	-	builds acid from
[Ref.: #65488]	D-sorbitol ChEBI	-	builds acid from
[Ref.: #65488]	D-tagatose ChEBI	-	builds acid from
[Ref.: #65488]	D-xylose ChEBI	-	builds acid from
[Ref.: #65488]	decanoate ChEBI	-	assimilation
[Ref.: #65488]	erythritol ChEBI	-	builds acid from
[Ref.: #65488]	esculin ChEBI	-	hydrolysis
[Ref.: #65488]	esculin ferric citrate	-	builds acid from
[Ref.: #65488]	galactitol ChEBI	-	builds acid from
[Ref.: #65488]	gelatin ChEBI	-	hydrolysis
[Ref.: #65488]	gentiobiose ChEBI	-	builds acid from
[Ref.: #65488]	gluconate ChEBI	-	assimilation
[Ref.: #65488]	glycerol ChEBI	-	builds acid from
[Ref.: #65488]	glycogen ChEBI	-	builds acid from
[Ref.: #65488]	inulin ChEBI	-	builds acid from
[Ref.: #65488]	L-arabinose ChEBI	-	assimilation
[Ref.: #65488]	L-arabinose ChEBI	-	builds acid from
[Ref.: #65488]	L-arabitol ChEBI	-	builds acid from
[Ref.: #65488]	L-fucose ChEBI	-	builds acid from
[Ref.: #65488]	L-rhamnose ChEBI	-	builds acid from
[Ref.: #65488]	L-sorbose ChEBI	-	builds acid from
[Ref.: #65488]	L-xylose ChEBI	-	builds acid from
[Ref.: #65488]	lactose ChEBI	-	builds acid from
[Ref.: #65488]	malate ChEBI	-	assimilation
[Ref.: #65488]	maltose ChEBI	-	assimilation
[Ref.: #65488]	maltose ChEBI	-	builds acid from
[Ref.: #65488]	mannose ChEBI	-	assimilation
[Ref.: #65488]	melezitose ChEBI	-	builds acid from
[Ref.: #65488]	melibiose ChEBI	-	builds acid from
[Ref.: #65488]	methyl alpha-D-glucopyranoside ChEBI	-	builds acid from
[Ref.: #65488]	methyl alpha-D-mannoside ChEBI	-	builds acid from
[Ref.: #65488]	methyl beta-D-xylopyranoside ChEBI	-	builds acid from
[Ref.: #65488]	myo-inositol ChEBI	-	builds acid from
[Ref.: #65488]	N-acetylglucosamine ChEBI	-	assimilation
[Ref.: #65488]	N-acetylglucosamine ChEBI	-	builds acid from
[Ref.: #65488]	nitrate ChEBI	-	reduction
[Ref.: #65488]	nitrite ChEBI	-	reduction
[Ref.: #65488]	phenylacetate ChEBI	-	assimilation
[Ref.: #65488]	potassium 2-dehydro-D-gluconate	-	builds acid from
[Ref.: #65488]	potassium 5-dehydro-D-gluconate	-	builds acid from
[Ref.: #65488]	potassium gluconate ChEBI	-	builds acid from
[Ref.: #65488]	raffinose ChEBI	-	builds acid from
[Ref.: #65488]	ribitol ChEBI	-	builds acid from
[Ref.: #65488]	salicin ChEBI	-	builds acid from
[Ref.: #65488]	sodium citrate ChEBI	-	assimilation
[Ref.: #65488]	starch ChEBI	-	builds acid from
[Ref.: #65488]	sucrose ChEBI	-	builds acid from
[Ref.: #65488]	trehalose ChEBI	-	builds acid from
[Ref.: #65488]	turanose ChEBI	-	builds acid from
[Ref.: #65488]	xylitol ChEBI	-	builds acid from
	Only first 10 entries are displayed. Click here to see all.

	Metabolite	Antibiotic sensitivity	Antibiotic resistance
[Ref.: #65488]	amikacin ChEBI		yes
[Ref.: #65488]	amoxicillin ChEBI	yes
[Ref.: #65488]	ampicillin ChEBI	yes
[Ref.: #65488]	azithromycin ChEBI	yes
[Ref.: #65488]	aztreonam ChEBI	yes
[Ref.: #65488]	bacitracin ChEBI	yes
[Ref.: #65488]	carbenicillin ChEBI	yes
[Ref.: #65488]	cefalotin ChEBI	yes
[Ref.: #65488]	cefotaxime ChEBI	yes
[Ref.: #65488]	cefuroxime ChEBI	yes
[Ref.: #65488]	chloramphenicol ChEBI	yes
[Ref.: #65488]	ciprofloxacin ChEBI		yes
[Ref.: #65488]	erythromycin ChEBI	yes
[Ref.: #65488]	gentamicin ChEBI		yes
[Ref.: #65488]	nalidixic acid ChEBI		yes
[Ref.: #65488]	neomycin ChEBI		yes
[Ref.: #65488]	norfloxacin ChEBI	yes
[Ref.: #65488]	penicillin ChEBI	yes
[Ref.: #65488]	polymyxin b ChEBI		yes
[Ref.: #65488]	rifampicin ChEBI	yes
[Ref.: #65488]	streptomycin ChEBI	yes
[Ref.: #65488]	sulfonamide ChEBI	yes
[Ref.: #65488]	tetracycline ChEBI		yes
[Ref.: #65488]	tobramycin ChEBI	yes
	Only first 10 entries are displayed. Click here to see all.

	Group ID of combined antibiotics	Metabolite	Antibiotic sensitivity
[Ref.: #65488]	11	amoxicillin ChEBI	yes
[Ref.: #65488]	11	clavulanic acid ChEBI	yes
[Ref.: #65488]	26	sulfamethoxazole ChEBI	yes
[Ref.: #65488]	26	trimethoprim ChEBI	yes

	Metabolite production	Metabolite	Production
[Ref.: #65488]		indole ChEBI	no

	Enzyme	Enzyme activity	EC number
[Ref.: #65488]	alkaline phosphatase	-	3.1.3.1
[Ref.: #65488]	alpha-chymotrypsin	-	3.4.21.1
[Ref.: #65488]	alpha-fucosidase	-	3.2.1.51
[Ref.: #65488]	alpha-galactosidase	-	3.2.1.22
[Ref.: #65488]	alpha-glucosidase	-	3.2.1.20
[Ref.: #65488]	alpha-mannosidase	-	3.2.1.24
[Ref.: #65488]	beta-galactosidase	-	3.2.1.23
[Ref.: #65488]	beta-glucosidase	-	3.2.1.21
[Ref.: #65488]	beta-glucuronidase	-	3.2.1.31
[Ref.: #65488]	catalase	+	1.11.1.6
[Ref.: #65488]	cystine arylamidase	-	3.4.11.3
[Ref.: #65488]	cytochrome oxidase	+	1.9.3.1
[Ref.: #65488]	esterase (C 4)	-
[Ref.: #65488]	esterase Lipase (C 8)	-
[Ref.: #65488]	leucine arylamidase	+	3.4.11.1
[Ref.: #65488]	lipase (C 14)	-
[Ref.: #65488]	N-acetyl-beta-glucosaminidase	-	3.2.1.52
[Ref.: #65488]	naphthol-AS-BI-phosphohydrolase	-
[Ref.: #65488]	trypsin	-	3.4.21.4
[Ref.: #65488]	urease	-	3.5.1.5
[Ref.: #65488]	valine arylamidase	-
	Only first 10 entries are displayed. Click here to see all.

Fatty acid profile

Metadata FA analysis

Reference

[Ref.: #65488]

Fatty acid	Percentage
C18:1ω7c	61.5
C16:0	11.9
C12:0	7.1
C12:1 3OH	3.6
C16:0 2OH	2.5
C18:1ω7c 11-methyl	2.3
C18:0	1.8
Cyclo-C19:0ω8c	0.9
C12:0 2OH	0.9
C16:1ω7c/C16:1ω6c	0.8
C18:1ω9c	0.7

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #65488]	Sample type/isolated from	a sample of soil taken from Rambla Salada (Murcia), a hypersaline rambla (a steep-sided river bed, normally dry but subject to flash flooding)
[Ref.: #65488]	Country	Spain
[Ref.: #65488]	Country ISO 3 Code	ESP
[Ref.: #65488]	Continent	Europe
[Ref.: #65488]	Geographic location	38.1241°/-1.1170°
[Ref.: #65488]	Enrichment culture composition	S3, a lownutrient medium supplemented with 3 % (w/v) sea-salt solution
[Ref.: #65488]	Enrichment culture duration	30 days
[Ref.: #65488]	Enrichment culture temperature	25 ̊C
[Ref.: #65488]	Isolation/enrichment procedure	serial dilutions from 1g soil were prepared,previously sonicated for 30s, in 10ml supplemented S3 medium. The number of micro-organisms in each dilution was determined using a Petroff Hausser counting chamber using methylene blue as contrast. A 48-well microtiter plate, containing 490µl supplemented S3 medium, was inoculated with 10µl dilution containing 100 bacteria per millilitre (approximately 1 bacterium per well).
* marker position based on {}

Isolation sources categories

#Environmental	#Aquatic	#River (Creek)
#Environmental	#Terrestrial	#Soil
#Condition	#Saline	-

What are isolation sources categories?

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #65488]	16S rRNA gene sequence	MF527111		GenBank

[Ref.: #65488]

GC-content

63 mol%

thermal denaturation, midpoint method (Tm)

Availability in culture collections External links

[Ref.: #65488]

Culture collection no.

CECT 9424, LMG 30322

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Roseovarius ramblicola sp. nov., a moderately halophilic bacterium isolated from saline soil in Spain.	Castro DJ, Cerezo I, Sampedro I, Martinez-Checa F	Int J Syst Evol Microbiol	10.1099/ijsem.0.002744	2018	*

References

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#65488

David J. Castro, Isabel Cerezo, Inmaculada Sampedro, Fernando Martínez-Checa: Roseovarius ramblicola sp. nov., a moderately halophilic bacterium isolated from saline soil in Spain. IJSEM 68: 1839 - 1844 2018 ( DOI 10.1099/ijsem.0.002744 , PubMed 29664362 )

* These data were automatically processed and therefore are not curated

Change proposal

You found an error in BacDive? Please tell us about it!

Note that changes will be reviewed and judged. If your changes are legitimate, changes will occur within the next BacDive update. Only proposed changes supported by the according reference will be reviewed. The BacDive team reserves the right to reject proposed changes.

Search for species Roseovarius ramblicola in external resources:
SILVA
BRENDA
PANGAEA
StrainInfo
GBIF