Name: Roseicyclus marinus MCCC 1K03242
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 158729

Type strain:

Species: Roseicyclus marinus

Culture col. no.: MCCC 1K03242, KCTC 52641, CCMM 001

Strain history: <- Lili Tang, Qingdao Institute of Bioenergy and Bioprocess

NCBI tax ID(s): 2161673 (species)

Section

Roseicyclus marinus MCCC 1K03242 is an aerobe, Gram-negative, motile bacterium that forms circular colonies and was isolated from an axenic Synechococcus culture with added bacteria from an offshore seawater sample.

colony-forming
Gram-negative
motile
rod-shaped
aerobe
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Pseudomonadota*
	Class	*Alphaproteobacteria*
	Order	*Rhodobacterales*
	Family	*Roseobacteraceae*
	Genus	*Roseicyclus*
	Species	**Roseicyclus marinus**
	Full Scientific Name (LPSN)	Roseicyclus marinus Tang et al. 2018

Information on morphological and physiological properties Morphology

[Ref.: #65479]	Gram stain	negative

[Ref.: #65479]	Cell length	1-2.5 µm

[Ref.: #65479]	Cell width	0.4-0.8 µm

[Ref.: #65479]	Cell shape	rod-shaped

[Ref.: #65479]	Motility	yes

[Ref.: #65479]	Flagellum arrangement	gliding

[Ref.: #65479]	Colony size	2-3 mm
[Ref.: #65479]	Colony color	pink
[Ref.: #65479]	Colony shape	circular
[Ref.: #65479]	Incubation period	2 days
[Ref.: #65479]	Cultivation medium used	marine agar

Information on culture and growth conditions Culture and growth conditions

[Ref.: #65479]

Culture medium

Marine agar (MA)

[Ref.: #65479]

Culture medium growth

[Ref.: #65479]

Culture medium

Mac-Conkey agar

[Ref.: #65479]

Culture medium growth

		Temperature
[Ref.: #65479]	growth	16-42 °C
[Ref.: #65479]	optimum	30 °C
[Ref.: #67771]	growth	28 °C

[Ref.: #65479]	Temperature range	mesophilic
[Ref.: #67771]	Temperature range	mesophilic

	pH	Kind of pH		pH
[Ref.: #65479]			optimum	7
[Ref.: #65479]			growth	6-10

Information on physiology and metabolism Physiology and metabolism

[Ref.: #65479]	Oxygen tolerance	aerobe
[Ref.: #67771]	Oxygen tolerance	aerobe

	Halophily	Salt	Tested relation		Salt conc.
[Ref.: #65479]		NaCl		optimum	0-9	%(w/v)
[Ref.: #65479]		NaCl		optimum	3	%(w/v)

[Ref.: #65479]	Observation	Cells contain BChl a
[Ref.: #65479]	Observation	major respiratory quinone is Q-10
[Ref.: #65479]	Observation	polar lipid profile comprises phosphatidylethanolamine, phosphatidylcholine, one unidentified aminolipid and five unidentified lipids

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #65479]	amygdalin ChEBI	-	builds acid from
[Ref.: #65479]	arbutin ChEBI	+	builds acid from
[Ref.: #65479]	casein	-	hydrolysis
[Ref.: #65479]	cellobiose ChEBI	-	builds acid from
[Ref.: #65479]	D-arabinose ChEBI	-	builds acid from
[Ref.: #65479]	D-arabitol ChEBI	-	builds acid from
[Ref.: #65479]	D-fructose ChEBI	-	builds acid from
[Ref.: #65479]	D-fructose ChEBI	-	carbon source
[Ref.: #65479]	D-fucose ChEBI	-	builds acid from
[Ref.: #65479]	D-galactose ChEBI	-	builds acid from
[Ref.: #65479]	D-glucose ChEBI	-	builds acid from
[Ref.: #65479]	D-glucose ChEBI	-	carbon source
[Ref.: #65479]	D-lyxose ChEBI	-	builds acid from
[Ref.: #65479]	D-mannitol ChEBI	-	builds acid from
[Ref.: #65479]	D-mannose ChEBI	-	builds acid from
[Ref.: #65479]	D-mannose ChEBI	+	carbon source
[Ref.: #65479]	D-ribose ChEBI	+	builds acid from
[Ref.: #65479]	D-sorbitol ChEBI	-	builds acid from
[Ref.: #65479]	D-tagatose ChEBI	-	builds acid from
[Ref.: #65479]	D-xylose ChEBI	-	builds acid from
[Ref.: #65479]	D-xylose ChEBI	-	carbon source
[Ref.: #65479]	erythritol ChEBI	-	builds acid from
[Ref.: #65479]	esculin ferric citrate	-	builds acid from
[Ref.: #65479]	galactitol ChEBI	-	builds acid from
[Ref.: #65479]	gentiobiose ChEBI	-	builds acid from
[Ref.: #65479]	glucose ChEBI	+	fermentation
[Ref.: #65479]	glycerol ChEBI	-	builds acid from
[Ref.: #65479]	glycogen ChEBI	+	builds acid from
[Ref.: #65479]	inulin ChEBI	-	builds acid from
[Ref.: #65479]	L-arabinose ChEBI	-	builds acid from
[Ref.: #65479]	L-arabitol ChEBI	-	builds acid from
[Ref.: #65479]	L-fucose ChEBI	-	builds acid from
[Ref.: #65479]	L-leucine ChEBI	-	carbon source
[Ref.: #65479]	L-lysine ChEBI	-	carbon source
[Ref.: #65479]	L-ornithine ChEBI	-	carbon source
[Ref.: #65479]	L-rhamnose ChEBI	-	builds acid from
[Ref.: #65479]	L-rhamnose ChEBI	+	carbon source
[Ref.: #65479]	L-sorbose ChEBI	-	builds acid from
[Ref.: #65479]	L-xylose ChEBI	-	builds acid from
[Ref.: #65479]	lactose ChEBI	-	builds acid from
[Ref.: #65479]	lactose ChEBI	-	carbon source
[Ref.: #65479]	maltose ChEBI	-	builds acid from
[Ref.: #65479]	melezitose ChEBI	-	builds acid from
[Ref.: #65479]	melibiose ChEBI	-	builds acid from
[Ref.: #65479]	methyl alpha-D-glucopyranoside ChEBI	-	builds acid from
[Ref.: #65479]	methyl alpha-D-mannoside ChEBI	-	builds acid from
[Ref.: #65479]	methyl beta-D-xylopyranoside ChEBI	-	builds acid from
[Ref.: #65479]	myo-inositol ChEBI	-	builds acid from
[Ref.: #65479]	N-acetylglucosamine ChEBI	+	builds acid from
[Ref.: #65479]	nitrate ChEBI	+	reduction
[Ref.: #65479]	potassium 2-dehydro-D-gluconate	-	builds acid from
[Ref.: #65479]	potassium 5-dehydro-D-gluconate	+	builds acid from
[Ref.: #65479]	potassium gluconate ChEBI	-	builds acid from
[Ref.: #65479]	raffinose ChEBI	-	builds acid from
[Ref.: #65479]	raffinose ChEBI	-	carbon source
[Ref.: #65479]	ribitol ChEBI	-	builds acid from
[Ref.: #65479]	salicin ChEBI	-	builds acid from
[Ref.: #65479]	sodium citrate ChEBI	-	carbon source
[Ref.: #65479]	starch ChEBI	-	builds acid from
[Ref.: #65479]	starch ChEBI	-	hydrolysis
[Ref.: #65479]	sucrose ChEBI	-	builds acid from
[Ref.: #65479]	sucrose ChEBI	-	carbon source
[Ref.: #65479]	trehalose ChEBI	+	builds acid from
[Ref.: #65479]	turanose ChEBI	-	builds acid from
[Ref.: #65479]	tween 20 ChEBI	-	hydrolysis
[Ref.: #65479]	tween 40 ChEBI	-	hydrolysis
[Ref.: #65479]	tween 80 ChEBI	-	hydrolysis
[Ref.: #65479]	xylitol ChEBI	-	builds acid from
	Only first 10 entries are displayed. Click here to see all.

	Metabolite production	Metabolite	Production
[Ref.: #65479]		sulfide ChEBI	no

	Enzyme	Enzyme activity	EC number
[Ref.: #65479]	acid phosphatase	-	3.1.3.2
[Ref.: #65479]	alkaline phosphatase	-	3.1.3.1
[Ref.: #65479]	alpha-chymotrypsin	-	3.4.21.1
[Ref.: #65479]	alpha-fucosidase	+	3.2.1.51
[Ref.: #65479]	alpha-galactosidase	-	3.2.1.22
[Ref.: #65479]	alpha-glucosidase	-	3.2.1.20
[Ref.: #65479]	alpha-mannosidase	-	3.2.1.24
[Ref.: #65479]	beta-galactosidase	+	3.2.1.23
[Ref.: #65479]	beta-glucosidase	-	3.2.1.21
[Ref.: #65479]	beta-glucuronidase	-	3.2.1.31
[Ref.: #65479]	catalase	-	1.11.1.6
[Ref.: #65479]	cystine arylamidase	+	3.4.11.3
[Ref.: #65479]	cytochrome oxidase	+	1.9.3.1
[Ref.: #65479]	DNase	-
[Ref.: #65479]	esterase Lipase (C 8)	-
[Ref.: #65479]	gelatinase	-
[Ref.: #65479]	leucine arylamidase	-	3.4.11.1
[Ref.: #65479]	lipase (C 14)	-
[Ref.: #65479]	N-acetyl-beta-glucosaminidase	-	3.2.1.52
[Ref.: #65479]	naphthol-AS-BI-phosphohydrolase	-
[Ref.: #65479]	trypsin	-	3.4.21.4
[Ref.: #65479]	valine arylamidase	-
	Only first 10 entries are displayed. Click here to see all.

Fatty acid profile

Metadata FA analysis

Reference

[Ref.: #65479]

Fatty acid	Percentage
C18:1ω7c	55.2
C19:0cycloω8c	10.1
C16:0	8
C16:0 2OH	6.1
11 methyl C18:1ω7c	5.6
7 (ECL 18.846/C19:1ω6c)	3.9
ECL 11.799	3.7
C18:0	1.9
C18:1 2OH	1.6
C18:1ω9c	1.2

Metadata FA analysis

Reference

[Ref.: #65479]

Fatty acid	Percentage
C18:1ω7c	36.9
C19:0 cycloω8c	22.1
C16:0	7.5
11 methyl C18:1ω7c	6.9
C16:0 2OH	6.6
C10:0 3OH	5.1
7 (ECL 18.846/C19:1ω6c)	4.2
ECL 11.799	3.9
C18:1 2OH	1.7
C18:0	1.7
C18:1ω9c	1.2

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #65479]	Sample type/isolated from	an axenic Synechococcus culture with added bacteria from an offshore seawater sample
[Ref.: #65479]	Geographic location (country and/or sea, region)	Qingdao
[Ref.: #65479]	Country	China
[Ref.: #65479]	Country ISO 3 Code	CHN
[Ref.: #65479]	Continent	Asia
[Ref.: #65479]	Geographic location	36.0833°/120.4830°
[Ref.: #65479]	Isolation date	September 20
[Ref.: #65479]	Isolation/enrichment procedure	mixed culture was maintained by transferring small quantities (1 ml) of culture to fresh axenic Synechococcus culture every 8-12 days and incubated at 26 C under a 12:12 h light:dark cycle at approximately 50 µE m^-2 s^-1. To isolate bacteria that were closely related to Synechococcus species PCC7002, after five transferring steps, the culture was spread onto marine agar 2216 (MA; Becton Dickinson) plates, and bacterial colonies were isolated using the serial dilution method

[Ref.: #67771]	Sample type/isolated from	From seawater
[Ref.: #67771]	Country	China
[Ref.: #67771]	Country ISO 3 Code	CHN
[Ref.: #67771]	Continent	Asia
* marker position based on {}

Isolation sources categories

#Engineered	#Laboratory	#Lab enrichment
#Environmental	#Aquatic	#Marine
#Engineered	#Laboratory	#Simulated communities

What are isolation sources categories?

[Ref.: #69479]

Global distribution of 16S sequence KY060008 (>99% sequence identity) for Roseicyclus marinus subclade from Microbeatlas

Aquatic Samples	81
Soil Samples
Animal Samples	35
Plant Samples	1
Total Samples	117

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #65479]	16S rRNA gene sequence	KY060008		GenBank

Genome sequence information:

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #66792]	Roseicyclus marinus CCMM001	GCA_029589195	contig	GenBank	2161673 tax ID	*

[Ref.: #65479]

GC-content

63.5 mol%

high performance liquid chromatography (HPLC)

Data predicted using genome information as a basis Genome-based predictions

	Trait	Prediction	Confidence in %	In training data
	Predictions from Diaspora project based on: Roseicyclus marinus CCMM001 NCBI: GCA_029589195.1
[Ref.: #69480]	motile	no	86.647	no
[Ref.: #69480]	flagellated	no	94.913	yes
[Ref.: #69480]	gram-positive	no	85.668	no
[Ref.: #69480]	anaerobic	no	96.115	no
[Ref.: #69480]	aerobic	yes	64.687	yes
[Ref.: #69480]	halophile	no	65.388	no
[Ref.: #69480]	spore-forming	no	95.006	no
[Ref.: #69480]	glucose-ferment	yes	71.576	yes
[Ref.: #69480]	thermophile	no	98.244	no
[Ref.: #69480]	glucose-util	yes	82.83	no

Availability in culture collections External links

[Ref.: #65479]

Culture collection no.

MCCC 1K03242, KCTC 52641, CCMM 001

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Roseicyclus marinus sp. nov., isolated from a Synechococcus culture, and emended description of the genus Roseicyclus.	Tang L, Zhang Z, Zhou C, Cui R, Tian Y, Zhang Y	Int J Syst Evol Microbiol	10.1099/ijsem.0.002752	2018	*

References

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#65479

Lili Tang, Zenghu Zhang, Chao Zhou, Rong Cui, Yu Tian, Yongyu Zhang: Roseicyclus marinus sp. nov., isolated from a Synechococcus culture, and emended description of the genus Roseicyclus. IJSEM 68: 1781 - 1786 2018 ( DOI 10.1099/ijsem.0.002752 , PubMed 29620503 )

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#67771 Korean Collection for Type Cultures (KCTC) ; Curators of the KCTC;

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

#69480

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

* These data were automatically processed and therefore are not curated

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Roseicyclus marinus CCMM001

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